Rh4CG162600

Chloroplastic group IIA intron splicing facilitator CRS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0021653
Physical Location & Seq
Reverse (-)
35689768 .. 35690557
790 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG162600.1

Sequence Viewer

Length: 570 bp
ATGGTGATTTCAAAAGGCTTAATTGGGGCTGCTGTGGATGAAATTCTATCTGGGGATAGTGAAAATGATGAAAATGTGGAACCTTTTGTGTCTTCTGGTCGTGATTCTCAAGCTTCTACTCGGTTGCCTTGGGAGAGAGAAGGTGAATTGGGTAATGGGGAAGGTGGTAAGACAATAAAGAAGTGGAGTAATACGCTTTCAACTGAGGCATCCCTTCCGGAGCATGAGTTGAAGAGGCTAAGGAATGTTTATTTGAGGATGTTGGAAAGGACTAAAGTCGGAGCTGCCGGTATCACACAGGGTTTGGTGGATGCCATACATGAGAGTTGGAAGGTGGATGAAGTGGTCAAGTTGAAGTTTGAGGAGCCACTCTCACTTAACATGAAAAGAACTCGTGGCATTTTAGAGAGTAAAACTGGAGGTTTGGTTATATGGAGATCAGGCAGTTCAGTAGTGTTATACAGGGGAATATCATACAATCTTCCGTGTGTAAACTCATATACCAAACATTTGGAGACTGTTGACCTATCTTTCCACTGGTTTGATTCATTTTTTCTTTTGAAGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

189

Amino Acids

21.14

Weight (kDa)

6.12

Isoelectric Point (pI)

