Rh4AG152900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0017756
Physical Location & Seq
Forward (+)
38654728 .. 38656119
1392 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG152900.1

Sequence Viewer

Length: 594 bp
ATGGAACTTACAGAGCTCTCTTTAGCTCCAACGATATCTGCAGAAAGCAAGAACATGAATACTAGCAGGAAGAAGAGGAAGCTTTTTGAAGATGATTACCTCCTGATGAAGATTAATGAAACATCAGTAGTTCGTACAAGTCATGTTGATCTCGAGCTTGAAAACCCTTTGCCATTGGATTGGGAGCAGTGCCTTGATCTTAAGTCGGGAAAAGTGTACTATTTGAACAGAAAGACATCGGAAAAGAGTTGGAAGCGGCCATTAAAGGAGGATCAGGAGGAAGCACATCTGGACCTTGAGCTCCACATCTCGAGCACCTTCTCGGCCGATGATTCGTGTTCGGAGCAGCGACGTTGCCGAAGAAGCTCAAAGAAAGTAACTGCCGAAGAGGCGGTGATCGGCAGTAGCAATAATAATATGATGCTGGCTTTGGCTTGTTTGAATTGCCATCTGCTTGTGATACTCTCCAAGTCTTCTCCTTGTTGCCCCAACTGCAAATATGTCCACTCATTTCCACTTCCAAGTGCCAACCAAAATCTCAACTCAAGTCCTTCAACTTGGTCGATCGAGCTAGCATTATCTAACTCAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

22.28

Weight (kDa)

5.99

Isoelectric Point (pI)

58.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WW PF00397 57 - 87 1.3e-07 WW domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017756)

