Rh5AG090200

Chloroplastic group IIA intron splicing facilitator CRS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0021653
Physical Location & Seq
Reverse (-)
8080673 .. 8101334
20662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG090200.1

Sequence Viewer

Length: 567 bp
ATGGATGGTCTTGCCTGCCTTTGTGTTGGTCCTGAGCCTCTTTGTTACCGATGTATACAATATGAATCAGTGGAATCTAACCACCAGGAGGGGATGGTGATTTCAAAAGGCTTAATTGGGGCTGCTGTGGATGAAATTCTATCTGGGGATAGTGAAAATGATGAAAATGTGGAACCTTTTGTGTCTTCTGGTCGTGATTCTCAAGCTTCTACTCGGTTGCCTTGGGAGAGAGAAGGTGAATTGGGTAATGGGGAAGGTGGTAAGATAATAAAGAAGTGGAGTAATACGCTTTCGGCTGAGGCATCCCTTCCGGAGCATGAGTTGAAGAGGCTAAGGAAGGTTCATTTGAGGATGTTGGAAAGGACTAAAGTCGGAGCTGCCGGTATCACACAGGGTTTGGTGGATGCTATACATGAGAAGTGGAAGGTGGATGAAGTGGTCAAGTTGAAGTTTGAGGAGCCACTCTCACTTAACATGAAAAGAACTCATGGCATTTTAGAGATAAGTTGCCAGATTGATGCAGAGACAATGAGTTTGGGTACAATTACAATAGCCAGAAGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

188

Amino Acids

20.7

Weight (kDa)

5.14

Isoelectric Point (pI)

