Rh4DG163200

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0026027
Physical Location & Seq
Reverse (-)
32421098 .. 32421910
813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG163200.1

Sequence Viewer

Length: 813 bp
ATGGTTGTAGATGTTGCCGCATCTGCTGTAGGAGTAGCATTATCGCTGCTGTGTAAGGCCATTGAAGAAGTTATCAGAAAGTGCGTGATCGACAAGATTGTGCAGTTCAATCCCCTGCTCGAGAAGATCAATACCAAGCTGAAGACAGTGAAGCCAGGGATAGAAGAGATCGAGAAAAGCAATCTAGAATTGGATCGCACAGGTGAATTGACAGATTTCAAACAAGTAATCGAGGATATCACAGCGCTTGTTCTTAAGTTACAAGAGGTTCCCCATTGGTACAAGAAACCTAAATACACCGACAAACTCAAAGAACTCGATGAATCTCTGGATACACAGATAATGTTACTGCAGGTGCCGACAGCAAGTAAGGTCTCTAACCTTTCGGTTATAACAAGTATTATCGAGGGGGACACCAGAAATATCCGTATGCAAATGGGGAATATGAGTGTGCAAGTGGGGAGTATGAGTATGCAAATGGAGAATATGGGTATGCAAATAGAGGATTTGAATACGAACCTTGAGATGATCAGGGATATGCGACAGAATGGCCTAGTGCAAAATCAAGTTAAAGGTTGTTGGTCTGGAGTACCTGAACCGCCATCATTTACAGTTGGATTGGATTTACGTTTGGATGAATTGAAGAAGAAACTTCTTGAGGATGGGGTGTCAATGGGTCTGGTCACTGGTCATCCAGGCTCTGGGAAAACCACTTTGGCAAAAGCTCTTTGTCAAGATAAGAAAGTGAAGGGTATATTTGATATCTGTTTCTTTTTATCTTCTAAATGGAAAAGTTTTAGAGTTTCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

270

Amino Acids

30.15

Weight (kDa)

6.76

Isoelectric Point (pI)

25.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 7 - 112 7.7e-14 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 211 - 259 3.8e-06 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026027)

