Rh4AG229400

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0016567
Physical Location & Seq
Forward (+)
53127340 .. 53129168
1829 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG229400.1

Sequence Viewer

Length: 1746 bp
ATGTCACTATATAAATCCCTCACTCCATACCTTTTACCCTCACTAACTACCCACCACCAAACAAAAGCACACAAGCTAAGAGCTCGATCGTTGTCCATTTTCATTTCTTCAATGGAGTTCGTTAGCAAAGACACCACCTGGTGGGTCTTTACCCTCCCAGCTTTTCTCGGCTTCTCGAACATTCTCCAACCCTACTTCCTCTTCTGTTTCGCCGTTGCTTTTGTATCCATTGCCCTTCTCACTTGGGCCTTCGCTACCGGCGGTGTCGCATGGAAAAACGGCCGGAACCAAAGAGGTCCGGTCGCCATACCCGGACCCAAAGGTCTTCCCGTGTTTGGTTCCCTCTTCAGTCTCTCCCGCGGCTTGGCTCACCGCACCTTAGCCTCCATGGCTTGGAGCCAAACCACCAGTACTCAACTCATGGCCTTCAGCCTTGGTTCCACTCCTGCTGTTGTAGCATCTGACCCTCACACAGCCCGGGAAATCCTGACCTCCCCTTACTTCGCTGACCGCCCAATAAAGCAGTCGGCTAAGAGCCTTATGTTCACCCGAGCCATGGGGTTCGCTCCCAACGGACCATACTGGAGACTCCTCAGGAAAATCTCCTCTTCCCACCTCTTTGCTCCCAGGCGCATTATAGCACACGAGGCCGGCCGCCAGCAAGATTGTGCCACCATGCTGTGCAACATTGCCAAAGAACAAGCCCGCTGCGGAACGGTGTCGCTTCGAAAGCACCTACGGACAGCGTCCCTCAACAACATCATGGGGAGCGTGTTCGGGAAAAGGTACGACCCGGCGGGGAATCATAGTGAGGATGAGCTGAGGGAGCTCGAGGATATGGTCAGGGAAGGGTTTGAGATCTTGGGTGCTTTCAACTTTTCTGACTATCTCCCTTGGTTGAGTTATTTTTACGACCCGTTCTGCATCAATGAGCGTTGCGCCAAGCTCGTCCCCCGAGTGAGGAAACTCGTCCGGAGCATCATCGAAGAGCACCGATTCGGCGCTGAGTTGAAGAAGAAGCAGCAGCTCTTGGAGGATCACGCTGATTTCGTTGATGTTTTGCTCTCTTTGGATGCGGACGAGAAGCTTGAGGAAAATGACATGATTGCTGTGCTATGGGAGATGATATTCAGAGGGACTGATACGACCGCTTTGCTGACCGAGTGGGCGATGGCCGAGTTGATCTTAAACCCCGAGACTCAAGATAAACTTTACAAAGAGATTCACAGCCTTGTGGGAGAGAGGAGCGTGACGGACGCTGACGTGGCGAGACTGCCTTACTTGCAGGCTGTGGTGAAGGAAACCCTGAGAGTGCACCCACCGGGCCCACTTCTGTCCTGGGCGAGGCTGTCAACGTCCGACGTCCAGCTCAGCAACGGGATGGTGATCCCCGCCGGGACGACGGCGATGGTCAACATGTGGGCCATAGGGCAAAACCCTGAGGTGTGGTGGGAGGCGCACGTGTTCAAGCCCGAGAGGTTTGTGGTGAGTGAAGGCGGCGCTGACGTGGACGTGAGGGGAAGGGACCTGAGGCTGGCGCCGTTCGGGGCGGGGAGGAGGGTGTGTCCGGGCAAAAATCTGGGACTGGTGACGGCGTCGCTTTGGGTGGCGAAACTGGTTCAGGGGTTCAAGTGGGCCCCGGACGAGGGGCACCCGGTTGATCTGAGTGAGTTGCTGAAGCTGTCTTGTGAGATGAAGCATGAGCTGCATGGTGTGGCTTTGCCGAGGACTGCTGCTATGGTCTAG

Protein Analysis

581

Amino Acids

64.97

Weight (kDa)

8.76

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 104 - 554 2e-82 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016567)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09970
fragaria_vesca FvH4_4g17210
malus_domestica MD00G1015100.v1.1
