Rh4AG337300

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0021287
Physical Location & Seq
Reverse (-)
64651045 .. 64652661
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG337300.1

Sequence Viewer

Length: 600 bp
ATGGGTTATGCCCGCCAGGTGTTCGATCAAATTCCTGAACCAAATTTTGCTCTATGGAATGCATTGTTGAGAGGGTATGCAAAGAATGAGATTCATAGGGAGGTTGTAGCTTTGTTTGGTAGGATGAAGAGCATGGACATAATGCCGAATTGCTATACATTCCCCGTTGTGATCAAATGTTGTGGGAGATTGAGTAGGTTGGTAGAAGGTGAAGAGGTGCATTGTGTTGTGATAAAATGTGGGTTTAGAGCAAACCCCTTTGTGGGAACGACGCTGATCGAAATGTATGCGGCTGGGGGAGTAATTGGAGCTGCTTATAAGGTGTTTGGTGAGATGTTTGAGAGAAATGTGGTTGCTTGGACTTCCATGATTAATGGCTACATTTTGGGTGGTGATATGGTTTCTGCACAGCGCCTTTTTGATTTGGCGCCAGAACGAGATGTTGTGTTGTGGAACACTATGGTTTCTGGTTATATTGAGCAGCGGGATATGAGGCATCCTGAGACCGAGGAAATATATGAAGCCTTGAAGGGATTGACCAGTGCGCTAAGATCATCTGGATATGTACCAGACATTCTGGAGCTTGGGCATGGAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

199

Amino Acids

22.34

Weight (kDa)

5.44

Isoelectric Point (pI)

41.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 14 - 62 1.8e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021287)

