Rh4AG161600

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0025897
Physical Location & Seq
Forward (+)
41032254 .. 41033087
834 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG161600.1

Sequence Viewer

Length: 834 bp
ATGTTACAAGTTGTTGAGAGAAATGGAACAGGAAGGCCAAAACAAGTTAAGATGCGAGAGCTTGCTTTGTCGATAGCCGAGAAGGAAAAGTTTGGTCTTGTATACGACGGGAGACAAGTAATGGAAGATATCTCAACCCGTCGCTTGTCAATTCACAAAATGGACGGAGAAATTAAATCATGGCCGGGTATGTCAAAGATTCGTTCTCTTCTTGTATTTGCCACTGACATGTCCTCATTGTATTTCAATGCACTGGTTTCTCAATTCAAATTGTTGAGGACTCTAGACTTGGAGGATGTCCAAATTGATAAACTGCCGGGTGAAATTGTTTACTTGTTCAACTTGAGATATTTAAATTTGATGGGCACTTTAATCAAGGAACTGCTAGAGTCCATAGGGCGTCTCCGCAACCTTCAATTTCTGAACCTGTGGTGTACTAAGATAGAGTCACTTCCACCAGGAATTGCAAAGTTGCTGAATCTGCGCCATCTAATTATGTATCGCTTCACTGGAAACCGTCTGGACTTCACTGGAAACCGTCTGGCCTTCAAACTTGCGAGTGGGACAAGAGCGCCATCAAATATTTGCAAGTTGCAGAAATTGCAATGTTTATCAATTATTGAATCAGAAGGGGACACTGGTAGACTTATCGGCAACATGACTCAACTTAGGAGAATCGGCATATCGAATGTGAAAGAAAGAGATGAGATTGACCTTTGGGTCTCAATTGAAAAGTTGAATCCGCTTCAATGTTTGATTTTAATGGCAAGTAATGAAGAGGAAATTCTTCCGGTGAAAGCACAATGTTCTTCCCCCTTCAAAAGCTTTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

277

Amino Acids

31.66

Weight (kDa)

9.36

Isoelectric Point (pI)

52.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 66 - 257 5.3e-24 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025897)