prunus_persica Prupe.8G046400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0421111
rosa_laevigata RLG00000007712
rosa_multiflora Rmu_co8479425.1_g000001
rosa_roxburghii Rroxscaffold_5G00363430
rosa_rugosa Rorug04G0168600
rosa_samantha Rh4AG229400 Rh4BG232200 Rh4CG243800 Rh4DG227700
rosa_wichuraiana Rw4G020430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 321
AatII GACGTC 1 cut(s) 1365
AccB1I GGYRCC 2 cut(s) 1537, 1650
AccB7I CCANNNNNTGG 2 cut(s) 141, 393
AccII CGCG 1 cut(s) 360
AccIII TCCGGA 1 cut(s) 972
AclWI GGATC 2 cut(s) 1044, 1381
AcoI YGGCCR 3 cut(s) 280, 652, 1173
AcuI CTGAAG 3 cut(s) 331, 412, 1697
AcvI CACGTG 1 cut(s) 1462
AcyI GRCGYC 3 cut(s) 1362, 1538, 1595
AdeI CACNNNGTG 1 cut(s) 141
AfaI GTAC 2 cut(s) 412, 788
AflIII ACRYGT 2 cut(s) 1416, 1461
AgsI TTSAA 5 cut(s) 111, 874, 1012, 1468, 1630
AjiI CACGTC 3 cut(s) 1264, 1507, 1513
AjnI CCWGG 3 cut(s) 137, 626, 1337
Alw21I GWGCWC 4 cut(s) 85, 831, 993, 1317
Alw26I GTCTC 4 cut(s) 356, 580, 1190, 1264
Alw44I GTGCAC 1 cut(s) 1313
AlwI GGATC 2 cut(s) 1044, 1381
Ama87I CYCGRG 6 cut(s) 477, 549, 830, 954, 1193, 1472
Aor13HI TCCGGA 1 cut(s) 972
AoxI GGCC 9 cut(s) 246, 280, 423, 648, 652, 1173, 1324, 1422, 1635
ApaI GGGCCC 2 cut(s) 1328, 1639
ApaLI GTGCAC 1 cut(s) 1313
ApeKI GCWGC 5 cut(s) 708, 1021, 1024, 1705, 1733
AspLEI GCGC 6 cut(s) 633, 941, 1004, 1459, 1502, 1540
AsuC2I CCSGG 9 cut(s) 312, 478, 479, 794, 1323, 1396, 1569, 1640, 1655
AsuHPI GGTGA 6 cut(s) 362, 538, 1306, 1396, 1498, 1600
AsuII TTCGAA 1 cut(s) 727
AvaI CYCGRG 6 cut(s) 477, 549, 830, 954, 1193, 1472
AvaII GGWCC 4 cut(s) 296, 314, 575, 1525
AxyI CCTNAGG 3 cut(s) 593, 1440, 1529
BaeGI GKGCMC 4 cut(s) 1317, 1328, 1639, 1653
BanI GGYRCC 2 cut(s) 1537, 1650
BanII GRGCYC 4 cut(s) 85, 831, 1328, 1639
BauI CACGAG 1 cut(s) 644
BbrPI CACGTG 1 cut(s) 1462
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 4 cut(s) 85, 831, 993, 1317
BbvCI CCTCAGC 1 cut(s) 821
BbvI GCAGC 5 cut(s) 695, 1033, 1036, 1692, 1720
BccI CCATC 3 cut(s) 1165, 1375, 1402
BceAI ACGGC 5 cut(s) 197, 295, 1419, 1525, 1608
BcgI CGANNNNNNTGC 2 cut(s) 1135, 1169
BciT130I CCWGG 3 cut(s) 139, 628, 1339
BciVI GTATCC 1 cut(s) 235
BcnI CCSGG 9 cut(s) 312, 478, 479, 794, 1323, 1396, 1569, 1640, 1655
BcoDI GTCTC 4 cut(s) 356, 580, 1190, 1264
BfaI CTAG 1 cut(s) 1744
BfoI RGCGCY 3 cut(s) 1005, 1503, 1541
BfuI GTATCC 1 cut(s) 235
