Rh4AG128600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0024360
Physical Location & Seq
Reverse (-)
27918697 .. 27919287
591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG128600.1

Sequence Viewer

Length: 591 bp
ATGCATGACGTGGGTGAAGGCCACGGTGTCGAAGAGCTTGTCGAGGAGTTTGTTGTCGTTTATATCTCCCTCGACGATGAAGATGCCGTGGCCTTTGAGGAGGTTCTTTCGGGCCTTCTTCAACGATGGGTCGTAGTAGCTGTTGAAGTTGTCAAGCCCCACGACGCCGTCTCCGCGCTTTTTCAGGGCCATGGACACGTGGGTGCCGACGAAACCGGCCGCCCCGGTAACCAGAACGGACATGCCGCCGGAGCGGTGGATCTGGGCGGAGTTGCGGACCTGCTTCTCCCACTGGATTCCTCCCCAGGAGGCCGAGAAGTACCGGCTGCCGGAGTCGACGAAGCTCTGGAAACTCAAATACGACGCCGTAAGGGCGATCAAGAAGAGGGCCCACAAGAACATGGTGCTTGTGGAGGCGAAGCAGCGGTGGAACTGGCGGTTCATGGTGTGGGCCCGCTCGATCTTGAACTTTCCCGGCGTCGACGGGAAGAGCTCGTCTTCCAATTGCGACGGAGACGGCATTCTTCTCTGATTATTCGTAATCGCGGAAACTCTGGGTTGTTCCTAAATTTTGTTTTTTTTCTATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

21.14

Weight (kDa)

4.92

Isoelectric Point (pI)

55.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024360)

