Rh4DG227700

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0016567
Physical Location & Seq
Forward (+)
43268126 .. 43270225
2100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG227700.1

Sequence Viewer

Length: 1743 bp
ATGTCACTATATAAATCCCTCACCCCATACCTTTTACCCTCACTAACTACCCACCACCAAACAAAAGCACACAAGCTAAGAGCTCGATCGTTGTCCATTTTCATTTCTTCAATGGAGTTCGTTAGCAAAGACACCACCTGGTGGGTCTTTACCCTCCCAGCTTTTCTCGGCTTCTCGAACATTCTCCAACCCTACTTCCTCTTCTGTTTCGCCGTTGCTTTTGTATCCATTGCCCTTCTCACTTGGGCCTTCGCAACCGGCGGTGTGGGATGGAAAAACGGCCGGAACCAAAGAGGTCCGGTCGCCATACCCGGACCCAAAGGCCTTCCCGTGTTTGGTTCCCTGTTCAGTCTCTCCCGCGGCTTGGCTCACCGCACCTTAGCCTCCATGGCTTGGAGCCAAACCACCACTACTCAACTCATGGCCTTCAGTCTTGGCTCCACTCCTGCTGTTGTAGCATCTGACCCTCACACAGCCCGGGAAATCCTGACCTCCCCTTACTTCGCTGACCGCCCAATAAAGCAGTCGGCTAAGAGCCTTATGTTCACCCGAGCCATGGGGTTCGCTCCCAACGGACCATACTGGAGACTCCTCAGGAAAATCTCCTCTTCCCACCTCTTTGCTCCCAGGCGCATTATAGCACACGAGGCCGGCCGGCAGCAAGATTGCGCCACCATGCTGTACAATATTGCAGAAGAACAAGCCAGCTGCGGAACGGTGTCGCTTCGAAAGCACCTACGGACAGCGTCCCTCAACAACATCATGGGGAGCGTGTTCGGGAAAAGGTACGACCCGGCGGGGAATCATAGCGAGGAGGAGCTGAGGGAGCTCGAGGATATGGTCAGGGAAGGGTTTGAGATCTTGGGTGCTTTCAACTTTTCTGACTATCTCCCTTGGTTAAGTTATTTTTACGACCCGTTCTGCATCCATGAGCGTTGCGCCAAGCTCGTCCCCCGAGTGAGGAAACTCGTCCGGAGCATCATCGAAGAGCACCGACTCAGCGCTGAGTTGAAGAAGCAGCAGCTCTTGGAGGATCACGCTGATTTCGTTGATGTTTTGCTCTCTTTGGATGGGGACGAGAAGCTTGAGGAAAATGACATGATTGCTGTGCTATGGGAGATGATATTCAGAGGGACTGATACGACCGCTTTGCTGACCGAGTGGGCGATGGCCGAGTTGATCTTAAACCCCGAGACTCAAGATAAACTTTACAAAGAGATTCACAGCCTTGTGGGAGAGAGGAGCGTGACGGACGCTGACGTGGCGAGACTGCCTTACTTGCAGGCTGTGGTGAAGGAAACCCTGAGAGTGCACCCACCGGGCCCACTTCTGTCCTGGGCGAGGCTGTCAACGTCCGACGTCCAGCTCAGCAACGGGATGGTGATCCCTGCCGGGACGACGGCGATGGTCAACATGTGGGCCATTGGGCAAAACCCTGAGGTGTGGGGGGAGGCGCACGTGTTCAAGCCCGAGAGGTTTGTGGTGAGTGAAGGCGGCGCTGACGTGGACGTGAGGGGAAGGGACCTGAGGCTGGCGCCGTTCGGGGCGGGGAGGAGGGTGTGTCCGGGCAAAAATCTGGGACTGGTGACGGCGTCGCTTTGGGTGGCGAAGCTGGTTCAGGGGTTCAAGTGGGCCCCGGACGAGGGGCACCCGGTTGATCTGAGTGAGTTGCTGAAGCTGTCTTGTGAGATGAAGCATGAGCTGCATGGTGTGGCTTTGCCGAGGACTGCTGCTATGGTCTAG

Protein Analysis

580

Amino Acids

64.73

Weight (kDa)

8.09

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 104 - 553 5.2e-84 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016567)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09970
fragaria_vesca FvH4_4g17210
malus_domestica MD00G1015100.v1.1