BglI GCCNNNNNGGC 1 cut(s) 389
BglII AGATCT 1 cut(s) 858
BisI GCNGC 8 cut(s) 361, 655, 709, 1022, 1025, 1498, 1706, 1734
BlpI GCTNAGC 1 cut(s) 1370
BlsI GCNGC 8 cut(s) 362, 656, 710, 1023, 1026, 1499, 1707, 1735
BmcAI AGTACT 1 cut(s) 412
Bme18I GGWCC 4 cut(s) 296, 314, 575, 1525
BmeT110I CYCGRG 6 cut(s) 477, 549, 830, 954, 1193, 1472
BmgBI CACGTC 3 cut(s) 1264, 1507, 1513
BmsI GCATC 4 cut(s) 467, 933, 987, 1063
BpiI GAAGAC 1 cut(s) 317
BpmI CTGGAG 1 cut(s) 604
Bpu10I CCTNAGC 2 cut(s) 379, 821
Bpu1102I GCTNAGC 1 cut(s) 1370
Bpu14I TTCGAA 1 cut(s) 727
BpuEI CTTGAG 2 cut(s) 1109, 1185
BpuMI CCSGG 9 cut(s) 312, 478, 479, 794, 1323, 1396, 1569, 1640, 1655
BsaAI YACGTR 1 cut(s) 1462
BsaBI GATNNNNATC 1 cut(s) 1385
BsaHI GRCGYC 3 cut(s) 1362, 1538, 1595
BsaWI WCCGGW 2 cut(s) 298, 972
BsaXI ACNNNNNCTCC 2 cut(s) 1549, 1579
Bse118I RCCGGY 2 cut(s) 257, 650
Bse1I ACTGG 4 cut(s) 408, 587, 1590, 1620
Bse21I CCTNAGG 3 cut(s) 593, 1440, 1529
Bse3DI GCAATG 2 cut(s) 228, 687
Bse8I GATNNNNATC 1 cut(s) 1385
BseAI TCCGGA 1 cut(s) 972
BseBI CCWGG 3 cut(s) 139, 628, 1339
BseGI GGATG 3 cut(s) 820, 1078, 1386
BseJI GATNNNNATC 1 cut(s) 1385
BseMI GCAATG 2 cut(s) 228, 687
BseMII CTCAG 8 cut(s) 607, 812, 996, 1298, 1384, 1431, 1520, 1655
BseNI ACTGG 4 cut(s) 408, 587, 1590, 1620
BseRI GAGGAG 4 cut(s) 581, 595, 1258, 1570
BseSI GKGCMC 4 cut(s) 1317, 1328, 1639, 1653
BseX3I CGGCCG 2 cut(s) 280, 652
BseXI GCAGC 5 cut(s) 695, 1033, 1036, 1692, 1720
BseYI CCCAGC 1 cut(s) 157
Bsh1236I CGCG 1 cut(s) 360
Bsh1285I CGRYCG 5 cut(s) 89, 283, 303, 655, 1149
BshFI GGCC 9 cut(s) 248, 282, 425, 650, 654, 1175, 1326, 1424, 1637
BshNI GGYRCC 2 cut(s) 1537, 1650
BsiEI CGRYCG 5 cut(s) 89, 283, 303, 655, 1149
BsiHKAI GWGCWC 4 cut(s) 85, 831, 993, 1317
BsiHKCI CYCGRG 6 cut(s) 477, 549, 830, 954, 1193, 1472
BslFI GGGAC 6 cut(s) 733, 935, 1150, 1411, 1538, 1596
BsmAI GTCTC 4 cut(s) 356, 580, 1190, 1264
BsmFI GGGAC 6 cut(s) 733, 935, 1150, 1411, 1538, 1596
BsnI GGCC 9 cut(s) 248, 282, 425, 650, 654, 1175, 1326, 1424, 1637
BsoBI CYCGRG 6 cut(s) 477, 549, 830, 954, 1193, 1472
Bsp119I TTCGAA 1 cut(s) 727
Bsp120I GGGCCC 2 cut(s) 1324, 1635
Bsp1286I GDGCHC 7 cut(s) 85, 831, 993, 1317, 1328, 1639, 1653
Bsp13I TCCGGA 1 cut(s) 972
Bsp143I GATC 6 cut(s) 86, 858, 1036, 1182, 1386, 1660
Bsp1720I GCTNAGC 1 cut(s) 1370
Bsp19I CCATGG 2 cut(s) 387, 555
