Rorug05G0042300

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000961
Physical Location & Seq
Reverse (-)
3673936 .. 3675523
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0042300.1

Sequence Viewer

Length: 1089 bp
ATGGGAAGAGCACCGTGTTGTGACAAAAACGGCCTGAAAAAAGGTCCATGGACGCCGGAGGAAGATCAGAAGCTCGTTGATTATATCCAGAAACACGGTTATGGCAACTGGAGGACGCTTCCAAAGAATGCAGGACTGCAAAGATGTGGAAAGAGCTGCCGTCTCCGGTGGACCAACTATCTGCGGCCTGATATCAAACGAGGAAGATTTTCTTTTGAAGAGGAGGAGACAATTGTTCAACTGCATAGCATTTTGGGCAACAAGTGGTCTGCTATTGCTGCTCGTTTGCCTGGAAGAACCGACAATGAAATCAAGAACTACTGGAACACCCACATTAGGAAGAGGCTTCTCCGAATGGGAATCGATCCGGTTACTCACAGTCCTCGGCTTGATCTGCTTGACTTCTCTTCCATTCTATACAACTCTTCTCATCATCACCCTCAAGTAAACTTTTCAAGGTTGCTTGGGATGCAACAAATGGGACTCAACCCTGAGCTACTAAGGCTAGCCACTTCTCTCCTATCATCTCAGAGAGAAAGAAATTCAAACTTCTTCATTCAAAATGACCAGCAGCAGCAGCTTTGCAGTCCCCAAAATGATCAAATCCCAGTACAGCTAGTCCAATCTAGCCAGCTCCAAGAACCCTTTCAAGAAAACGTTCCAGTTTGCGCCACATTGAACACCGGTGATATTCCATTTCCTCATGAAGTACAACCACCAACCATTACTGATTTCAGTTCCCAAAATTGCCAAATAAGTGAGTGGCAAAGTAATGGCGAGGCCCCTTCAAATTTTACTGAAAACCAATATGTTCAATTACCAAGCTTGACATATTATGATGGGTCGTCGGATCACCAGATCGTTAGCGATGTTTCACCCGAAACTTCGAATTTGTACTCCTACAACAGCAACAACCAGAATTTCAACTTCACTTCGGTGTTTTCAACGCCTTGCTCAAGCCCAACGCCGTTGAACTCAAACTCGACTACATACTTCAACAGCGGCACAGAGGATGAACGTGAGAGCTACTGTAGCAACATGTTGAAGTTTGACATCCCAAACATTTTGGATGATTTCATTCATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

362

Amino Acids

41.43

Weight (kDa)

5.81

Isoelectric Point (pI)