prunus_persica Prupe.8G046400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0421111
rosa_laevigata RLG00000007712
rosa_multiflora Rmu_co8479425.1_g000001
rosa_roxburghii Rroxscaffold_5G00363430
rosa_rugosa Rorug04G0168600
rosa_samantha Rh4AG229400 Rh4BG232200 Rh4CG243800 Rh4DG227700
rosa_wichuraiana Rw4G020430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1362
AccB1I GGYRCC 2 cut(s) 1534, 1647
AccB7I CCANNNNNTGG 2 cut(s) 141, 393
AccII CGCG 1 cut(s) 360
AccIII TCCGGA 1 cut(s) 972
AclWI GGATC 2 cut(s) 1041, 1378
AcoI YGGCCR 3 cut(s) 280, 652, 1170
AcuI CTGAAG 2 cut(s) 412, 1694
AcvI CACGTG 1 cut(s) 1459
AcyI GRCGYC 3 cut(s) 1359, 1535, 1592
AdeI CACNNNGTG 1 cut(s) 141
AfaI GTAC 2 cut(s) 683, 788
AfeI AGCGCT 1 cut(s) 1003
AflIII ACRYGT 2 cut(s) 1413, 1458
AgsI TTSAA 5 cut(s) 111, 874, 1012, 1465, 1627
AjiI CACGTC 3 cut(s) 1261, 1504, 1510
AjnI CCWGG 3 cut(s) 137, 626, 1334
Alw21I GWGCWC 4 cut(s) 85, 831, 993, 1314
Alw26I GTCTC 4 cut(s) 356, 580, 1187, 1261
Alw44I GTGCAC 1 cut(s) 1310
AlwI GGATC 2 cut(s) 1041, 1378
Ama87I CYCGRG 6 cut(s) 477, 549, 830, 954, 1190, 1469
Aor13HI TCCGGA 1 cut(s) 972
Aor51HI AGCGCT 1 cut(s) 1003
ApaI GGGCCC 2 cut(s) 1325, 1636
ApaLI GTGCAC 1 cut(s) 1310
ApeKI GCWGC 6 cut(s) 658, 708, 1018, 1021, 1702, 1730
AspLEI GCGC 7 cut(s) 633, 671, 941, 1004, 1456, 1499, 1537
AsuC2I CCSGG 9 cut(s) 312, 478, 479, 794, 1320, 1393, 1566, 1637, 1652
AsuHPI GGTGA 7 cut(s) 13, 362, 538, 1303, 1393, 1495, 1597
AsuII TTCGAA 1 cut(s) 727
AvaI CYCGRG 6 cut(s) 477, 549, 830, 954, 1190, 1469
AvaII GGWCC 4 cut(s) 296, 314, 575, 1522
AxyI CCTNAGG 3 cut(s) 593, 1437, 1526
BaeGI GKGCMC 4 cut(s) 1314, 1325, 1636, 1650
BanI GGYRCC 2 cut(s) 1534, 1647
BanII GRGCYC 4 cut(s) 85, 831, 1325, 1636
BauI CACGAG 1 cut(s) 644
BbrPI CACGTG 1 cut(s) 1459
Bbv12I GWGCWC 4 cut(s) 85, 831, 993, 1314
BbvCI CCTCAGC 1 cut(s) 821
BbvI GCAGC 6 cut(s) 670, 695, 1030, 1033, 1689, 1717
BccI CCATC 5 cut(s) 264, 1064, 1162, 1372, 1399
BceAI ACGGC 5 cut(s) 197, 295, 1416, 1522, 1605
BcgI CGANNNNNNTGC 2 cut(s) 1132, 1166
BciT130I CCWGG 3 cut(s) 139, 628, 1336
BciVI GTATCC 1 cut(s) 235
BcnI CCSGG 9 cut(s) 312, 478, 479, 794, 1320, 1393, 1566, 1637, 1652
BcoDI GTCTC 4 cut(s) 356, 580, 1187, 1261
BfaI CTAG 1 cut(s) 1741
BfoI RGCGCY 3 cut(s) 1005, 1500, 1538
BfuI GTATCC 1 cut(s) 235
BglI GCCNNNNNGGC 1 cut(s) 389
BglII AGATCT 1 cut(s) 858