BspANI GGCC 9 cut(s) 248, 282, 425, 650, 654, 1175, 1326, 1424, 1637
BspCNI CTCAG 8 cut(s) 606, 813, 997, 1299, 1383, 1432, 1521, 1656
BspEI TCCGGA 1 cut(s) 972
BspFNI CGCG 1 cut(s) 360
BspPI GGATC 2 cut(s) 1044, 1381
BspQI GCTCTTC 1 cut(s) 981
BspT104I TTCGAA 1 cut(s) 727
BspT107I GGYRCC 2 cut(s) 1537, 1650
BsrDI GCAATG 2 cut(s) 228, 687
BsrFI RCCGGY 2 cut(s) 257, 650
BsrI ACTGG 4 cut(s) 408, 587, 1590, 1620
BssAI RCCGGY 2 cut(s) 257, 650
BssMI GATC 6 cut(s) 86, 858, 1036, 1182, 1386, 1660
BssNI GRCGYC 3 cut(s) 1362, 1538, 1595
BssSI CACGAG 1 cut(s) 644
BssT1I CCWWGG 4 cut(s) 387, 433, 555, 893
Bst2BI CACGAG 1 cut(s) 644
Bst2UI CCWGG 3 cut(s) 139, 628, 1339
Bst4CI ACNGT 1 cut(s) 718
Bst6I CTCTTC 4 cut(s) 206, 350, 613, 981
BstACI GRCGYC 3 cut(s) 1362, 1538, 1595
BstAPI GCANNNNNTGC 1 cut(s) 1705
BstBAI YACGTR 1 cut(s) 1462
BstBI TTCGAA 1 cut(s) 727
BstC8I GCNNGC 5 cut(s) 652, 659, 706, 1287, 1536
BstDSI CCRYGG 3 cut(s) 358, 387, 555
BstENI CCTNNNNNAGG 1 cut(s) 1342
BstF5I GGATG 3 cut(s) 820, 1078, 1386
BstFNI CGCG 1 cut(s) 360
BstH2I RGCGCY 3 cut(s) 1005, 1503, 1541
BstHHI GCGC 6 cut(s) 633, 941, 1004, 1459, 1502, 1540
BstKTI GATC 6 cut(s) 89, 861, 1039, 1185, 1389, 1663
BstMAI GTCTC 4 cut(s) 356, 580, 1190, 1264
BstMBI GATC 6 cut(s) 86, 858, 1036, 1182, 1386, 1660
BstMCI CGRYCG 5 cut(s) 89, 283, 303, 655, 1149
BstMWI GCNNNNNNNGC 8 cut(s) 389, 455, 647, 730, 826, 1265, 1282, 1705
BstNI CCWGG 3 cut(s) 139, 628, 1339
BstNSI RCATGY 1 cut(s) 1420
BstSLI GKGCMC 4 cut(s) 1317, 1328, 1639, 1653
BstUI CGCG 1 cut(s) 360
BstV1I GCAGC 5 cut(s) 695, 1033, 1036, 1692, 1720
BstV2I GAAGAC 1 cut(s) 317
BstX2I RGATCY 1 cut(s) 858
BstYI RGATCY 1 cut(s) 858
BstZI CGGCCG 2 cut(s) 280, 652
Bsu36I CCTNAGG 3 cut(s) 593, 1440, 1529
BsuI GTATCC 1 cut(s) 235
BsuRI GGCC 9 cut(s) 248, 282, 425, 650, 654, 1175, 1326, 1424, 1637
BtgI CCRYGG 3 cut(s) 358, 387, 555
BtgZI GCGATG 2 cut(s) 1184, 1421
BtrI CACGTC 3 cut(s) 1264, 1507, 1513
BtsCI GGATG 3 cut(s) 820, 1078, 1386
Cac8I GCNNGC 5 cut(s) 652, 659, 706, 1287, 1536
CfoI GCGC 6 cut(s) 633, 941, 1004, 1459, 1502, 1540
Cfr10I RCCGGY 2 cut(s) 257, 650
Cfr42I CCGCGG 1 cut(s) 361
Cfr9I CCCGGG 1 cut(s) 477
CseI GACGC 3 cut(s) 735, 1265, 1584
CsiI ACCWGGT 1 cut(s) 137
Csp6I GTAC 2 cut(s) 411, 787
CviQI GTAC 2 cut(s) 411, 787
DinI GGCGCC 1 cut(s) 1539
