Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0000961

Physical Location & Seq

Genomic Coordinates Forward (+)

71104551 .. 71107643

Gene Structure 3093 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0588900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGTCACCTGCTCTCTTTCTATCTCTCCCCCTCTCTTCAGTCTGTAAAACCCAGGAAATTTCCAAGGACACCTACCTCAACACCAACAGAGAAACCCCATGTCACAAGTCTCACTCAAACTTGAAATCCACTCGAAGATCTCTATTAAATTCTGCCACTCTTGGACTCATAGTGCCTGGACCCCTCTCTGTTTCTCACCCTGCAAAAGCTGAGCCTGAGCCAGAGAGCCCGATTGCATCAACTTCAAGCAGAATGTCATACTCAAGATTCTTGCAGTACTTGGATGAAGGAGTTGTGAGGAAGGTAGACCTGTTTGATCAGAATGGAACTGTCGCGATCGCCGAGATCTTCAACCCCACATTAGAGAAGCTCCAGAGAGTCAGAGTTCAGCTACCTGGATTGCCACCAGAGTTGCTGAGGAAACTAGAAGAGAAGAACATAGACTTTGCAGCTCATCCAAGGGAAGTGGATTGGTGGCCTGCAGTACTGGACTTGTTGGGGAATTTTGCTTTTCCATTGATACTTCTTGGGATATTGCTTTTGAGGAGTTCATCACCAAACATACAAGGAGGCCCCAACTTACCTTTTGGACTTGGAAGGAGCAAAGCCAAATTCCAAATGGAACCGAATACCGGGGTAACATTTGATGATGTGGCTGGGGTCGATGAAGCAAAGCAAGATTTCCAAGAGATTGTTGAATTTCTCAAAACTCCAGAGAAGTTTGCTGCCGTTGGTGCAAGAATTCCCAAAGGGGTTCTTTTAGTAGGGCCACCAGGGACCGGAAAGACATTGCTGGGTAAGGCCATTGCAGGAGAGGCAGCGGTGCCTTTCTTTTCTCTGTCAGGGTCAGAGTTCATTGAGATGTTTGTAGGAGTAGGAGCTTCTAGAGTAAGAGACTTGTTCAACAAGGCAAAAGAGAATTCGCCGTGCTTGGTTTTCATTGATGAAATTGATGCTGTTGGGAGGCAAAGAGGGACAGGAATTGGTGGAGGAAATGATGAGAGGGAGCAGACATTGAATCAGCTGCTTACTGAAATGGATGGATTTAGTGGGAACAGTGGAGTAATTGTTATTGCTGCTACTAATCGCCCTGAAATTCTTGATTCTGCTTTGCTTAGGCCCGGAAGGTTTGACAGACAAGTCACAGTTGGATTGCCAGATATTAAGGGAAGAAACGATATATTAAAGGTTCATAGTAACAACAAGAAGCTTGATAAGAGTGTTTCTCTTGATATTATTGCTATGAGAACTCCTGGCTTCAGCGGTGCAGATCTTGCAAACCTCATGAATGAAGCTGCGATTCTTGCTGGCAGGAGAGGCAAAGATAAGATAACAATGAAAGAAATAGATGATTCGATTGATCGAATTGTTGCAGGGATGGAAGGCACCAAAATGACTGATGGAAAGAACAAAACTCTTGTGGCTTACCATGAAGTAGGCCACGCCATTTGCGCAACATTGACTCCAGGCCATGATCCAGTGCAGAAGGTCTCTCTTATTCCCCGAGGCCAAGCTCGAGGTCTCACATGGTTCTTACCGGGTGAAGATCCCGCTCTTATTTCTAAGCAACAGCTGTTTGCTAGAATAGTTGGAGGTCTAGGAGGTAGAGCAGCAGAAGAAGTAATTTTTGGAGAGCCAGAAATAACAACCGGTGCAGCAGGAGACTTGCAGCAGGTCACACAAATAGCAAGACAGATGGTAACAGTGTTTGGCATGTCCGAGATTGGACCATGGGCATTGACAGACCCAGCGGCCCAAAGCAGTGATGTAGTGTTGAGGATGCTAGCAAGGAACACTATGTCCGAAAAACTTGCAGAGGATATTGACTTGTCAGTGAGACATATAGTTGAAAGTGCATATGAGGTTGCAAAGAAGCATATAAGGAATAACCGGGAAGCCATTGATAATTTAGTAGATGTGCTATTAGAAAAGGAGATCCTCACTACTGGAGATGAGTTCAGGGCAATTCTGTCTGAATTTACTGATACTTCTATGGTAGAGCCGGATAGAAACTCGGTCAGGGAGATGATTGAAGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

