Rh4CG100300

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0019366
Physical Location & Seq
Reverse (-)
18470191 .. 18495499
25309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG100300.1

Sequence Viewer

Length: 528 bp
ATGGAGGTGGAGTTCGACACGCTCATGGACGTGGAGTTCAAGGACATTAATAGCAACCATGTGGGCCTAGATCTCAACTCCATGGTTTCATCACAGGTCGGCGATCTTGGCGCTGTGGATATTGATCTCAAGAGCAGGGACCTCGTGAATTGCTGGATAGAATTCGGCAGGCCTGTAACAGCCATGCCATTACATATTTTCCACATTATGACACCCTTGGAGCTAAGGATGAAGCCGTGTTATGTAGTTTTTGTGGGTCTCTCAACTGGTACTAAAGCTTGTATGTACAAAGTTTTTCAAGTAAGTGTTGTGCGTAATAGAGTTTTTCTGTATCTCAAAAACCGCAAAATTTTTGACCCCAGTTATTTTAGACTTGGAAATCTTGATAGGGGTGTTGGTGTTCTTAAGCAACTTTATTCTGTCGTCTTTTCGGCATCGAAAAGACAAAATCACCTACTTAGCAGTGATTTACGAAGGAGAAGATTGATAAGATCAAGAGGCTGGACATCCCAACTTCCAGATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lectin_legB Pkinase

Protein Analysis

175

Amino Acids

20.15

Weight (kDa)

