Rh4CG014500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0025801
Physical Location & Seq
Forward (+)
2427416 .. 2430969
3554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG014500.1

Sequence Viewer

Length: 309 bp
ATGACCAACAGATCGGTACCATGTGAGAACGAAGGAGAGAACCGCCGCGAACAACAGAACCCGGACGAGCGTGTCGCTGAGCTGATCCATCAGGAGCTTCAGGATCGACTGATCCTCGTGCTTCTCCAGTTCGTTCCATCCGTACACCGCCCGGCGCTTCTCGACTTCCTCCTCCGACAGCCCCGAGTCGCGGCTGACCTTGAACTCGTCCTCGCATTCCTGAAGAAATTGAACCTTGTTGTTGGAAGTAAAGGATGGGGTGTAAGAACTTGCACAGCAGCTGCAGTATCCATAGTATCTTCTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

102

Amino Acids

11.44

Weight (kDa)

6.1

Isoelectric Point (pI)

45.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0025801)

Species Orthologous Gene IDs
rosa_roxburghii Rroxscaffold_3G00247960 Rroxscaffold_5G00349350
rosa_samantha Rh4CG014500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
Acc65I GGTACC 1 cut(s) 16
AccB1I GGYRCC 1 cut(s) 16
AccII CGCG 2 cut(s) 48, 191
AciI CCGC 4 cut(s) 43, 46, 148, 191
AclWI GGATC 3 cut(s) 79, 106, 111
AcuI CTGAAG 2 cut(s) 83, 242
AfaI GTAC 2 cut(s) 18, 144
AfiI CCNNNNNNNGG 1 cut(s) 190
AgsI TTSAA 2 cut(s) 203, 232
AluBI AGCT 3 cut(s) 82, 97, 281
AluI AGCT 3 cut(s) 82, 97, 281
AlwI GGATC 3 cut(s) 79, 106, 111
AlwNI CAGNNNCTG 1 cut(s) 281
Ama87I CYCGRG 1 cut(s) 183
ApeKI GCWGC 2 cut(s) 278, 281
Asp718I GGTACC 1 cut(s) 16
AspLEI GCGC 1 cut(s) 157
AsuC2I CCSGG 2 cut(s) 62, 152
AvaI CYCGRG 1 cut(s) 183
BanI GGYRCC 1 cut(s) 16
BauI CACGAG 1 cut(s) 116
BbvI GCAGC 2 cut(s) 268, 290
BccI CCATC 3 cut(s) 96, 145, 249
BciVI GTATCC 1 cut(s) 298
BcnI CCSGG 2 cut(s) 62, 152
BfmI CTRYAG 1 cut(s) 282
BfoI RGCGCY 1 cut(s) 158
BfuI GTATCC 1 cut(s) 298
BisI GCNGC 4 cut(s) 46, 192, 279, 282
BlpI GCTNAGC 1 cut(s) 78
BlsI GCNGC 4 cut(s) 47, 193, 280, 283
Bme1390I CCNGG 2 cut(s) 62, 152
BmeT110I CYCGRG 1 cut(s) 183
BmiI GGNNCC 1 cut(s) 18
BmrFI CCNGG 2 cut(s) 62, 152
BpmI CTGGAG 1 cut(s) 110
Bpu1102I GCTNAGC 1 cut(s) 78
BpuMI CCSGG 2 cut(s) 62, 152
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse1I ACTGG 1 cut(s) 127
BseGI GGATG 2 cut(s) 137, 260
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 1 cut(s) 127
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 2 cut(s) 268, 290
Bsh1236I CGCG 2 cut(s) 48, 191
BshNI GGYRCC 1 cut(s) 16
BsiHKCI CYCGRG 1 cut(s) 183
BsiSI CCGG 2 cut(s) 62, 152
BslI CCNNNNNNNGG 1 cut(s) 190
BsmI GAATGC 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 183
Bsp143I GATC 4 cut(s) 11, 84, 103, 111
Bsp1720I GCTNAGC 1 cut(s) 78
BspACI CCGC 4 cut(s) 43, 46, 148, 191
BspCNI CTCAG 1 cut(s) 70
BspFNI CGCG 2 cut(s) 48, 191
BspLI GGNNCC 1 cut(s) 18
BspMAI CTGCAG 1 cut(s) 286
BspPI GGATC 3 cut(s) 79, 106, 111
BspT107I GGYRCC 1 cut(s) 16
BsrI ACTGG 1 cut(s) 127
BssMI GATC 4 cut(s) 11, 84, 103, 111
BssSI CACGAG 1 cut(s) 116
Bst2BI CACGAG 1 cut(s) 116
BstDEI CTNAG 1 cut(s) 78
BstF5I GGATG 2 cut(s) 137, 260
BstFNI CGCG 2 cut(s) 48, 191