Species Orthologous Gene IDs
malus_domestica MD02G1207700.v1.1
pyrus_communis pycom07g10480
rosa_samantha Rh4AG128600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 288
AccB1I GGYRCC 1 cut(s) 203
AccBSI CCGCTC 2 cut(s) 254, 457
AccI GTMKAC 2 cut(s) 336, 481
AccII CGCG 2 cut(s) 176, 546
AclWI GGATC 1 cut(s) 267
AcoI YGGCCR 1 cut(s) 217
AcsI RAATTY 1 cut(s) 568
AcvI CACGTG 1 cut(s) 199
AcyI GRCGYC 3 cut(s) 165, 364, 478
AfaI GTAC 1 cut(s) 321
AfiI CCNNNNNNNGG 1 cut(s) 329
AflIII ACRYGT 1 cut(s) 196
AgsI TTSAA 3 cut(s) 122, 146, 467
AjiI CACGTC 1 cut(s) 10
AjnI CCWGG 1 cut(s) 304
AleI CACNNNNGTG 1 cut(s) 201
AluBI AGCT 4 cut(s) 37, 140, 344, 493
AluI AGCT 4 cut(s) 37, 140, 344, 493
Alw21I GWGCWC 1 cut(s) 495
Alw26I GTCTC 2 cut(s) 175, 508
AlwI GGATC 1 cut(s) 267
AoxI GGCC 8 cut(s) 19, 90, 112, 187, 217, 310, 388, 451
ApaI GGGCCC 2 cut(s) 392, 455
ApeKI GCWGC 2 cut(s) 326, 422
ApoI RAATTY 1 cut(s) 568
ArsI GACNNNNNNTTYG 2 cut(s) 24, 56
AspLEI GCGC 1 cut(s) 178
AspS9I GGNCC 7 cut(s) 112, 187, 277, 388, 389, 451, 452
AsuC2I CCSGG 2 cut(s) 225, 475
AsuHPI GGTGA 1 cut(s) 26
AvaII GGWCC 1 cut(s) 277
BaeGI GKGCMC 2 cut(s) 392, 455
BanI GGYRCC 1 cut(s) 203
BanII GRGCYC 3 cut(s) 392, 455, 495
BbrPI CACGTG 1 cut(s) 199
BbsI GAAGAC 1 cut(s) 490
Bbv12I GWGCWC 1 cut(s) 495
BbvI GCAGC 2 cut(s) 313, 434
BccI CCATC 1 cut(s) 120
BceAI ACGGC 4 cut(s) 71, 152, 351, 533
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 1 cut(s) 306
BcnI CCSGG 2 cut(s) 225, 475
BcoDI GTCTC 2 cut(s) 175, 508
BfuAI ACCTGC 1 cut(s) 288
BglI GCCNNNNNGGC 1 cut(s) 372
BisI GCNGC 4 cut(s) 220, 246, 327, 423
BlsI GCNGC 4 cut(s) 221, 247, 328, 424
Bme1390I CCNGG 3 cut(s) 225, 306, 475
Bme18I GGWCC 1 cut(s) 277
BmgBI CACGTC 1 cut(s) 10
BmgT120I GGNCC 7 cut(s) 112, 187, 277, 388, 389, 451, 452
BmiI GGNNCC 3 cut(s) 205, 390, 453
BmrFI CCNGG 3 cut(s) 225, 306, 475
BmsI GCATC 1 cut(s) 73
BpiI GAAGAC 1 cut(s) 490
BpuMI CCSGG 2 cut(s) 225, 475
BsaAI YACGTR 1 cut(s) 199
BsaHI GRCGYC 3 cut(s) 165, 364, 478
BsaJI CCNNGG 5 cut(s) 22, 87, 190, 223, 304
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 1 cut(s) 329
Bse118I RCCGGY 2 cut(s) 215, 322
Bse1I ACTGG 2 cut(s) 297, 438
BseBI CCWGG 1 cut(s) 306
BseDI CCNNGG 5 cut(s) 22, 87, 190, 223, 304
BseLI CCNNNNNNNGG 1 cut(s) 329
BseNI ACTGG 2 cut(s) 297, 438
BseRI GAGGAG 2 cut(s) 59, 113
BseSI GKGCMC 2 cut(s) 392, 455
BseX3I CGGCCG 1 cut(s) 217
BseXI GCAGC 2 cut(s) 313, 434
Bsh1236I CGCG 2 cut(s) 176, 546
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 8 cut(s) 21, 92, 114, 189, 219, 312, 390, 453
BshNI GGYRCC 1 cut(s) 203
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 495
BsiSI CCGG 6 cut(s) 216, 225, 249, 323, 330, 475
BslI CCNNNNNNNGG 1 cut(s) 329
BsmAI GTCTC 2 cut(s) 175, 508
BsmBI CGTCTC 2 cut(s) 175, 508
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 8 cut(s) 21, 92, 114, 189, 219, 312, 390, 453
Bsp120I GGGCCC 2 cut(s) 388, 451
Bsp1286I GDGCHC 3 cut(s) 392, 455, 495
Bsp143I GATC 3 cut(s) 259, 376, 460
Bsp19I CCATGG 1 cut(s) 190
BspANI GGCC 8 cut(s) 21, 92, 114, 189, 219, 312, 390, 453
BspFNI CGCG 2 cut(s) 176, 546
BspLI GGNNCC 3 cut(s) 205, 390, 453
BspMI ACCTGC 1 cut(s) 288
BspPI GGATC 1 cut(s) 267
BspQI GCTCTTC 2 cut(s) 27, 483
BspT107I GGYRCC 1 cut(s) 203
BsrBI CCGCTC 2 cut(s) 254, 457