47.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 1.1e-18 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 1.9e-13 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 67 - 111 3.3e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 114 9.6e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 184, 1002
AclI AACGTT 1 cut(s) 657
AclWI GGATC 2 cut(s) 359, 858
AcsI RAATTY 4 cut(s) 541, 790, 889, 919
AcyI GRCGYC 1 cut(s) 53
AfaI GTAC 3 cut(s) 612, 711, 896
AfiI CCNNNNNNNGG 1 cut(s) 336
AflIII ACRYGT 1 cut(s) 1038
AgeI ACCGGT 1 cut(s) 683
AjnI CCWGG 1 cut(s) 289
AjuI GAANNNNNNNTTGG 2 cut(s) 630, 662
AleI CACNNNNGTG 1 cut(s) 935
AluBI AGCT 8 cut(s) 73, 156, 496, 580, 616, 634, 825, 1026
AluI AGCT 8 cut(s) 73, 156, 496, 580, 616, 634, 825, 1026
Alw21I GWGCWC 1 cut(s) 13
Alw26I GTCTC 2 cut(s) 167, 221
AlwI GGATC 2 cut(s) 359, 858
AoxI GGCC 3 cut(s) 31, 185, 780
ApeKI GCWGC 5 cut(s) 156, 278, 571, 574, 577
ApoI RAATTY 4 cut(s) 541, 790, 889, 919
AsiGI ACCGGT 1 cut(s) 683
Asp700I GAANNNNTTC 3 cut(s) 208, 645, 657
AspLEI GCGC 1 cut(s) 671
AspS9I GGNCC 3 cut(s) 44, 171, 781
AsuHPI GGTGA 4 cut(s) 428, 698, 845, 867
AsuII TTCGAA 1 cut(s) 887
AsuNHI GCTAGC 1 cut(s) 505
AvaII GGWCC 2 cut(s) 44, 171
Bbv12I GWGCWC 1 cut(s) 13
BbvI GCAGC 5 cut(s) 143, 265, 583, 586, 589
BccI CCATC 1 cut(s) 833
BceAI ACGGC 3 cut(s) 46, 144, 952
BciT130I CCWGG 1 cut(s) 291
BclI TGATCA 1 cut(s) 598
BcoDI GTCTC 2 cut(s) 167, 221
BfaI CTAG 3 cut(s) 506, 617, 627
BfmI CTRYAG 1 cut(s) 1030
BisI GCNGC 7 cut(s) 157, 185, 279, 572, 575, 578, 1003
BlsI GCNGC 7 cut(s) 158, 186, 280, 573, 576, 579, 1004
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 2 cut(s) 44, 171
BmgT120I GGNCC 3 cut(s) 44, 171, 781
BmiI GGNNCC 1 cut(s) 783
BmrFI CCNGG 1 cut(s) 291
BmrI ACTGGG 1 cut(s) 602
BmsI GCATC 1 cut(s) 459
BmtI GCTAGC 1 cut(s) 509
BmuI ACTGGG 1 cut(s) 602
BpmI CTGGAG 1 cut(s) 130
Bpu10I CCTNAGC 1 cut(s) 492
Bpu14I TTCGAA 1 cut(s) 887
BpuEI CTTGAG 2 cut(s) 426, 940
Bsa29I ATCGAT 1 cut(s) 363
BsaHI GRCGYC 1 cut(s) 53
BsaJI CCNNGG 2 cut(s) 47, 383
BsaWI WCCGGW 3 cut(s) 165, 367, 683
Bsc4I CCNNNNNNNGG 1 cut(s) 336
Bse118I RCCGGY 1 cut(s) 683
Bse1I ACTGG 4 cut(s) 113, 326, 608, 662
BseBI CCWGG 1 cut(s) 291
BseCI ATCGAT 1 cut(s) 363
BseDI CCNNGG 2 cut(s) 47, 383
BseGI GGATG 4 cut(s) 474, 1018, 1053, 1075
BseLI CCNNNNNNNGG 1 cut(s) 336
BseMII CTCAG 2 cut(s) 483, 542
