Rh4AG103400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0025850
Physical Location & Seq
Reverse (-)
22057397 .. 22060072
2676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG103400.1

Sequence Viewer

Length: 372 bp
ATGTCTGGTAACTTGAGTGGGAAGGTCATGGAGCTCATGACAAGTCAAGAGGGAACAGAAGGAATTGGATATTGGTTTTGGAAGATGGTCTCTGCAACTTTGGAGCCTCAGCCTCACCAGCTTATGATCAGATCAACTGCATCCACTGGTGACTGTCCAGGTGGCCCTTCTTCAGTTTATGGTGGTGATTTCGACATCACCAGTACGACCATGGACTGCAACCTGAGCTTTCTTCAAGATATCCACAACTATGGAGAAGTTTCCAGAATTGTCTCATCATCATCATGGAGGACTATGAAGCAGAAAATTGTGGCCAAGGAGGAAACTGATGTACAAGAAGAAAGAGGAATATGGGGCGCCGGATCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

123

Amino Acids

13.48

Weight (kDa)

4.74

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025850)

Species Orthologous Gene IDs
rosa_roxburghii Rroxscaffold_5G00345590
rosa_samantha Rh4AG103400 Rh4CG111700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 356
AclWI GGATC 1 cut(s) 370
AcoI YGGCCR 1 cut(s) 312
AcuI CTGAAG 1 cut(s) 156
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 2 cut(s) 205, 333
AgsI TTSAA 1 cut(s) 236
AjnI CCWGG 1 cut(s) 157
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AluBI AGCT 3 cut(s) 34, 121, 228
AluI AGCT 3 cut(s) 34, 121, 228
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 2 cut(s) 94, 277
AlwI GGATC 1 cut(s) 370
AoxI GGCC 2 cut(s) 163, 312
AspLEI GCGC 1 cut(s) 359
AspS9I GGNCC 1 cut(s) 164
AsuHPI GGTGA 4 cut(s) 107, 161, 190, 197
BalI TGGCCA 1 cut(s) 314
BanI GGYRCC 1 cut(s) 356
BanII GRGCYC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 36
BbvCI CCTCAGC 1 cut(s) 108
BccI CCATC 1 cut(s) 79
BciT130I CCWGG 1 cut(s) 159
BclI TGATCA 1 cut(s) 126
BcoDI GTCTC 2 cut(s) 94, 277
BfoI RGCGCY 1 cut(s) 360
Bme1390I CCNGG 1 cut(s) 159
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 2 cut(s) 105, 358
BmrFI CCNGG 1 cut(s) 159
BmsI GCATC 1 cut(s) 149
Bpu10I CCTNAGC 2 cut(s) 108, 224
BpuEI CTTGAG 1 cut(s) 34
BsaHI GRCGYC 1 cut(s) 357
BsaI GGTCTC 1 cut(s) 94
BsaJI CCNNGG 2 cut(s) 210, 315
Bse1I ACTGG 2 cut(s) 151, 201
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 2 cut(s) 210, 315
BseGI GGATG 1 cut(s) 140
BseMII CTCAG 2 cut(s) 122, 215
BseNI ACTGG 2 cut(s) 151, 201
BshFI GGCC 2 cut(s) 165, 314
BshNI GGYRCC 1 cut(s) 356
BsiHKAI GWGCWC 1 cut(s) 36
BsiSI CCGG 1 cut(s) 360
BsmAI GTCTC 2 cut(s) 94, 277
BsnI GGCC 2 cut(s) 165, 314
Bso31I GGTCTC 1 cut(s) 94
Bsp1286I GDGCHC 1 cut(s) 36
