Rh3CG199800

Hs1pro-1 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0023050
Physical Location & Seq
Forward (+)
17905453 .. 17905731
279 bp
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UTR
Exon/CDS
Intron
Rh3CG199800.1

Sequence Viewer

Length: 279 bp
ATGAAAACGGTCTACGATAAGGAGTCACTTTTGTTCAGATCAAAGGGACTTGTAGAGATGACCGAGCTTTGCAAGGACTTGAAGAATAATGTTCTGAATGTGCTCGGAGTTGAGGTGGACCAAATGGGTGGGCCAAGGATCCTGGAGGTAGTGATGAGGCTCTACTTGGAGAAAACCGGGTCAGAGAAGATCATCTCCTCTAGGCAATGTATTTTAGCTCTGTATCAAAGGTGGCATCGAATGGCAGCGAGTACTTCAAATTGGGAATCGTCGGGATAA

Protein Analysis

92

Amino Acids

10.52

Weight (kDa)

8.64

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hs1pro-1_C PF07014 2 - 65 1.2e-18 Hs1pro-1 protein C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0023050)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000595.1_g000021
rosa_samantha Rh2BG182400 Rh3CG192600 Rh3CG199800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 12
AclWI GGATC 2 cut(s) 133, 146
AfaI GTAC 1 cut(s) 253
AgsI TTSAA 2 cut(s) 82, 258
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 2 cut(s) 67, 218
AluI AGCT 2 cut(s) 67, 218
Alw21I GWGCWC 1 cut(s) 105
AlwI GGATC 2 cut(s) 133, 146
AoxI GGCC 1 cut(s) 131
ApeKI GCWGC 1 cut(s) 245
AspS9I GGNCC 2 cut(s) 118, 131
AsuC2I CCSGG 1 cut(s) 178
AvaII GGWCC 1 cut(s) 118
BamHI GGATCC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 257
BciT130I CCWGG 1 cut(s) 143
BcnI CCSGG 1 cut(s) 178
BfaI CTAG 1 cut(s) 201
BisI GCNGC 1 cut(s) 246
BlsI GCNGC 1 cut(s) 247
BmcAI AGTACT 1 cut(s) 253
Bme1390I CCNGG 2 cut(s) 143, 178
Bme18I GGWCC 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 118, 131
BmiI GGNNCC 1 cut(s) 140
BmrFI CCNGG 2 cut(s) 143, 178
BmsI GCATC 1 cut(s) 244
BpmI CTGGAG 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 178
BsaJI CCNNGG 1 cut(s) 134
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bse3DI GCAATG 1 cut(s) 212
BseBI CCWGG 1 cut(s) 143
BseDI CCNNGG 1 cut(s) 134
BseMI GCAATG 1 cut(s) 212
BseRI GAGGAG 1 cut(s) 187
BseXI GCAGC 1 cut(s) 257
BshFI GGCC 1 cut(s) 133
BsiHKAI GWGCWC 1 cut(s) 105
BsiSI CCGG 1 cut(s) 177
BslFI GGGAC 1 cut(s) 60
BsmFI GGGAC 1 cut(s) 60
BsnI GGCC 1 cut(s) 133
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 3 cut(s) 38, 138, 189
BspANI GGCC 1 cut(s) 133
BspLI GGNNCC 1 cut(s) 140
BspPI GGATC 2 cut(s) 133, 146
BsrDI GCAATG 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 134
BssMI GATC 3 cut(s) 38, 138, 189
BssT1I CCWWGG 1 cut(s) 134
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 1 cut(s) 10
BstKTI GATC 3 cut(s) 41, 141, 192
BstMBI GATC 3 cut(s) 38, 138, 189
BstNI CCWGG 1 cut(s) 143
BstSCI CCNGG 2 cut(s) 141, 176
