Rh2DG086200

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0022677
Physical Location & Seq
Forward (+)
6689938 .. 6691173
1236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG086200.1

Sequence Viewer

Length: 975 bp
ATGATAGTCCCAATTGTGGTTGTTACAGCTGCTGGAGGTTATGGTAAAACCCTCCTTGCCTTAACATTAAGCCAAGATAAAGAAGTGAGAGATACGGTCCGCAACAATATCATCTTTATCAGTTTTCCAAAGAAGGGAAATTTGCAGTGTATCGTACAACGATTGTGTAAACACAAAGGATCAGGACACCGCAAGATTTTGGTGACGTCAAGATTCGAAATTCCAAGATTTGGTATTCCCTTTCATCTATATGGGTTGTCTGAGGACGAAGCTATGGCACTGCTCTGTCATTCAGCAGGCTTGGCATACTTTGATAAGGAAGACAGAAACAAATCTCGTATGCGTGAAGGCATGACCGAGTTCACAACAGAAGCCCGGCAGATAGTAAGCCATTGTTCCGGATCTCCCCTTGCCATAACAGTGGTGGGGAAATCGCTTTGTGGGAAACCTGCACACGTTTGGAGAAGGAAAGCATTCGAATTGGATTATCATAATACTATTTTAGACACTGACATTACTTTGTTGTCGTGCCTCCAAAGTACTTTTGAAGACATGGATAAAGAACTCCAAGCTGCATACTTGGACCTTGCCGTACTCCCAAATAAATTTTTGGACTGTCTTGGTACTGCAGTTGATGTATGGGCAGAGTTGCAGAGTCATCTGCTTGATAATGTCCTTTTCTGCACTACAACTTTCCACGAACTGAGCGCCTGGCATTTGGTATTTCTTCAATACTCTTCGGATAAAAGGTTCTTTCAGAATGAGAGAATAGATGATTACTGGTTCCGTCATGTTCGACTTGTTCAGAGAGTATGCTTGCTGCCTAGCCAGTTTGGATCCAATAGAAGAGAGACAGAGATTGATTTTAGAAGTATTTGGAAAAAATATTCCCAAGTGGTTGAGAGAACTGAAGGATCAGCCATTGAAGGCGCAGCTACTGTCTATCTCAACAGGAATGTTGTGTGTGGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

324

Amino Acids

36.96

Weight (kDa)

