Rh4DG267900

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0020146
Physical Location & Seq
Reverse (-)
48740654 .. 48742909
2256 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG267900.1

Sequence Viewer

Length: 1134 bp
ATGTCAGAGACCCATTTCAACAAGGGTGACCAAGTCGAGGTCACAACGTCCGTCCACGGCGGCTCCACCGGCCCCTACTACCCAGCCACCGTGCTCCGTCCAGTCATCAAAGACAAGGCCCGCATCCTAGTCCAGTACCAAACCCTAACGGCCCACGGCGGAGGCACAAAGCCCTTGAAGGAGATTGTGGAGGTCGGCAACGTGCGGCCCATGCCTCCCCGGGAGCTCCACCGGCCGTTCAAGGTCGGCGATGACGTGGACGCGTTCAGGGCAAAAGGGTGGTATCGTGGGACGGTGAGGGAGATTTTGGAGAATTCAAAGTACTTGGTGGTGTTGGATGGTAAAGCTGACCTGGAATTTGAGCGTGACCATTTTGATTTGAGGGTTCATAGAGAGTGGGATGATGGGTCTTGGGTCCCTCCTCTTCCACAACAGCAAAAATCATCTGGGGTGGTGAAATGCAGAAAGATAACGTTTAAAATAGAGGATAAGAAGGAGACTATGGGAGCAAACTTTGGAAGGGGGACTCTGGAGGATGATGAAGATGATAAGGAATTTGAGTCAGAGCAGTTTAATTTGAGGCTTCACAGAGAGTGGGATGACCGGTCTTCAGTCCCTCCTCTTCCGGAAGAGCAAAGGTCATCTGGGGTGGTGAAATGCAGAAAGATAAGATTCAAATTAAAGGCCAAGAAGGAAACTGTGAGGAGAAACTTTGAAAACGGGACTCTGGTGGAGGTCAGCAGTGATGAGGAGGGTTACAAGGATGCTTGGTACACTGCTAGAATAGTTGACTACATTGGAAAAGACAAGTTTCTGGTGGAATACGTGTCCCTGGTAACTGATGATAGAACCCAGCTCTTGAGAGAAGAAGCATCTTCAAGTCACATTAGGCCTTGTCCTCCTCCACTTCCACCACTTGTCCAATTCAAAGTTCTTCAGAAAGTCGATGCTTGGTACAATGAGGGATGGTGGGAAGGATCCATTTCCAAAGTTCTGACCGGCTCAAAGTACGTGGTGTATTTCAGCTCCACCAATGAGGAATTAGAGTTCAAGCATTCCAATTTGAGGCTTCATCAGGACTGGTGTAACGGGCATTGGGTAATGGATCCATGCCAGGACTTCTACGGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

377

Amino Acids

43.6

Weight (kDa)

6.17

Isoelectric Point (pI)