Species Orthologous Gene IDs
rosa_samantha Rh3AG235800 Rh3DG261500 Rh4DG163200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 392
AarI CACCTGC 1 cut(s) 343
Acc36I ACCTGC 1 cut(s) 343
AccB1I GGYRCC 1 cut(s) 355
AccB7I CCANNNNNTGG 1 cut(s) 701
AciI CCGC 2 cut(s) 18, 599
AclWI GGATC 1 cut(s) 201
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 2 cut(s) 281, 591
AfeI AGCGCT 1 cut(s) 246
AfiI CCNNNNNNNGG 1 cut(s) 701
AflII CTTAAG 1 cut(s) 254
AgsI TTSAA 5 cut(s) 65, 109, 220, 511, 643
AjnI CCWGG 2 cut(s) 154, 694
AjuI GAANNNNNNNTTGG 2 cut(s) 698, 730
AluBI AGCT 2 cut(s) 139, 725
AluI AGCT 2 cut(s) 139, 725
Alw26I GTCTC 1 cut(s) 379
AlwI GGATC 1 cut(s) 201
AlwNI CAGNNNCTG 1 cut(s) 701
Ama87I CYCGRG 1 cut(s) 119
Aor51HI AGCGCT 1 cut(s) 246
AoxI GGCC 2 cut(s) 57, 550
ApeKI GCWGC 1 cut(s) 46
AspLEI GCGC 1 cut(s) 247
AsuHPI GGTGA 1 cut(s) 215
AvaI CYCGRG 1 cut(s) 119
BanI GGYRCC 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 149
BbvI GCAGC 1 cut(s) 33
BccI CCATC 2 cut(s) 610, 656
BciT130I CCWGG 2 cut(s) 156, 696
BciVI GTATCC 1 cut(s) 325
BclI TGATCA 1 cut(s) 528
BcoDI GTCTC 1 cut(s) 379
BfaI CTAG 2 cut(s) 185, 554
BfmI CTRYAG 2 cut(s) 27, 350
BfoI RGCGCY 1 cut(s) 248
BfrI CTTAAG 1 cut(s) 254
BfuAI ACCTGC 1 cut(s) 343
BfuI GTATCC 1 cut(s) 325
BisI GCNGC 2 cut(s) 18, 47
BlsI GCNGC 2 cut(s) 19, 48
Bme1390I CCNGG 2 cut(s) 156, 696
BmeT110I CYCGRG 1 cut(s) 119
BmiI GGNNCC 2 cut(s) 270, 357
BmrFI CCNGG 2 cut(s) 156, 696
BmsI GCATC 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 149
BpmI CTGGAG 1 cut(s) 606
BpuEI CTTGAG 2 cut(s) 542, 677
BsaI GGTCTC 1 cut(s) 379
BsaJI CCNNGG 1 cut(s) 155
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
Bsc4I CCNNNNNNNGG 1 cut(s) 701
Bse1I ACTGG 1 cut(s) 691
BseBI CCWGG 2 cut(s) 156, 696
BseDI CCNNGG 1 cut(s) 155
BseGI GGATG 3 cut(s) 640, 667, 691
BseLI CCNNNNNNNGG 1 cut(s) 701
BseNI ACTGG 1 cut(s) 691
BseXI GCAGC 1 cut(s) 33
BsgI GTGCAG 1 cut(s) 122
BshFI GGCC 2 cut(s) 59, 552
BshNI GGYRCC 1 cut(s) 355
BsiHKCI CYCGRG 1 cut(s) 119
BslFI GGGAC 1 cut(s) 425
BslI CCNNNNNNNGG 1 cut(s) 701
BsmAI GTCTC 1 cut(s) 379
BsmFI GGGAC 1 cut(s) 425
BsnI GGCC 2 cut(s) 59, 552
Bso31I GGTCTC 1 cut(s) 379
BsoBI CYCGRG 1 cut(s) 119
Bsp143I GATC 5 cut(s) 87, 126, 168, 193, 528
BspACI CCGC 2 cut(s) 18, 599
BspANI GGCC 2 cut(s) 59, 552
BspLI GGNNCC 2 cut(s) 270, 357
BspMAI CTGCAG 1 cut(s) 354
BspMI ACCTGC 1 cut(s) 343
BspPI GGATC 1 cut(s) 201
BspT107I GGYRCC 1 cut(s) 355
BspTI CTTAAG 1 cut(s) 254
BspTNI GGTCTC 1 cut(s) 379
BsrI ACTGG 1 cut(s) 691
BssECI CCNNGG 1 cut(s) 155
BssMI GATC 5 cut(s) 87, 126, 168, 193, 528
Bst2UI CCWGG 2 cut(s) 156, 696
Bst4CI ACNGT 2 cut(s) 148, 613
Bst6I CTCTTC 1 cut(s) 159
BstAFI CTTAAG 1 cut(s) 254
BstF5I GGATG 3 cut(s) 640, 667, 691
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 5 cut(s) 90, 129, 171, 196, 531
BstMAI GTCTC 1 cut(s) 379
BstMBI GATC 5 cut(s) 87, 126, 168, 193, 528
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNI CCWGG 2 cut(s) 156, 696
BstSCI CCNGG 2 cut(s) 154, 694
BstSFI CTRYAG 2 cut(s) 27, 350
BstV1I GCAGC 1 cut(s) 33
BstV2I GAAGAC 1 cut(s) 149
BsuI GTATCC 1 cut(s) 325
BsuRI GGCC 2 cut(s) 59, 552
BtsCI GGATG 3 cut(s) 640, 667, 691
BtsIMutI CAGTG 2 cut(s) 153, 684
BveI ACCTGC 1 cut(s) 343
CaiI CAGNNNCTG 1 cut(s) 701
CfoI GCGC 1 cut(s) 247
Csp6I GTAC 2 cut(s) 280, 590
CviJI RGCY 6 cut(s) 59, 139, 154, 552, 699, 725
CviKI_1 RGCY 6 cut(s) 59, 139, 154, 552, 699, 725