OPT

Protein Analysis

679

Amino Acids

73.82

Weight (kDa)

5.55

Isoelectric Point (pI)

40.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FtsH_ext	PF06480	80 - 153	1.3e-07	FtsH Extracellular
AAA	PF00004	252 - 384	4.1e-45	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	407 - 450	7.6e-14	AAA+ lid domain
Peptidase_M41	PF01434	465 - 649	1.9e-66	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000961)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27020 AT5G41000 AT5G41000 AT5G41000
fragaria_vesca	FvH4_6g53820
malus_domestica	MD09G1008400.v1.1 MD17G1002400.v1.1
prunus_persica	Prupe.3G197700_v2.0.a1 Prupe.3G308600_v2.0.a1
pyrus_communis	pycom111g00670 pycom17g00700
rosa_chinensis	RchiOBHm_Chr2g0155151 RchiOBHm_Chr2g0175951 RchiOBHm_Chr4g0387931 RchiOBHm_Chr4g0404811 RchiOBHm_Chr6g0247711 RchiOBHm_Chr6g0308411 RchiOBHm_Chr7g0225381
rosa_laevigata	RLG00000001821 RLG00000022368
rosa_multiflora	Rmu_co8150112.1_g000001 Rmu_sc0007419.1_g000008 Rmu_sc0012106.1_g000001 Rmu_sc0018801.1_g000005
rosa_roxburghii	Rroxscaffold_2G00076910 Rroxscaffold_3G00233840 Rroxscaffold_5G00348620 Rroxscaffold_6G00388000 Rroxscaffold_7G00165420
rosa_rugosa	Rorug01G0171700 Rorug02G0588900 Rorug02G0589000 Rorug03G0332100 Rorug03G0332200 Rorug05G0042300 Rorug05G0110200 Rorug07G0229200
rosa_samantha	Rh1AG155500 Rh1CG145800 Rh2AG668100 Rh2BG679600 Rh2CG642400 Rh2DG572100 Rh2DG693100 Rh4AG072500 Rh4AG074300 Rh4BG070600 Rh4CG078900 Rh4DG161200 Rh5AG218500 Rh5BG431200 Rh5BG537600 Rh5CG454500 Rh5CG561600 Rh6AG476100 Rh7AG374200 Rh7BG363900 Rh7CG392400 Rh7DG375100
rosa_wichuraiana	Rw2G054770 Rw3G025300 Rw4G006410 Rw7G031640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	16
AasI	GACNNNNNNGTC	1 cut(s)	1139
Acc16I	TGCGCA	1 cut(s)	1453
