Rh2DG399300

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0022714
Physical Location & Seq
Reverse (-)
58153172 .. 58164564
11393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG399300.1

Sequence Viewer

Length: 660 bp
ATGTGCAGCATGCAAAGAGTATGGGTTAATCACCCATTGACCAATTCTCCCTTGACACGTGGCATTCATTCTTCTTTCCTGCCGAACCAAGCAGCACACGAGCAGGATGACAAGAAGGCCCCTGACTCTGCTGCTAAAGTCAAAGGCGAATCAGCCAAGAAGGGATATGTTGGAATTCACATCTCAGGATTCAGAGATATCCTTTTAAGGCCGGAGCTTGTACCCCCGATTGTGGACTCAATATTTGAGCATCCTTTTGAAGGTATACTATTGAATTCAATCTTCCCCGGCGACGGTATGGCCCCGAGATTGCTGAGTCCGTCAAACAGATGGATGGAGTACCCAGCTCATGCAAGAACTTCGAGACATCCCTTGGATCAGTCGTTGGCTACATATGGAGACCATTCAGTCCTACGCGAAAAATGCTTATCTAAAGCTGTAGTTGCTTCTGAACTCAAAGAAGGGGTTTTTCAATTTCTCTGGGCGCATCGACGATTTTGCTGTTTCTCTGTGAGGAGAGTATTTGAGGAAAGAATGGAGAGTGAGGAGGGGGATGAAGCAAAGGTTGCGATCGGACATGACGCCCTTGATCGTATGGTTGTGGTCGACACTAGAGTAGCCGAGCTCGAGGGCCTTGGTCACAAGCTTTGTGCGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

219

Amino Acids

24.48

Weight (kDa)