BisI GCNGC 8 cut(s) 361, 659, 709, 1019, 1022, 1495, 1703, 1731
BlpI GCTNAGC 1 cut(s) 1367
BlsI GCNGC 8 cut(s) 362, 660, 710, 1020, 1023, 1496, 1704, 1732
Bme18I GGWCC 4 cut(s) 296, 314, 575, 1522
BmeT110I CYCGRG 6 cut(s) 477, 549, 830, 954, 1190, 1469
BmgBI CACGTC 3 cut(s) 1261, 1504, 1510
BmsI GCATC 3 cut(s) 467, 933, 987
BpmI CTGGAG 1 cut(s) 604
Bpu10I CCTNAGC 2 cut(s) 379, 821
Bpu1102I GCTNAGC 1 cut(s) 1367
Bpu14I TTCGAA 1 cut(s) 727
BpuEI CTTGAG 2 cut(s) 1106, 1182
BpuMI CCSGG 9 cut(s) 312, 478, 479, 794, 1320, 1393, 1566, 1637, 1652
BsaAI YACGTR 1 cut(s) 1459
BsaBI GATNNNNATC 1 cut(s) 1382
BsaHI GRCGYC 3 cut(s) 1359, 1535, 1592
BsaJI CCNNGG 9 cut(s) 358, 387, 477, 555, 626, 893, 1335, 1635, 1721
BsaWI WCCGGW 2 cut(s) 298, 972
BsaXI ACNNNNNCTCC 2 cut(s) 1546, 1576
Bse118I RCCGGY 3 cut(s) 257, 650, 654
Bse1I ACTGG 2 cut(s) 587, 1587
Bse21I CCTNAGG 3 cut(s) 593, 1437, 1526
Bse3DI GCAATG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 1382
BseAI TCCGGA 1 cut(s) 972
BseBI CCWGG 3 cut(s) 139, 628, 1336
BseDI CCNNGG 9 cut(s) 358, 387, 477, 555, 626, 893, 1335, 1635, 1721
BseGI GGATG 4 cut(s) 275, 924, 1075, 1383
BseJI GATNNNNATC 1 cut(s) 1382
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 9 cut(s) 607, 812, 996, 1012, 1295, 1381, 1428, 1517, 1652
BseNI ACTGG 2 cut(s) 587, 1587
BseRI GAGGAG 6 cut(s) 581, 595, 827, 830, 1255, 1567
BseSI GKGCMC 4 cut(s) 1314, 1325, 1636, 1650
BseX3I CGGCCG 2 cut(s) 280, 652
BseXI GCAGC 6 cut(s) 670, 695, 1030, 1033, 1689, 1717
BseYI CCCAGC 1 cut(s) 157
Bsh1236I CGCG 1 cut(s) 360
Bsh1285I CGRYCG 5 cut(s) 89, 283, 303, 655, 1146
BshNI GGYRCC 2 cut(s) 1534, 1647
BsiEI CGRYCG 5 cut(s) 89, 283, 303, 655, 1146
BsiHKAI GWGCWC 4 cut(s) 85, 831, 993, 1314
BsiHKCI CYCGRG 6 cut(s) 477, 549, 830, 954, 1190, 1469
BslFI GGGAC 7 cut(s) 733, 935, 1088, 1147, 1408, 1535, 1593
BsmAI GTCTC 4 cut(s) 356, 580, 1187, 1261
BsmFI GGGAC 7 cut(s) 733, 935, 1088, 1147, 1408, 1535, 1593
BsoBI CYCGRG 6 cut(s) 477, 549, 830, 954, 1190, 1469
Bsp119I TTCGAA 1 cut(s) 727
Bsp120I GGGCCC 2 cut(s) 1321, 1632
Bsp1286I GDGCHC 7 cut(s) 85, 831, 993, 1314, 1325, 1636, 1650
Bsp13I TCCGGA 1 cut(s) 972
Bsp1407I TGTACA 1 cut(s) 681
Bsp143I GATC 6 cut(s) 86, 858, 1033, 1179, 1383, 1657
Bsp1720I GCTNAGC 1 cut(s) 1367
Bsp19I CCATGG 2 cut(s) 387, 555
BspCNI CTCAG 9 cut(s) 606, 813, 997, 1011, 1296, 1380, 1429, 1518, 1653