32.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 9 - 73 1.6e-10 Agenet domain
Agenet PF05641 241 - 301 5.4e-19 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020146)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0426121
rosa_multiflora Rmu_sc0014286.1_g000005
rosa_roxburghii Rroxscaffold_5G00368110
rosa_samantha Rh4CG284700 Rh4DG267900
rosa_wichuraiana Rw4G022960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 263
AccIII TCCGGA 1 cut(s) 625
AciI CCGC 4 cut(s) 60, 121, 159, 205
AclI AACGTT 1 cut(s) 473
AclWI GGATC 4 cut(s) 972, 985, 1100, 1113
AcoI YGGCCR 1 cut(s) 233
AcsI RAATTY 3 cut(s) 313, 356, 554
AcuI CTGAAG 2 cut(s) 594, 920
AfaI GTAC 5 cut(s) 137, 323, 773, 956, 1010
AfiI CCNNNNNNNGG 2 cut(s) 37, 1065
AflIII ACRYGT 2 cut(s) 261, 825
AgeI ACCGGT 1 cut(s) 603
AgsI TTSAA 9 cut(s) 19, 178, 241, 318, 676, 716, 879, 928, 1051
AjiI CACGTC 1 cut(s) 256
AjnI CCWGG 3 cut(s) 351, 831, 1113
AjuI GAANNNNNNNTTGG 2 cut(s) 915, 947
AloI GAACNNNNNNTCC 2 cut(s) 1031, 1063
AluBI AGCT 4 cut(s) 226, 347, 856, 1026
AluI AGCT 4 cut(s) 226, 347, 856, 1026
Alw21I GWGCWC 2 cut(s) 96, 228
Alw26I GTCTC 2 cut(s) 2, 491
AlwI GGATC 4 cut(s) 972, 985, 1100, 1113
Ama87I CYCGRG 1 cut(s) 219
Aor13HI TCCGGA 1 cut(s) 625
AoxI GGCC 7 cut(s) 70, 117, 150, 206, 233, 684, 890
ApoI RAATTY 3 cut(s) 313, 356, 554
ArsI GACNNNNNNTTYG 2 cut(s) 341, 373
AsiGI ACCGGT 1 cut(s) 603
AspS9I GGNCC 5 cut(s) 71, 118, 151, 207, 415
AsuC2I CCSGG 2 cut(s) 220, 221
AsuHPI GGTGA 4 cut(s) 38, 307, 466, 664
AvaI CYCGRG 1 cut(s) 219
AvaII GGWCC 1 cut(s) 415
BamHI GGATCC 2 cut(s) 977, 1105
BanII GRGCYC 1 cut(s) 228
BbsI GAAGAC 1 cut(s) 600
Bbv12I GWGCWC 2 cut(s) 96, 228
BccI CCATC 3 cut(s) 332, 398, 960
BceAI ACGGC 4 cut(s) 73, 165, 172, 220
BciT130I CCWGG 3 cut(s) 353, 833, 1115
BcnI CCSGG 2 cut(s) 220, 221
BcoDI GTCTC 2 cut(s) 2, 491
BfaI CTAG 2 cut(s) 128, 780
BisI GCNGC 2 cut(s) 61, 206
BlsI GCNGC 2 cut(s) 62, 207
BmcAI AGTACT 1 cut(s) 323
Bme1390I CCNGG 5 cut(s) 220, 221, 353, 833, 1115
Bme18I GGWCC 1 cut(s) 415
BmeT110I CYCGRG 1 cut(s) 219
BmgBI CACGTC 1 cut(s) 256
BmgT120I GGNCC 5 cut(s) 71, 118, 151, 207, 415
BmiI GGNNCC 6 cut(s) 64, 73, 416, 417, 979, 1107
BmrFI CCNGG 5 cut(s) 220, 221, 353, 833, 1115
BmsI GCATC 4 cut(s) 132, 754, 881, 937
BpiI GAAGAC 1 cut(s) 600
BpmI CTGGAG 1 cut(s) 551
BpuEI CTTGAG 1 cut(s) 880
BpuMI CCSGG 2 cut(s) 220, 221
BsaAI YACGTR 2 cut(s) 826, 1012
BsaI GGTCTC 1 cut(s) 2
BsaJI CCNNGG 5 cut(s) 55, 154, 218, 219, 831
BsaWI WCCGGW 2 cut(s) 603, 625
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 1065
Bse118I RCCGGY 4 cut(s) 68, 231, 603, 998
Bse1I ACTGG 3 cut(s) 101, 133, 1085
BseAI TCCGGA 1 cut(s) 625
BseBI CCWGG 3 cut(s) 353, 833, 1115