BseNI ACTGG 4 cut(s) 113, 326, 608, 662
BseRI GAGGAG 2 cut(s) 236, 239
BseXI GCAGC 5 cut(s) 143, 265, 583, 586, 589
BshFI GGCC 3 cut(s) 33, 187, 782
BshTI ACCGGT 1 cut(s) 683
BshVI ATCGAT 1 cut(s) 363
BsiHKAI GWGCWC 1 cut(s) 13
BsiSI CCGG 4 cut(s) 56, 166, 368, 684
BslFI GGGAC 2 cut(s) 495, 573
BslI CCNNNNNNNGG 1 cut(s) 336
BsmAI GTCTC 2 cut(s) 167, 221
BsmBI CGTCTC 1 cut(s) 167
BsmFI GGGAC 2 cut(s) 495, 573
BsmI GAATGC 1 cut(s) 133
BsnI GGCC 3 cut(s) 33, 187, 782
Bsp119I TTCGAA 1 cut(s) 887
Bsp1286I GDGCHC 1 cut(s) 13
Bsp143I GATC 6 cut(s) 64, 364, 391, 598, 850, 858
Bsp19I CCATGG 1 cut(s) 47
BspACI CCGC 2 cut(s) 184, 1002
BspANI GGCC 3 cut(s) 33, 187, 782
BspCNI CTCAG 2 cut(s) 484, 541
BspDI ATCGAT 1 cut(s) 363
BspHI TCATGA 1 cut(s) 703
BspLI GGNNCC 1 cut(s) 783
BspOI GCTAGC 1 cut(s) 509
BspPI GGATC 2 cut(s) 359, 858
BspT104I TTCGAA 1 cut(s) 887
BsrFI RCCGGY 1 cut(s) 683
BsrI ACTGG 4 cut(s) 113, 326, 608, 662
BssAI RCCGGY 1 cut(s) 683
BssECI CCNNGG 2 cut(s) 47, 383
BssMI GATC 6 cut(s) 64, 364, 391, 598, 850, 858
BssNI GRCGYC 1 cut(s) 53
BssT1I CCWWGG 1 cut(s) 47
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 4 cut(s) 15, 98, 380, 1031
Bst6I CTCTTC 4 cut(s) 213, 335, 412, 430
BstACI GRCGYC 1 cut(s) 53
BstBI TTCGAA 1 cut(s) 887
BstC8I GCNNGC 2 cut(s) 507, 632
BstDEI CTNAG 3 cut(s) 492, 500, 528
BstDSI CCRYGG 1 cut(s) 47
BstF5I GGATG 4 cut(s) 474, 1018, 1053, 1075
BstHHI GCGC 1 cut(s) 671
BstKTI GATC 6 cut(s) 67, 367, 394, 601, 853, 861
BstMAI GTCTC 2 cut(s) 167, 221
BstMBI GATC 6 cut(s) 64, 364, 391, 598, 850, 858
BstMWI GCNNNNNNNGC 7 cut(s) 255, 278, 394, 469, 502, 577, 1032
BstNI CCWGG 1 cut(s) 291
BstNSI RCATGY 1 cut(s) 1042
BstSCI CCNGG 1 cut(s) 289
BstSFI CTRYAG 1 cut(s) 1030
BstV1I GCAGC 5 cut(s) 143, 265, 583, 586, 589
Bsu15I ATCGAT 1 cut(s) 363
BsuRI GGCC 3 cut(s) 33, 187, 782
BsuTUI ATCGAT 1 cut(s) 363
BtgI CCRYGG 1 cut(s) 47
BtgZI GCGATG 1 cut(s) 882
BtsCI GGATG 4 cut(s) 474, 1018, 1053, 1075
Cac8I GCNNGC 2 cut(s) 507, 632
CciI TCATGA 1 cut(s) 703
CfoI GCGC 1 cut(s) 671
Cfr10I RCCGGY 1 cut(s) 683
Cfr13I GGNCC 3 cut(s) 44, 171, 781
ClaI ATCGAT 1 cut(s) 363
CseI GACGC 2 cut(s) 61, 124
Csp6I GTAC 3 cut(s) 611, 710, 895
CspAI ACCGGT 1 cut(s) 683
CviAII CATG 4 cut(s) 48, 704, 1039, 1082
CviQI GTAC 3 cut(s) 611, 710, 895
DdeI CTNAG 3 cut(s) 492, 500, 528
DpnI GATC 6 cut(s) 66, 366, 393, 600, 852, 860
