Rh2DG293200

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0019874
Physical Location & Seq
Forward (+)
34501370 .. 34501942
573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG293200.1

Sequence Viewer

Length: 573 bp
ATGTTGTCTGGAGAACGAAAAAAATCGTTCACATCTTGGCGGTCCATCAATAGCGACGGTGATGGTAATCAGTTGATCATCTCTGTTCATCCCGATGATAAACTGAAATTTCTAGTTGAGCGAAGAAAGCAAAGCTCTTGTAATCTAAACGTTTTCAATAACACAGAACACTGTGTTGCGTTCAAGATTAAAACCACAAACCCTAAGAAGTACATTGCAAATCCCAAGTCTGGTGTTATACAGGCTCGGCACTCATGTGTCATCAGACTCACCCAGCAAGTCATCGTTAAATATCCTCTAACTGGGGAGTGCAAAGACAAATACCTCATACAGACTACAATAGTGCCTCCAAATTATGCTGCTGATGAGCTTCCGAAAGATACTTTTGCCAAGGAAAGTGGAAGGAGAATAGAGGAGTGCAAGCTTCCAGTTGTGTATATCATTCCTCCAGATTTGGGTCACCAGGAACATGATATATGGAGCTTGCTAAGAGATAGTGTCAAAGATGGCAAAATACCGGTAACTGAACCAAGCCCAGACCGTGACCTGACGGTGACTTTGTTGCAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

190

Amino Acids

21.58

Weight (kDa)