BstV1I GCAGC 1 cut(s) 257
BstX2I RGATCY 1 cut(s) 138
BstXI CCANNNNNNTGG 1 cut(s) 128
BstYI RGATCY 1 cut(s) 138
BsuRI GGCC 1 cut(s) 133
Cfr13I GGNCC 2 cut(s) 118, 131
Csp6I GTAC 1 cut(s) 252
CviJI RGCY 4 cut(s) 67, 133, 160, 218
CviKI_1 RGCY 4 cut(s) 67, 133, 160, 218
CviQI GTAC 1 cut(s) 252
DpnI GATC 3 cut(s) 40, 140, 191
DpnII GATC 3 cut(s) 38, 138, 189
Eco130I CCWWGG 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 118
EcoRII CCWGG 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 134
ErhI CCWWGG 1 cut(s) 134
FaqI GGGAC 1 cut(s) 60
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 1 cut(s) 246
Fsp4HI GCNGC 1 cut(s) 246
FspBI CTAG 1 cut(s) 201
GluI GCNGC 1 cut(s) 246
GsuI CTGGAG 1 cut(s) 164
HaeIII GGCC 1 cut(s) 133
HapII CCGG 1 cut(s) 177
HinfI GANTC 2 cut(s) 23, 266
HpaII CCGG 1 cut(s) 177
Hpy166II GTNNAC 2 cut(s) 13, 118
Hpy188I TCNGA 4 cut(s) 38, 96, 107, 184
Hpy188III TCNNGA 1 cut(s) 273
Hpy8I GTNNAC 2 cut(s) 13, 118
Hpy99I CGWCG 1 cut(s) 274
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4V TGCA 1 cut(s) 72
Kzo9I GATC 3 cut(s) 38, 138, 189
LpnPI CCDG 3 cut(s) 128, 155, 190
Lsp1109I GCAGC 1 cut(s) 257
LweI GCATC 1 cut(s) 244
MaeI CTAG 1 cut(s) 201
MaeIII GTNAC 1 cut(s) 24
MalI GATC 3 cut(s) 40, 140, 191
MboI GATC 3 cut(s) 38, 138, 189
MboII GAAGA 2 cut(s) 94, 199
MflI RGATCY 1 cut(s) 138
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 1 cut(s) 259
MlyI GAGTC 1 cut(s) 32
MnlI CCTC 4 cut(s) 106, 139, 150, 208
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 2 cut(s) 143, 178
MvaI CCWGG 1 cut(s) 143
NciI CCSGG 1 cut(s) 178
NdeII GATC 3 cut(s) 38, 138, 189
NlaIV GGNNCC 1 cut(s) 140
NmuCI GTSAC 1 cut(s) 24
PfeI GAWTC 1 cut(s) 266
PfoI TCCNGGA 1 cut(s) 141
PkrI GCNGC 1 cut(s) 247
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 1 cut(s) 140
PspPI GGNCC 2 cut(s) 118, 131
PsuI RGATCY 1 cut(s) 138
RsaI GTAC 1 cut(s) 253
RsaNI GTAC 1 cut(s) 252
SatI GCNGC 1 cut(s) 246
Sau3AI GATC 3 cut(s) 38, 138, 189
Sau96I GGNCC 2 cut(s) 118, 131
ScaI AGTACT 1 cut(s) 253
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 2 cut(s) 143, 178
SduI GDGCHC 1 cut(s) 105
SetI ASST 5 cut(s) 69, 117, 150, 220, 233
SfaNI GCATC 1 cut(s) 244
SinI GGWCC 1 cut(s) 118
Sse9I AATT 1 cut(s) 259
SspMI CTAG 1 cut(s) 201
StyD4I CCNGG 2 cut(s) 141, 176
StyI CCWWGG 1 cut(s) 134
TaaI ACNGT 1 cut(s) 10
TaqI TCGA 1 cut(s) 238
TaqII GACCGA 1 cut(s) 77
TasI AATT 1 cut(s) 259
TatI WGTACW 1 cut(s) 251
TfiI GAWTC 1 cut(s) 266
TseFI GTSAC 1 cut(s) 24
TseI GCWGC 1 cut(s) 245
Tsp45I GTSAC 1 cut(s) 24
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 12
XspI CTAG 1 cut(s) 201
ZrmI AGTACT 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.