BspEI TCCGGA 1 cut(s) 972
BspFNI CGCG 1 cut(s) 360
BspPI GGATC 2 cut(s) 1041, 1378
BspQI GCTCTTC 1 cut(s) 981
BspT104I TTCGAA 1 cut(s) 727
BspT107I GGYRCC 2 cut(s) 1534, 1647
BsrDI GCAATG 1 cut(s) 228
BsrFI RCCGGY 3 cut(s) 257, 650, 654
BsrGI TGTACA 1 cut(s) 681
BsrI ACTGG 2 cut(s) 587, 1587
BssAI RCCGGY 3 cut(s) 257, 650, 654
BssECI CCNNGG 9 cut(s) 358, 387, 477, 555, 626, 893, 1335, 1635, 1721
BssMI GATC 6 cut(s) 86, 858, 1033, 1179, 1383, 1657
BssNI GRCGYC 3 cut(s) 1359, 1535, 1592
BssSI CACGAG 1 cut(s) 644
BssT1I CCWWGG 3 cut(s) 387, 555, 893
Bst2BI CACGAG 1 cut(s) 644
Bst2UI CCWGG 3 cut(s) 139, 628, 1336
Bst4CI ACNGT 1 cut(s) 718
Bst6I CTCTTC 3 cut(s) 206, 613, 981
BstACI GRCGYC 3 cut(s) 1359, 1535, 1592
BstAPI GCANNNNNTGC 1 cut(s) 1702
BstAUI TGTACA 1 cut(s) 681
BstBAI YACGTR 1 cut(s) 1459
BstBI TTCGAA 1 cut(s) 727
BstC8I GCNNGC 5 cut(s) 652, 656, 706, 1284, 1533
BstDSI CCRYGG 3 cut(s) 358, 387, 555
BstENI CCTNNNNNAGG 1 cut(s) 1339
BstF5I GGATG 4 cut(s) 275, 924, 1075, 1383
BstFNI CGCG 1 cut(s) 360
BstH2I RGCGCY 3 cut(s) 1005, 1500, 1538
BstHHI GCGC 7 cut(s) 633, 671, 941, 1004, 1456, 1499, 1537
BstKTI GATC 6 cut(s) 89, 861, 1036, 1182, 1386, 1660
BstMAI GTCTC 4 cut(s) 356, 580, 1187, 1261
BstMBI GATC 6 cut(s) 86, 858, 1033, 1179, 1383, 1657
BstMCI CGRYCG 5 cut(s) 89, 283, 303, 655, 1146
BstMWI GCNNNNNNNGC 8 cut(s) 389, 455, 647, 730, 826, 1262, 1279, 1702
BstNI CCWGG 3 cut(s) 139, 628, 1336
BstNSI RCATGY 1 cut(s) 1417
BstSLI GKGCMC 4 cut(s) 1314, 1325, 1636, 1650
BstUI CGCG 1 cut(s) 360
BstV1I GCAGC 6 cut(s) 670, 695, 1030, 1033, 1689, 1717
BstX2I RGATCY 1 cut(s) 858
BstYI RGATCY 1 cut(s) 858
BstZI CGGCCG 2 cut(s) 280, 652
Bsu36I CCTNAGG 3 cut(s) 593, 1437, 1526
BsuI GTATCC 1 cut(s) 235
BtgI CCRYGG 3 cut(s) 358, 387, 555
BtgZI GCGATG 2 cut(s) 1181, 1418
BtrI CACGTC 3 cut(s) 1261, 1504, 1510
BtsCI GGATG 4 cut(s) 275, 924, 1075, 1383
Cac8I GCNNGC 5 cut(s) 652, 656, 706, 1284, 1533
CfoI GCGC 7 cut(s) 633, 671, 941, 1004, 1456, 1499, 1537
Cfr10I RCCGGY 3 cut(s) 257, 650, 654
Cfr42I CCGCGG 1 cut(s) 361
Cfr9I CCCGGG 1 cut(s) 477
CseI GACGC 3 cut(s) 735, 1262, 1581
CsiI ACCWGGT 1 cut(s) 137
Csp6I GTAC 2 cut(s) 682, 787
CviQI GTAC 2 cut(s) 682, 787
DinI GGCGCC 1 cut(s) 1536
DpnI GATC 6 cut(s) 88, 860, 1035, 1181, 1385, 1659
DpnII GATC 6 cut(s) 86, 858, 1033, 1179, 1383, 1657