Species Orthologous Gene IDs
prunus_persica Prupe.1G182000_v2.0.a1
pyrus_communis pycom13g28980
rosa_chinensis RchiOBHm_Chr4g0408971
rosa_laevigata RLG00000008603
rosa_multiflora Rmu_sc0001631.1_g000024
rosa_roxburghii Rroxscaffold_5G00353400
rosa_rugosa Rorug04G0088800
rosa_samantha Rh4AG152900 Rh4BG150500 Rh4CG161300 Rh4DG146300
rosa_wichuraiana Rw4G012600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 256, 392
AclWI GGATC 1 cut(s) 279
AcoI YGGCCR 2 cut(s) 257, 324
AfaI GTAC 2 cut(s) 136, 218
AflII CTTAAG 1 cut(s) 200
AgsI TTSAA 5 cut(s) 89, 161, 226, 442, 555
AluBI AGCT 7 cut(s) 16, 26, 82, 157, 301, 366, 571
AluI AGCT 7 cut(s) 16, 26, 82, 157, 301, 366, 571
Alw21I GWGCWC 3 cut(s) 18, 303, 317
AlwI GGATC 1 cut(s) 279
Ama87I CYCGRG 2 cut(s) 152, 310
AoxI GGCC 2 cut(s) 257, 324
ApeKI GCWGC 1 cut(s) 346
AseI ATTAAT 1 cut(s) 114
AspS9I GGNCC 1 cut(s) 292
AsuHPI GGTGA 1 cut(s) 406
AsuNHI GCTAGC 1 cut(s) 571
AvaI CYCGRG 2 cut(s) 152, 310
AvaII GGWCC 1 cut(s) 292
BanII GRGCYC 2 cut(s) 18, 303
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 3 cut(s) 18, 303, 317
BbvI GCAGC 1 cut(s) 358
BccI CCATC 1 cut(s) 456
BfaI CTAG 2 cut(s) 63, 572
BfmI CTRYAG 1 cut(s) 39
BfrI CTTAAG 1 cut(s) 200
BglI GCCNNNNNGGC 1 cut(s) 389
BisI GCNGC 2 cut(s) 257, 347
BlsI GCNGC 2 cut(s) 258, 348
Bme18I GGWCC 1 cut(s) 292
BmeT110I CYCGRG 2 cut(s) 152, 310
BmgT120I GGNCC 1 cut(s) 292
BmsI GCATC 1 cut(s) 411
BmtI GCTAGC 1 cut(s) 575
BpiI GAAGAC 1 cut(s) 465
BpuEI CTTGAG 2 cut(s) 317, 529
BseX3I CGGCCG 1 cut(s) 324
BseXI GCAGC 1 cut(s) 358
Bsh1285I CGRYCG 2 cut(s) 327, 567
BshFI GGCC 2 cut(s) 259, 326
BsiEI CGRYCG 2 cut(s) 327, 567
BsiHKAI GWGCWC 3 cut(s) 18, 303, 317
BsiHKCI CYCGRG 2 cut(s) 152, 310
BsnI GGCC 2 cut(s) 259, 326
BsoBI CYCGRG 2 cut(s) 152, 310
Bsp1286I GDGCHC 3 cut(s) 18, 303, 317
Bsp143I GATC 5 cut(s) 148, 196, 271, 396, 564
BspACI CCGC 2 cut(s) 256, 392
BspANI GGCC 2 cut(s) 259, 326
BspMAI CTGCAG 1 cut(s) 43
BspOI GCTAGC 1 cut(s) 575
BspPI GGATC 1 cut(s) 279
BspTI CTTAAG 1 cut(s) 200
BssMI GATC 5 cut(s) 148, 196, 271, 396, 564
Bst6I CTCTTC 2 cut(s) 68, 381
BstAFI CTTAAG 1 cut(s) 200
BstC8I GCNNGC 2 cut(s) 426, 573
BstDEI CTNAG 1 cut(s) 586
BstKTI GATC 5 cut(s) 151, 199, 274, 399, 567
BstMBI GATC 5 cut(s) 148, 196, 271, 396, 564
BstMCI CGRYCG 2 cut(s) 327, 567
BstMWI GCNNNNNNNGC 3 cut(s) 363, 389, 492
BstSFI CTRYAG 1 cut(s) 39
BstV1I GCAGC 1 cut(s) 358
BstV2I GAAGAC 1 cut(s) 465
BstXI CCANNNNNNTGG 1 cut(s) 180
BstZI CGGCCG 1 cut(s) 324
BsuRI GGCC 2 cut(s) 259, 326
BtsI GCAGTG 1 cut(s) 194
BtsIMutI CAGTG 1 cut(s) 194
Cac8I GCNNGC 2 cut(s) 426, 573
Cfr13I GGNCC 1 cut(s) 292
Csp6I GTAC 2 cut(s) 135, 217
CviAII CATG 2 cut(s) 55, 143
CviQI GTAC 2 cut(s) 135, 217
DdeI CTNAG 1 cut(s) 586
DpnI GATC 5 cut(s) 150, 198, 273, 398, 566
DpnII GATC 5 cut(s) 148, 196, 271, 396, 564
EaeI YGGCCR 2 cut(s) 257, 324
EagI CGGCCG 1 cut(s) 324
Eam1104I CTCTTC 2 cut(s) 68, 381
EarI CTCTTC 2 cut(s) 68, 381
Ecl136II GAGCTC 2 cut(s) 16, 301
EclXI CGGCCG 1 cut(s) 324
Eco24I GRGCYC 2 cut(s) 18, 303
Eco32I GATATC 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 292