8.92

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 27 - 127 5.8e-21 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019874)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0117201
rosa_multiflora Rmu_sc0004602.1_g000045
rosa_roxburghii Rroxscaffold_2G00126650
rosa_rugosa Rorug02G0210800
rosa_samantha Rh2BG279100 Rh2DG273800 Rh2DG293200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 40
AclI AACGTT 1 cut(s) 150
AcsI RAATTY 1 cut(s) 107
AfaI GTAC 1 cut(s) 212
AfiI CCNNNNNNNGG 3 cut(s) 230, 302, 455
AgeI ACCGGT 1 cut(s) 517
AgsI TTSAA 2 cut(s) 157, 184
AjnI CCWGG 1 cut(s) 462
AleI CACNNNNGTG 1 cut(s) 255
AluBI AGCT 4 cut(s) 135, 370, 424, 483
AluI AGCT 4 cut(s) 135, 370, 424, 483
ApeKI GCWGC 2 cut(s) 359, 565
ApoI RAATTY 1 cut(s) 107
ArsI GACNNNNNNTTYG 4 cut(s) 212, 244, 541, 573
AsiGI ACCGGT 1 cut(s) 517
AspS9I GGNCC 1 cut(s) 42
AsuHPI GGTGA 4 cut(s) 71, 262, 452, 565
AvaII GGWCC 1 cut(s) 42
BbvI GCAGC 1 cut(s) 346
BccI CCATC 3 cut(s) 53, 56, 500
BciT130I CCWGG 1 cut(s) 464
BclI TGATCA 1 cut(s) 75
BfaI CTAG 1 cut(s) 113
BisI GCNGC 2 cut(s) 360, 566
BlsI GCNGC 2 cut(s) 361, 567
Bme1390I CCNGG 1 cut(s) 464
Bme18I GGWCC 1 cut(s) 42
BmgT120I GGNCC 1 cut(s) 42
BmrFI CCNGG 1 cut(s) 464
BmrI ACTGGG 1 cut(s) 312
BmuI ACTGGG 1 cut(s) 312
BpmI CTGGAG 2 cut(s) 30, 432
BsaBI GATNNNNATC 1 cut(s) 66
BsaJI CCNNGG 1 cut(s) 390
BsaWI WCCGGW 1 cut(s) 517
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 302, 455
Bse118I RCCGGY 1 cut(s) 517
Bse1I ACTGG 2 cut(s) 307, 428
Bse3DI GCAATG 1 cut(s) 213
Bse8I GATNNNNATC 1 cut(s) 66
BseBI CCWGG 1 cut(s) 464
BseDI CCNNGG 1 cut(s) 390
BseGI GGATG 1 cut(s) 88
BseJI GATNNNNATC 1 cut(s) 66
BseLI CCNNNNNNNGG 3 cut(s) 230, 302, 455
BseMI GCAATG 1 cut(s) 213
BseNI ACTGG 2 cut(s) 307, 428
BseRI GAGGAG 1 cut(s) 428
BseXI GCAGC 1 cut(s) 346
BseYI CCCAGC 1 cut(s) 273
BshTI ACCGGT 1 cut(s) 517
BsiSI CCGG 1 cut(s) 518
BslI CCNNNNNNNGG 3 cut(s) 230, 302, 455
Bsp143I GATC 1 cut(s) 75
BspACI CCGC 1 cut(s) 40
BsrDI GCAATG 1 cut(s) 213
BsrFI RCCGGY 1 cut(s) 517
BsrI ACTGG 2 cut(s) 307, 428
BssAI RCCGGY 1 cut(s) 517
BssECI CCNNGG 1 cut(s) 390
BssMI GATC 1 cut(s) 75
BssT1I CCWWGG 1 cut(s) 390
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 4 cut(s) 59, 173, 542, 553
BstC8I GCNNGC 2 cut(s) 422, 485
BstDEI CTNAG 2 cut(s) 204, 488
BstEII GGTNACC 1 cut(s) 458
BstF5I GGATG 1 cut(s) 88
BstKTI GATC 1 cut(s) 78
BstMBI GATC 1 cut(s) 75
BstMWI GCNNNNNNNGC 1 cut(s) 127
BstNI CCWGG 1 cut(s) 464
BstPI GGTNACC 1 cut(s) 458
BstSCI CCNGG 1 cut(s) 462
BstV1I GCAGC 1 cut(s) 346
BtsCI GGATG 1 cut(s) 88
BtsIMutI CAGTG 1 cut(s) 169
Cac8I GCNNGC 2 cut(s) 422, 485
Cfr10I RCCGGY 1 cut(s) 517
Cfr13I GGNCC 1 cut(s) 42
Csp6I GTAC 1 cut(s) 211
CspAI ACCGGT 1 cut(s) 517
CspCI CAANNNNNGTGG 2 cut(s) 379, 414
CviAII CATG 2 cut(s) 255, 470
CviJI RGCY 6 cut(s) 135, 245, 370, 424, 483, 534
CviKI_1 RGCY 6 cut(s) 135, 245, 370, 424, 483, 534
CviQI GTAC 1 cut(s) 211
DdeI CTNAG 2 cut(s) 204, 488
DpnI GATC 1 cut(s) 77
DpnII GATC 1 cut(s) 75
Eco130I CCWWGG 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 42