DraIII CACNNNGTG 1 cut(s) 141
EaeI YGGCCR 3 cut(s) 280, 652, 1170
EagI CGGCCG 2 cut(s) 280, 652
Eam1104I CTCTTC 3 cut(s) 206, 613, 981
EarI CTCTTC 3 cut(s) 206, 613, 981
Ecl136II GAGCTC 2 cut(s) 83, 829
EclXI CGGCCG 2 cut(s) 280, 652
Eco130I CCWWGG 3 cut(s) 387, 555, 893
Eco147I AGGCCT 1 cut(s) 324
Eco24I GRGCYC 4 cut(s) 85, 831, 1325, 1636
Eco47I GGWCC 4 cut(s) 296, 314, 575, 1522
Eco47III AGCGCT 1 cut(s) 1003
Eco52I CGGCCG 2 cut(s) 280, 652
Eco53kI GAGCTC 2 cut(s) 83, 829
Eco57I CTGAAG 2 cut(s) 412, 1694
Eco72I CACGTG 1 cut(s) 1459
Eco81I CCTNAGG 3 cut(s) 593, 1437, 1526
Eco88I CYCGRG 6 cut(s) 477, 549, 830, 954, 1190, 1469
EcoICRI GAGCTC 2 cut(s) 83, 829
EcoNI CCTNNNNNAGG 1 cut(s) 1339
EcoO109I RGGNCCY 2 cut(s) 1522, 1633
EcoRII CCWGG 3 cut(s) 137, 626, 1334
EcoT14I CCWWGG 3 cut(s) 387, 555, 893
EcoT38I GRGCYC 4 cut(s) 85, 831, 1325, 1636
EgeI GGCGCC 1 cut(s) 1536
EheI GGCGCC 1 cut(s) 1536
ErhI CCWWGG 3 cut(s) 387, 555, 893
FalI AAGNNNNNCTT 2 cut(s) 1191, 1223
FaqI GGGAC 7 cut(s) 733, 935, 1088, 1147, 1408, 1535, 1593
FauI CCCGC 3 cut(s) 365, 790, 1540
Fnu4HI GCNGC 8 cut(s) 361, 659, 709, 1019, 1022, 1495, 1703, 1731
FokI GGATG 4 cut(s) 282, 911, 1082, 1390
FriOI GRGCYC 4 cut(s) 85, 831, 1325, 1636
FseI GGCCGGCC 1 cut(s) 654
Fsp4HI GCNGC 8 cut(s) 361, 659, 709, 1019, 1022, 1495, 1703, 1731
FspBI CTAG 1 cut(s) 1741
GlaI GCGC 7 cut(s) 632, 670, 940, 1003, 1455, 1498, 1536
GluI GCNGC 8 cut(s) 361, 659, 709, 1019, 1022, 1495, 1703, 1731
GsaI CCCAGC 1 cut(s) 161
GsuI CTGGAG 1 cut(s) 604
HaeII RGCGCY 3 cut(s) 1005, 1500, 1538
HgaI GACGC 3 cut(s) 735, 1262, 1581
HhaI GCGC 7 cut(s) 633, 671, 941, 1004, 1456, 1499, 1537
Hin1I GRCGYC 3 cut(s) 1359, 1535, 1592
Hin6I GCGC 7 cut(s) 631, 669, 939, 1002, 1454, 1497, 1535
HinP1I GCGC 7 cut(s) 631, 669, 939, 1002, 1454, 1497, 1535
HincII GTYRAC 2 cut(s) 1350, 1411
HindII GTYRAC 2 cut(s) 1350, 1411
HindIII AAGCTT 1 cut(s) 1082
HinfI GANTC 5 cut(s) 588, 802, 996, 1195, 1219
HphI GGTGA 7 cut(s) 13, 362, 538, 1303, 1393, 1495, 1597
Hpy166II GTNNAC 5 cut(s) 546, 1312, 1350, 1411, 1507
Hpy188I TCNGA 5 cut(s) 463, 883, 1130, 1357, 1662
Hpy188III TCNNGA 6 cut(s) 175, 487, 595, 778, 973, 1199
Hpy8I GTNNAC 5 cut(s) 546, 1312, 1350, 1411, 1507
Hpy99I CGWCG 3 cut(s) 1361, 1402, 1597
HpyAV CCTTC 8 cut(s) 245, 259, 335, 436, 842, 1288, 1484, 1512
HpyCH4III ACNGT 1 cut(s) 718