Bsp1407I TGTACA 1 cut(s) 331
Bsp143I GATC 3 cut(s) 126, 131, 362
Bsp19I CCATGG 1 cut(s) 210
BspANI GGCC 2 cut(s) 165, 314
BspCNI CTCAG 2 cut(s) 121, 216
BspHI TCATGA 1 cut(s) 36
BspLI GGNNCC 2 cut(s) 105, 358
BspPI GGATC 1 cut(s) 370
BspT107I GGYRCC 1 cut(s) 356
BspTNI GGTCTC 1 cut(s) 94
BsrGI TGTACA 1 cut(s) 331
BsrI ACTGG 2 cut(s) 151, 201
BssECI CCNNGG 2 cut(s) 210, 315
BssMI GATC 3 cut(s) 126, 131, 362
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 2 cut(s) 210, 315
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 1 cut(s) 155
BstACI GRCGYC 1 cut(s) 357
BstAUI TGTACA 1 cut(s) 331
BstDEI CTNAG 2 cut(s) 108, 224
BstDSI CCRYGG 1 cut(s) 210
BstF5I GGATG 1 cut(s) 140
BstH2I RGCGCY 1 cut(s) 360
BstHHI GCGC 1 cut(s) 359
BstKTI GATC 3 cut(s) 129, 134, 365
BstMAI GTCTC 2 cut(s) 94, 277
BstMBI GATC 3 cut(s) 126, 131, 362
BstMWI GCNNNNNNNGC 2 cut(s) 118, 225
BstNI CCWGG 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 157
BstXI CCANNNNNNTGG 1 cut(s) 251
BsuRI GGCC 2 cut(s) 165, 314
BtgI CCRYGG 1 cut(s) 210
BtsCI GGATG 1 cut(s) 140
BtsIMutI CAGTG 1 cut(s) 144
CciI TCATGA 1 cut(s) 36
CfoI GCGC 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 164
Csp6I GTAC 2 cut(s) 204, 332
CviAII CATG 4 cut(s) 28, 37, 211, 285
CviJI RGCY 7 cut(s) 34, 106, 112, 121, 165, 228, 314
CviKI_1 RGCY 7 cut(s) 34, 106, 112, 121, 165, 228, 314
CviQI GTAC 2 cut(s) 204, 332
DdeI CTNAG 2 cut(s) 108, 224
DinI GGCGCC 1 cut(s) 358
DpnI GATC 3 cut(s) 128, 133, 364
DpnII GATC 3 cut(s) 126, 131, 362
EaeI YGGCCR 1 cut(s) 312
Ecl136II GAGCTC 1 cut(s) 34
Eco130I CCWWGG 2 cut(s) 210, 315
Eco24I GRGCYC 1 cut(s) 36
Eco31I GGTCTC 1 cut(s) 94
Eco32I GATATC 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 156
EcoICRI GAGCTC 1 cut(s) 34
EcoRII CCWGG 1 cut(s) 157
EcoRV GATATC 1 cut(s) 241
EcoT14I CCWWGG 2 cut(s) 210, 315
EcoT38I GRGCYC 1 cut(s) 36
EgeI GGCGCC 1 cut(s) 358
EheI GGCGCC 1 cut(s) 358
ErhI CCWWGG 2 cut(s) 210, 315
FaeI CATG 4 cut(s) 31, 40, 214, 288
FaiI YATR 9 cut(s) 29, 38, 125, 180, 212, 252, 286, 296, 352
FatI CATG 4 cut(s) 27, 36, 210, 284
FbaI TGATCA 1 cut(s) 126
FokI GGATG 1 cut(s) 127
FriOI GRGCYC 1 cut(s) 36
GlaI GCGC 1 cut(s) 358
HaeII RGCGCY 1 cut(s) 360
HaeIII GGCC 2 cut(s) 165, 314
HapII CCGG 1 cut(s) 360
HhaI GCGC 1 cut(s) 359
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 4 cut(s) 31, 40, 214, 288
Hin6I GCGC 1 cut(s) 357
HinP1I GCGC 1 cut(s) 357
HpaII CCGG 1 cut(s) 360