25.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 72 - 155 3.2e-19 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021653)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002466.1_g000002
rosa_samantha Rh1DG193500 Rh4AG154000 Rh4CG162600 Rh5AG090200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 217
AcsI RAATTY 1 cut(s) 42
AgsI TTSAA 5 cut(s) 12, 201, 232, 355, 562
AluBI AGCT 3 cut(s) 113, 284, 565
AluI AGCT 3 cut(s) 113, 284, 565
Alw26I GTCTC 1 cut(s) 509
Aor13HI TCCGGA 1 cut(s) 217
ApeKI GCWGC 2 cut(s) 29, 284
ApoI RAATTY 1 cut(s) 42
AsuHPI GGTGA 2 cut(s) 16, 155
BauI CACGAG 1 cut(s) 393
BbsI GAAGAC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 16, 271
BcoDI GTCTC 1 cut(s) 509
BisI GCNGC 2 cut(s) 30, 285
BlsI GCNGC 2 cut(s) 31, 286
BmiI GGNNCC 2 cut(s) 81, 366
BmsI GCATC 2 cut(s) 218, 301
BoxI GACNNNNGTC 1 cut(s) 275
BpiI GAAGAC 1 cut(s) 84
BplI GAGNNNNNCTC 2 cut(s) 356, 388
BpmI CTGGAG 1 cut(s) 438
Bpu10I CCTNAGC 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 128
BsaWI WCCGGW 1 cut(s) 217
BsaXI ACNNNNNCTCC 4 cut(s) 212, 242, 411, 441
Bse118I RCCGGY 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 421, 542
BseAI TCCGGA 1 cut(s) 217
BseDI CCNNGG 1 cut(s) 128
BseGI GGATG 5 cut(s) 43, 209, 264, 316, 343
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 2 cut(s) 421, 542
BseRI GAGGAG 1 cut(s) 377
BseXI GCAGC 2 cut(s) 16, 271
BsiSI CCGG 2 cut(s) 218, 288
BsmAI GTCTC 1 cut(s) 509
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 1 cut(s) 437
BspCNI CTCAG 1 cut(s) 196
BspEI TCCGGA 1 cut(s) 217
BspLI GGNNCC 2 cut(s) 81, 366
BsrFI RCCGGY 1 cut(s) 287
BsrI ACTGG 2 cut(s) 421, 542
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 1 cut(s) 128
BssMI GATC 1 cut(s) 437
BssSI CACGAG 1 cut(s) 393
BssT1I CCWWGG 1 cut(s) 128
Bst2BI CACGAG 1 cut(s) 393
Bst4CI ACNGT 1 cut(s) 520
Bst6I CTCTTC 1 cut(s) 227
BstDEI CTNAG 2 cut(s) 204, 239
BstF5I GGATG 5 cut(s) 43, 209, 264, 316, 343
BstKTI GATC 1 cut(s) 440
BstMAI GTCTC 1 cut(s) 509
BstMBI GATC 1 cut(s) 437
BstPAI GACNNNNGTC 1 cut(s) 275
BstV1I GCAGC 2 cut(s) 16, 271
BstV2I GAAGAC 1 cut(s) 84
BstXI CCANNNNNNTGG 1 cut(s) 511
BtsCI GGATG 5 cut(s) 43, 209, 264, 316, 343
BtsIMutI CAGTG 1 cut(s) 535
Cfr10I RCCGGY 1 cut(s) 287
CviAII CATG 3 cut(s) 224, 320, 382
CviJI RGCY 7 cut(s) 18, 29, 113, 238, 284, 367, 565
CviKI_1 RGCY 7 cut(s) 18, 29, 113, 238, 284, 367, 565
DdeI CTNAG 2 cut(s) 204, 239
DpnI GATC 1 cut(s) 439
DpnII GATC 1 cut(s) 437
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Eco130I CCWWGG 1 cut(s) 128
EcoT14I CCWWGG 1 cut(s) 128
ErhI CCWWGG 1 cut(s) 128
FaeI CATG 3 cut(s) 227, 323, 385
FatI CATG 3 cut(s) 223, 319, 381
Fnu4HI GCNGC 2 cut(s) 30, 285
FokI GGATG 5 cut(s) 50, 196, 271, 323, 350
Fsp4HI GCNGC 2 cut(s) 30, 285
GluI GCNGC 2 cut(s) 30, 285
GsuI CTGGAG 1 cut(s) 438
HapII CCGG 2 cut(s) 218, 288
Hin1II CATG 3 cut(s) 227, 323, 385
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HindIII AAGCTT 1 cut(s) 111
HinfI GANTC 2 cut(s) 104, 545
HpaII CCGG 2 cut(s) 218, 288
HphI GGTGA 2 cut(s) 16, 155
Hpy166II GTNNAC 2 cut(s) 493, 523
Hpy188I TCNGA 1 cut(s) 281
Hpy188III TCNNGA 2 cut(s) 101, 218
Hpy8I GTNNAC 2 cut(s) 493, 523
HpyAV CCTTC 4 cut(s) 134, 155, 224, 325
HpyCH4III ACNGT 1 cut(s) 520
HpyF3I CTNAG 2 cut(s) 204, 239
Hsp92II CATG 3 cut(s) 227, 323, 385
Kpn2I TCCGGA 1 cut(s) 217
Kzo9I GATC 1 cut(s) 437
LmnI GCTCC 3 cut(s) 220, 281, 364
LpnPI CCDG 9 cut(s) 36, 81, 231, 284, 301, 402, 426, 448, 523
Lsp1109I GCAGC 2 cut(s) 16, 271
LweI GCATC 2 cut(s) 218, 301
MalI GATC 1 cut(s) 439
MboI GATC 1 cut(s) 437
MboII GAAGA 3 cut(s) 84, 244, 473
MluCI AATT 3 cut(s) 21, 42, 146
MmeI TCCRAC 3 cut(s) 243, 259, 308
MnlI CCTC 5 cut(s) 199, 228, 249, 355, 413
MroI TCCGGA 1 cut(s) 217
MseI TTAA 2 cut(s) 20, 378
MspI CCGG 2 cut(s) 218, 288
NdeII GATC 1 cut(s) 437
NlaIII CATG 3 cut(s) 227, 323, 385
NlaIV GGNNCC 2 cut(s) 81, 366
PfeI GAWTC 2 cut(s) 104, 545
PkrI GCNGC 2 cut(s) 31, 286
PshAI GACNNNNGTC 1 cut(s) 275
PspN4I GGNNCC 2 cut(s) 81, 366
SaqAI TTAA 2 cut(s) 20, 378
SatI GCNGC 2 cut(s) 30, 285
Sau3AI GATC 1 cut(s) 437
SetI ASST 9 cut(s) 85, 115, 145, 166, 286, 336, 424, 528, 567
SfaNI GCATC 2 cut(s) 218, 301
SmlI CTYRAG 1 cut(s) 108
SmoI CTYRAG 1 cut(s) 108
Sse9I AATT 3 cut(s) 21, 42, 146
StyI CCWWGG 1 cut(s) 128
TaaI ACNGT 1 cut(s) 520
TasI AATT 3 cut(s) 21, 42, 146
TfiI GAWTC 2 cut(s) 104, 545
Tru1I TTAA 2 cut(s) 20, 378
Tru9I TTAA 2 cut(s) 20, 378
TscAI CASTG 1 cut(s) 542
TseI GCWGC 2 cut(s) 29, 284
TspDTI ATGAA 5 cut(s) 54, 84, 354, 398, 537
TspGWI ACGGA 1 cut(s) 474
TspRI CASTG 1 cut(s) 542
XapI RAATTY 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.