6.59

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022714)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0399031 RchiOBHm_Chr5g0070511 RchiOBHm_Chr6g0295231
rosa_samantha Rh2DG399300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 407
AccI GTMKAC 2 cut(s) 265, 606
AccII CGCG 1 cut(s) 417
AciI CCGC 1 cut(s) 653
AclWI GGATC 1 cut(s) 384
AcsI RAATTY 2 cut(s) 174, 274
AcvI CACGTG 1 cut(s) 59
AcyI GRCGYC 1 cut(s) 582
AfaI GTAC 2 cut(s) 222, 341
AfiI CCNNNNNNNGG 3 cut(s) 232, 260, 293
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 4 cut(s) 260, 274, 279, 473
AluBI AGCT 5 cut(s) 217, 347, 437, 625, 646
AluI AGCT 5 cut(s) 217, 347, 437, 625, 646
Alw21I GWGCWC 1 cut(s) 627
Alw26I GTCTC 2 cut(s) 358, 393
AlwI GGATC 1 cut(s) 384
Ama87I CYCGRG 2 cut(s) 304, 626
AoxI GGCC 4 cut(s) 117, 209, 300, 631
ApeKI GCWGC 3 cut(s) 6, 92, 131
ApoI RAATTY 2 cut(s) 174, 274
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
AspLEI GCGC 1 cut(s) 487
AspS9I GGNCC 3 cut(s) 118, 301, 631
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 1 cut(s) 23
AvaI CYCGRG 2 cut(s) 304, 626
BanII GRGCYC 1 cut(s) 627
BauI CACGAG 1 cut(s) 98
BbrPI CACGTG 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 627
BbvI GCAGC 3 cut(s) 18, 104, 118
BccI CCATC 2 cut(s) 324, 328
BcgI CGANNNNNNTGC 4 cut(s) 342, 376, 480, 514
BcnI CCSGG 1 cut(s) 288
BcoDI GTCTC 2 cut(s) 358, 393
BfaI CTAG 1 cut(s) 612
BfmI CTRYAG 1 cut(s) 438
BisI GCNGC 3 cut(s) 7, 93, 132
BlsI GCNGC 3 cut(s) 8, 94, 133
Bme1390I CCNGG 1 cut(s) 288
BmeT110I CYCGRG 2 cut(s) 304, 626
BmgT120I GGNCC 3 cut(s) 118, 301, 631
BmiI GGNNCC 2 cut(s) 120, 303
BmrFI CCNGG 1 cut(s) 288
BmsI GCATC 2 cut(s) 259, 496
BpuMI CCSGG 1 cut(s) 288
BsaAI YACGTR 1 cut(s) 59
BsaHI GRCGYC 1 cut(s) 582
BsaI GGTCTC 1 cut(s) 393
BsaJI CCNNGG 3 cut(s) 286, 372, 634
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 260, 293
BseDI CCNNGG 3 cut(s) 286, 372, 634
BseGI GGATG 5 cut(s) 112, 250, 339, 367, 559
BseLI CCNNNNNNNGG 3 cut(s) 232, 260, 293
BseMII CTCAG 2 cut(s) 198, 305
BseRI GAGGAG 2 cut(s) 529, 560
BseXI GCAGC 3 cut(s) 18, 104, 118
BseYI CCCAGC 1 cut(s) 343
BsgI GTGCAG 1 cut(s) 25
Bsh1236I CGCG 1 cut(s) 417
Bsh1285I CGRYCG 1 cut(s) 573
BshFI GGCC 4 cut(s) 119, 211, 302, 633
BsiEI CGRYCG 1 cut(s) 573
BsiHKAI GWGCWC 1 cut(s) 627
BsiHKCI CYCGRG 2 cut(s) 304, 626
BsiSI CCGG 2 cut(s) 212, 288
BslI CCNNNNNNNGG 3 cut(s) 232, 260, 293
BsmAI GTCTC 2 cut(s) 358, 393
BsmI GAATGC 1 cut(s) 63
BsnI GGCC 4 cut(s) 119, 211, 302, 633
Bso31I GGTCTC 1 cut(s) 393
BsoBI CYCGRG 2 cut(s) 304, 626
Bsp1286I GDGCHC 1 cut(s) 627
Bsp143I GATC 3 cut(s) 376, 570, 589
BspACI CCGC 1 cut(s) 653
BspANI GGCC 4 cut(s) 119, 211, 302, 633
BspCNI CTCAG 2 cut(s) 197, 306
BspFNI CGCG 1 cut(s) 417
BspLI GGNNCC 2 cut(s) 120, 303
BspPI GGATC 1 cut(s) 384
BspTNI GGTCTC 1 cut(s) 393
BssECI CCNNGG 3 cut(s) 286, 372, 634
BssMI GATC 3 cut(s) 376, 570, 589