DpnI GATC 6 cut(s) 88, 860, 1038, 1184, 1388, 1662
DpnII GATC 6 cut(s) 86, 858, 1036, 1182, 1386, 1660
DraIII CACNNNGTG 1 cut(s) 141
DrdI GACNNNNNNGTC 1 cut(s) 321
DseDI GACNNNNNNGTC 1 cut(s) 321
EaeI YGGCCR 3 cut(s) 280, 652, 1173
EagI CGGCCG 2 cut(s) 280, 652
Eam1104I CTCTTC 4 cut(s) 206, 350, 613, 981
EarI CTCTTC 4 cut(s) 206, 350, 613, 981
Ecl136II GAGCTC 2 cut(s) 83, 829
EclXI CGGCCG 2 cut(s) 280, 652
Eco130I CCWWGG 4 cut(s) 387, 433, 555, 893
Eco24I GRGCYC 4 cut(s) 85, 831, 1328, 1639
Eco47I GGWCC 4 cut(s) 296, 314, 575, 1525
Eco52I CGGCCG 2 cut(s) 280, 652
Eco53kI GAGCTC 2 cut(s) 83, 829
Eco57I CTGAAG 3 cut(s) 331, 412, 1697
Eco72I CACGTG 1 cut(s) 1462
Eco81I CCTNAGG 3 cut(s) 593, 1440, 1529
Eco88I CYCGRG 6 cut(s) 477, 549, 830, 954, 1193, 1472
EcoICRI GAGCTC 2 cut(s) 83, 829
EcoNI CCTNNNNNAGG 1 cut(s) 1342
EcoO109I RGGNCCY 2 cut(s) 1525, 1636
EcoRII CCWGG 3 cut(s) 137, 626, 1337
EcoT14I CCWWGG 4 cut(s) 387, 433, 555, 893
EcoT38I GRGCYC 4 cut(s) 85, 831, 1328, 1639
EgeI GGCGCC 1 cut(s) 1539
EheI GGCGCC 1 cut(s) 1539
ErhI CCWWGG 4 cut(s) 387, 433, 555, 893
FalI AAGNNNNNCTT 2 cut(s) 1194, 1226
FaqI GGGAC 6 cut(s) 733, 935, 1150, 1411, 1538, 1596
FauI CCCGC 5 cut(s) 365, 713, 790, 1399, 1543
Fnu4HI GCNGC 8 cut(s) 361, 655, 709, 1022, 1025, 1498, 1706, 1734
FokI GGATG 3 cut(s) 827, 1085, 1393
FriOI GRGCYC 4 cut(s) 85, 831, 1328, 1639
FseI GGCCGGCC 1 cut(s) 654
Fsp4HI GCNGC 8 cut(s) 361, 655, 709, 1022, 1025, 1498, 1706, 1734
FspBI CTAG 1 cut(s) 1744
GlaI GCGC 6 cut(s) 632, 940, 1003, 1458, 1501, 1539
GluI GCNGC 8 cut(s) 361, 655, 709, 1022, 1025, 1498, 1706, 1734
GsaI CCCAGC 1 cut(s) 161
GsuI CTGGAG 1 cut(s) 604
HaeII RGCGCY 3 cut(s) 1005, 1503, 1541
HaeIII GGCC 9 cut(s) 248, 282, 425, 650, 654, 1175, 1326, 1424, 1637
HgaI GACGC 3 cut(s) 735, 1265, 1584
HhaI GCGC 6 cut(s) 633, 941, 1004, 1459, 1502, 1540
Hin1I GRCGYC 3 cut(s) 1362, 1538, 1595
Hin6I GCGC 6 cut(s) 631, 939, 1002, 1457, 1500, 1538
HinP1I GCGC 6 cut(s) 631, 939, 1002, 1457, 1500, 1538
HincII GTYRAC 2 cut(s) 1353, 1414
HindII GTYRAC 2 cut(s) 1353, 1414
HindIII AAGCTT 1 cut(s) 1085
HinfI GANTC 5 cut(s) 588, 802, 996, 1198, 1222
HphI GGTGA 6 cut(s) 362, 538, 1306, 1396, 1498, 1600
Hpy166II GTNNAC 5 cut(s) 546, 1315, 1353, 1414, 1510
Hpy188I TCNGA 5 cut(s) 463, 883, 1133, 1360, 1665
Hpy188III TCNNGA 6 cut(s) 175, 487, 595, 778, 973, 1202