Species Orthologous Gene IDs
rosa_rugosa Rorug01G0218000 Rorug05G0042300
rosa_samantha Rh4AG161600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 719
AccI GTMKAC 2 cut(s) 102, 643
AciI CCGC 2 cut(s) 406, 743
AcoI YGGCCR 1 cut(s) 182
AcsI RAATTY 2 cut(s) 355, 783
AcyI GRCGYC 1 cut(s) 400
AfaI GTAC 1 cut(s) 436
AflIII ACRYGT 1 cut(s) 228
AjnI CCWGG 1 cut(s) 457
AluBI AGCT 2 cut(s) 61, 825
AluI AGCT 2 cut(s) 61, 825
Alw26I GTCTC 3 cut(s) 106, 407, 727
AoxI GGCC 3 cut(s) 35, 182, 543
ApoI RAATTY 2 cut(s) 355, 783
Asp700I GAANNNNTTC 1 cut(s) 786
AspLEI GCGC 2 cut(s) 486, 574
AsuC2I CCSGG 2 cut(s) 186, 318
AsuHPI GGTGA 2 cut(s) 332, 805
BaeGI GKGCMC 1 cut(s) 368
BccI CCATC 3 cut(s) 355, 495, 583
BciT130I CCWGG 1 cut(s) 459
BcnI CCSGG 2 cut(s) 186, 318
BcoDI GTCTC 3 cut(s) 106, 407, 727
BfaI CTAG 2 cut(s) 284, 386
BfoI RGCGCY 1 cut(s) 575
Bme1390I CCNGG 3 cut(s) 186, 318, 459
BmrFI CCNGG 3 cut(s) 186, 318, 459
BmsI GCATC 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 364
BpuMI CCSGG 2 cut(s) 186, 318
BsaHI GRCGYC 1 cut(s) 400
BsaI GGTCTC 1 cut(s) 727
BsaWI WCCGGW 1 cut(s) 790
Bse1I ACTGG 4 cut(s) 258, 514, 535, 643
Bse3DI GCAATG 1 cut(s) 611
BseBI CCWGG 1 cut(s) 459
BseGI GGATG 1 cut(s) 301
BseMI GCAATG 1 cut(s) 611
BseNI ACTGG 4 cut(s) 258, 514, 535, 643
BseSI GKGCMC 1 cut(s) 368
BshFI GGCC 3 cut(s) 37, 184, 545
BsiSI CCGG 3 cut(s) 185, 317, 791
BslFI GGGAC 2 cut(s) 577, 647
BsmAI GTCTC 3 cut(s) 106, 407, 727
BsmBI CGTCTC 1 cut(s) 407
BsmFI GGGAC 2 cut(s) 577, 647
BsnI GGCC 3 cut(s) 37, 184, 545
Bso31I GGTCTC 1 cut(s) 727
Bsp1286I GDGCHC 1 cut(s) 368
BspACI CCGC 2 cut(s) 406, 743
BspANI GGCC 3 cut(s) 37, 184, 545
BspTNI GGTCTC 1 cut(s) 727
BsrDI GCAATG 1 cut(s) 611
BsrI ACTGG 4 cut(s) 258, 514, 535, 643
BssNAI GTATAC 1 cut(s) 103
BssNI GRCGYC 1 cut(s) 400
Bst1107I GTATAC 1 cut(s) 103
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 2 cut(s) 518, 539
Bst6I CTCTTC 2 cut(s) 213, 771
BstACI GRCGYC 1 cut(s) 400
BstAPI GCANNNNNTGC 1 cut(s) 601
BstC8I GCNNGC 1 cut(s) 63
BstDEI CTNAG 2 cut(s) 438, 668
BstF5I GGATG 1 cut(s) 301
BstH2I RGCGCY 1 cut(s) 575
BstHHI GCGC 2 cut(s) 486, 574
BstMAI GTCTC 3 cut(s) 106, 407, 727
BstMWI GCNNNNNNNGC 2 cut(s) 481, 601
BstNI CCWGG 1 cut(s) 459
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 3 cut(s) 184, 316, 457
BstSLI GKGCMC 1 cut(s) 368
BstZ17I GTATAC 1 cut(s) 103
BsuRI GGCC 3 cut(s) 37, 184, 545
BtsCI GGATG 1 cut(s) 301
BtsIMutI CAGTG 5 cut(s) 222, 251, 507, 528, 636
Cac8I GCNNGC 1 cut(s) 63
CfoI GCGC 2 cut(s) 486, 574
CseI GACGC 1 cut(s) 389
Csp6I GTAC 1 cut(s) 435
CviAII CATG 3 cut(s) 180, 229, 658
CviJI RGCY 6 cut(s) 37, 61, 77, 184, 545, 825
CviKI_1 RGCY 6 cut(s) 37, 61, 77, 184, 545, 825
CviQI GTAC 1 cut(s) 435
DdeI CTNAG 2 cut(s) 438, 668
DraI TTTAAA 1 cut(s) 354
DrdI GACNNNNNNGTC 1 cut(s) 719
DseDI GACNNNNNNGTC 1 cut(s) 719
EaeI YGGCCR 1 cut(s) 182
Eam1104I CTCTTC 2 cut(s) 213, 771
EarI CTCTTC 2 cut(s) 213, 771
Eco31I GGTCTC 1 cut(s) 727
Eco32I GATATC 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 457
EcoRV GATATC 1 cut(s) 130
Esp3I CGTCTC 1 cut(s) 407
FaeI CATG 3 cut(s) 183, 232, 661
FaiI YATR 8 cut(s) 103, 181, 191, 230, 395, 497, 659, 683
FaqI GGGAC 2 cut(s) 577, 647