8.28

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022677)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0093951
rosa_roxburghii Rroxscaffold_2G00147580
rosa_samantha Rh2AG087600 Rh2DG086200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 457
AccB7I CCANNNNNTGG 1 cut(s) 230
AccIII TCCGGA 1 cut(s) 398
AciI CCGC 2 cut(s) 100, 190
AclWI GGATC 5 cut(s) 187, 409, 831, 844, 922
AcsI RAATTY 3 cut(s) 139, 219, 605
AcuI CTGAAG 1 cut(s) 930
AcyI GRCGYC 1 cut(s) 206
AfaI GTAC 4 cut(s) 156, 541, 594, 625
AfiI CCNNNNNNNGG 3 cut(s) 16, 134, 230
AflIII ACRYGT 1 cut(s) 454
AgsI TTSAA 3 cut(s) 548, 731, 926
AjnI CCWGG 1 cut(s) 710
AloI GAACNNNNNNTCC 2 cut(s) 734, 766
AluBI AGCT 4 cut(s) 29, 272, 572, 935
AluI AGCT 4 cut(s) 29, 272, 572, 935
Alw26I GTCTC 1 cut(s) 845
AlwI GGATC 5 cut(s) 187, 409, 831, 844, 922
AlwNI CAGNNNCTG 2 cut(s) 32, 938
Aor13HI TCCGGA 1 cut(s) 398
ApeKI GCWGC 4 cut(s) 29, 572, 820, 932
ApoI RAATTY 3 cut(s) 139, 219, 605
Asp700I GAANNNNTTC 1 cut(s) 473
AspLEI GCGC 2 cut(s) 710, 932
AspS9I GGNCC 2 cut(s) 97, 583
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 1 cut(s) 214
AsuII TTCGAA 2 cut(s) 216, 477
AvaII GGWCC 2 cut(s) 97, 583
BaeI ACNNNNGTAYC 2 cut(s) 615, 648
BamHI GGATCC 1 cut(s) 836
BbsI GAAGAC 2 cut(s) 327, 555
BbvI GCAGC 4 cut(s) 16, 559, 807, 944
BceAI ACGGC 1 cut(s) 575
BciT130I CCWGG 1 cut(s) 712
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 1 cut(s) 845
BfaI CTAG 1 cut(s) 825
BfmI CTRYAG 1 cut(s) 627
BfoI RGCGCY 1 cut(s) 711
BfuAI ACCTGC 1 cut(s) 457
BisI GCNGC 4 cut(s) 30, 573, 821, 933
BlsI GCNGC 4 cut(s) 31, 574, 822, 934
BmcAI AGTACT 1 cut(s) 541
Bme1390I CCNGG 2 cut(s) 376, 712
Bme18I GGWCC 2 cut(s) 97, 583
BmgT120I GGNCC 2 cut(s) 97, 583
BmiI GGNNCC 2 cut(s) 785, 838
BmrFI CCNGG 2 cut(s) 376, 712
BpiI GAAGAC 2 cut(s) 327, 555
BpmI CTGGAG 1 cut(s) 54
Bpu14I TTCGAA 2 cut(s) 216, 477
BpuMI CCSGG 1 cut(s) 376
BsaHI GRCGYC 1 cut(s) 206
BsaWI WCCGGW 1 cut(s) 398
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 134, 230
Bse1I ACTGG 2 cut(s) 785, 829
BseAI TCCGGA 1 cut(s) 398
BseBI CCWGG 1 cut(s) 712
BseLI CCNNNNNNNGG 3 cut(s) 16, 134, 230
BseMII CTCAG 2 cut(s) 252, 695
BseNI ACTGG 2 cut(s) 785, 829
BseXI GCAGC 4 cut(s) 16, 559, 807, 944
BsgI GTGCAG 2 cut(s) 435, 667
BsiSI CCGG 2 cut(s) 376, 399
BslI CCNNNNNNNGG 3 cut(s) 16, 134, 230
BsmAI GTCTC 1 cut(s) 845
BsmI GAATGC 1 cut(s) 473
Bsp119I TTCGAA 2 cut(s) 216, 477
Bsp13I TCCGGA 1 cut(s) 398
Bsp143I GATC 4 cut(s) 179, 401, 836, 914
BspACI CCGC 2 cut(s) 100, 190
BspCNI CTCAG 2 cut(s) 253, 696
BspEI TCCGGA 1 cut(s) 398
BspLI GGNNCC 2 cut(s) 785, 838
BspMAI CTGCAG 1 cut(s) 631
BspMI ACCTGC 1 cut(s) 457
BspPI GGATC 5 cut(s) 187, 409, 831, 844, 922