CviQI GTAC 2 cut(s) 280, 590
DpnI GATC 5 cut(s) 89, 128, 170, 195, 530
DpnII GATC 5 cut(s) 87, 126, 168, 193, 528
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 379
Eco32I GATATC 2 cut(s) 238, 763
Eco47III AGCGCT 1 cut(s) 246
Eco57I CTGAAG 1 cut(s) 161
Eco88I CYCGRG 1 cut(s) 119
EcoRII CCWGG 2 cut(s) 154, 694
EcoRV GATATC 2 cut(s) 238, 763
FaiI YATR 9 cut(s) 392, 431, 446, 467, 473, 488, 494, 539, 755
FaqI GGGAC 1 cut(s) 425
FbaI TGATCA 1 cut(s) 528
Fnu4HI GCNGC 2 cut(s) 18, 47
FokI GGATG 3 cut(s) 647, 674, 678
Fsp4HI GCNGC 2 cut(s) 18, 47
FspBI CTAG 2 cut(s) 185, 554
GlaI GCGC 1 cut(s) 246
GluI GCNGC 2 cut(s) 18, 47
GsuI CTGGAG 1 cut(s) 606
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 2 cut(s) 59, 552
HhaI GCGC 1 cut(s) 247
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HinfI GANTC 1 cut(s) 323
HphI GGTGA 1 cut(s) 215
Hpy188I TCNGA 1 cut(s) 77
Hpy188III TCNNGA 7 cut(s) 121, 172, 185, 329, 585, 656, 734
HpyAV CCTTC 1 cut(s) 742
HpyCH4III ACNGT 2 cut(s) 148, 613
HpyCH4IV ACGT 1 cut(s) 628
HpyCH4V TGCA 7 cut(s) 103, 352, 433, 454, 475, 496, 559
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpySE526I ACGT 1 cut(s) 628
HspAI GCGC 1 cut(s) 245
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 5 cut(s) 87, 126, 168, 193, 528
Lsp1109I GCAGC 1 cut(s) 33
LweI GCATC 1 cut(s) 29
MaeI CTAG 2 cut(s) 185, 554
MaeII ACGT 1 cut(s) 628
MaeIII GTNAC 3 cut(s) 258, 345, 682
MalI GATC 5 cut(s) 89, 128, 170, 195, 530
MboI GATC 5 cut(s) 87, 126, 168, 193, 528
MboII GAAGA 7 cut(s) 77, 136, 154, 176, 655, 658, 771
MluCI AATT 4 cut(s) 188, 206, 638, 808
MmeI TCCRAC 1 cut(s) 595
MnlI CCTC 5 cut(s) 226, 259, 400, 496, 652
MseI TTAA 2 cut(s) 255, 570
MspCI CTTAAG 1 cut(s) 254
MspR9I CCNGG 2 cut(s) 156, 696
MvaI CCWGG 2 cut(s) 156, 696
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeII GATC 5 cut(s) 87, 126, 168, 193, 528
NlaIV GGNNCC 2 cut(s) 270, 357
NmuCI GTSAC 1 cut(s) 682
PaeR7I CTCGAG 1 cut(s) 119
PaqCI CACCTGC 1 cut(s) 343
PfeI GAWTC 1 cut(s) 323
PflMI CCANNNNNTGG 1 cut(s) 701
PkrI GCNGC 2 cut(s) 19, 48
PsiI TTATAA 1 cut(s) 392
Psp6I CCWGG 2 cut(s) 154, 694
PspGI CCWGG 2 cut(s) 154, 694
PspN4I GGNNCC 2 cut(s) 270, 357
PstI CTGCAG 1 cut(s) 354
PstNI CAGNNNCTG 1 cut(s) 701
RsaI GTAC 2 cut(s) 281, 591
RsaNI GTAC 2 cut(s) 280, 590
SaqAI TTAA 2 cut(s) 255, 570
SatI GCNGC 2 cut(s) 18, 47
Sau3AI GATC 5 cut(s) 87, 126, 168, 193, 528
ScrFI CCNGG 2 cut(s) 156, 696
SfaNI GCATC 1 cut(s) 29
SfcI CTRYAG 2 cut(s) 27, 350
Sfr274I CTCGAG 1 cut(s) 119
SlaI CTCGAG 1 cut(s) 119
SmlI CTYRAG 4 cut(s) 119, 254, 521, 656
SmoI CTYRAG 4 cut(s) 119, 254, 521, 656
Sse9I AATT 4 cut(s) 188, 206, 638, 808
SsiI CCGC 2 cut(s) 18, 599
SspMI CTAG 2 cut(s) 185, 554
StyD4I CCNGG 2 cut(s) 154, 694
TaaI ACNGT 2 cut(s) 148, 613
TaiI ACGT 1 cut(s) 631
TaqI TCGA 6 cut(s) 90, 120, 171, 231, 318, 405
TasI AATT 4 cut(s) 188, 206, 638, 808
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 1 cut(s) 323
Tru1I TTAA 2 cut(s) 255, 570
Tru9I TTAA 2 cut(s) 255, 570
TscAI CASTG 2 cut(s) 153, 691
TseFI GTSAC 1 cut(s) 682
TseI GCWGC 1 cut(s) 46
Tsp45I GTSAC 1 cut(s) 682
TspDTI ATGAA 2 cut(s) 336, 651
TspGWI ACGGA 1 cut(s) 416
TspRI CASTG 2 cut(s) 153, 691
Van91I CCANNNNNTGG 1 cut(s) 701
Vha464I CTTAAG 1 cut(s) 254
XbaI TCTAGA 1 cut(s) 184
XhoI CTCGAG 1 cut(s) 119
XspI CTAG 2 cut(s) 185, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.