Eco52I CGGCCG 1 cut(s) 324
Eco53kI GAGCTC 2 cut(s) 16, 301
Eco88I CYCGRG 2 cut(s) 152, 310
EcoICRI GAGCTC 2 cut(s) 16, 301
EcoRV GATATC 1 cut(s) 36
EcoT38I GRGCYC 2 cut(s) 18, 303
FaeI CATG 2 cut(s) 58, 146
FaiI YATR 4 cut(s) 56, 144, 419, 501
FatI CATG 2 cut(s) 54, 142
Fnu4HI GCNGC 2 cut(s) 257, 347
FriOI GRGCYC 2 cut(s) 18, 303
Fsp4HI GCNGC 2 cut(s) 257, 347
FspBI CTAG 2 cut(s) 63, 572
GluI GCNGC 2 cut(s) 257, 347
HaeIII GGCC 2 cut(s) 259, 326
Hin1II CATG 2 cut(s) 58, 146
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 1 cut(s) 332
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 2 cut(s) 217, 505
Hpy188I TCNGA 3 cut(s) 241, 343, 589
Hpy188III TCNNGA 6 cut(s) 103, 152, 207, 275, 290, 310
Hpy8I GTNNAC 2 cut(s) 217, 505
Hpy99I CGWCG 1 cut(s) 354
HpyAV CCTTC 2 cut(s) 328, 561
HpyCH4IV ACGT 1 cut(s) 352
HpyCH4V TGCA 2 cut(s) 41, 495
HpyF10VI GCNNNNNNNGC 3 cut(s) 363, 389, 492
HpyF3I CTNAG 1 cut(s) 586
HpySE526I ACGT 1 cut(s) 352
Hsp92II CATG 2 cut(s) 58, 146
Kzo9I GATC 5 cut(s) 148, 196, 271, 396, 564
LmnI GCTCC 4 cut(s) 31, 184, 306, 343
LpnPI CCDG 5 cut(s) 52, 116, 260, 275, 410
Lsp1109I GCAGC 1 cut(s) 358
LweI GCATC 1 cut(s) 411
MaeI CTAG 2 cut(s) 63, 572
MaeII ACGT 1 cut(s) 352
MaeIII GTNAC 1 cut(s) 376
MalI GATC 5 cut(s) 150, 198, 273, 398, 566
MboI GATC 5 cut(s) 148, 196, 271, 396, 564
MboII GAAGA 7 cut(s) 82, 85, 101, 121, 372, 398, 465
MhlI GDGCHC 3 cut(s) 18, 303, 317
MluCI AATT 1 cut(s) 442
MmeI TCCRAC 2 cut(s) 53, 230
MnlI CCTC 5 cut(s) 69, 110, 262, 271, 382
MseI TTAA 3 cut(s) 114, 201, 263
MspCI CTTAAG 1 cut(s) 200
MwoI GCNNNNNNNGC 3 cut(s) 363, 389, 492
NdeII GATC 5 cut(s) 148, 196, 271, 396, 564
NheI GCTAGC 1 cut(s) 571
NlaIII CATG 2 cut(s) 58, 146
NmeAIII GCCGAG 1 cut(s) 302
PaeR7I CTCGAG 2 cut(s) 152, 310
PfeI GAWTC 1 cut(s) 332
PkrI GCNGC 2 cut(s) 258, 348
Ple19I CGATCG 1 cut(s) 567
PshBI ATTAAT 1 cut(s) 114
Psp124BI GAGCTC 2 cut(s) 18, 303
PspPI GGNCC 1 cut(s) 292
PstI CTGCAG 1 cut(s) 43
PvuI CGATCG 1 cut(s) 567
RsaI GTAC 2 cut(s) 136, 218
RsaNI GTAC 2 cut(s) 135, 217
SacI GAGCTC 2 cut(s) 18, 303
SaqAI TTAA 3 cut(s) 114, 201, 263
SatI GCNGC 2 cut(s) 257, 347
Sau3AI GATC 5 cut(s) 148, 196, 271, 396, 564
Sau96I GGNCC 1 cut(s) 292
SduI GDGCHC 3 cut(s) 18, 303, 317
SfaNI GCATC 1 cut(s) 411
SfcI CTRYAG 1 cut(s) 39
Sfr274I CTCGAG 2 cut(s) 152, 310
SinI GGWCC 1 cut(s) 292
SlaI CTCGAG 2 cut(s) 152, 310
SmlI CTYRAG 5 cut(s) 152, 200, 296, 310, 544
SmoI CTYRAG 5 cut(s) 152, 200, 296, 310, 544
Sse9I AATT 1 cut(s) 442
SsiI CCGC 2 cut(s) 256, 392
SspMI CTAG 2 cut(s) 63, 572
SstI GAGCTC 2 cut(s) 18, 303
TaiI ACGT 1 cut(s) 355
TaqI TCGA 4 cut(s) 153, 311, 563, 567
TasI AATT 1 cut(s) 442
TatI WGTACW 1 cut(s) 216
TauI GCSGC 1 cut(s) 259
TfiI GAWTC 1 cut(s) 332
Tru1I TTAA 3 cut(s) 114, 201, 263
Tru9I TTAA 3 cut(s) 114, 201, 263
TscAI CASTG 1 cut(s) 194
TseI GCWGC 1 cut(s) 346
TspDTI ATGAA 3 cut(s) 71, 122, 132
TspRI CASTG 1 cut(s) 194
Vha464I CTTAAG 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 292
VspI ATTAAT 1 cut(s) 114
XhoI CTCGAG 2 cut(s) 152, 310
XspI CTAG 2 cut(s) 63, 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.