Rh3DG261500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0026027
Physical Location & Seq
Reverse (-)
25948274 .. 25948753
480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG261500.1

Sequence Viewer

Length: 480 bp
ATGGTTGTGGATGTTGCTGCAGCTGCTGTAGGACTAGCATTATCAGTGCTGTATAAGGGCATTGAGGAACTTATCAGAAAGTGCGTGATCGATAAGGTTATGGAGTTTAATCCCCTCCTCGGGAAAATCAATATGAAGATAAAGGAATTGGAGCCAGAGATAGAAGAGATCGAGAAAAGGAATCAAGAATTGGATCGCCCAGAGGAATTGAAAGATTTCACAAAAGTGATCGAGGATGGCACAGCGCTTGTTGACAAGTTAAAGGAGGTTACCCATTGGTACAAGAAACCTAAGTACACCAACAAACTCAAGCAACTCGATGAATCTCTGGATACACAGATAATGTTACTGCTGGTGCGGTCAGCAAGTAAGGTCGCTAACCTTTCGGTTATAGCAAGTATTACCGAGGGGAACACCACAAATATCCGTATGCAAATGGGGAATATGAGTGTGCAAATGGGAGTACGAGTATGCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.99

Weight (kDa)

6.62

Isoelectric Point (pI)

32.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 5 - 112 3.5e-17 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026027)