FatI CATG 3 cut(s) 179, 228, 657
FblI GTMKAC 2 cut(s) 102, 643
FokI GGATG 1 cut(s) 308
FspBI CTAG 2 cut(s) 284, 386
GlaI GCGC 2 cut(s) 485, 573
HaeII RGCGCY 1 cut(s) 575
HaeIII GGCC 3 cut(s) 37, 184, 545
HapII CCGG 3 cut(s) 185, 317, 791
HgaI GACGC 1 cut(s) 389
HhaI GCGC 2 cut(s) 486, 574
Hin1I GRCGYC 1 cut(s) 400
Hin1II CATG 3 cut(s) 183, 232, 661
Hin6I GCGC 2 cut(s) 484, 572
HinP1I GCGC 2 cut(s) 484, 572
HindIII AAGCTT 1 cut(s) 823
HinfI GANTC 9 cut(s) 199, 280, 389, 446, 478, 623, 661, 675, 739
HpaII CCGG 3 cut(s) 185, 317, 791
HphI GGTGA 2 cut(s) 332, 805
Hpy166II GTNNAC 4 cut(s) 103, 331, 435, 644
Hpy188I TCNGA 2 cut(s) 423, 628
Hpy188III TCNNGA 2 cut(s) 284, 521
Hpy8I GTNNAC 4 cut(s) 103, 331, 435, 644
Hpy99I CGWCG 2 cut(s) 110, 144
HpyAV CCTTC 6 cut(s) 27, 76, 422, 556, 623, 826
HpyCH4III ACNGT 2 cut(s) 518, 539
HpyCH4V TGCA 5 cut(s) 251, 467, 588, 595, 604
HpyF10VI GCNNNNNNNGC 2 cut(s) 481, 601
HpyF3I CTNAG 2 cut(s) 438, 668
Hsp92I GRCGYC 1 cut(s) 400
Hsp92II CATG 3 cut(s) 183, 232, 661
HspAI GCGC 2 cut(s) 484, 572
LweI GCATC 1 cut(s) 42
MaeI CTAG 2 cut(s) 284, 386
MaeIII GTNAC 2 cut(s) 3, 447
MboII GAAGA 5 cut(s) 137, 200, 779, 788, 801
MfeI CAATTG 1 cut(s) 726
MhlI GDGCHC 1 cut(s) 368
MlyI GAGTC 4 cut(s) 274, 398, 455, 655
MnlI CCTC 4 cut(s) 244, 270, 286, 772
MroXI GAANNNNTTC 1 cut(s) 786
MseI TTAA 5 cut(s) 48, 174, 353, 371, 761
MslI CAYNNNNRTG 1 cut(s) 227
MspI CCGG 3 cut(s) 185, 317, 791
MspR9I CCNGG 3 cut(s) 186, 318, 459
MunI CAATTG 1 cut(s) 726
MvaI CCWGG 1 cut(s) 459
MwoI GCNNNNNNNGC 2 cut(s) 481, 601
NciI CCSGG 2 cut(s) 186, 318
NlaIII CATG 3 cut(s) 183, 232, 661
NmeAIII GCCGAG 1 cut(s) 103
NmuCI GTSAC 1 cut(s) 447
NspI RCATGY 1 cut(s) 232
PciI ACATGT 1 cut(s) 228
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 5 cut(s) 199, 478, 623, 675, 739
PleI GAGTC 4 cut(s) 274, 397, 454, 655
PpsI GAGTC 4 cut(s) 274, 397, 454, 655
PscI ACATGT 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 457
PspGI CCWGG 1 cut(s) 457
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
RseI CAYNNNNRTG 1 cut(s) 227
SaqAI TTAA 5 cut(s) 48, 174, 353, 371, 761
SchI GAGTC 4 cut(s) 274, 398, 455, 655
ScrFI CCNGG 3 cut(s) 186, 318, 459
SduI GDGCHC 1 cut(s) 368
SetI ASST 5 cut(s) 63, 414, 429, 717, 827
SfaNI GCATC 1 cut(s) 42
SmiI ATTTAAAT 1 cut(s) 354
SmiMI CAYNNNNRTG 1 cut(s) 227
SmlI CTYRAG 1 cut(s) 343
SmoI CTYRAG 1 cut(s) 343
SsiI CCGC 2 cut(s) 406, 743
SspI AATATT 1 cut(s) 583
SspMI CTAG 2 cut(s) 284, 386
StyD4I CCNGG 3 cut(s) 184, 316, 457
SwaI ATTTAAAT 1 cut(s) 354
TaaI ACNGT 2 cut(s) 518, 539
TaqI TCGA 2 cut(s) 71, 686
TatI WGTACW 1 cut(s) 434
TfiI GAWTC 5 cut(s) 199, 478, 623, 675, 739
Tru1I TTAA 5 cut(s) 48, 174, 353, 371, 761
Tru9I TTAA 5 cut(s) 48, 174, 353, 371, 761
TscAI CASTG 5 cut(s) 229, 258, 514, 535, 643
TseFI GTSAC 1 cut(s) 447
Tsp45I GTSAC 1 cut(s) 447
TspDTI ATGAA 1 cut(s) 789
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 5 cut(s) 229, 258, 514, 535, 643
XapI RAATTY 2 cut(s) 355, 783
XbaI TCTAGA 1 cut(s) 283
XceI RCATGY 1 cut(s) 232
XmiI GTMKAC 2 cut(s) 102, 643
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 2 cut(s) 284, 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.