Rh4CG111700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0025850
Physical Location & Seq
Reverse (-)
21004031 .. 21007373
3343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG111700.1

Sequence Viewer

Length: 474 bp
ATGTTGATTTCGAAATTGAACTTGTTCCAAGATCATCCAGAAACTACAGTAGTATTCCAACCATGCAATTTAGGATGTCCTTATGTTCAATATCGGATGAAGCCGACTGGATTTCAGAGTGGGAAGGTCATGGAGCTCATGACAAGTCAAGAGGGAACAGAAGGAATTGGATATTGGTTTTGGAAGATGGTCTCTGCAACTTTGGAGCCTCAGCCTCACCAGCTTATGATCAGATCAACTGCATCCACTGGTGACTGTCCAGGTGGCCCTTCTTCAGTTTATGGTGGTGATTTCGACATCACCAGTACGACCATGGACTGCAACCTGAGCTTTCTTCAAGATATCCACAACTATGGAGAAGTTTCCAGAATTGTCTCATCATCATCATGGAGGACTATGAAGCAGAAAATTGTGGCCAAGGAGGAAACTGATGTACAAGAAGAAAGAGGAATATGGGGCGCCGGATCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

17.5

Weight (kDa)

5.11

Isoelectric Point (pI)

38.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025850)

Species Orthologous Gene IDs
rosa_roxburghii Rroxscaffold_5G00345590
rosa_samantha Rh4AG103400 Rh4CG111700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 458
AclWI GGATC 1 cut(s) 472
AcoI YGGCCR 1 cut(s) 414
AcuI CTGAAG 1 cut(s) 258
AcyI GRCGYC 1 cut(s) 459
AfaI GTAC 2 cut(s) 307, 435
AgsI TTSAA 3 cut(s) 19, 89, 338
AjnI CCWGG 1 cut(s) 259
AjuI GAANNNNNNNTTGG 2 cut(s) 157, 189
AluBI AGCT 3 cut(s) 136, 223, 330
AluI AGCT 3 cut(s) 136, 223, 330
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 2 cut(s) 196, 379
AlwI GGATC 1 cut(s) 472
AoxI GGCC 2 cut(s) 265, 414
Asp700I GAANNNNTTC 1 cut(s) 23
AspLEI GCGC 1 cut(s) 461
AspS9I GGNCC 1 cut(s) 266
AsuHPI GGTGA 4 cut(s) 209, 263, 292, 299
AsuII TTCGAA 1 cut(s) 11
BalI TGGCCA 1 cut(s) 416
BanI GGYRCC 1 cut(s) 458
BanII GRGCYC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 138
BbvCI CCTCAGC 1 cut(s) 210
BccI CCATC 1 cut(s) 181
BciT130I CCWGG 1 cut(s) 261
BclI TGATCA 1 cut(s) 228
BcoDI GTCTC 2 cut(s) 196, 379
BfmI CTRYAG 1 cut(s) 45
BfoI RGCGCY 1 cut(s) 462
Bme1390I CCNGG 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 266
BmiI GGNNCC 2 cut(s) 207, 460
BmrFI CCNGG 1 cut(s) 261
BmsI GCATC 1 cut(s) 251
Bpu10I CCTNAGC 2 cut(s) 210, 326
Bpu14I TTCGAA 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 459
BsaI GGTCTC 1 cut(s) 196
BsaJI CCNNGG 2 cut(s) 312, 417
Bse1I ACTGG 3 cut(s) 112, 253, 303
BseBI CCWGG 1 cut(s) 261
BseDI CCNNGG 2 cut(s) 312, 417
BseGI GGATG 4 cut(s) 34, 80, 102, 242
BseMII CTCAG 2 cut(s) 224, 317
BseNI ACTGG 3 cut(s) 112, 253, 303
BshFI GGCC 2 cut(s) 267, 416
BshNI GGYRCC 1 cut(s) 458
BsiHKAI GWGCWC 1 cut(s) 138
BsiSI CCGG 1 cut(s) 462
BsmAI GTCTC 2 cut(s) 196, 379
BsnI GGCC 2 cut(s) 267, 416
Bso31I GGTCTC 1 cut(s) 196
Bsp119I TTCGAA 1 cut(s) 11
Bsp1286I GDGCHC 1 cut(s) 138
Bsp1407I TGTACA 1 cut(s) 433