9.54

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 2 - 62 1.9e-13 Legume lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019366)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0160941
rosa_roxburghii Rroxscaffold_4G00303790
rosa_rugosa Rorug04G0341000
rosa_samantha Rh2BG456900 Rh2DG466600 Rh4CG100300 Rh5BG327200 Rh5DG338300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 343
AcsI RAATTY 2 cut(s) 161, 348
AfaI GTAC 2 cut(s) 271, 287
AflII CTTAAG 1 cut(s) 404
AgsI TTSAA 2 cut(s) 40, 299
AjiI CACGTC 1 cut(s) 31
AloI GAACNNNNNNTCC 3 cut(s) 20, 28, 52
AluBI AGCT 2 cut(s) 223, 278
AluI AGCT 2 cut(s) 223, 278
Alw26I GTCTC 1 cut(s) 263
AoxI GGCC 2 cut(s) 64, 170
ApoI RAATTY 2 cut(s) 161, 348
AseI ATTAAT 1 cut(s) 48
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 2 cut(s) 64, 139
AsuHPI GGTGA 1 cut(s) 443
AvaII GGWCC 1 cut(s) 139
BauI CACGAG 1 cut(s) 143
BceAI ACGGC 1 cut(s) 220
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BcoDI GTCTC 1 cut(s) 263
BfaI CTAG 1 cut(s) 68
BfoI RGCGCY 1 cut(s) 114
BfrI CTTAAG 1 cut(s) 404
BglII AGATCT 1 cut(s) 70
Bme18I GGWCC 1 cut(s) 139
BmgBI CACGTC 1 cut(s) 31
BmgT120I GGNCC 2 cut(s) 64, 139
BmiI GGNNCC 1 cut(s) 140
BmrI ACTGGG 1 cut(s) 354
BmsI GCATC 1 cut(s) 443
BmuI ACTGGG 1 cut(s) 354
Bpu10I CCTNAGC 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 113
BsaBI GATNNNNATC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 2 cut(s) 81, 216
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse1I ACTGG 2 cut(s) 271, 360
Bse8I GATNNNNATC 1 cut(s) 123
BseDI CCNNGG 2 cut(s) 81, 216
BseGI GGATG 2 cut(s) 234, 506
BseJI GATNNNNATC 1 cut(s) 123
BseNI ACTGG 2 cut(s) 271, 360
BshFI GGCC 2 cut(s) 66, 172
BslFI GGGAC 1 cut(s) 152
BsmAI GTCTC 1 cut(s) 263
BsmFI GGGAC 1 cut(s) 152
BsnI GGCC 2 cut(s) 66, 172
Bso31I GGTCTC 1 cut(s) 263
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 4 cut(s) 70, 103, 124, 491
Bsp19I CCATGG 1 cut(s) 81
BspACI CCGC 1 cut(s) 343
BspANI GGCC 2 cut(s) 66, 172
BspLI GGNNCC 1 cut(s) 140
BspTI CTTAAG 1 cut(s) 404
BspTNI GGTCTC 1 cut(s) 263
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 2 cut(s) 271, 360
BssECI CCNNGG 2 cut(s) 81, 216
BssMI GATC 4 cut(s) 70, 103, 124, 491
BssSI CACGAG 1 cut(s) 143
BssT1I CCWWGG 2 cut(s) 81, 216
Bst2BI CACGAG 1 cut(s) 143
BstAFI CTTAAG 1 cut(s) 404
BstAUI TGTACA 1 cut(s) 285
BstC8I GCNNGC 1 cut(s) 170
BstDEI CTNAG 2 cut(s) 224, 458
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 2 cut(s) 234, 506
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 4 cut(s) 73, 106, 127, 494
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 4 cut(s) 70, 103, 124, 491
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BsuRI GGCC 2 cut(s) 66, 172
BtgI CCRYGG 1 cut(s) 81
BtrI CACGTC 1 cut(s) 31
BtsCI GGATG 2 cut(s) 234, 506
BtsI GCAGTG 1 cut(s) 469
BtsIMutI CAGTG 1 cut(s) 469
Cac8I GCNNGC 1 cut(s) 170
CfoI GCGC 1 cut(s) 113
Cfr13I GGNCC 2 cut(s) 64, 139
Csp6I GTAC 2 cut(s) 270, 286
CviAII CATG 4 cut(s) 25, 59, 82, 184
CviJI RGCY 7 cut(s) 66, 172, 182, 223, 235, 278, 501
CviKI_1 RGCY 7 cut(s) 66, 172, 182, 223, 235, 278, 501
CviQI GTAC 2 cut(s) 270, 286
DdeI CTNAG 2 cut(s) 224, 458
DpnI GATC 4 cut(s) 72, 105, 126, 493
DpnII GATC 4 cut(s) 70, 103, 124, 491
Eco130I CCWWGG 2 cut(s) 81, 216
Eco147I AGGCCT 1 cut(s) 172
Eco31I GGTCTC 1 cut(s) 263
Eco47I GGWCC 1 cut(s) 139
EcoO109I RGGNCCY 1 cut(s) 139
EcoRI GAATTC 1 cut(s) 161
EcoT14I CCWWGG 2 cut(s) 81, 216
ErhI CCWWGG 2 cut(s) 81, 216
FaeI CATG 4 cut(s) 28, 62, 85, 187
FaiI YATR 8 cut(s) 26, 60, 83, 185, 195, 209, 243, 284
FaqI GGGAC 1 cut(s) 152
FatI CATG 4 cut(s) 24, 58, 81, 183
FokI GGATG 2 cut(s) 241, 493
FspBI CTAG 1 cut(s) 68
GlaI GCGC 1 cut(s) 112
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 2 cut(s) 66, 172
HhaI GCGC 1 cut(s) 113
Hin1II CATG 4 cut(s) 28, 62, 85, 187
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HindIII AAGCTT 1 cut(s) 276
HphI GGTGA 1 cut(s) 443
Hpy188III TCNNGA 5 cut(s) 130, 145, 383, 495, 518
HpyAV CCTTC 1 cut(s) 468
HpyCH4IV ACGT 1 cut(s) 30
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HpyF3I CTNAG 2 cut(s) 224, 458
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 4 cut(s) 28, 62, 85, 187
HspAI GCGC 1 cut(s) 111
Kzo9I GATC 4 cut(s) 70, 103, 124, 491
LmnI GCTCC 1 cut(s) 220
LpnPI CCDG 8 cut(s) 80, 121, 139, 154, 186, 252, 373, 487
LweI GCATC 1 cut(s) 443
MaeI CTAG 1 cut(s) 68
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 1 cut(s) 175
MalI GATC 4 cut(s) 72, 105, 126, 493
MboI GATC 4 cut(s) 70, 103, 124, 491
MboII GAAGA 1 cut(s) 492
MflI RGATCY 1 cut(s) 70
MluCI AATT 4 cut(s) 148, 161, 348, 523
MnlI CCTC 2 cut(s) 152, 491
MseI TTAA 2 cut(s) 48, 405
MslI CAYNNNNRTG 2 cut(s) 23, 29
MspCI CTTAAG 1 cut(s) 404
MwoI GCNNNNNNNGC 1 cut(s) 108
NcoI CCATGG 1 cut(s) 81
NdeII GATC 4 cut(s) 70, 103, 124, 491
NlaIII CATG 4 cut(s) 28, 62, 85, 187
NlaIV GGNNCC 1 cut(s) 140
PceI AGGCCT 1 cut(s) 172
PpuMI RGGWCCY 1 cut(s) 139
PshBI ATTAAT 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 139
PspN4I GGNNCC 1 cut(s) 140
PspPI GGNCC 2 cut(s) 64, 139
PspPPI RGGWCCY 1 cut(s) 139
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 2 cut(s) 271, 287
RsaNI GTAC 2 cut(s) 270, 286
RseI CAYNNNNRTG 2 cut(s) 23, 29
SaqAI TTAA 2 cut(s) 48, 405
Sau3AI GATC 4 cut(s) 70, 103, 124, 491
Sau96I GGNCC 2 cut(s) 64, 139
SetI ASST 7 cut(s) 9, 33, 99, 144, 225, 280, 456
SfaNI GCATC 1 cut(s) 443
SinI GGWCC 1 cut(s) 139
SmiMI CAYNNNNRTG 2 cut(s) 23, 29
SmlI CTYRAG 2 cut(s) 128, 404
SmoI CTYRAG 2 cut(s) 128, 404
Sse9I AATT 4 cut(s) 148, 161, 348, 523
SseBI AGGCCT 1 cut(s) 172
SsiI CCGC 1 cut(s) 343
SspMI CTAG 1 cut(s) 68
StuI AGGCCT 1 cut(s) 172
StyI CCWWGG 2 cut(s) 81, 216
TaiI ACGT 1 cut(s) 33
TaqI TCGA 2 cut(s) 15, 437
TasI AATT 4 cut(s) 148, 161, 348, 523
TatI WGTACW 1 cut(s) 285
Tru1I TTAA 2 cut(s) 48, 405
Tru9I TTAA 2 cut(s) 48, 405
TscAI CASTG 1 cut(s) 469
TspDTI ATGAA 2 cut(s) 78, 245
TspRI CASTG 1 cut(s) 469
Vha464I CTTAAG 1 cut(s) 404
VpaK11BI GGWCC 1 cut(s) 139
VspI ATTAAT 1 cut(s) 48
XapI RAATTY 2 cut(s) 161, 348
XspI CTAG 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.