BsrFI RCCGGY 2 cut(s) 215, 322
BsrI ACTGG 2 cut(s) 297, 438
BssAI RCCGGY 2 cut(s) 215, 322
BssECI CCNNGG 5 cut(s) 22, 87, 190, 223, 304
BssMI GATC 3 cut(s) 259, 376, 460
BssNI GRCGYC 3 cut(s) 165, 364, 478
BssT1I CCWWGG 1 cut(s) 190
Bst2UI CCWGG 1 cut(s) 306
Bst4CI ACNGT 1 cut(s) 26
Bst6I CTCTTC 3 cut(s) 27, 378, 483
BstACI GRCGYC 3 cut(s) 165, 364, 478
BstBAI YACGTR 1 cut(s) 199
BstC8I GCNNGC 1 cut(s) 455
BstDSI CCRYGG 3 cut(s) 22, 87, 190
BstEII GGTNACC 1 cut(s) 227
BstFNI CGCG 2 cut(s) 176, 546
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 3 cut(s) 262, 379, 463
BstMAI GTCTC 2 cut(s) 175, 508
BstMBI GATC 3 cut(s) 259, 376, 460
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 3 cut(s) 173, 251, 372
BstNI CCWGG 1 cut(s) 306
BstNSI RCATGY 1 cut(s) 245
BstPI GGTNACC 1 cut(s) 227
BstSCI CCNGG 3 cut(s) 223, 304, 473
BstSLI GKGCMC 2 cut(s) 392, 455
BstUI CGCG 2 cut(s) 176, 546
BstV1I GCAGC 2 cut(s) 313, 434
BstV2I GAAGAC 1 cut(s) 490
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
BstZI CGGCCG 1 cut(s) 217
BsuRI GGCC 8 cut(s) 21, 92, 114, 189, 219, 312, 390, 453
BtgI CCRYGG 3 cut(s) 22, 87, 190
BtrI CACGTC 1 cut(s) 10
BtsIMutI CAGTG 1 cut(s) 290
BveI ACCTGC 1 cut(s) 288
Cac8I GCNNGC 1 cut(s) 455
CfoI GCGC 1 cut(s) 178
Cfr10I RCCGGY 2 cut(s) 215, 322
Cfr13I GGNCC 7 cut(s) 112, 187, 277, 388, 389, 451, 452
CseI GACGC 3 cut(s) 173, 372, 467
Csp6I GTAC 1 cut(s) 320
CviAII CATG 5 cut(s) 5, 191, 242, 401, 443
CviQI GTAC 1 cut(s) 320
DpnI GATC 3 cut(s) 261, 378, 462
DpnII GATC 3 cut(s) 259, 376, 460
EaeI YGGCCR 1 cut(s) 217
EagI CGGCCG 1 cut(s) 217
Eam1104I CTCTTC 3 cut(s) 27, 378, 483
EarI CTCTTC 3 cut(s) 27, 378, 483
EciI GGCGGA 1 cut(s) 282
Ecl136II GAGCTC 1 cut(s) 493
EclXI CGGCCG 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 190
Eco24I GRGCYC 3 cut(s) 392, 455, 495
Eco47I GGWCC 1 cut(s) 277
Eco52I CGGCCG 1 cut(s) 217
Eco53kI GAGCTC 1 cut(s) 493
Eco72I CACGTG 1 cut(s) 199
Eco91I GGTNACC 1 cut(s) 227
EcoICRI GAGCTC 1 cut(s) 493
EcoO109I RGGNCCY 1 cut(s) 388
EcoO65I GGTNACC 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 304
EcoT14I CCWWGG 1 cut(s) 190
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 3 cut(s) 392, 455, 495
ErhI CCWWGG 1 cut(s) 190
Esp3I CGTCTC 2 cut(s) 175, 508
FaeI CATG 5 cut(s) 8, 194, 245, 404, 446
FaiI YATR 6 cut(s) 6, 63, 192, 243, 402, 444
FatI CATG 5 cut(s) 4, 190, 241, 400, 442
FauI CCCGC 1 cut(s) 462
FblI GTMKAC 2 cut(s) 336, 481
Fnu4HI GCNGC 4 cut(s) 220, 246, 327, 423
FriOI GRGCYC 3 cut(s) 392, 455, 495
Fsp4HI GCNGC 4 cut(s) 220, 246, 327, 423
GlaI GCGC 1 cut(s) 177
GluI GCNGC 4 cut(s) 220, 246, 327, 423
HaeIII GGCC 8 cut(s) 21, 92, 114, 189, 219, 312, 390, 453
HapII CCGG 6 cut(s) 216, 225, 249, 323, 330, 475
HgaI GACGC 3 cut(s) 173, 372, 467
HhaI GCGC 1 cut(s) 178
Hin1I GRCGYC 3 cut(s) 165, 364, 478
Hin1II CATG 5 cut(s) 8, 194, 245, 404, 446
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HincII GTYRAC 2 cut(s) 337, 482
HindII GTYRAC 2 cut(s) 337, 482
HinfI GANTC 2 cut(s) 296, 333
HpaII CCGG 6 cut(s) 216, 225, 249, 323, 330, 475
HphI GGTGA 1 cut(s) 26
Hpy166II GTNNAC 2 cut(s) 337, 482
Hpy188I TCNGA 1 cut(s) 531
Hpy188III TCNNGA 4 cut(s) 347, 380, 464, 588
Hpy8I GTNNAC 2 cut(s) 337, 482
Hpy99I CGWCG 8 cut(s) 77, 167, 212, 341, 366, 483, 486, 513