Acc36I	ACCTGC	2 cut(s)	16, 1663
AccB1I	GGYRCC	2 cut(s)	823, 1385
AccBSI	CCGCTC	1 cut(s)	1553
AccI	GTMKAC	1 cut(s)	306
AccII	CGCG	1 cut(s)	335
AciI	CCGC	4 cut(s)	821, 1263, 1551, 1751
AclWI	GGATC	3 cut(s)	1469, 1541, 1930
AcsI	RAATTY	9 cut(s)	57, 148, 502, 611, 698, 741, 919, 1095, 1976
AcuI	CTGAAG	2 cut(s)	21, 1243
AfaI	GTAC	2 cut(s)	278, 486
AfiI	CCNNNNNNNGG	2 cut(s)	632, 779
AgeI	ACCGGT	1 cut(s)	1649
AgsI	TTSAA	8 cut(s)	124, 246, 352, 698, 904, 1018, 1850, 2033
AjnI	CCWGG	6 cut(s)	51, 175, 394, 772, 1252, 1465
AjuI	GAANNNNNNNTTGG	2 cut(s)	1611, 1643
AloI	GAACNNNNNNTCC	2 cut(s)	1784, 1816
Alw26I	GTCTC	6 cut(s)	114, 888, 1495, 1526, 1656, 1831
AlwI	GGATC	3 cut(s)	1469, 1541, 1930
Ama87I	CYCGRG	2 cut(s)	1503, 1515
AoxI	GGCC	9 cut(s)	476, 571, 767, 801, 1118, 1438, 1468, 1507, 1752
ApeKI	GCWGC	9 cut(s)	449, 725, 818, 1024, 1076, 1295, 1610, 1655, 1669
ApoI	RAATTY	9 cut(s)	57, 148, 502, 611, 698, 741, 919, 1095, 1976
AsiGI	ACCGGT	1 cut(s)	1649
AsiSI	GCGATCGC	1 cut(s)	339
AspLEI	GCGC	1 cut(s)	1454
AspS9I	GGNCC	7 cut(s)	179, 572, 767, 777, 1119, 1727, 1753
AsuC2I	CCSGG	4 cut(s)	634, 1122, 1539, 1892
AsuHPI	GGTGA	3 cut(s)	188, 546, 1553
AsuNHI	GCTAGC	1 cut(s)	1783
AvaI	CYCGRG	2 cut(s)	1503, 1515
AvaII	GGWCC	3 cut(s)	179, 777, 1727
BanI	GGYRCC	2 cut(s)	823, 1385
BanII	GRGCYC	1 cut(s)	230
BbvCI	CCTCAGC	1 cut(s)	416
BbvI	GCAGC	9 cut(s)	461, 712, 830, 1011, 1063, 1282, 1622, 1667, 1681
BccI	CCATC	4 cut(s)	1034, 1372, 1394, 1690
BceAI	ACGGC	2 cut(s)	713, 910
BcgI	CGANNNNNNTGC	2 cut(s)	1793, 1827
BciT130I	CCWGG	6 cut(s)	53, 177, 396, 774, 1254, 1467
BclI	TGATCA	1 cut(s)	316
BcnI	CCSGG	4 cut(s)	634, 1122, 1539, 1892
BcoDI	GTCTC	6 cut(s)	114, 888, 1495, 1526, 1656, 1831
BfaI	CTAG	6 cut(s)	425, 885, 1581, 1598, 1784, 2038
BfmI	CTRYAG	1 cut(s)	480
BfuAI	ACCTGC	2 cut(s)	16, 1663
BglII	AGATCT	3 cut(s)	137, 345, 1270
BlpI	GCTNAGC	1 cut(s)	210
BmcAI	AGTACT	2 cut(s)	278, 486
Bme18I	GGWCC	3 cut(s)	179, 777, 1727
BmeT110I	CYCGRG	2 cut(s)	1503, 1515
BmgT120I	GGNCC	7 cut(s)	179, 572, 767, 777, 1119, 1727, 1753
BmiI	GGNNCC	6 cut(s)	181, 574, 624, 778, 825, 1387