BspT104I TTCGAA 2 cut(s) 216, 477
BsrI ACTGG 2 cut(s) 785, 829
BssMI GATC 4 cut(s) 179, 401, 836, 914
BssNI GRCGYC 1 cut(s) 206
Bst2UI CCWGG 1 cut(s) 712
Bst4CI ACNGT 4 cut(s) 97, 421, 617, 940
Bst6I CTCTTC 2 cut(s) 742, 841
BstACI GRCGYC 1 cut(s) 206
BstBI TTCGAA 2 cut(s) 216, 477
BstC8I GCNNGC 2 cut(s) 298, 818
BstDEI CTNAG 2 cut(s) 261, 704
BstH2I RGCGCY 1 cut(s) 711
BstHHI GCGC 2 cut(s) 710, 932
BstKTI GATC 4 cut(s) 182, 404, 839, 917
BstMAI GTCTC 1 cut(s) 845
BstMBI GATC 4 cut(s) 179, 401, 836, 914
BstMWI GCNNNNNNNGC 1 cut(s) 302
BstNI CCWGG 1 cut(s) 712
BstSCI CCNGG 2 cut(s) 374, 710
BstSFI CTRYAG 1 cut(s) 627
BstV1I GCAGC 4 cut(s) 16, 559, 807, 944
BstV2I GAAGAC 2 cut(s) 327, 555
BstX2I RGATCY 2 cut(s) 401, 836
BstXI CCANNNNNNTGG 1 cut(s) 421
BstYI RGATCY 2 cut(s) 401, 836
BtsI GCAGTG 2 cut(s) 152, 278
BtsIMutI CAGTG 4 cut(s) 152, 278, 426, 507
BveI ACCTGC 1 cut(s) 457
Cac8I GCNNGC 2 cut(s) 298, 818
CaiI CAGNNNCTG 2 cut(s) 32, 938
CfoI GCGC 2 cut(s) 710, 932
Cfr13I GGNCC 2 cut(s) 97, 583
CpoI CGGWCCG 1 cut(s) 97
Csp6I GTAC 4 cut(s) 155, 540, 593, 624
CspI CGGWCCG 1 cut(s) 97
CviAII CATG 3 cut(s) 352, 553, 791
CviQI GTAC 4 cut(s) 155, 540, 593, 624
DdeI CTNAG 2 cut(s) 261, 704
DpnI GATC 4 cut(s) 181, 403, 838, 916
DpnII GATC 4 cut(s) 179, 401, 836, 914
Eam1104I CTCTTC 2 cut(s) 742, 841
EarI CTCTTC 2 cut(s) 742, 841
Eco47I GGWCC 2 cut(s) 97, 583
Eco57I CTGAAG 1 cut(s) 930
EcoRII CCWGG 1 cut(s) 710
FaeI CATG 3 cut(s) 355, 556, 794
FatI CATG 3 cut(s) 351, 552, 790
Fnu4HI GCNGC 4 cut(s) 30, 573, 821, 933
Fsp4HI GCNGC 4 cut(s) 30, 573, 821, 933
FspBI CTAG 1 cut(s) 825
GlaI GCGC 2 cut(s) 709, 931
GluI GCNGC 4 cut(s) 30, 573, 821, 933
GsuI CTGGAG 1 cut(s) 54
HaeII RGCGCY 1 cut(s) 711
HapII CCGG 2 cut(s) 376, 399
HhaI GCGC 2 cut(s) 710, 932
Hin1I GRCGYC 1 cut(s) 206
Hin1II CATG 3 cut(s) 355, 556, 794
Hin6I GCGC 2 cut(s) 708, 930
HinP1I GCGC 2 cut(s) 708, 930
HinfI GANTC 2 cut(s) 213, 655
HpaII CCGG 2 cut(s) 376, 399
HphI GGTGA 1 cut(s) 214
Hpy166II GTNNAC 2 cut(s) 170, 363
Hpy188I TCNGA 4 cut(s) 262, 742, 759, 807
Hpy188III TCNNGA 3 cut(s) 183, 210, 399
Hpy8I GTNNAC 2 cut(s) 170, 363
HpyAV CCTTC 5 cut(s) 127, 341, 459, 905, 920
HpyCH4III ACNGT 4 cut(s) 97, 421, 617, 940
HpyCH4IV ACGT 2 cut(s) 206, 456
HpyCH4V TGCA 6 cut(s) 145, 452, 575, 629, 652, 684
HpyF10VI GCNNNNNNNGC 1 cut(s) 302
HpyF3I CTNAG 2 cut(s) 261, 704
HpySE526I ACGT 2 cut(s) 206, 456
Hsp92I GRCGYC 1 cut(s) 206
Hsp92II CATG 3 cut(s) 355, 556, 794
HspAI GCGC 2 cut(s) 708, 930
Kpn2I TCCGGA 1 cut(s) 398
Kzo9I GATC 4 cut(s) 179, 401, 836, 914
Lsp1109I GCAGC 4 cut(s) 16, 559, 807, 944
MaeI CTAG 1 cut(s) 825
MaeII ACGT 2 cut(s) 206, 456
MaeIII GTNAC 2 cut(s) 22, 202