BssNAI GTATAC 1 cut(s) 266
BssNI GRCGYC 1 cut(s) 582
BssSI CACGAG 1 cut(s) 98
BssT1I CCWWGG 2 cut(s) 372, 634
Bst1107I GTATAC 1 cut(s) 266
Bst2BI CACGAG 1 cut(s) 98
Bst4CI ACNGT 1 cut(s) 296
BstACI GRCGYC 1 cut(s) 582
BstAPI GCANNNNNTGC 1 cut(s) 566
BstBAI YACGTR 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 11
BstDEI CTNAG 2 cut(s) 184, 314
BstENI CCTNNNNNAGG 1 cut(s) 258
BstF5I GGATG 5 cut(s) 112, 250, 339, 367, 559
BstFNI CGCG 1 cut(s) 417
BstHHI GCGC 1 cut(s) 487
BstKTI GATC 3 cut(s) 379, 573, 592
BstMAI GTCTC 2 cut(s) 358, 393
BstMBI GATC 3 cut(s) 376, 570, 589
BstMCI CGRYCG 1 cut(s) 573
BstMWI GCNNNNNNNGC 3 cut(s) 423, 443, 566
BstNSI RCATGY 1 cut(s) 13
BstSCI CCNGG 1 cut(s) 286
BstSFI CTRYAG 1 cut(s) 438
BstUI CGCG 1 cut(s) 417
BstV1I GCAGC 3 cut(s) 18, 104, 118
BstZ17I GTATAC 1 cut(s) 266
BsuRI GGCC 4 cut(s) 119, 211, 302, 633
BtsCI GGATG 5 cut(s) 112, 250, 339, 367, 559
Cac8I GCNNGC 1 cut(s) 11
CfoI GCGC 1 cut(s) 487
Cfr13I GGNCC 3 cut(s) 118, 301, 631
CseI GACGC 1 cut(s) 590
Csp6I GTAC 2 cut(s) 221, 340
CviAII CATG 3 cut(s) 10, 350, 578
CviQI GTAC 2 cut(s) 221, 340
DdeI CTNAG 2 cut(s) 184, 314
DpnI GATC 3 cut(s) 378, 572, 591
DpnII GATC 3 cut(s) 376, 570, 589
DrdI GACNNNNNNGTC 1 cut(s) 407
DseDI GACNNNNNNGTC 1 cut(s) 407
Ecl136II GAGCTC 1 cut(s) 625
Eco130I CCWWGG 2 cut(s) 372, 634
Eco24I GRGCYC 1 cut(s) 627
Eco31I GGTCTC 1 cut(s) 393
Eco32I GATATC 1 cut(s) 199
Eco53kI GAGCTC 1 cut(s) 625
Eco72I CACGTG 1 cut(s) 59
Eco88I CYCGRG 2 cut(s) 304, 626
EcoICRI GAGCTC 1 cut(s) 625
EcoNI CCTNNNNNAGG 1 cut(s) 258
EcoO109I RGGNCCY 2 cut(s) 118, 631
EcoRI GAATTC 2 cut(s) 174, 274
EcoRV GATATC 1 cut(s) 199
EcoT14I CCWWGG 2 cut(s) 372, 634
EcoT38I GRGCYC 1 cut(s) 627
ErhI CCWWGG 2 cut(s) 372, 634
FaeI CATG 3 cut(s) 13, 353, 581
FatI CATG 3 cut(s) 9, 349, 577
FauI CCCGC 1 cut(s) 646
FauNDI CATATG 1 cut(s) 394
FblI GTMKAC 2 cut(s) 265, 606
Fnu4HI GCNGC 3 cut(s) 7, 93, 132
FokI GGATG 5 cut(s) 119, 237, 346, 354, 566
FriOI GRGCYC 1 cut(s) 627
Fsp4HI GCNGC 3 cut(s) 7, 93, 132
FspBI CTAG 1 cut(s) 612
GlaI GCGC 1 cut(s) 486
GluI GCNGC 3 cut(s) 7, 93, 132
GsaI CCCAGC 1 cut(s) 347
HaeIII GGCC 4 cut(s) 119, 211, 302, 633
HapII CCGG 2 cut(s) 212, 288
HgaI GACGC 1 cut(s) 590
HhaI GCGC 1 cut(s) 487
Hin1I GRCGYC 1 cut(s) 582
Hin1II CATG 3 cut(s) 13, 353, 581
Hin6I GCGC 1 cut(s) 485
HinP1I GCGC 1 cut(s) 485
HincII GTYRAC 1 cut(s) 607
HindII GTYRAC 1 cut(s) 607
HindIII AAGCTT 1 cut(s) 644
HinfI GANTC 5 cut(s) 125, 149, 189, 236, 316
HpaII CCGG 2 cut(s) 212, 288
HphI GGTGA 1 cut(s) 23
Hpy166II GTNNAC 3 cut(s) 235, 266, 607
Hpy188I TCNGA 3 cut(s) 194, 451, 575
Hpy188III TCNNGA 2 cut(s) 186, 363
Hpy8I GTNNAC 3 cut(s) 235, 266, 607
Hpy99I CGWCG 2 cut(s) 296, 495
HpyAV CCTTC 4 cut(s) 109, 154, 254, 455
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4IV ACGT 1 cut(s) 58
HpyCH4V TGCA 3 cut(s) 6, 13, 353
HpyF10VI GCNNNNNNNGC 3 cut(s) 423, 443, 566