HpyCH4IV ACGT 6 cut(s) 1260, 1352, 1359, 1458, 1503, 1509
HpyCH4V TGCA 5 cut(s) 692, 924, 1282, 1312, 1705
HpyF10VI GCNNNNNNNGC 8 cut(s) 389, 455, 647, 730, 826, 1262, 1279, 1702
HpySE526I ACGT 6 cut(s) 1260, 1352, 1359, 1458, 1503, 1509
Hsp92I GRCGYC 3 cut(s) 1359, 1535, 1592
HspAI GCGC 7 cut(s) 631, 669, 939, 1002, 1454, 1497, 1535
KasI GGCGCC 1 cut(s) 1534
Kpn2I TCCGGA 1 cut(s) 972
KroI GCCGGC 2 cut(s) 650, 654
KroNI GCCGGC 2 cut(s) 652, 656
KspI CCGCGG 1 cut(s) 361
Kzo9I GATC 6 cut(s) 86, 858, 1033, 1179, 1383, 1657
LguI GCTCTTC 1 cut(s) 981
LmnI GCTCC 9 cut(s) 396, 443, 571, 628, 768, 817, 826, 975, 1242
Lsp1109I GCAGC 6 cut(s) 670, 695, 1030, 1033, 1689, 1717
LweI GCATC 3 cut(s) 467, 933, 987
MabI ACCWGGT 1 cut(s) 137
MaeI CTAG 1 cut(s) 1741
MaeII ACGT 6 cut(s) 1260, 1352, 1359, 1458, 1503, 1509
MaeIII GTNAC 3 cut(s) 3, 1246, 1585
MalI GATC 6 cut(s) 88, 860, 1035, 1181, 1385, 1659
MboI GATC 6 cut(s) 86, 858, 1033, 1179, 1383, 1657
MboII GAAGA 6 cut(s) 99, 193, 600, 707, 998, 1024
MflI RGATCY 1 cut(s) 858
MhlI GDGCHC 7 cut(s) 85, 831, 993, 1314, 1325, 1636, 1650
Mly113I GGCGCC 1 cut(s) 1535
MlyI GAGTC 3 cut(s) 582, 990, 1189
MmeI TCCRAC 2 cut(s) 211, 1380
MroI TCCGGA 1 cut(s) 972
MroNI GCCGGC 2 cut(s) 650, 654
MseI TTAA 2 cut(s) 899, 1184
MspA1I CMGCKG 2 cut(s) 360, 708
MvaI CCWGG 3 cut(s) 139, 628, 1336
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 8 cut(s) 389, 455, 647, 730, 826, 1262, 1279, 1702
NaeI GCCGGC 2 cut(s) 652, 656
NarI GGCGCC 1 cut(s) 1535
NciI CCSGG 9 cut(s) 312, 478, 479, 794, 1320, 1393, 1566, 1637, 1652
NcoI CCATGG 2 cut(s) 387, 555
NdeII GATC 6 cut(s) 86, 858, 1033, 1179, 1383, 1657
NgoMIV GCCGGC 2 cut(s) 650, 654
NmeAIII GCCGAG 2 cut(s) 147, 1198
NmuCI GTSAC 3 cut(s) 3, 1246, 1585
NspI RCATGY 1 cut(s) 1417
NspV TTCGAA 1 cut(s) 727
PaeR7I CTCGAG 1 cut(s) 830
PceI AGGCCT 1 cut(s) 324
PciI ACATGT 1 cut(s) 1413
PciSI GCTCTTC 1 cut(s) 981
PcsI WCGNNNNNNNCGW 1 cut(s) 1044
PdiI GCCGGC 2 cut(s) 652, 656
PfeI GAWTC 2 cut(s) 802, 1219
PflFI GACNNNGTC 2 cut(s) 745, 1591
PflMI CCANNNNNTGG 2 cut(s) 141, 393
PkrI GCNGC 8 cut(s) 362, 660, 710, 1020, 1023, 1496, 1704, 1732
Ple19I CGATCG 1 cut(s) 89
PleI GAGTC 3 cut(s) 582, 990, 1189
PluTI GGCGCC 1 cut(s) 1538
PmaCI CACGTG 1 cut(s) 1459
PmlI CACGTG 1 cut(s) 1459
PpsI GAGTC 3 cut(s) 582, 990, 1189
Ppu21I YACGTR 1 cut(s) 1459