MalI GATC 4 cut(s) 181, 403, 838, 916
MboI GATC 4 cut(s) 179, 401, 836, 914
MboII GAAGA 5 cut(s) 332, 560, 719, 729, 858
MfeI CAATTG 1 cut(s) 12
MflI RGATCY 2 cut(s) 401, 836
MluCI AATT 5 cut(s) 12, 139, 219, 479, 605
MlyI GAGTC 1 cut(s) 664
MnlI CCTC 4 cut(s) 29, 62, 256, 542
MroI TCCGGA 1 cut(s) 398
MroXI GAANNNNTTC 1 cut(s) 473
MseI TTAA 2 cut(s) 62, 68
MslI CAYNNNNRTG 2 cut(s) 249, 419
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 2 cut(s) 376, 399
MspR9I CCNGG 2 cut(s) 376, 712
MunI CAATTG 1 cut(s) 12
Mva1269I GAATGC 1 cut(s) 473
MvaI CCWGG 1 cut(s) 712
MwoI GCNNNNNNNGC 1 cut(s) 302
NciI CCSGG 1 cut(s) 376
NdeII GATC 4 cut(s) 179, 401, 836, 914
NlaIII CATG 3 cut(s) 355, 556, 794
NlaIV GGNNCC 2 cut(s) 785, 838
NmuCI GTSAC 1 cut(s) 202
NspV TTCGAA 2 cut(s) 216, 477
PctI GAATGC 1 cut(s) 473
PdmI GAANNNNTTC 1 cut(s) 473
PfeI GAWTC 1 cut(s) 213
PflMI CCANNNNNTGG 1 cut(s) 230
PkrI GCNGC 4 cut(s) 31, 574, 822, 934
PleI GAGTC 1 cut(s) 663
PpsI GAGTC 1 cut(s) 663
Psp6I CCWGG 1 cut(s) 710
PspGI CCWGG 1 cut(s) 710
PspN4I GGNNCC 2 cut(s) 785, 838
PspPI GGNCC 2 cut(s) 97, 583
PstI CTGCAG 1 cut(s) 631
PstNI CAGNNNCTG 2 cut(s) 32, 938
PsuI RGATCY 2 cut(s) 401, 836
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 4 cut(s) 156, 541, 594, 625
RsaNI GTAC 4 cut(s) 155, 540, 593, 624
RseI CAYNNNNRTG 2 cut(s) 249, 419
Rsr2I CGGWCCG 1 cut(s) 97
RsrII CGGWCCG 1 cut(s) 97
SaqAI TTAA 2 cut(s) 62, 68
SatI GCNGC 4 cut(s) 30, 573, 821, 933
Sau3AI GATC 4 cut(s) 179, 401, 836, 914
Sau96I GGNCC 2 cut(s) 97, 583
ScaI AGTACT 1 cut(s) 541
SchI GAGTC 1 cut(s) 664
ScrFI CCNGG 2 cut(s) 376, 712
SfcI CTRYAG 1 cut(s) 627
SfuI TTCGAA 2 cut(s) 216, 477
SinI GGWCC 2 cut(s) 97, 583
SmiMI CAYNNNNRTG 2 cut(s) 249, 419
Sse9I AATT 5 cut(s) 12, 139, 219, 479, 605
SsiI CCGC 2 cut(s) 100, 190
SspI AATATT 1 cut(s) 887
SspMI CTAG 1 cut(s) 825
StyD4I CCNGG 2 cut(s) 374, 710
TaaI ACNGT 4 cut(s) 97, 421, 617, 940
TaiI ACGT 2 cut(s) 209, 459
TaqI TCGA 3 cut(s) 216, 477, 796
TaqII GACCGA 1 cut(s) 371
TasI AATT 5 cut(s) 12, 139, 219, 479, 605
TatI WGTACW 1 cut(s) 539
TfiI GAWTC 1 cut(s) 213
Tru1I TTAA 2 cut(s) 62, 68
Tru9I TTAA 2 cut(s) 62, 68
TscAI CASTG 4 cut(s) 152, 285, 426, 514
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 4 cut(s) 29, 572, 820, 932
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 1 cut(s) 233
TspGWI ACGGA 1 cut(s) 776
TspRI CASTG 4 cut(s) 152, 285, 426, 514
Van91I CCANNNNNTGG 1 cut(s) 230
VpaK11BI GGWCC 2 cut(s) 97, 583
XapI RAATTY 3 cut(s) 139, 219, 605
XcmI CCANNNNNNNNNTGG 1 cut(s) 421
XmnI GAANNNNTTC 1 cut(s) 473
XspI CTAG 1 cut(s) 825
ZraI GACGTC 1 cut(s) 207
ZrmI AGTACT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.