HpyF3I CTNAG 2 cut(s) 184, 314
HpySE526I ACGT 1 cut(s) 58
Hsp92I GRCGYC 1 cut(s) 582
Hsp92II CATG 3 cut(s) 13, 353, 581
HspAI GCGC 1 cut(s) 485
Kzo9I GATC 3 cut(s) 376, 570, 589
LmnI GCTCC 1 cut(s) 214
LpnPI CCDG 8 cut(s) 89, 92, 135, 171, 225, 301, 357, 466
Lsp1109I GCAGC 3 cut(s) 18, 104, 118
LweI GCATC 2 cut(s) 259, 496
MaeI CTAG 1 cut(s) 612
MaeII ACGT 1 cut(s) 58
MaeIII GTNAC 1 cut(s) 638
MalI GATC 3 cut(s) 378, 572, 591
MboI GATC 3 cut(s) 376, 570, 589
MboII GAAGA 2 cut(s) 63, 274
MhlI GDGCHC 1 cut(s) 627
MluCI AATT 4 cut(s) 43, 174, 274, 473
MlyI GAGTC 3 cut(s) 119, 230, 325
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 5 cut(s) 507, 520, 538, 541, 622
MseI TTAA 2 cut(s) 27, 206
MspI CCGG 2 cut(s) 212, 288
MspR9I CCNGG 1 cut(s) 288
Mva1269I GAATGC 1 cut(s) 63
MvnI CGCG 1 cut(s) 417
MwoI GCNNNNNNNGC 3 cut(s) 423, 443, 566
NciI CCSGG 1 cut(s) 288
NdeI CATATG 1 cut(s) 394
NdeII GATC 3 cut(s) 376, 570, 589
NlaIII CATG 3 cut(s) 13, 353, 581
NlaIV GGNNCC 2 cut(s) 120, 303
NmeAIII GCCGAG 1 cut(s) 646
NmuCI GTSAC 1 cut(s) 638
NspI RCATGY 1 cut(s) 13
PaeI GCATGC 1 cut(s) 13
PaeR7I CTCGAG 1 cut(s) 626
PctI GAATGC 1 cut(s) 63
PfeI GAWTC 2 cut(s) 149, 189
PkrI GCNGC 3 cut(s) 8, 94, 133
Ple19I CGATCG 1 cut(s) 573
PleI GAGTC 3 cut(s) 119, 230, 324
PmaCI CACGTG 1 cut(s) 59
PmlI CACGTG 1 cut(s) 59
PpsI GAGTC 3 cut(s) 119, 230, 324
Ppu21I YACGTR 1 cut(s) 59
Psp124BI GAGCTC 1 cut(s) 627
PspCI CACGTG 1 cut(s) 59
PspFI CCCAGC 1 cut(s) 343
PspN4I GGNNCC 2 cut(s) 120, 303
PspPI GGNCC 3 cut(s) 118, 301, 631
PspXI VCTCGAGB 1 cut(s) 626
PvuI CGATCG 1 cut(s) 573
RsaI GTAC 2 cut(s) 222, 341
RsaNI GTAC 2 cut(s) 221, 340
SacI GAGCTC 1 cut(s) 627
SalI GTCGAC 1 cut(s) 605
SaqAI TTAA 2 cut(s) 27, 206
SatI GCNGC 3 cut(s) 7, 93, 132
Sau3AI GATC 3 cut(s) 376, 570, 589
Sau96I GGNCC 3 cut(s) 118, 301, 631
SchI GAGTC 3 cut(s) 119, 230, 325
ScrFI CCNGG 1 cut(s) 288
SduI GDGCHC 1 cut(s) 627
SetI ASST 8 cut(s) 61, 219, 265, 349, 439, 567, 627, 648
SfaNI GCATC 2 cut(s) 259, 496
SfcI CTRYAG 1 cut(s) 438
Sfr274I CTCGAG 1 cut(s) 626
SlaI CTCGAG 1 cut(s) 626
SmlI CTYRAG 1 cut(s) 626
SmoI CTYRAG 1 cut(s) 626
SphI GCATGC 1 cut(s) 13
Sse9I AATT 4 cut(s) 43, 174, 274, 473
SsiI CCGC 1 cut(s) 653
SspI AATATT 1 cut(s) 243
SspMI CTAG 1 cut(s) 612
SstI GAGCTC 1 cut(s) 627
StyD4I CCNGG 1 cut(s) 286
StyI CCWWGG 2 cut(s) 372, 634
TaaI ACNGT 1 cut(s) 296
TaiI ACGT 1 cut(s) 61
TaqI TCGA 4 cut(s) 362, 490, 606, 627
TasI AATT 4 cut(s) 43, 174, 274, 473
TfiI GAWTC 2 cut(s) 149, 189
Tru1I TTAA 2 cut(s) 27, 206
Tru9I TTAA 2 cut(s) 27, 206
TseFI GTSAC 1 cut(s) 638
TseI GCWGC 3 cut(s) 6, 92, 131
Tsp45I GTSAC 1 cut(s) 638
TspDTI ATGAA 2 cut(s) 56, 570
TspGWI ACGGA 1 cut(s) 309
XagI CCTNNNNNAGG 1 cut(s) 258
XapI RAATTY 2 cut(s) 174, 274
XceI RCATGY 1 cut(s) 13
XhoI CTCGAG 1 cut(s) 626
XmiI GTMKAC 2 cut(s) 265, 606
XspI CTAG 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.