Species Orthologous Gene IDs
pyrus_communis pycom15g35110
rosa_multiflora Rmu_co8271389.1_g000001
rosa_samantha Rh4AG337300 Rh4CG360000 Rh4DG339800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 318
AccB1I GGYRCC 1 cut(s) 427
AciI CCGC 3 cut(s) 13, 290, 484
AcsI RAATTY 2 cut(s) 30, 43
AcyI GRCGYC 1 cut(s) 428
AfaI GTAC 1 cut(s) 567
AfiI CCNNNNNNNGG 2 cut(s) 262, 263
AgsI TTSAA 1 cut(s) 529
AjnI CCWGG 1 cut(s) 15
AluBI AGCT 3 cut(s) 110, 311, 583
AluI AGCT 3 cut(s) 110, 311, 583
Alw26I GTCTC 2 cut(s) 497, 588
ApeKI GCWGC 2 cut(s) 311, 481
ApoI RAATTY 2 cut(s) 30, 43
AseI ATTAAT 1 cut(s) 372
AspLEI GCGC 3 cut(s) 414, 430, 547
AsuHPI GGTGA 3 cut(s) 221, 341, 404
BanI GGYRCC 1 cut(s) 427
BbvI GCAGC 2 cut(s) 298, 493
BcgI CGANNNNNNTGC 2 cut(s) 269, 303
BciT130I CCWGG 1 cut(s) 17
BclI TGATCA 1 cut(s) 171
BcoDI GTCTC 2 cut(s) 497, 588
BfaI CTAG 1 cut(s) 598
BfoI RGCGCY 2 cut(s) 415, 431
BisI GCNGC 3 cut(s) 291, 312, 482
BlsI GCNGC 3 cut(s) 292, 313, 483
Bme1390I CCNGG 1 cut(s) 17
BmiI GGNNCC 1 cut(s) 429
BmrFI CCNGG 1 cut(s) 17
BmsI GCATC 1 cut(s) 505
BpmI CTGGAG 1 cut(s) 599
BsaHI GRCGYC 1 cut(s) 428
BsaI GGTCTC 1 cut(s) 497
BsaJI CCNNGG 1 cut(s) 507
BsaXI ACNNNNNCTCC 2 cut(s) 178, 208
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 263
Bse1I ACTGG 1 cut(s) 540
BseBI CCWGG 1 cut(s) 17
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 2 cut(s) 129, 496
BseLI CCNNNNNNNGG 2 cut(s) 262, 263
BseMII CTCAG 1 cut(s) 492
BseNI ACTGG 1 cut(s) 540
BseXI GCAGC 2 cut(s) 298, 493
BseYI CCCAGC 1 cut(s) 293
BsgI GTGCAG 1 cut(s) 390
BshNI GGYRCC 1 cut(s) 427
BslI CCNNNNNNNGG 2 cut(s) 262, 263
BsmAI GTCTC 2 cut(s) 497, 588
BsmI GAATGC 1 cut(s) 64
Bso31I GGTCTC 1 cut(s) 497
Bsp143I GATC 4 cut(s) 25, 171, 276, 551
BspACI CCGC 3 cut(s) 13, 290, 484
BspCNI CTCAG 1 cut(s) 493
BspLI GGNNCC 1 cut(s) 429
BspQI GCTCTTC 1 cut(s) 122
BspT107I GGYRCC 1 cut(s) 427
BspTNI GGTCTC 1 cut(s) 497
BsrI ACTGG 1 cut(s) 540
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 4 cut(s) 25, 171, 276, 551
BssNI GRCGYC 1 cut(s) 428
Bst2UI CCWGG 1 cut(s) 17
Bst6I CTCTTC 2 cut(s) 122, 207
BstACI GRCGYC 1 cut(s) 428
BstC8I GCNNGC 1 cut(s) 13
BstDEI CTNAG 2 cut(s) 501, 548
BstF5I GGATG 2 cut(s) 129, 496
BstH2I RGCGCY 2 cut(s) 415, 431
BstHHI GCGC 3 cut(s) 414, 430, 547
BstKTI GATC 4 cut(s) 28, 174, 279, 554
BstMAI GTCTC 2 cut(s) 497, 588
BstMBI GATC 4 cut(s) 25, 171, 276, 551
BstNI CCWGG 1 cut(s) 17
BstSCI CCNGG 1 cut(s) 15
BstV1I GCAGC 2 cut(s) 298, 493
BtsCI GGATG 2 cut(s) 129, 496
BtsIMutI CAGTG 1 cut(s) 547
Cac8I GCNNGC 1 cut(s) 13
CfoI GCGC 3 cut(s) 414, 430, 547
CseI GACGC 1 cut(s) 280
Csp6I GTAC 1 cut(s) 566
CspCI CAANNNNNGTGG 2 cut(s) 163, 198
CviAII CATG 3 cut(s) 133, 367, 590
CviJI RGCY 6 cut(s) 110, 293, 311, 378, 524, 583
CviKI_1 RGCY 6 cut(s) 110, 293, 311, 378, 524, 583
CviQI GTAC 1 cut(s) 566
DdeI CTNAG 2 cut(s) 501, 548
DinI GGCGCC 1 cut(s) 429
DpnI GATC 4 cut(s) 27, 173, 278, 553
DpnII GATC 4 cut(s) 25, 171, 276, 551
Eam1104I CTCTTC 2 cut(s) 122, 207
EarI CTCTTC 2 cut(s) 122, 207