DpnII GATC 6 cut(s) 64, 364, 391, 598, 850, 858
Eam1104I CTCTTC 4 cut(s) 213, 335, 412, 430
EarI CTCTTC 4 cut(s) 213, 335, 412, 430
Eco130I CCWWGG 1 cut(s) 47
Eco32I GATATC 1 cut(s) 193
Eco47I GGWCC 2 cut(s) 44, 171
EcoO109I RGGNCCY 1 cut(s) 781
EcoRII CCWGG 1 cut(s) 289
EcoRV GATATC 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 47
ErhI CCWWGG 1 cut(s) 47
Esp3I CGTCTC 1 cut(s) 167
FaeI CATG 4 cut(s) 51, 707, 1042, 1085
FalI AAGNNNNNCTT 2 cut(s) 196, 228
FaqI GGGAC 2 cut(s) 495, 573
FatI CATG 4 cut(s) 47, 703, 1038, 1081
FbaI TGATCA 1 cut(s) 598
Fnu4HI GCNGC 7 cut(s) 157, 185, 279, 572, 575, 578, 1003
FokI GGATG 4 cut(s) 481, 1025, 1040, 1082
Fsp4HI GCNGC 7 cut(s) 157, 185, 279, 572, 575, 578, 1003
FspBI CTAG 3 cut(s) 506, 617, 627
GlaI GCGC 1 cut(s) 670
GluI GCNGC 7 cut(s) 157, 185, 279, 572, 575, 578, 1003
GsuI CTGGAG 1 cut(s) 130
HaeIII GGCC 3 cut(s) 33, 187, 782
HapII CCGG 4 cut(s) 56, 166, 368, 684
HgaI GACGC 2 cut(s) 61, 124
HhaI GCGC 1 cut(s) 671
Hin1I GRCGYC 1 cut(s) 53
Hin1II CATG 4 cut(s) 51, 707, 1042, 1085
Hin6I GCGC 1 cut(s) 669
HinP1I GCGC 1 cut(s) 669
HindIII AAGCTT 1 cut(s) 823
HinfI GANTC 2 cut(s) 360, 483
HpaII CCGG 4 cut(s) 56, 166, 368, 684
HphI GGTGA 4 cut(s) 428, 698, 845, 867
Hpy166II GTNNAC 2 cut(s) 171, 448
Hpy188I TCNGA 4 cut(s) 69, 353, 531, 850
Hpy188III TCNNGA 4 cut(s) 88, 313, 650, 704
Hpy8I GTNNAC 2 cut(s) 171, 448
Hpy99I CGWCG 1 cut(s) 850
HpyAV CCTTC 1 cut(s) 795
HpyCH4III ACNGT 4 cut(s) 15, 98, 380, 1031
HpyCH4IV ACGT 2 cut(s) 657, 1018
HpyCH4V TGCA 5 cut(s) 131, 139, 244, 472, 585
HpyF10VI GCNNNNNNNGC 7 cut(s) 255, 278, 394, 469, 502, 577, 1032
HpyF3I CTNAG 3 cut(s) 492, 500, 528
HpySE526I ACGT 2 cut(s) 657, 1018
Hsp92I GRCGYC 1 cut(s) 53
Hsp92II CATG 4 cut(s) 51, 707, 1042, 1085
HspAI GCGC 1 cut(s) 669
Ksp22I TGATCA 1 cut(s) 598
Kzo9I GATC 6 cut(s) 64, 364, 391, 598, 850, 858
LmnI GCTCC 1 cut(s) 639
Lsp1109I GCAGC 5 cut(s) 143, 265, 583, 586, 589
LweI GCATC 1 cut(s) 459
MaeI CTAG 3 cut(s) 506, 617, 627
MaeII ACGT 2 cut(s) 657, 1018
MaeIII GTNAC 2 cut(s) 20, 370
MalI GATC 6 cut(s) 66, 366, 393, 600, 852, 860
MboI GATC 6 cut(s) 64, 364, 391, 598, 850, 858
MboII GAAGA 9 cut(s) 18, 74, 216, 230, 306, 352, 399, 417, 544
MfeI CAATTG 1 cut(s) 231
MhlI GDGCHC 1 cut(s) 13
MluCI AATT 7 cut(s) 231, 541, 745, 790, 815, 889, 919
MlyI GAGTC 1 cut(s) 477
MmeI TCCRAC 1 cut(s) 828