Species Orthologous Gene IDs
rosa_samantha Rh3AG235800 Rh3DG261500 Rh4DG163200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 358
AclWI GGATC 1 cut(s) 201
AfaI GTAC 3 cut(s) 281, 296, 465
AfeI AGCGCT 1 cut(s) 246
AfiI CCNNNNNNNGG 2 cut(s) 119, 120
AgsI TTSAA 1 cut(s) 211
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AleI CACNNNNGTG 1 cut(s) 224
AluBI AGCT 1 cut(s) 23
AluI AGCT 1 cut(s) 23
AlwI GGATC 1 cut(s) 201
AlwNI CAGNNNCTG 1 cut(s) 26
Ama87I CYCGRG 1 cut(s) 119
Aor51HI AGCGCT 1 cut(s) 246
ApeKI GCWGC 3 cut(s) 17, 20, 23
Asp700I GAANNNNTTC 1 cut(s) 215
AspLEI GCGC 1 cut(s) 247
AvaI CYCGRG 1 cut(s) 119
BbvI GCAGC 3 cut(s) 4, 10, 32
BccI CCATC 1 cut(s) 230
BciVI GTATCC 1 cut(s) 325
BfaI CTAG 1 cut(s) 35
BfmI CTRYAG 2 cut(s) 18, 27
BfoI RGCGCY 1 cut(s) 248
BfuI GTATCC 1 cut(s) 325
BisI GCNGC 3 cut(s) 18, 21, 24
BlsI GCNGC 3 cut(s) 19, 22, 25
BmeT110I CYCGRG 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 293
Bsa29I ATCGAT 1 cut(s) 90
BsaJI CCNNGG 2 cut(s) 118, 405
BsaXI ACNNNNNCTCC 1 cut(s) 453
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 120
BseCI ATCGAT 1 cut(s) 90
BseDI CCNNGG 2 cut(s) 118, 405
BseGI GGATG 2 cut(s) 16, 241
BseLI CCNNNNNNNGG 2 cut(s) 119, 120
BseRI GAGGAG 1 cut(s) 107
BseXI GCAGC 3 cut(s) 4, 10, 32
BshVI ATCGAT 1 cut(s) 90
BsiHKCI CYCGRG 1 cut(s) 119
BslI CCNNNNNNNGG 2 cut(s) 119, 120
BsoBI CYCGRG 1 cut(s) 119
Bsp143I GATC 4 cut(s) 87, 168, 193, 228
BspACI CCGC 1 cut(s) 358
BspDI ATCGAT 1 cut(s) 90
BspLI GGNNCC 1 cut(s) 153
BspMAI CTGCAG 1 cut(s) 22
BspPI GGATC 1 cut(s) 201
BssECI CCNNGG 2 cut(s) 118, 405
BssMI GATC 4 cut(s) 87, 168, 193, 228
Bst6I CTCTTC 1 cut(s) 159
BstDEI CTNAG 1 cut(s) 291
BstEII GGTNACC 1 cut(s) 268
BstF5I GGATG 2 cut(s) 16, 241
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 4 cut(s) 90, 171, 196, 231
BstMBI GATC 4 cut(s) 87, 168, 193, 228
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstPI GGTNACC 1 cut(s) 268
BstSFI CTRYAG 2 cut(s) 18, 27
BstV1I GCAGC 3 cut(s) 4, 10, 32
Bsu15I ATCGAT 1 cut(s) 90
BsuI GTATCC 1 cut(s) 325
BsuTUI ATCGAT 1 cut(s) 90
BtsCI GGATG 2 cut(s) 16, 241
BtsIMutI CAGTG 1 cut(s) 51
CaiI CAGNNNCTG 1 cut(s) 26
CfoI GCGC 1 cut(s) 247
ClaI ATCGAT 1 cut(s) 90
Csp6I GTAC 3 cut(s) 280, 295, 464
CviJI RGCY 2 cut(s) 23, 154
CviKI_1 RGCY 2 cut(s) 23, 154
CviQI GTAC 3 cut(s) 280, 295, 464
DdeI CTNAG 1 cut(s) 291
DpnI GATC 4 cut(s) 89, 170, 195, 230
DpnII GATC 4 cut(s) 87, 168, 193, 228
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco47III AGCGCT 1 cut(s) 246
Eco88I CYCGRG 1 cut(s) 119
Eco91I GGTNACC 1 cut(s) 268
EcoO65I GGTNACC 1 cut(s) 268
FaiI YATR 7 cut(s) 54, 101, 134, 392, 431, 446, 472
Fnu4HI GCNGC 3 cut(s) 18, 21, 24
FokI GGATG 2 cut(s) 23, 248
Fsp4HI GCNGC 3 cut(s) 18, 21, 24
FspBI CTAG 1 cut(s) 35
GlaI GCGC 1 cut(s) 246
GluI GCNGC 3 cut(s) 18, 21, 24
HaeII RGCGCY 1 cut(s) 248
HhaI GCGC 1 cut(s) 247
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 2 cut(s) 181, 323
Hpy166II GTNNAC 2 cut(s) 253, 297
Hpy188I TCNGA 1 cut(s) 77
Hpy188III TCNNGA 4 cut(s) 121, 172, 185, 329
Hpy8I GTNNAC 2 cut(s) 253, 297
HpyCH4V TGCA 4 cut(s) 20, 433, 454, 474
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 1 cut(s) 291
HspAI GCGC 1 cut(s) 245
Kzo9I GATC 4 cut(s) 87, 168, 193, 228
LmnI GCTCC 1 cut(s) 151
LpnPI CCDG 4 cut(s) 168, 213, 314, 338
Lsp1109I GCAGC 3 cut(s) 4, 10, 32
MaeI CTAG 1 cut(s) 35
MaeIII GTNAC 2 cut(s) 268, 345
MalI GATC 4 cut(s) 89, 170, 195, 230
MboI GATC 4 cut(s) 87, 168, 193, 228
MboII GAAGA 2 cut(s) 148, 176
MluCI AATT 3 cut(s) 146, 188, 206
MnlI CCTC 7 cut(s) 58, 125, 128, 196, 226, 259, 400
MroXI GAANNNNTTC 1 cut(s) 215
MseI TTAA 2 cut(s) 108, 260
MslI CAYNNNNRTG 1 cut(s) 224
MspA1I CMGCKG 1 cut(s) 23
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeII GATC 4 cut(s) 87, 168, 193, 228
NlaIV GGNNCC 1 cut(s) 153
OliI CACNNNNGTG 1 cut(s) 224
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 2 cut(s) 181, 323
PkrI GCNGC 3 cut(s) 19, 22, 25
PspEI GGTNACC 1 cut(s) 268
PspN4I GGNNCC 1 cut(s) 153
PstI CTGCAG 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 26
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 3 cut(s) 281, 296, 465
RsaNI GTAC 3 cut(s) 280, 295, 464
RseI CAYNNNNRTG 1 cut(s) 224
SaqAI TTAA 2 cut(s) 108, 260
SatI GCNGC 3 cut(s) 18, 21, 24
Sau3AI GATC 4 cut(s) 87, 168, 193, 228
SetI ASST 6 cut(s) 25, 99, 270, 292, 375, 384
SfcI CTRYAG 2 cut(s) 18, 27
SmiMI CAYNNNNRTG 1 cut(s) 224
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 3 cut(s) 146, 188, 206
SsiI CCGC 1 cut(s) 358
SspMI CTAG 1 cut(s) 35
TaqI TCGA 4 cut(s) 90, 171, 231, 318
TasI AATT 3 cut(s) 146, 188, 206
TatI WGTACW 1 cut(s) 294
TfiI GAWTC 2 cut(s) 181, 323
Tru1I TTAA 2 cut(s) 108, 260
Tru9I TTAA 2 cut(s) 108, 260
TscAI CASTG 1 cut(s) 51
TseI GCWGC 3 cut(s) 17, 20, 23
TspDTI ATGAA 2 cut(s) 149, 336
TspGWI ACGGA 1 cut(s) 416
TspRI CASTG 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.