HphI GGTGA 4 cut(s) 107, 161, 190, 197
Hpy188I TCNGA 1 cut(s) 131
Hpy188III TCNNGA 4 cut(s) 37, 47, 236, 264
HpyAV CCTTC 3 cut(s) 16, 53, 177
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4V TGCA 3 cut(s) 95, 140, 219
HpyF10VI GCNNNNNNNGC 2 cut(s) 118, 225
HpyF3I CTNAG 2 cut(s) 108, 224
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 4 cut(s) 31, 40, 214, 288
HspAI GCGC 1 cut(s) 357
KasI GGCGCC 1 cut(s) 356
Ksp22I TGATCA 1 cut(s) 126
Kzo9I GATC 3 cut(s) 126, 131, 362
LmnI GCTCC 2 cut(s) 31, 103
LpnPI CCDG 7 cut(s) 131, 132, 144, 171, 214, 236, 277
LweI GCATC 1 cut(s) 149
MaeIII GTNAC 2 cut(s) 8, 149
MalI GATC 3 cut(s) 128, 133, 364
MboI GATC 3 cut(s) 126, 131, 362
MboII GAAGA 4 cut(s) 94, 162, 224, 350
MhlI GDGCHC 1 cut(s) 36
MlsI TGGCCA 1 cut(s) 314
MluCI AATT 4 cut(s) 63, 267, 306, 367
MluNI TGGCCA 1 cut(s) 314
Mly113I GGCGCC 1 cut(s) 357
MnlI CCTC 6 cut(s) 43, 117, 123, 282, 313, 338
Mox20I TGGCCA 1 cut(s) 314
MscI TGGCCA 1 cut(s) 314
MseI TTAA 1 cut(s) 370
MslI CAYNNNNRTG 2 cut(s) 249, 283
Msp20I TGGCCA 1 cut(s) 314
MspI CCGG 1 cut(s) 360
MspR9I CCNGG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 2 cut(s) 118, 225
NarI GGCGCC 1 cut(s) 357
NcoI CCATGG 1 cut(s) 210
NdeII GATC 3 cut(s) 126, 131, 362
NlaIII CATG 4 cut(s) 31, 40, 214, 288
NlaIV GGNNCC 2 cut(s) 105, 358
NmuCI GTSAC 1 cut(s) 149
PagI TCATGA 1 cut(s) 36
PluTI GGCGCC 1 cut(s) 360
Psp124BI GAGCTC 1 cut(s) 36
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 2 cut(s) 105, 358
PspPI GGNCC 1 cut(s) 164
RsaI GTAC 2 cut(s) 205, 333
RsaNI GTAC 2 cut(s) 204, 332
RseI CAYNNNNRTG 2 cut(s) 249, 283
SacI GAGCTC 1 cut(s) 36
SaqAI TTAA 1 cut(s) 370
Sau3AI GATC 3 cut(s) 126, 131, 362
Sau96I GGNCC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 1 cut(s) 36
SetI ASST 6 cut(s) 27, 36, 123, 163, 225, 230
SfaNI GCATC 1 cut(s) 149
SfoI GGCGCC 1 cut(s) 358
SmiMI CAYNNNNRTG 2 cut(s) 249, 283
SmlI CTYRAG 1 cut(s) 13
SmoI CTYRAG 1 cut(s) 13
Sse9I AATT 4 cut(s) 63, 267, 306, 367
SspDI GGCGCC 1 cut(s) 356
SstI GAGCTC 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 2 cut(s) 210, 315
TaaI ACNGT 1 cut(s) 155
TaqI TCGA 1 cut(s) 192
TasI AATT 4 cut(s) 63, 267, 306, 367
TatI WGTACW 1 cut(s) 331
Tru1I TTAA 1 cut(s) 370
Tru9I TTAA 1 cut(s) 370
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 1 cut(s) 149
Tsp45I GTSAC 1 cut(s) 149
TspDTI ATGAA 1 cut(s) 311
TspRI CASTG 1 cut(s) 151
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.