BmsI	GCATC	3 cut(s)	245, 943, 1770
BmtI	GCTAGC	1 cut(s)	1787
BplI	GAGNNNNNCTC	2 cut(s)	1210, 1242
BpmI	CTGGAG	4 cut(s)	356, 696, 1449, 1968
Bpu10I	CCTNAGC	3 cut(s)	216, 416, 1115
Bpu1102I	GCTNAGC	1 cut(s)	210
BpuEI	CTTGAG	1 cut(s)	247
BpuMI	CCSGG	4 cut(s)	634, 1122, 1539, 1892
BsaI	GGTCTC	2 cut(s)	1495, 1526
BsaJI	CCNNGG	7 cut(s)	51, 63, 458, 633, 773, 1504, 1730
BsaWI	WCCGGW	2 cut(s)	779, 1649
BsaXI	ACNNNNNCTCC	4 cut(s)	1053, 1083, 1447, 1477
Bsc4I	CCNNNNNNNGG	2 cut(s)	632, 779
Bse118I	RCCGGY	1 cut(s)	1649
Bse1I	ACTGG	3 cut(s)	492, 1478, 1951
Bse3DI	GCAATG	2 cut(s)	788, 804
BseBI	CCWGG	6 cut(s)	53, 177, 396, 774, 1254, 1467
BseDI	CCNNGG	7 cut(s)	51, 63, 458, 633, 773, 1504, 1730
BseGI	GGATG	5 cut(s)	289, 454, 1045, 1383, 1785
BseLI	CCNNNNNNNGG	2 cut(s)	632, 779
BseMI	GCAATG	2 cut(s)	788, 804
BseMII	CTCAG	3 cut(s)	201, 207, 407
BseNI	ACTGG	3 cut(s)	492, 1478, 1951
BseRI	GAGGAG	1 cut(s)	559
BseXI	GCAGC	9 cut(s)	461, 712, 830, 1011, 1063, 1282, 1622, 1667, 1681
BseYI	CCCAGC	3 cut(s)	656, 793, 1747
BsgI	GTGCAG	3 cut(s)	1287, 1502, 1674
Bsh1236I	CGCG	1 cut(s)	335
Bsh1285I	CGRYCG	1 cut(s)	339
BshFI	GGCC	9 cut(s)	478, 573, 769, 803, 1120, 1440, 1470, 1509, 1754
BshNI	GGYRCC	2 cut(s)	823, 1385
BshTI	ACCGGT	1 cut(s)	1649
BsiEI	CGRYCG	1 cut(s)	339
BsiHKCI	CYCGRG	2 cut(s)	1503, 1515
BsiSI	CCGG	7 cut(s)	633, 780, 1122, 1538, 1650, 1891, 2003
BslFI	GGGAC	2 cut(s)	790, 988
BslI	CCNNNNNNNGG	2 cut(s)	632, 779
BsmAI	GTCTC	6 cut(s)	114, 888, 1495, 1526, 1656, 1831
BsmFI	GGGAC	2 cut(s)	790, 988
BsnI	GGCC	9 cut(s)	478, 573, 769, 803, 1120, 1440, 1470, 1509, 1754
Bso31I	GGTCTC	2 cut(s)	1495, 1526
BsoBI	CYCGRG	2 cut(s)	1503, 1515
Bsp1286I	GDGCHC	1 cut(s)	230
Bsp143I	GATC	9 cut(s)	137, 316, 336, 345, 1270, 1360, 1474, 1546, 1935
Bsp1720I	GCTNAGC	1 cut(s)	210
Bsp19I	CCATGG	1 cut(s)	1730
Bsp68I	TCGCGA	1 cut(s)	335
BspACI	CCGC	4 cut(s)	821, 1263, 1551, 1751
BspANI	GGCC	9 cut(s)	478, 573, 769, 803, 1120, 1440, 1470, 1509, 1754
BspCNI	CTCAG	3 cut(s)	202, 208, 408
BspFNI	CGCG	1 cut(s)	335
BspHI	TCATGA	1 cut(s)	1284
BspLI	GGNNCC	6 cut(s)	181, 574, 624, 778, 825, 1387
