Rh4AG154000

Chloroplastic group IIA intron splicing facilitator CRS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0021653
Physical Location & Seq
Reverse (-)
38862996 .. 38863785
790 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG154000.1

Sequence Viewer

Length: 414 bp
ATGGTGATTTCAAAAGGCTTAATTGGGGCTGCTGTGGATGAAATTCTATCTGGGGAGAGTGAAAATGATGAAAATGTGGAACCTTTTGTGTCTTCTGGTCGTGATTCTCAAGCTTCTACTCGGTTGCCTTGGGAGAGAGAAGGTGAATTGGGTAATGGGGAAGGTGGTAAGACAATAAAGAAGTGGAGTAATACGCTTTCAACTGAGGCATCCCTTCCAGAGCATGAGTTGAAGAGGCTAAGGAATGTTTATTTGAGGATGTTGGAAAGGACTAAAGTCGGAGCTGCCGGTATCACACAGGGTTTGGTGGATGCCATATATGAGAGTTGGAAGGTGGATGAAGTGGTCAAGTTGAAGTTTGAGGAGCCACTCTCACTTAACATGAAAAGAACTCATGGCATTTTAGAGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

137

Amino Acids

15.21

Weight (kDa)

5.17

Isoelectric Point (pI)

25.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 72 - 136 4.2e-10 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021653)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002466.1_g000002
rosa_samantha Rh1DG193500 Rh4AG154000 Rh4CG162600 Rh5AG090200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 42
AgsI TTSAA 4 cut(s) 12, 201, 232, 355
AluBI AGCT 3 cut(s) 113, 284, 409
AluI AGCT 3 cut(s) 113, 284, 409
ApeKI GCWGC 2 cut(s) 29, 284
ApoI RAATTY 1 cut(s) 42
AsuHPI GGTGA 2 cut(s) 16, 155
BbsI GAAGAC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 16, 271
BisI GCNGC 2 cut(s) 30, 285
BlsI GCNGC 2 cut(s) 31, 286
BmiI GGNNCC 2 cut(s) 81, 366
BmsI GCATC 2 cut(s) 218, 301
BoxI GACNNNNGTC 1 cut(s) 275
BpiI GAAGAC 1 cut(s) 84
BplI GAGNNNNNCTC 2 cut(s) 356, 388
Bpu10I CCTNAGC 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 128
Bse118I RCCGGY 1 cut(s) 287
BseDI CCNNGG 1 cut(s) 128
BseGI GGATG 5 cut(s) 43, 209, 264, 316, 343
BseMII CTCAG 1 cut(s) 195
BseRI GAGGAG 1 cut(s) 377
BseXI GCAGC 2 cut(s) 16, 271
BsiSI CCGG 1 cut(s) 288
BspCNI CTCAG 1 cut(s) 196
BspLI GGNNCC 2 cut(s) 81, 366
BsrFI RCCGGY 1 cut(s) 287
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 1 cut(s) 128
BssT1I CCWWGG 1 cut(s) 128
Bst6I CTCTTC 1 cut(s) 227
BstDEI CTNAG 2 cut(s) 204, 239
BstF5I GGATG 5 cut(s) 43, 209, 264, 316, 343
BstPAI GACNNNNGTC 1 cut(s) 275
BstV1I GCAGC 2 cut(s) 16, 271
BstV2I GAAGAC 1 cut(s) 84
BtsCI GGATG 5 cut(s) 43, 209, 264, 316, 343
Cfr10I RCCGGY 1 cut(s) 287
CviAII CATG 3 cut(s) 224, 382, 395
CviJI RGCY 7 cut(s) 18, 29, 113, 238, 284, 367, 409
CviKI_1 RGCY 7 cut(s) 18, 29, 113, 238, 284, 367, 409
DdeI CTNAG 2 cut(s) 204, 239
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Eco130I CCWWGG 1 cut(s) 128
EcoT14I CCWWGG 1 cut(s) 128
ErhI CCWWGG 1 cut(s) 128
FaeI CATG 3 cut(s) 227, 385, 398
FaiI YATR 7 cut(s) 225, 317, 319, 321, 383, 396, 412
FatI CATG 3 cut(s) 223, 381, 394
Fnu4HI GCNGC 2 cut(s) 30, 285
FokI GGATG 5 cut(s) 50, 196, 271, 323, 350
Fsp4HI GCNGC 2 cut(s) 30, 285
GluI GCNGC 2 cut(s) 30, 285
HapII CCGG 1 cut(s) 288
Hin1II CATG 3 cut(s) 227, 385, 398
HindIII AAGCTT 1 cut(s) 111
HinfI GANTC 1 cut(s) 104
HpaII CCGG 1 cut(s) 288
HphI GGTGA 2 cut(s) 16, 155
Hpy188I TCNGA 1 cut(s) 281
Hpy188III TCNNGA 2 cut(s) 101, 218
HpyAV CCTTC 4 cut(s) 134, 155, 224, 325
HpyF3I CTNAG 2 cut(s) 204, 239
Hsp92II CATG 3 cut(s) 227, 385, 398
LmnI GCTCC 2 cut(s) 281, 364
LpnPI CCDG 5 cut(s) 36, 81, 231, 284, 301
Lsp1109I GCAGC 2 cut(s) 16, 271
LweI GCATC 2 cut(s) 218, 301
MboII GAAGA 2 cut(s) 84, 244
MluCI AATT 3 cut(s) 21, 42, 146
MmeI TCCRAC 3 cut(s) 243, 259, 308
MnlI CCTC 4 cut(s) 199, 228, 249, 355
MseI TTAA 2 cut(s) 20, 378
MspI CCGG 1 cut(s) 288
NlaIII CATG 3 cut(s) 227, 385, 398
NlaIV GGNNCC 2 cut(s) 81, 366
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 2 cut(s) 31, 286
PshAI GACNNNNGTC 1 cut(s) 275
PspN4I GGNNCC 2 cut(s) 81, 366
SaqAI TTAA 2 cut(s) 20, 378
SatI GCNGC 2 cut(s) 30, 285
SetI ASST 7 cut(s) 85, 115, 145, 166, 286, 336, 411
SfaNI GCATC 2 cut(s) 218, 301
SmlI CTYRAG 1 cut(s) 108
SmoI CTYRAG 1 cut(s) 108
Sse9I AATT 3 cut(s) 21, 42, 146
StyI CCWWGG 1 cut(s) 128
TasI AATT 3 cut(s) 21, 42, 146
TfiI GAWTC 1 cut(s) 104
Tru1I TTAA 2 cut(s) 20, 378
Tru9I TTAA 2 cut(s) 20, 378
TseI GCWGC 2 cut(s) 29, 284
TspDTI ATGAA 4 cut(s) 54, 84, 354, 398
XapI RAATTY 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.