Eco91I GGTNACC 1 cut(s) 458
EcoO65I GGTNACC 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 462
EcoT14I CCWWGG 1 cut(s) 390
ErhI CCWWGG 1 cut(s) 390
FaeI CATG 2 cut(s) 258, 473
FaiI YATR 8 cut(s) 239, 256, 329, 357, 438, 471, 476, 478
FatI CATG 2 cut(s) 254, 469
FbaI TGATCA 1 cut(s) 75
Fnu4HI GCNGC 2 cut(s) 360, 566
FokI GGATG 1 cut(s) 75
Fsp4HI GCNGC 2 cut(s) 360, 566
FspBI CTAG 1 cut(s) 113
GluI GCNGC 2 cut(s) 360, 566
GsaI CCCAGC 1 cut(s) 277
GsuI CTGGAG 2 cut(s) 30, 432
HapII CCGG 1 cut(s) 518
Hin1II CATG 2 cut(s) 258, 473
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 1 cut(s) 267
HpaII CCGG 1 cut(s) 518
HphI GGTGA 4 cut(s) 71, 262, 452, 565
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 2 cut(s) 266, 375
Hpy188III TCNNGA 4 cut(s) 9, 92, 184, 449
Hpy8I GTNNAC 1 cut(s) 30
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 1 cut(s) 396
HpyCH4III ACNGT 4 cut(s) 59, 173, 542, 553
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 4 cut(s) 218, 312, 420, 565
HpyF10VI GCNNNNNNNGC 1 cut(s) 127
HpyF3I CTNAG 2 cut(s) 204, 488
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 2 cut(s) 258, 473
Ksp22I TGATCA 1 cut(s) 75
Kzo9I GATC 1 cut(s) 75
LmnI GCTCC 1 cut(s) 480
Lsp1109I GCAGC 1 cut(s) 346
MaeI CTAG 1 cut(s) 113
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 4 cut(s) 458, 520, 542, 553
MalI GATC 1 cut(s) 77
MboI GATC 1 cut(s) 75
MboII GAAGA 1 cut(s) 135
MluCI AATT 2 cut(s) 107, 352
MlyI GAGTC 1 cut(s) 261
MnlI CCTC 5 cut(s) 306, 335, 357, 406, 456
MseI TTAA 2 cut(s) 189, 288
MslI CAYNNNNRTG 2 cut(s) 93, 255
MspI CCGG 1 cut(s) 518
MspR9I CCNGG 1 cut(s) 464
MvaI CCWGG 1 cut(s) 464
MwoI GCNNNNNNNGC 1 cut(s) 127
NdeII GATC 1 cut(s) 75
NlaIII CATG 2 cut(s) 258, 473
NmeAIII GCCGAG 1 cut(s) 226
NmuCI GTSAC 3 cut(s) 458, 542, 553
OliI CACNNNNGTG 1 cut(s) 255
PinAI ACCGGT 1 cut(s) 517
PkrI GCNGC 2 cut(s) 361, 567
PleI GAGTC 1 cut(s) 261
PpsI GAGTC 1 cut(s) 261
Psp1406I AACGTT 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 462
PspEI GGTNACC 1 cut(s) 458
PspFI CCCAGC 1 cut(s) 273
PspGI CCWGG 1 cut(s) 462
PspPI GGNCC 1 cut(s) 42
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
RseI CAYNNNNRTG 2 cut(s) 93, 255
SaqAI TTAA 2 cut(s) 189, 288
SatI GCNGC 2 cut(s) 360, 566
Sau3AI GATC 1 cut(s) 75
Sau96I GGNCC 1 cut(s) 42
SchI GAGTC 1 cut(s) 261
ScrFI CCNGG 1 cut(s) 464
SetI ASST 7 cut(s) 137, 153, 327, 372, 426, 485, 549
SinI GGWCC 1 cut(s) 42
SmiMI CAYNNNNRTG 2 cut(s) 93, 255
Sse9I AATT 2 cut(s) 107, 352
SsiI CCGC 1 cut(s) 40
SspMI CTAG 1 cut(s) 113
StyD4I CCNGG 1 cut(s) 462
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 4 cut(s) 59, 173, 542, 553
TaiI ACGT 1 cut(s) 153
TasI AATT 2 cut(s) 107, 352
TatI WGTACW 1 cut(s) 210
Tru1I TTAA 2 cut(s) 189, 288
Tru9I TTAA 2 cut(s) 189, 288
TscAI CASTG 1 cut(s) 176
TseFI GTSAC 3 cut(s) 458, 542, 553
TseI GCWGC 2 cut(s) 359, 565
Tsp45I GTSAC 3 cut(s) 458, 542, 553
TspDTI ATGAA 1 cut(s) 77
TspRI CASTG 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 42
XapI RAATTY 1 cut(s) 107
XspI CTAG 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.