Eco31I GGTCTC 1 cut(s) 497
EcoRII CCWGG 1 cut(s) 15
EcoT22I ATGCAT 1 cut(s) 64
EgeI GGCGCC 1 cut(s) 429
EheI GGCGCC 1 cut(s) 429
FaeI CATG 3 cut(s) 136, 370, 593
FatI CATG 3 cut(s) 132, 366, 589
FauI CCCGC 2 cut(s) 20, 477
FbaI TGATCA 1 cut(s) 171
Fnu4HI GCNGC 3 cut(s) 291, 312, 482
FokI GGATG 2 cut(s) 136, 483
Fsp4HI GCNGC 3 cut(s) 291, 312, 482
FspBI CTAG 1 cut(s) 598
GlaI GCGC 3 cut(s) 413, 429, 546
GluI GCNGC 3 cut(s) 291, 312, 482
GsaI CCCAGC 1 cut(s) 297
GsuI CTGGAG 1 cut(s) 599
HaeII RGCGCY 2 cut(s) 415, 431
HgaI GACGC 1 cut(s) 280
HhaI GCGC 3 cut(s) 414, 430, 547
Hin1I GRCGYC 1 cut(s) 428
Hin1II CATG 3 cut(s) 136, 370, 593
Hin6I GCGC 3 cut(s) 412, 428, 545
HinP1I GCGC 3 cut(s) 412, 428, 545
HinfI GANTC 1 cut(s) 91
HphI GGTGA 3 cut(s) 221, 341, 404
Hpy188III TCNNGA 4 cut(s) 35, 500, 558, 578
Hpy99I CGWCG 1 cut(s) 274
HpyAV CCTTC 2 cut(s) 200, 523
HpyCH4V TGCA 4 cut(s) 62, 80, 220, 407
HpyF3I CTNAG 2 cut(s) 501, 548
Hsp92I GRCGYC 1 cut(s) 428
Hsp92II CATG 3 cut(s) 136, 370, 593
HspAI GCGC 3 cut(s) 412, 428, 545
KasI GGCGCC 1 cut(s) 427
Ksp22I TGATCA 1 cut(s) 171
Kzo9I GATC 4 cut(s) 25, 171, 276, 551
LguI GCTCTTC 1 cut(s) 122
LmnI GCTCC 2 cut(s) 308, 580
Lsp1109I GCAGC 2 cut(s) 298, 493
LweI GCATC 1 cut(s) 505
MaeI CTAG 1 cut(s) 598
MalI GATC 4 cut(s) 27, 173, 278, 553
MboI GATC 4 cut(s) 25, 171, 276, 551
MboII GAAGA 2 cut(s) 139, 224
MluCI AATT 4 cut(s) 30, 43, 148, 303
Mly113I GGCGCC 1 cut(s) 428
MnlI CCTC 5 cut(s) 65, 94, 208, 486, 502
Mph1103I ATGCAT 1 cut(s) 64
MseI TTAA 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 484
MspR9I CCNGG 1 cut(s) 17
Mva1269I GAATGC 1 cut(s) 64
MvaI CCWGG 1 cut(s) 17
NarI GGCGCC 1 cut(s) 428
NdeII GATC 4 cut(s) 25, 171, 276, 551
NlaIII CATG 3 cut(s) 136, 370, 593
NlaIV GGNNCC 1 cut(s) 429
NsiI ATGCAT 1 cut(s) 64
PciSI GCTCTTC 1 cut(s) 122
PctI GAATGC 1 cut(s) 64
PfeI GAWTC 1 cut(s) 91
PkrI GCNGC 3 cut(s) 292, 313, 483
PluTI GGCGCC 1 cut(s) 431
PshBI ATTAAT 1 cut(s) 372
PsiI TTATAA 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 15
PspFI CCCAGC 1 cut(s) 293
PspGI CCWGG 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 429
RsaI GTAC 1 cut(s) 567
RsaNI GTAC 1 cut(s) 566
SapI GCTCTTC 1 cut(s) 122
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 3 cut(s) 291, 312, 482
Sau3AI GATC 4 cut(s) 25, 171, 276, 551
ScrFI CCNGG 1 cut(s) 17
SetI ASST 9 cut(s) 21, 105, 112, 200, 211, 219, 313, 324, 585
SfaNI GCATC 1 cut(s) 505
SfoI GGCGCC 1 cut(s) 429
Sse9I AATT 4 cut(s) 30, 43, 148, 303
SsiI CCGC 3 cut(s) 13, 290, 484
SspDI GGCGCC 1 cut(s) 427
SspMI CTAG 1 cut(s) 598
StyD4I CCNGG 1 cut(s) 15
TaqI TCGA 2 cut(s) 24, 279
TaqII GACCGA 1 cut(s) 521
TasI AATT 4 cut(s) 30, 43, 148, 303
TauI GCSGC 1 cut(s) 293
TfiI GAWTC 1 cut(s) 91
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 1 cut(s) 547
TseI GCWGC 2 cut(s) 311, 481
TspDTI ATGAA 3 cut(s) 83, 140, 534
TspRI CASTG 1 cut(s) 547
VspI ATTAAT 1 cut(s) 372
XapI RAATTY 2 cut(s) 30, 43
XspI CTAG 1 cut(s) 598
Zsp2I ATGCAT 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.