MroXI GAANNNNTTC 3 cut(s) 208, 645, 657
MslI CAYNNNNRTG 3 cut(s) 99, 935, 1082
MspA1I CMGCKG 1 cut(s) 1002
MspI CCGG 4 cut(s) 56, 166, 368, 684
MspR9I CCNGG 1 cut(s) 291
MunI CAATTG 1 cut(s) 231
Mva1269I GAATGC 1 cut(s) 133
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 7 cut(s) 255, 278, 394, 469, 502, 577, 1032
NcoI CCATGG 1 cut(s) 47
NdeII GATC 6 cut(s) 64, 364, 391, 598, 850, 858
NheI GCTAGC 1 cut(s) 505
NlaIII CATG 4 cut(s) 51, 707, 1042, 1085
NlaIV GGNNCC 1 cut(s) 783
NmeAIII GCCGAG 1 cut(s) 364
NmuCI GTSAC 1 cut(s) 20
NspI RCATGY 1 cut(s) 1042
NspV TTCGAA 1 cut(s) 887
OliI CACNNNNGTG 1 cut(s) 935
PagI TCATGA 1 cut(s) 703
PciI ACATGT 1 cut(s) 1038
PctI GAATGC 1 cut(s) 133
PdmI GAANNNNTTC 3 cut(s) 208, 645, 657
PfeI GAWTC 1 cut(s) 360
PinAI ACCGGT 1 cut(s) 683
PkrI GCNGC 7 cut(s) 158, 186, 280, 573, 576, 579, 1004
PleI GAGTC 1 cut(s) 477
PpsI GAGTC 1 cut(s) 477
PscI ACATGT 1 cut(s) 1038
Psp1406I AACGTT 1 cut(s) 657
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 783
PspPI GGNCC 3 cut(s) 44, 171, 781
RsaI GTAC 3 cut(s) 612, 711, 896
RsaNI GTAC 3 cut(s) 611, 710, 895
RseI CAYNNNNRTG 3 cut(s) 99, 935, 1082
SatI GCNGC 7 cut(s) 157, 185, 279, 572, 575, 578, 1003
Sau3AI GATC 6 cut(s) 64, 364, 391, 598, 850, 858
Sau96I GGNCC 3 cut(s) 44, 171, 781
SchI GAGTC 1 cut(s) 477
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 1 cut(s) 13
SfaNI GCATC 1 cut(s) 459
SfcI CTRYAG 1 cut(s) 1030
SfuI TTCGAA 1 cut(s) 887
SgrAI CRCCGGYG 1 cut(s) 683
SinI GGWCC 2 cut(s) 44, 171
SmiMI CAYNNNNRTG 3 cut(s) 99, 935, 1082
SmlI CTYRAG 2 cut(s) 441, 955
SmoI CTYRAG 2 cut(s) 441, 955
Sse9I AATT 7 cut(s) 231, 541, 745, 790, 815, 889, 919
SsiI CCGC 2 cut(s) 184, 1002
SspMI CTAG 3 cut(s) 506, 617, 627
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 4 cut(s) 15, 98, 380, 1031
TaiI ACGT 2 cut(s) 660, 1021
TaqI TCGA 3 cut(s) 363, 887, 983
TasI AATT 7 cut(s) 231, 541, 745, 790, 815, 889, 919
TatI WGTACW 3 cut(s) 610, 709, 894
TauI GCSGC 2 cut(s) 187, 1005
TfiI GAWTC 1 cut(s) 360
TseFI GTSAC 1 cut(s) 20
TseI GCWGC 5 cut(s) 156, 278, 571, 574, 577
Tsp45I GTSAC 1 cut(s) 20
TspDTI ATGAA 6 cut(s) 321, 544, 720, 1029, 1066, 1070
VpaK11BI GGWCC 2 cut(s) 44, 171
XapI RAATTY 4 cut(s) 541, 790, 889, 919
XceI RCATGY 1 cut(s) 1042
XmnI GAANNNNTTC 3 cut(s) 208, 645, 657
XspI CTAG 3 cut(s) 506, 617, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.