Bsp143I GATC 4 cut(s) 31, 228, 233, 464
Bsp19I CCATGG 1 cut(s) 312
BspANI GGCC 2 cut(s) 267, 416
BspCNI CTCAG 2 cut(s) 223, 318
BspHI TCATGA 1 cut(s) 138
BspLI GGNNCC 2 cut(s) 207, 460
BspPI GGATC 1 cut(s) 472
BspT104I TTCGAA 1 cut(s) 11
BspT107I GGYRCC 1 cut(s) 458
BspTNI GGTCTC 1 cut(s) 196
BsrGI TGTACA 1 cut(s) 433
BsrI ACTGG 3 cut(s) 112, 253, 303
BssECI CCNNGG 2 cut(s) 312, 417
BssMI GATC 4 cut(s) 31, 228, 233, 464
BssNI GRCGYC 1 cut(s) 459
BssT1I CCWWGG 2 cut(s) 312, 417
Bst2UI CCWGG 1 cut(s) 261
Bst4CI ACNGT 2 cut(s) 49, 257
BstACI GRCGYC 1 cut(s) 459
BstAUI TGTACA 1 cut(s) 433
BstBI TTCGAA 1 cut(s) 11
BstDEI CTNAG 2 cut(s) 210, 326
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 4 cut(s) 34, 80, 102, 242
BstH2I RGCGCY 1 cut(s) 462
BstHHI GCGC 1 cut(s) 461
BstKTI GATC 4 cut(s) 34, 231, 236, 467
BstMAI GTCTC 2 cut(s) 196, 379
BstMBI GATC 4 cut(s) 31, 228, 233, 464
BstMWI GCNNNNNNNGC 2 cut(s) 220, 327
BstNI CCWGG 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 259
BstSFI CTRYAG 1 cut(s) 45
BstXI CCANNNNNNTGG 1 cut(s) 353
BsuRI GGCC 2 cut(s) 267, 416
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 4 cut(s) 34, 80, 102, 242
BtsIMutI CAGTG 1 cut(s) 246
CciI TCATGA 1 cut(s) 138
CfoI GCGC 1 cut(s) 461
Cfr13I GGNCC 1 cut(s) 266
Csp6I GTAC 2 cut(s) 306, 434
CviAII CATG 5 cut(s) 63, 130, 139, 313, 387
CviJI RGCY 8 cut(s) 103, 136, 208, 214, 223, 267, 330, 416
CviKI_1 RGCY 8 cut(s) 103, 136, 208, 214, 223, 267, 330, 416
CviQI GTAC 2 cut(s) 306, 434
DdeI CTNAG 2 cut(s) 210, 326
DinI GGCGCC 1 cut(s) 460
DpnI GATC 4 cut(s) 33, 230, 235, 466
DpnII GATC 4 cut(s) 31, 228, 233, 464
EaeI YGGCCR 1 cut(s) 414
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 2 cut(s) 312, 417
Eco24I GRGCYC 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 196
Eco32I GATATC 1 cut(s) 343
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 258
EcoICRI GAGCTC 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 259
EcoRV GATATC 1 cut(s) 343
EcoT14I CCWWGG 2 cut(s) 312, 417
EcoT38I GRGCYC 1 cut(s) 138
EgeI GGCGCC 1 cut(s) 460
EheI GGCGCC 1 cut(s) 460
ErhI CCWWGG 2 cut(s) 312, 417
FaeI CATG 5 cut(s) 66, 133, 142, 316, 390
FatI CATG 5 cut(s) 62, 129, 138, 312, 386
FbaI TGATCA 1 cut(s) 228
FokI GGATG 4 cut(s) 21, 87, 109, 229
FriOI GRGCYC 1 cut(s) 138
GlaI GCGC 1 cut(s) 460
HaeII RGCGCY 1 cut(s) 462
HaeIII GGCC 2 cut(s) 267, 416
HapII CCGG 1 cut(s) 462
HhaI GCGC 1 cut(s) 461
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 5 cut(s) 66, 133, 142, 316, 390
Hin6I GCGC 1 cut(s) 459
HinP1I GCGC 1 cut(s) 459
HpaII CCGG 1 cut(s) 462
HphI GGTGA 4 cut(s) 209, 263, 292, 299
Hpy188I TCNGA 3 cut(s) 96, 117, 233
Hpy188III TCNNGA 5 cut(s) 38, 139, 149, 338, 366