HpyAV CCTTC 2 cut(s) 11, 125
HpyCH4III ACNGT 1 cut(s) 26
HpyCH4IV ACGT 2 cut(s) 9, 198
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 251, 372
HpySE526I ACGT 2 cut(s) 9, 198
Hsp92I GRCGYC 3 cut(s) 165, 364, 478
Hsp92II CATG 5 cut(s) 8, 194, 245, 404, 446
HspAI GCGC 1 cut(s) 176
Kzo9I GATC 3 cut(s) 259, 376, 460
LguI GCTCTTC 2 cut(s) 27, 483
LmnI GCTCC 1 cut(s) 251
Lsp1109I GCAGC 2 cut(s) 313, 434
LweI GCATC 1 cut(s) 73
MaeII ACGT 2 cut(s) 9, 198
MaeIII GTNAC 1 cut(s) 227
MalI GATC 3 cut(s) 261, 378, 462
MbiI CCGCTC 2 cut(s) 254, 457
MboI GATC 3 cut(s) 259, 376, 460
MboII GAAGA 7 cut(s) 44, 92, 110, 395, 490, 500, 516
MfeI CAATTG 1 cut(s) 503
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 3 cut(s) 392, 455, 495
MluCI AATT 2 cut(s) 503, 568
MlyI GAGTC 1 cut(s) 342
MnlI CCTC 8 cut(s) 37, 80, 91, 94, 302, 310, 379, 407
Mph1103I ATGCAT 1 cut(s) 6
MslI CAYNNNNRTG 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 425
MspI CCGG 6 cut(s) 216, 225, 249, 323, 330, 475
MspR9I CCNGG 3 cut(s) 225, 306, 475
MunI CAATTG 1 cut(s) 503
Mva1269I GAATGC 1 cut(s) 520
MvaI CCWGG 1 cut(s) 306
MvnI CGCG 2 cut(s) 176, 546
MwoI GCNNNNNNNGC 3 cut(s) 173, 251, 372
NciI CCSGG 2 cut(s) 225, 475
NcoI CCATGG 1 cut(s) 190
NdeII GATC 3 cut(s) 259, 376, 460
NlaIII CATG 5 cut(s) 8, 194, 245, 404, 446
NlaIV GGNNCC 3 cut(s) 205, 390, 453
NmeAIII GCCGAG 1 cut(s) 338
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 245
OliI CACNNNNGTG 1 cut(s) 201
PciSI GCTCTTC 2 cut(s) 27, 483
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PctI GAATGC 1 cut(s) 520
PfeI GAWTC 1 cut(s) 296
PflFI GACNNNGTC 1 cut(s) 167
PkrI GCNGC 4 cut(s) 221, 247, 328, 424
PleI GAGTC 1 cut(s) 341
PmaCI CACGTG 1 cut(s) 199
PmlI CACGTG 1 cut(s) 199
PpsI GAGTC 1 cut(s) 341
Ppu21I YACGTR 1 cut(s) 199
Psp124BI GAGCTC 1 cut(s) 495
Psp6I CCWGG 1 cut(s) 304
PspCI CACGTG 1 cut(s) 199
PspEI GGTNACC 1 cut(s) 227
PspGI CCWGG 1 cut(s) 304
PspN4I GGNNCC 3 cut(s) 205, 390, 453
PspOMI GGGCCC 2 cut(s) 388, 451
PspPI GGNCC 7 cut(s) 112, 187, 277, 388, 389, 451, 452
PsuI RGATCY 1 cut(s) 259
PsyI GACNNNGTC 1 cut(s) 167
RsaI GTAC 1 cut(s) 321
RsaNI GTAC 1 cut(s) 320
RseI CAYNNNNRTG 1 cut(s) 201
SacI GAGCTC 1 cut(s) 495
SalI GTCGAC 2 cut(s) 335, 480
SapI GCTCTTC 2 cut(s) 27, 483
SatI GCNGC 4 cut(s) 220, 246, 327, 423
Sau3AI GATC 3 cut(s) 259, 376, 460
Sau96I GGNCC 7 cut(s) 112, 187, 277, 388, 389, 451, 452
SchI GAGTC 1 cut(s) 342
ScrFI CCNGG 3 cut(s) 225, 306, 475
SduI GDGCHC 3 cut(s) 392, 455, 495
SetI ASST 8 cut(s) 12, 39, 105, 142, 201, 282, 346, 495
SfaNI GCATC 1 cut(s) 73
SgrDI CGTCGACG 1 cut(s) 480
SinI GGWCC 1 cut(s) 277
SmiMI CAYNNNNRTG 1 cut(s) 201
Sse9I AATT 2 cut(s) 503, 568
SstI GAGCTC 1 cut(s) 495
StyD4I CCNGG 3 cut(s) 223, 304, 473
StyI CCWWGG 1 cut(s) 190
TaaI ACNGT 1 cut(s) 26
TaiI ACGT 2 cut(s) 12, 201
TaqI TCGA 6 cut(s) 30, 42, 72, 336, 459, 481
TasI AATT 2 cut(s) 503, 568
TauI GCSGC 2 cut(s) 222, 248
TfiI GAWTC 1 cut(s) 296
TscAI CASTG 1 cut(s) 297
TseI GCWGC 2 cut(s) 326, 422
TspDTI ATGAA 2 cut(s) 93, 431
TspGWI ACGGA 2 cut(s) 252, 526
TspRI CASTG 1 cut(s) 297
Tth111I GACNNNGTC 1 cut(s) 167
VpaK11BI GGWCC 1 cut(s) 277
XapI RAATTY 1 cut(s) 568
XceI RCATGY 1 cut(s) 245
XmiI GTMKAC 2 cut(s) 336, 481
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.