Hpy8I GTNNAC 5 cut(s) 546, 1315, 1353, 1414, 1510
Hpy99I CGWCG 3 cut(s) 1364, 1405, 1600
HpyAV CCTTC 7 cut(s) 245, 259, 436, 842, 1291, 1487, 1515
HpyCH4III ACNGT 1 cut(s) 718
HpyCH4IV ACGT 6 cut(s) 1263, 1355, 1362, 1461, 1506, 1512
HpyCH4V TGCA 5 cut(s) 684, 924, 1285, 1315, 1708
HpyF10VI GCNNNNNNNGC 8 cut(s) 389, 455, 647, 730, 826, 1265, 1282, 1705
HpySE526I ACGT 6 cut(s) 1263, 1355, 1362, 1461, 1506, 1512
Hsp92I GRCGYC 3 cut(s) 1362, 1538, 1595
HspAI GCGC 6 cut(s) 631, 939, 1002, 1457, 1500, 1538
KasI GGCGCC 1 cut(s) 1537
Kpn2I TCCGGA 1 cut(s) 972
KroI GCCGGC 1 cut(s) 650
KroNI GCCGGC 1 cut(s) 652
KspI CCGCGG 1 cut(s) 361
Kzo9I GATC 6 cut(s) 86, 858, 1036, 1182, 1386, 1660
LguI GCTCTTC 1 cut(s) 981
LmnI GCTCC 7 cut(s) 396, 571, 628, 768, 826, 975, 1245
Lsp1109I GCAGC 5 cut(s) 695, 1033, 1036, 1692, 1720
LweI GCATC 4 cut(s) 467, 933, 987, 1063
MabI ACCWGGT 1 cut(s) 137
MaeI CTAG 1 cut(s) 1744
MaeII ACGT 6 cut(s) 1263, 1355, 1362, 1461, 1506, 1512
MaeIII GTNAC 3 cut(s) 3, 1249, 1588
MalI GATC 6 cut(s) 88, 860, 1038, 1184, 1388, 1662
MboI GATC 6 cut(s) 86, 858, 1036, 1182, 1386, 1660
MboII GAAGA 8 cut(s) 99, 193, 317, 337, 600, 998, 1024, 1027
MflI RGATCY 1 cut(s) 858
MhlI GDGCHC 7 cut(s) 85, 831, 993, 1317, 1328, 1639, 1653
Mly113I GGCGCC 1 cut(s) 1538
MlyI GAGTC 2 cut(s) 582, 1192
MmeI TCCRAC 2 cut(s) 211, 1383
MroI TCCGGA 1 cut(s) 972
MroNI GCCGGC 1 cut(s) 650
MseI TTAA 1 cut(s) 1187
MspA1I CMGCKG 2 cut(s) 360, 708
MvaI CCWGG 3 cut(s) 139, 628, 1339
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 8 cut(s) 389, 455, 647, 730, 826, 1265, 1282, 1705
NaeI GCCGGC 1 cut(s) 652
NarI GGCGCC 1 cut(s) 1538
NciI CCSGG 9 cut(s) 312, 478, 479, 794, 1323, 1396, 1569, 1640, 1655
NcoI CCATGG 2 cut(s) 387, 555
NdeII GATC 6 cut(s) 86, 858, 1036, 1182, 1386, 1660
NgoMIV GCCGGC 1 cut(s) 650
NmeAIII GCCGAG 2 cut(s) 147, 1201
NmuCI GTSAC 3 cut(s) 3, 1249, 1588
NspI RCATGY 1 cut(s) 1420
NspV TTCGAA 1 cut(s) 727
PaeR7I CTCGAG 1 cut(s) 830
PciI ACATGT 1 cut(s) 1416
PciSI GCTCTTC 1 cut(s) 981
PcsI WCGNNNNNNNCGW 1 cut(s) 1047
PdiI GCCGGC 1 cut(s) 652
PfeI GAWTC 3 cut(s) 802, 996, 1222
PflFI GACNNNGTC 2 cut(s) 745, 1594
PflMI CCANNNNNTGG 2 cut(s) 141, 393
PkrI GCNGC 8 cut(s) 362, 656, 710, 1023, 1026, 1499, 1707, 1735
Ple19I CGATCG 1 cut(s) 89
PleI GAGTC 2 cut(s) 582, 1192
PluTI GGCGCC 1 cut(s) 1541