HpyAV CCTTC 3 cut(s) 118, 155, 279
HpyCH4III ACNGT 2 cut(s) 49, 257
HpyCH4V TGCA 4 cut(s) 66, 197, 242, 321
HpyF10VI GCNNNNNNNGC 2 cut(s) 220, 327
HpyF3I CTNAG 2 cut(s) 210, 326
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 5 cut(s) 66, 133, 142, 316, 390
HspAI GCGC 1 cut(s) 459
KasI GGCGCC 1 cut(s) 458
Ksp22I TGATCA 1 cut(s) 228
Kzo9I GATC 4 cut(s) 31, 228, 233, 464
LmnI GCTCC 2 cut(s) 133, 205
LpnPI CCDG 9 cut(s) 51, 93, 233, 234, 246, 273, 316, 338, 379
LweI GCATC 1 cut(s) 251
MaeIII GTNAC 1 cut(s) 251
MalI GATC 4 cut(s) 33, 230, 235, 466
MboI GATC 4 cut(s) 31, 228, 233, 464
MboII GAAGA 4 cut(s) 196, 264, 326, 452
MhlI GDGCHC 1 cut(s) 138
MlsI TGGCCA 1 cut(s) 416
MluCI AATT 6 cut(s) 14, 67, 165, 369, 408, 469
MluNI TGGCCA 1 cut(s) 416
Mly113I GGCGCC 1 cut(s) 459
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 6 cut(s) 145, 219, 225, 384, 415, 440
Mox20I TGGCCA 1 cut(s) 416
MroXI GAANNNNTTC 1 cut(s) 23
MscI TGGCCA 1 cut(s) 416
MseI TTAA 1 cut(s) 472
MslI CAYNNNNRTG 2 cut(s) 351, 385
Msp20I TGGCCA 1 cut(s) 416
MspI CCGG 1 cut(s) 462
MspR9I CCNGG 1 cut(s) 261
MvaI CCWGG 1 cut(s) 261
MwoI GCNNNNNNNGC 2 cut(s) 220, 327
NarI GGCGCC 1 cut(s) 459
NcoI CCATGG 1 cut(s) 312
NdeII GATC 4 cut(s) 31, 228, 233, 464
NlaIII CATG 5 cut(s) 66, 133, 142, 316, 390
NlaIV GGNNCC 2 cut(s) 207, 460
NmuCI GTSAC 1 cut(s) 251
NspV TTCGAA 1 cut(s) 11
PagI TCATGA 1 cut(s) 138
PdmI GAANNNNTTC 1 cut(s) 23
PluTI GGCGCC 1 cut(s) 462
Psp124BI GAGCTC 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 259
PspGI CCWGG 1 cut(s) 259
PspN4I GGNNCC 2 cut(s) 207, 460
PspPI GGNCC 1 cut(s) 266
RsaI GTAC 2 cut(s) 307, 435
RsaNI GTAC 2 cut(s) 306, 434
RseI CAYNNNNRTG 2 cut(s) 351, 385
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 1 cut(s) 472
Sau3AI GATC 4 cut(s) 31, 228, 233, 464
Sau96I GGNCC 1 cut(s) 266
ScrFI CCNGG 1 cut(s) 261
SduI GDGCHC 1 cut(s) 138
SetI ASST 6 cut(s) 129, 138, 225, 265, 327, 332
SfaNI GCATC 1 cut(s) 251
SfcI CTRYAG 1 cut(s) 45
SfoI GGCGCC 1 cut(s) 460
SfuI TTCGAA 1 cut(s) 11
SmiMI CAYNNNNRTG 2 cut(s) 351, 385
Sse9I AATT 6 cut(s) 14, 67, 165, 369, 408, 469
SspDI GGCGCC 1 cut(s) 458
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 1 cut(s) 259
StyI CCWWGG 2 cut(s) 312, 417
TaaI ACNGT 2 cut(s) 49, 257
TaqI TCGA 2 cut(s) 11, 294
TasI AATT 6 cut(s) 14, 67, 165, 369, 408, 469
TatI WGTACW 1 cut(s) 433
Tru1I TTAA 1 cut(s) 472
Tru9I TTAA 1 cut(s) 472
TscAI CASTG 1 cut(s) 253
TseFI GTSAC 1 cut(s) 251
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 2 cut(s) 113, 413
TspRI CASTG 1 cut(s) 253
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XmnI GAANNNNTTC 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.