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 4 cut(s) 14, 87, 106, 114
BstMBI GATC 4 cut(s) 11, 84, 103, 111
BstSCI CCNGG 2 cut(s) 60, 150
BstSFI CTRYAG 1 cut(s) 282
BstUI CGCG 2 cut(s) 48, 191
BstV1I GCAGC 2 cut(s) 268, 290
BsuI GTATCC 1 cut(s) 298
BtsCI GGATG 2 cut(s) 137, 260
CaiI CAGNNNCTG 1 cut(s) 281
CfoI GCGC 1 cut(s) 157
Csp6I GTAC 2 cut(s) 17, 143
CviAII CATG 1 cut(s) 21
CviJI RGCY 5 cut(s) 82, 97, 181, 194, 281
CviKI_1 RGCY 5 cut(s) 82, 97, 181, 194, 281
CviQI GTAC 2 cut(s) 17, 143
DdeI CTNAG 1 cut(s) 78
DpnI GATC 4 cut(s) 13, 86, 105, 113
DpnII GATC 4 cut(s) 11, 84, 103, 111
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
Eco57I CTGAAG 2 cut(s) 83, 242
Eco88I CYCGRG 1 cut(s) 183
FaeI CATG 1 cut(s) 24
FaiI YATR 2 cut(s) 22, 293
FatI CATG 1 cut(s) 20
Fnu4HI GCNGC 4 cut(s) 46, 192, 279, 282
FokI GGATG 2 cut(s) 124, 267
Fsp4HI GCNGC 4 cut(s) 46, 192, 279, 282
GlaI GCGC 1 cut(s) 156
GluI GCNGC 4 cut(s) 46, 192, 279, 282
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 1 cut(s) 158
HapII CCGG 2 cut(s) 62, 152
HhaI GCGC 1 cut(s) 157
Hin1II CATG 1 cut(s) 24
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HinfI GANTC 1 cut(s) 186
HpaII CCGG 2 cut(s) 62, 152
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 2 cut(s) 176, 304
Hpy188III TCNNGA 4 cut(s) 92, 101, 161, 220
Hpy8I GTNNAC 1 cut(s) 145
HpyAV CCTTC 1 cut(s) 26
HpyCH4V TGCA 2 cut(s) 273, 284
HpyF3I CTNAG 1 cut(s) 78
Hsp92II CATG 1 cut(s) 24
HspAI GCGC 1 cut(s) 155
KpnI GGTACC 1 cut(s) 20
Kzo9I GATC 4 cut(s) 11, 84, 103, 111
LmnI GCTCC 1 cut(s) 94
LpnPI CCDG 6 cut(s) 75, 77, 86, 140, 165, 233
Lsp1109I GCAGC 2 cut(s) 268, 290
MalI GATC 4 cut(s) 13, 86, 105, 113
MboI GATC 4 cut(s) 11, 84, 103, 111
MboII GAAGA 2 cut(s) 235, 291
MluCI AATT 1 cut(s) 227
MlyI GAGTC 1 cut(s) 195
MmeI TCCRAC 2 cut(s) 199, 223
MnlI CCTC 4 cut(s) 125, 179, 182, 221
MspA1I CMGCKG 1 cut(s) 281
MspI CCGG 2 cut(s) 62, 152
MspR9I CCNGG 2 cut(s) 62, 152
Mva1269I GAATGC 1 cut(s) 215
MvnI CGCG 2 cut(s) 48, 191
NciI CCSGG 2 cut(s) 62, 152
NdeII GATC 4 cut(s) 11, 84, 103, 111
NlaIII CATG 1 cut(s) 24
NlaIV GGNNCC 1 cut(s) 18
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 1 cut(s) 215
PkrI GCNGC 4 cut(s) 47, 193, 280, 283
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PspN4I GGNNCC 1 cut(s) 18
PstI CTGCAG 1 cut(s) 286
PstNI CAGNNNCTG 1 cut(s) 281
PvuII CAGCTG 1 cut(s) 281
RsaI GTAC 2 cut(s) 18, 144
RsaNI GTAC 2 cut(s) 17, 143
SatI GCNGC 4 cut(s) 46, 192, 279, 282
Sau3AI GATC 4 cut(s) 11, 84, 103, 111
SchI GAGTC 1 cut(s) 195
ScrFI CCNGG 2 cut(s) 62, 152
SetI ASST 5 cut(s) 84, 99, 201, 237, 283
SfcI CTRYAG 1 cut(s) 282
Sse9I AATT 1 cut(s) 227
SsiI CCGC 4 cut(s) 43, 46, 148, 191
StyD4I CCNGG 2 cut(s) 60, 150
TaqI TCGA 2 cut(s) 106, 162
TasI AATT 1 cut(s) 227
TauI GCSGC 2 cut(s) 48, 194
TseI GCWGC 2 cut(s) 278, 281
TspGWI ACGGA 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.