BspMAI	CTGCAG	1 cut(s)	484
BspMI	ACCTGC	2 cut(s)	16, 1663
BspOI	GCTAGC	1 cut(s)	1787
BspPI	GGATC	3 cut(s)	1469, 1541, 1930
BspT107I	GGYRCC	2 cut(s)	823, 1385
BspTNI	GGTCTC	2 cut(s)	1495, 1526
BsrBI	CCGCTC	1 cut(s)	1553
BsrDI	GCAATG	2 cut(s)	788, 804
BsrFI	RCCGGY	1 cut(s)	1649
BsrI	ACTGG	3 cut(s)	492, 1478, 1951
BssAI	RCCGGY	1 cut(s)	1649
BssECI	CCNNGG	7 cut(s)	51, 63, 458, 633, 773, 1504, 1730
BssMI	GATC	9 cut(s)	137, 316, 336, 345, 1270, 1360, 1474, 1546, 1935
BssT1I	CCWWGG	3 cut(s)	63, 458, 1730
Bst2UI	CCWGG	6 cut(s)	53, 177, 396, 774, 1254, 1467
Bst4CI	ACNGT	4 cut(s)	331, 1058, 1147, 1705
Bst6I	CTCTTC	2 cut(s)	40, 423
BstAPI	GCANNNNNTGC	1 cut(s)	1274
BstC8I	GCNNGC	3 cut(s)	480, 1309, 1785
BstDEI	CTNAG	5 cut(s)	210, 216, 416, 1115, 1563
BstDSI	CCRYGG	1 cut(s)	1730
BstF5I	GGATG	5 cut(s)	289, 454, 1045, 1383, 1785
BstFNI	CGCG	1 cut(s)	335
BstHHI	GCGC	1 cut(s)	1454
BstKTI	GATC	9 cut(s)	140, 319, 339, 348, 1273, 1363, 1477, 1549, 1938
BstMAI	GTCTC	6 cut(s)	114, 888, 1495, 1526, 1656, 1831
BstMBI	GATC	9 cut(s)	137, 316, 336, 345, 1270, 1360, 1474, 1546, 1935
BstMCI	CGRYCG	1 cut(s)	339
BstMWI	GCNNNNNNNGC	6 cut(s)	734, 815, 1274, 1304, 1317, 1451
BstNI	CCWGG	6 cut(s)	53, 177, 396, 774, 1254, 1467
BstNSI	RCATGY	1 cut(s)	1717
BstSFI	CTRYAG	1 cut(s)	480
BstUI	CGCG	1 cut(s)	335
BstV1I	GCAGC	9 cut(s)	461, 712, 830, 1011, 1063, 1282, 1622, 1667, 1681
BstX2I	RGATCY	5 cut(s)	137, 345, 1270, 1546, 1935
BstYI	RGATCY	5 cut(s)	137, 345, 1270, 1546, 1935
BsuRI	GGCC	9 cut(s)	478, 573, 769, 803, 1120, 1440, 1470, 1509, 1754
BtgI	CCRYGG	1 cut(s)	1730
BtsCI	GGATG	5 cut(s)	289, 454, 1045, 1383, 1785
BtsI	GCAGTG	1 cut(s)	1768
BtsIMutI	CAGTG	5 cut(s)	1063, 1485, 1710, 1768, 1839
BtuMI	TCGCGA	1 cut(s)	335
BveI	ACCTGC	2 cut(s)	16, 1663
Cac8I	GCNNGC	3 cut(s)	480, 1309, 1785
CciI	TCATGA	1 cut(s)	1284
CfoI	GCGC	1 cut(s)	1454
Cfr10I	RCCGGY	1 cut(s)	1649
Cfr13I	GGNCC	7 cut(s)	179, 572, 767, 777, 1119, 1727, 1753
Csp6I	GTAC	2 cut(s)	277, 485
CspAI	ACCGGT	1 cut(s)	1649
CspCI	CAANNNNNGTGG	6 cut(s)	393, 428, 447, 482, 1430, 1465