PpuMI RGGWCCY 1 cut(s) 1522
PscI ACATGT 1 cut(s) 1413
Psp124BI GAGCTC 2 cut(s) 85, 831
Psp5II RGGWCCY 1 cut(s) 1522
Psp6I CCWGG 3 cut(s) 137, 626, 1334
PspCI CACGTG 1 cut(s) 1459
PspFI CCCAGC 1 cut(s) 157
PspGI CCWGG 3 cut(s) 137, 626, 1334
PspOMI GGGCCC 2 cut(s) 1321, 1632
PspPPI RGGWCCY 1 cut(s) 1522
PspXI VCTCGAGB 1 cut(s) 830
PsuI RGATCY 1 cut(s) 858
PsyI GACNNNGTC 2 cut(s) 745, 1591
PvuI CGATCG 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 708
RigI GGCCGGCC 1 cut(s) 654
RsaI GTAC 2 cut(s) 683, 788
RsaNI GTAC 2 cut(s) 682, 787
SacI GAGCTC 2 cut(s) 85, 831
SacII CCGCGG 1 cut(s) 361
SapI GCTCTTC 1 cut(s) 981
SaqAI TTAA 2 cut(s) 899, 1184
SatI GCNGC 8 cut(s) 361, 659, 709, 1019, 1022, 1495, 1703, 1731
Sau3AI GATC 6 cut(s) 86, 858, 1033, 1179, 1383, 1657
SchI GAGTC 3 cut(s) 582, 990, 1189
SduI GDGCHC 7 cut(s) 85, 831, 993, 1314, 1325, 1636, 1650
SexAI ACCWGGT 1 cut(s) 137
SfaNI GCATC 3 cut(s) 467, 933, 987
SfoI GGCGCC 1 cut(s) 1536
Sfr274I CTCGAG 1 cut(s) 830
Sfr303I CCGCGG 1 cut(s) 361
SfuI TTCGAA 1 cut(s) 727
SgrBI CCGCGG 1 cut(s) 361
SinI GGWCC 4 cut(s) 296, 314, 575, 1522
SlaI CTCGAG 1 cut(s) 830
SmaI CCCGGG 1 cut(s) 479
SmlI CTYRAG 3 cut(s) 830, 1085, 1197
SmoI CTYRAG 3 cut(s) 830, 1085, 1197
SseBI AGGCCT 1 cut(s) 324
SspDI GGCGCC 1 cut(s) 1534
SspI AATATT 1 cut(s) 688
SspMI CTAG 1 cut(s) 1741
SstI GAGCTC 2 cut(s) 85, 831
StuI AGGCCT 1 cut(s) 324
StyI CCWWGG 3 cut(s) 387, 555, 893
TaaI ACNGT 1 cut(s) 718
TaiI ACGT 6 cut(s) 1263, 1355, 1362, 1461, 1506, 1512
TaqI TCGA 5 cut(s) 85, 176, 727, 831, 984
TaqII GACCGA 1 cut(s) 1172
TatI WGTACW 1 cut(s) 681
TauI GCSGC 2 cut(s) 363, 1497
TfiI GAWTC 2 cut(s) 802, 1219
Tru1I TTAA 2 cut(s) 899, 1184
Tru9I TTAA 2 cut(s) 899, 1184
TseFI GTSAC 3 cut(s) 3, 1246, 1585
TseI GCWGC 6 cut(s) 658, 708, 1018, 1021, 1702, 1730
Tsp45I GTSAC 3 cut(s) 3, 1246, 1585
TspDTI ATGAA 2 cut(s) 91, 1706
TspGWI ACGGA 3 cut(s) 588, 754, 1265
TspMI CCCGGG 1 cut(s) 477
Tth111I GACNNNGTC 2 cut(s) 745, 1591
Van91I CCANNNNNTGG 2 cut(s) 141, 393
VneI GTGCAC 1 cut(s) 1310
VpaK11BI GGWCC 4 cut(s) 296, 314, 575, 1522
XagI CCTNNNNNAGG 1 cut(s) 1339
XceI RCATGY 1 cut(s) 1417
XcmI CCANNNNNNNNNTGG 1 cut(s) 1332
XhoI CTCGAG 1 cut(s) 830
XmaI CCCGGG 1 cut(s) 477
XspI CTAG 1 cut(s) 1741
ZraI GACGTC 1 cut(s) 1360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.