PmaCI CACGTG 1 cut(s) 1462
PmlI CACGTG 1 cut(s) 1462
PpsI GAGTC 2 cut(s) 582, 1192
Ppu21I YACGTR 1 cut(s) 1462
PpuMI RGGWCCY 1 cut(s) 1525
PscI ACATGT 1 cut(s) 1416
Psp124BI GAGCTC 2 cut(s) 85, 831
Psp5II RGGWCCY 1 cut(s) 1525
Psp6I CCWGG 3 cut(s) 137, 626, 1337
PspCI CACGTG 1 cut(s) 1462
PspFI CCCAGC 1 cut(s) 157
PspGI CCWGG 3 cut(s) 137, 626, 1337
PspOMI GGGCCC 2 cut(s) 1324, 1635
PspPPI RGGWCCY 1 cut(s) 1525
PspXI VCTCGAGB 1 cut(s) 830
PsuI RGATCY 1 cut(s) 858
PsyI GACNNNGTC 2 cut(s) 745, 1594
PvuI CGATCG 1 cut(s) 89
RigI GGCCGGCC 1 cut(s) 654
RsaI GTAC 2 cut(s) 412, 788
RsaNI GTAC 2 cut(s) 411, 787
SacI GAGCTC 2 cut(s) 85, 831
SacII CCGCGG 1 cut(s) 361
SapI GCTCTTC 1 cut(s) 981
SaqAI TTAA 1 cut(s) 1187
SatI GCNGC 8 cut(s) 361, 655, 709, 1022, 1025, 1498, 1706, 1734
Sau3AI GATC 6 cut(s) 86, 858, 1036, 1182, 1386, 1660
ScaI AGTACT 1 cut(s) 412
SchI GAGTC 2 cut(s) 582, 1192
SduI GDGCHC 7 cut(s) 85, 831, 993, 1317, 1328, 1639, 1653
SexAI ACCWGGT 1 cut(s) 137
SfaNI GCATC 4 cut(s) 467, 933, 987, 1063
SfoI GGCGCC 1 cut(s) 1539
Sfr274I CTCGAG 1 cut(s) 830
Sfr303I CCGCGG 1 cut(s) 361
SfuI TTCGAA 1 cut(s) 727
SgrBI CCGCGG 1 cut(s) 361
SinI GGWCC 4 cut(s) 296, 314, 575, 1525
SlaI CTCGAG 1 cut(s) 830
SmaI CCCGGG 1 cut(s) 479
SmlI CTYRAG 3 cut(s) 830, 1088, 1200
SmoI CTYRAG 3 cut(s) 830, 1088, 1200
SspDI GGCGCC 1 cut(s) 1537
SspMI CTAG 1 cut(s) 1744
SstI GAGCTC 2 cut(s) 85, 831
StyI CCWWGG 4 cut(s) 387, 433, 555, 893
TaaI ACNGT 1 cut(s) 718
TaiI ACGT 6 cut(s) 1266, 1358, 1365, 1464, 1509, 1515
TaqI TCGA 5 cut(s) 85, 176, 727, 831, 984
TaqII GACCGA 1 cut(s) 1175
TatI WGTACW 1 cut(s) 410
TauI GCSGC 3 cut(s) 363, 657, 1500
TfiI GAWTC 3 cut(s) 802, 996, 1222
Tru1I TTAA 1 cut(s) 1187
Tru9I TTAA 1 cut(s) 1187
TseFI GTSAC 3 cut(s) 3, 1249, 1588
TseI GCWGC 5 cut(s) 708, 1021, 1024, 1705, 1733
Tsp45I GTSAC 3 cut(s) 3, 1249, 1588
TspDTI ATGAA 2 cut(s) 91, 1709
TspGWI ACGGA 3 cut(s) 588, 754, 1268
TspMI CCCGGG 1 cut(s) 477
Tth111I GACNNNGTC 2 cut(s) 745, 1594
Van91I CCANNNNNTGG 2 cut(s) 141, 393
VneI GTGCAC 1 cut(s) 1313
VpaK11BI GGWCC 4 cut(s) 296, 314, 575, 1525
XagI CCTNNNNNAGG 1 cut(s) 1342
XceI RCATGY 1 cut(s) 1420
XcmI CCANNNNNNNNNTGG 1 cut(s) 1335
XhoI CTCGAG 1 cut(s) 830
XmaI CCCGGG 1 cut(s) 477
XspI CTAG 1 cut(s) 1744
ZraI GACGTC 1 cut(s) 1363
ZrmI AGTACT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.