CviAII	CATG	7 cut(s)	99, 1285, 1430, 1472, 1527, 1714, 1731
CviQI	GTAC	2 cut(s)	277, 485
DdeI	CTNAG	5 cut(s)	210, 216, 416, 1115, 1563
DpnI	GATC	9 cut(s)	139, 318, 338, 347, 1272, 1362, 1476, 1548, 1937
DpnII	GATC	9 cut(s)	137, 316, 336, 345, 1270, 1360, 1474, 1546, 1935
DrdI	GACNNNNNNGTC	1 cut(s)	1139
DseDI	GACNNNNNNGTC	1 cut(s)	1139
Eam1104I	CTCTTC	2 cut(s)	40, 423
EarI	CTCTTC	2 cut(s)	40, 423
Eco130I	CCWWGG	3 cut(s)	63, 458, 1730
Eco24I	GRGCYC	1 cut(s)	230
Eco31I	GGTCTC	2 cut(s)	1495, 1526
Eco47I	GGWCC	3 cut(s)	179, 777, 1727
Eco57I	CTGAAG	2 cut(s)	21, 1243
Eco88I	CYCGRG	2 cut(s)	1503, 1515
EcoO109I	RGGNCCY	1 cut(s)	572
EcoRI	GAATTC	2 cut(s)	741, 919
EcoRII	CCWGG	6 cut(s)	51, 175, 394, 772, 1252, 1465
EcoT14I	CCWWGG	3 cut(s)	63, 458, 1730
EcoT38I	GRGCYC	1 cut(s)	230
ErhI	CCWWGG	3 cut(s)	63, 458, 1730
FaeI	CATG	7 cut(s)	102, 1288, 1433, 1475, 1530, 1717, 1734
FalI	AAGNNNNNCTT	2 cut(s)	741, 773
FaqI	GGGAC	2 cut(s)	790, 988
FatI	CATG	7 cut(s)	98, 1284, 1429, 1471, 1526, 1713, 1730
FauI	CCCGC	1 cut(s)	1558
FauNDI	CATATG	1 cut(s)	1858
FbaI	TGATCA	1 cut(s)	316
FblI	GTMKAC	1 cut(s)	306
FokI	GGATG	5 cut(s)	296, 441, 1052, 1390, 1792
FriOI	GRGCYC	1 cut(s)	230
FspBI	CTAG	6 cut(s)	425, 885, 1581, 1598, 1784, 2038
FspI	TGCGCA	1 cut(s)	1453
GlaI	GCGC	1 cut(s)	1453
GsaI	CCCAGC	3 cut(s)	660, 797, 1751
GsuI	CTGGAG	4 cut(s)	356, 696, 1449, 1968
HaeIII	GGCC	9 cut(s)	478, 573, 769, 803, 1120, 1440, 1470, 1509, 1754
HapII	CCGG	7 cut(s)	633, 780, 1122, 1538, 1650, 1891, 2003
HhaI	GCGC	1 cut(s)	1454
Hin1II	CATG	7 cut(s)	102, 1288, 1433, 1475, 1530, 1717, 1734
Hin6I	GCGC	1 cut(s)	1452
HinP1I	GCGC	1 cut(s)	1452
HindIII	AAGCTT	1 cut(s)	1208
HinfI	GANTC	8 cut(s)	165, 267, 378, 1018, 1103, 1300, 1352, 1462
HpaII	CCGG	7 cut(s)	633, 780, 1122, 1538, 1650, 1891, 2003
HphI	GGTGA	3 cut(s)	188, 546, 1553
Hpy166II	GTNNAC	1 cut(s)	307
Hpy188I	TCNGA	6 cut(s)	321, 383, 850, 1720, 1804, 1975
Hpy188III	TCNNGA	8 cut(s)	264, 334, 373, 713, 885, 1100, 1229, 1285
Hpy8I	GTNNAC	1 cut(s)	307
HpyAV	CCTTC	6 cut(s)	281, 295, 591, 1119, 1376, 1480
HpyCH4III	ACNGT	4 cut(s)	331, 1058, 1147, 1705