31.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 103 - 170 1.9e-11 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0021653)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002466.1_g000002
rosa_samantha Rh1DG193500 Rh4AG154000 Rh4CG162600 Rh5AG090200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 55
AccIII TCCGGA 1 cut(s) 310
AcsI RAATTY 1 cut(s) 135
AfaI GTAC 1 cut(s) 541
AfiI CCNNNNNNNGG 1 cut(s) 88
AgsI TTSAA 3 cut(s) 105, 325, 448
AjnI CCWGG 1 cut(s) 84
AluBI AGCT 2 cut(s) 206, 377
AluI AGCT 2 cut(s) 206, 377
Alw26I GTCTC 1 cut(s) 518
Aor13HI TCCGGA 1 cut(s) 310
ApeKI GCWGC 2 cut(s) 122, 377
ApoI RAATTY 1 cut(s) 135
ArsI GACNNNNNNTTYG 2 cut(s) 517, 549
AspS9I GGNCC 1 cut(s) 29
AsuHPI GGTGA 2 cut(s) 109, 248
AvaII GGWCC 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 177
BbvCI CCTCAGC 1 cut(s) 297
BbvI GCAGC 2 cut(s) 109, 364
BccI CCATC 1 cut(s) 88
BciT130I CCWGG 1 cut(s) 86
BcoDI GTCTC 1 cut(s) 518
BisI GCNGC 2 cut(s) 123, 378
BlsI GCNGC 2 cut(s) 124, 379
Bme1390I CCNGG 1 cut(s) 86
Bme18I GGWCC 1 cut(s) 29
BmgT120I GGNCC 1 cut(s) 29
BmiI GGNNCC 2 cut(s) 174, 459
BmrFI CCNGG 1 cut(s) 86
BmsI GCATC 3 cut(s) 311, 394, 508
BoxI GACNNNNGTC 1 cut(s) 368
BpiI GAAGAC 1 cut(s) 177
BplI GAGNNNNNCTC 2 cut(s) 449, 481
Bpu10I CCTNAGC 3 cut(s) 33, 297, 332
BpuEI CTTGAG 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 221
BsaWI WCCGGW 1 cut(s) 310
BsaXI ACNNNNNCTCC 2 cut(s) 305, 335
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse118I RCCGGY 1 cut(s) 380
BseAI TCCGGA 1 cut(s) 310
BseBI CCWGG 1 cut(s) 86
BseDI CCNNGG 1 cut(s) 221
BseGI GGATG 7 cut(s) 10, 99, 136, 302, 357, 409, 436
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMII CTCAG 2 cut(s) 24, 288
BseRI GAGGAG 1 cut(s) 470
BseXI GCAGC 2 cut(s) 109, 364
BsiSI CCGG 2 cut(s) 311, 381
BslI CCNNNNNNNGG 1 cut(s) 88
BsmAI GTCTC 1 cut(s) 518
Bsp13I TCCGGA 1 cut(s) 310
BspCNI CTCAG 2 cut(s) 25, 289
BspEI TCCGGA 1 cut(s) 310
BspLI GGNNCC 2 cut(s) 174, 459
BsrFI RCCGGY 1 cut(s) 380
BssAI RCCGGY 1 cut(s) 380
BssECI CCNNGG 1 cut(s) 221
BssNAI GTATAC 1 cut(s) 56
BssT1I CCWWGG 1 cut(s) 221
Bst1107I GTATAC 1 cut(s) 56
Bst2UI CCWGG 1 cut(s) 86
Bst6I CTCTTC 1 cut(s) 320
BstC8I GCNNGC 1 cut(s) 16
BstDEI CTNAG 3 cut(s) 33, 297, 332
BstF5I GGATG 7 cut(s) 10, 99, 136, 302, 357, 409, 436
BstMAI GTCTC 1 cut(s) 518
BstNI CCWGG 1 cut(s) 86
BstPAI GACNNNNGTC 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 109, 364
BstV2I GAAGAC 1 cut(s) 177
BstZ17I GTATAC 1 cut(s) 56
BtsCI GGATG 7 cut(s) 10, 99, 136, 302, 357, 409, 436
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 16
Cfr10I RCCGGY 1 cut(s) 380
Cfr13I GGNCC 1 cut(s) 29
Csp6I GTAC 1 cut(s) 540
CviAII CATG 4 cut(s) 317, 413, 475, 488
CviJI RGCY 9 cut(s) 37, 111, 122, 206, 296, 331, 377, 460, 554
CviKI_1 RGCY 9 cut(s) 37, 111, 122, 206, 296, 331, 377, 460, 554
CviQI GTAC 1 cut(s) 540
DdeI CTNAG 3 cut(s) 33, 297, 332
Eam1104I CTCTTC 1 cut(s) 320
EarI CTCTTC 1 cut(s) 320
Eco130I CCWWGG 1 cut(s) 221
Eco47I GGWCC 1 cut(s) 29
EcoRII CCWGG 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 221
ErhI CCWWGG 1 cut(s) 221
FaeI CATG 4 cut(s) 320, 416, 478, 491
FaiI YATR 7 cut(s) 56, 63, 318, 410, 414, 476, 489
FatI CATG 4 cut(s) 316, 412, 474, 487
FblI GTMKAC 1 cut(s) 55
Fnu4HI GCNGC 2 cut(s) 123, 378
FokI GGATG 7 cut(s) 17, 106, 143, 289, 364, 416, 443
Fsp4HI GCNGC 2 cut(s) 123, 378
GluI GCNGC 2 cut(s) 123, 378
HapII CCGG 2 cut(s) 311, 381
Hin1II CATG 4 cut(s) 320, 416, 478, 491
HindIII AAGCTT 1 cut(s) 204
HinfI GANTC 3 cut(s) 65, 74, 197
HpaII CCGG 2 cut(s) 311, 381
HphI GGTGA 2 cut(s) 109, 248
Hpy166II GTNNAC 1 cut(s) 56
Hpy188I TCNGA 1 cut(s) 374
Hpy188III TCNNGA 3 cut(s) 32, 194, 311
Hpy8I GTNNAC 1 cut(s) 56
HpyAV CCTTC 5 cut(s) 227, 248, 317, 331, 418
HpyCH4V TGCA 1 cut(s) 521
HpyF3I CTNAG 3 cut(s) 33, 297, 332
Hsp92II CATG 4 cut(s) 320, 416, 478, 491
Kpn2I TCCGGA 1 cut(s) 310
LmnI GCTCC 3 cut(s) 313, 374, 457
Lsp1109I GCAGC 2 cut(s) 109, 364
LweI GCATC 3 cut(s) 311, 394, 508
MaeIII GTNAC 2 cut(s) 44, 560
MboII GAAGA 2 cut(s) 177, 337
MluCI AATT 4 cut(s) 114, 135, 239, 543
MmeI TCCRAC 2 cut(s) 336, 352
MnlI CCTC 6 cut(s) 48, 82, 292, 321, 342, 448
MroI TCCGGA 1 cut(s) 310
MseI TTAA 2 cut(s) 113, 471
MspI CCGG 2 cut(s) 311, 381
MspR9I CCNGG 1 cut(s) 86
MvaI CCWGG 1 cut(s) 86
NlaIII CATG 4 cut(s) 320, 416, 478, 491
NlaIV GGNNCC 2 cut(s) 174, 459
NmuCI GTSAC 1 cut(s) 560
PfeI GAWTC 3 cut(s) 65, 74, 197
PkrI GCNGC 2 cut(s) 124, 379
PshAI GACNNNNGTC 1 cut(s) 368
Psp6I CCWGG 1 cut(s) 84
PspGI CCWGG 1 cut(s) 84
PspN4I GGNNCC 2 cut(s) 174, 459
PspPI GGNCC 1 cut(s) 29
RsaI GTAC 1 cut(s) 541
RsaNI GTAC 1 cut(s) 540
SaqAI TTAA 2 cut(s) 113, 471
SatI GCNGC 2 cut(s) 123, 378
Sau96I GGNCC 1 cut(s) 29
ScrFI CCNGG 1 cut(s) 86
SetI ASST 7 cut(s) 178, 208, 238, 259, 342, 379, 429
SfaNI GCATC 3 cut(s) 311, 394, 508
SinI GGWCC 1 cut(s) 29
SmlI CTYRAG 1 cut(s) 201
SmoI CTYRAG 1 cut(s) 201
Sse9I AATT 4 cut(s) 114, 135, 239, 543
StyD4I CCNGG 1 cut(s) 84
StyI CCWWGG 1 cut(s) 221
TasI AATT 4 cut(s) 114, 135, 239, 543
TfiI GAWTC 3 cut(s) 65, 74, 197
Tru1I TTAA 2 cut(s) 113, 471
Tru9I TTAA 2 cut(s) 113, 471
TscAI CASTG 1 cut(s) 75
TseFI GTSAC 1 cut(s) 560
TseI GCWGC 2 cut(s) 122, 377
Tsp45I GTSAC 1 cut(s) 560
TspDTI ATGAA 6 cut(s) 78, 147, 177, 332, 447, 491
TspRI CASTG 1 cut(s) 75
VpaK11BI GGWCC 1 cut(s) 29
XapI RAATTY 1 cut(s) 135
XmiI GTMKAC 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.