HpyF10VI	GCNNNNNNNGC	6 cut(s)	734, 815, 1274, 1304, 1317, 1451
HpyF3I	CTNAG	5 cut(s)	210, 216, 416, 1115, 1563
Hsp92II	CATG	7 cut(s)	102, 1288, 1433, 1475, 1530, 1717, 1734
HspAI	GCGC	1 cut(s)	1452
Ksp22I	TGATCA	1 cut(s)	316
Kzo9I	GATC	9 cut(s)	137, 316, 336, 345, 1270, 1360, 1474, 1546, 1935
LmnI	GCTCC	4 cut(s)	375, 600, 878, 1006
Lsp1109I	GCAGC	9 cut(s)	461, 712, 830, 1011, 1063, 1282, 1622, 1667, 1681
LweI	GCATC	3 cut(s)	245, 943, 1770
MaeI	CTAG	6 cut(s)	425, 885, 1581, 1598, 1784, 2038
MaeIII	GTNAC	7 cut(s)	3, 101, 637, 1141, 1196, 1675, 1699
MalI	GATC	9 cut(s)	139, 318, 338, 347, 1272, 1362, 1476, 1548, 1937
MbiI	CCGCTC	1 cut(s)	1553
MboI	GATC	9 cut(s)	137, 316, 336, 345, 1270, 1360, 1474, 1546, 1935
MboII	GAAGA	8 cut(s)	27, 147, 340, 440, 445, 1182, 1556, 1628
MflI	RGATCY	5 cut(s)	137, 345, 1270, 1546, 1935
MhlI	GDGCHC	1 cut(s)	230
MlyI	GAGTC	3 cut(s)	159, 387, 1456
MmeI	TCCRAC	2 cut(s)	1129, 1570
MseI	TTAA	3 cut(s)	146, 1164, 1184
MslI	CAYNNNNRTG	2 cut(s)	860, 1391
MspA1I	CMGCKG	5 cut(s)	821, 1024, 1263, 1573, 1751
MspI	CCGG	7 cut(s)	633, 780, 1122, 1538, 1650, 1891, 2003
MvaI	CCWGG	6 cut(s)	53, 177, 396, 774, 1254, 1467
MvnI	CGCG	1 cut(s)	335
MwoI	GCNNNNNNNGC	6 cut(s)	734, 815, 1274, 1304, 1317, 1451
NciI	CCSGG	4 cut(s)	634, 1122, 1539, 1892
NcoI	CCATGG	1 cut(s)	1730
NdeI	CATATG	1 cut(s)	1858
NdeII	GATC	9 cut(s)	137, 316, 336, 345, 1270, 1360, 1474, 1546, 1935
NheI	GCTAGC	1 cut(s)	1783
NlaIII	CATG	7 cut(s)	102, 1288, 1433, 1475, 1530, 1717, 1734
NlaIV	GGNNCC	6 cut(s)	181, 574, 624, 778, 825, 1387
NmeAIII	GCCGAG	1 cut(s)	367
NmuCI	GTSAC	4 cut(s)	3, 101, 1141, 1675
NruI	TCGCGA	1 cut(s)	335
NsbI	TGCGCA	1 cut(s)	1453
NspI	RCATGY	1 cut(s)	1717
PaeR7I	CTCGAG	1 cut(s)	1515
PagI	TCATGA	1 cut(s)	1284
PaqCI	CACCTGC	1 cut(s)	16
PcsI	WCGNNNNNNNCGW	1 cut(s)	339
PfeI	GAWTC	5 cut(s)	267, 1018, 1103, 1300, 1352
PinAI	ACCGGT	1 cut(s)	1649
Ple19I	CGATCG	1 cut(s)	339
PleI	GAGTC	3 cut(s)	159, 386, 1456
PpsI	GAGTC	3 cut(s)	159, 386, 1456
Psp6I	CCWGG	6 cut(s)	51, 175, 394, 772, 1252, 1465
PspFI	CCCAGC	3 cut(s)	656, 793, 1747
PspGI	CCWGG	6 cut(s)	51, 175, 394, 772, 1252, 1465
PspN4I	GGNNCC	6 cut(s)	181, 574, 624, 778, 825, 1387
PspPI	GGNCC	7 cut(s)	179, 572, 767, 777, 1119, 1727, 1753
PspXI	VCTCGAGB	1 cut(s)	1515
PstI	CTGCAG	1 cut(s)	484
PsuI	RGATCY	5 cut(s)	137, 345, 1270, 1546, 1935
PvuI	CGATCG	1 cut(s)	339
PvuII	CAGCTG	2 cut(s)	1024, 1573
RgaI	GCGATCGC	1 cut(s)	339
RruI	TCGCGA	1 cut(s)	335
RsaI	GTAC	2 cut(s)	278, 486
RsaNI	GTAC	2 cut(s)	277, 485
RseI	CAYNNNNRTG	2 cut(s)	860, 1391
SaqAI	TTAA	3 cut(s)	146, 1164, 1184
Sau3AI	GATC	9 cut(s)	137, 316, 336, 345, 1270, 1360, 1474, 1546, 1935
Sau96I	GGNCC	7 cut(s)	179, 572, 767, 777, 1119, 1727, 1753
ScaI	AGTACT	2 cut(s)	278, 486
SchI	GAGTC	3 cut(s)	159, 387, 1456
SduI	GDGCHC	1 cut(s)	230
SfaAI	GCGATCGC	1 cut(s)	339
SfaNI	GCATC	3 cut(s)	245, 943, 1770
SfcI	CTRYAG	1 cut(s)	480
Sfr274I	CTCGAG	1 cut(s)	1515
SgfI	GCGATCGC	1 cut(s)	339
SinI	GGWCC	3 cut(s)	179, 777, 1727
SlaI	CTCGAG	1 cut(s)	1515
SmiMI	CAYNNNNRTG	2 cut(s)	860, 1391
SmlI	CTYRAG	2 cut(s)	262, 1515
SmoI	CTYRAG	2 cut(s)	262, 1515
SsiI	CCGC	4 cut(s)	821, 1263, 1551, 1751
SspMI	CTAG	6 cut(s)	425, 885, 1581, 1598, 1784, 2038
StyI	CCWWGG	3 cut(s)	63, 458, 1730
TaaI	ACNGT	4 cut(s)	331, 1058, 1147, 1705
TaqI	TCGA	5 cut(s)	133, 663, 1355, 1363, 1516
TaqII	GACCGA	1 cut(s)	2005
TatI	WGTACW	2 cut(s)	276, 484
TauI	GCSGC	1 cut(s)	1754
TfiI	GAWTC	5 cut(s)	267, 1018, 1103, 1300, 1352
Tru1I	TTAA	3 cut(s)	146, 1164, 1184
Tru9I	TTAA	3 cut(s)	146, 1164, 1184
TscAI	CASTG	5 cut(s)	1063, 1485, 1710, 1768, 1839
TseFI	GTSAC	4 cut(s)	3, 101, 1141, 1675
TseI	GCWGC	9 cut(s)	449, 725, 818, 1024, 1076, 1295, 1610, 1655, 1669
Tsp45I	GTSAC	4 cut(s)	3, 101, 1141, 1675
TspRI	CASTG	5 cut(s)	1063, 1485, 1710, 1768, 1839
VpaK11BI	GGWCC	3 cut(s)	179, 777, 1727
XapI	RAATTY	9 cut(s)	57, 148, 502, 611, 698, 741, 919, 1095, 1976
XbaI	TCTAGA	1 cut(s)	884
XceI	RCATGY	1 cut(s)	1717
XcmI	CCANNNNNNNNNTGG	1 cut(s)	616
XhoI	CTCGAG	1 cut(s)	1515
XmiI	GTMKAC	1 cut(s)	306
XspI	CTAG	6 cut(s)	425, 885, 1581, 1598, 1784, 2038
ZrmI	AGTACT	2 cut(s)	278, 486

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.