BseDI CCNNGG 5 cut(s) 55, 154, 218, 219, 831
BseGI GGATG 7 cut(s) 123, 343, 406, 541, 604, 769, 971
BseLI CCNNNNNNNGG 2 cut(s) 37, 1065
BseNI ACTGG 3 cut(s) 101, 133, 1085
BseRI GAGGAG 5 cut(s) 411, 609, 718, 764, 891
BseX3I CGGCCG 1 cut(s) 233
BseYI CCCAGC 2 cut(s) 82, 852
Bsh1236I CGCG 1 cut(s) 263
Bsh1285I CGRYCG 1 cut(s) 236
BshFI GGCC 7 cut(s) 72, 119, 152, 208, 235, 686, 892
BshTI ACCGGT 1 cut(s) 603
BsiEI CGRYCG 1 cut(s) 236
BsiHKAI GWGCWC 2 cut(s) 96, 228
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 6 cut(s) 69, 220, 232, 604, 626, 999
BslFI GGGAC 6 cut(s) 304, 401, 538, 599, 736, 814
BslI CCNNNNNNNGG 2 cut(s) 37, 1065
BsmAI GTCTC 2 cut(s) 2, 491
BsmFI GGGAC 6 cut(s) 304, 401, 538, 599, 736, 814
BsmI GAATGC 1 cut(s) 1054
BsnI GGCC 7 cut(s) 72, 119, 152, 208, 235, 686, 892
Bso31I GGTCTC 1 cut(s) 2
BsoBI CYCGRG 1 cut(s) 219
Bsp1286I GDGCHC 2 cut(s) 96, 228
Bsp13I TCCGGA 1 cut(s) 625
Bsp143I GATC 2 cut(s) 977, 1105
BspACI CCGC 4 cut(s) 60, 121, 159, 205
BspANI GGCC 7 cut(s) 72, 119, 152, 208, 235, 686, 892
BspEI TCCGGA 1 cut(s) 625
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 6 cut(s) 64, 73, 416, 417, 979, 1107
BspPI GGATC 4 cut(s) 972, 985, 1100, 1113
BspQI GCTCTTC 1 cut(s) 624
BspTNI GGTCTC 1 cut(s) 2
BsrFI RCCGGY 4 cut(s) 68, 231, 603, 998
BsrI ACTGG 3 cut(s) 101, 133, 1085
BssAI RCCGGY 4 cut(s) 68, 231, 603, 998
BssECI CCNNGG 5 cut(s) 55, 154, 218, 219, 831
BssMI GATC 2 cut(s) 977, 1105
Bst2UI CCWGG 3 cut(s) 353, 833, 1115
Bst4CI ACNGT 3 cut(s) 91, 295, 700
Bst6I CTCTTC 3 cut(s) 429, 624, 627
BstBAI YACGTR 2 cut(s) 826, 1012
BstC8I GCNNGC 1 cut(s) 121
BstDSI CCRYGG 2 cut(s) 55, 154
BstEII GGTNACC 1 cut(s) 26
BstF5I GGATG 7 cut(s) 123, 343, 406, 541, 604, 769, 971
BstFNI CGCG 1 cut(s) 263
BstKTI GATC 2 cut(s) 980, 1108
BstMAI GTCTC 2 cut(s) 2, 491
BstMBI GATC 2 cut(s) 977, 1105
BstMCI CGRYCG 1 cut(s) 236
BstMWI GCNNNNNNNGC 4 cut(s) 69, 211, 232, 269
BstNI CCWGG 3 cut(s) 353, 833, 1115
BstPI GGTNACC 1 cut(s) 26
BstSCI CCNGG 5 cut(s) 218, 219, 351, 831, 1113
BstUI CGCG 1 cut(s) 263
BstV2I GAAGAC 1 cut(s) 600
BstX2I RGATCY 2 cut(s) 977, 1105
BstYI RGATCY 2 cut(s) 977, 1105
BstZI CGGCCG 1 cut(s) 233
BsuRI GGCC 7 cut(s) 72, 119, 152, 208, 235, 686, 892
BtgI CCRYGG 2 cut(s) 55, 154
BtgZI GCGATG 1 cut(s) 264
BtrI CACGTC 1 cut(s) 256
BtsCI GGATG 7 cut(s) 123, 343, 406, 541, 604, 769, 971
BtsI GCAGTG 2 cut(s) 748, 774
BtsIMutI CAGTG 2 cut(s) 748, 774
Cac8I GCNNGC 1 cut(s) 121
Cfr10I RCCGGY 4 cut(s) 68, 231, 603, 998
Cfr13I GGNCC 5 cut(s) 71, 118, 151, 207, 415
Cfr9I CCCGGG 1 cut(s) 219
CseI GACGC 1 cut(s) 269
Csp6I GTAC 5 cut(s) 136, 322, 772, 955, 1009
CspAI ACCGGT 1 cut(s) 603
CspCI CAANNNNNGTGG 2 cut(s) 993, 1028
CviAII CATG 2 cut(s) 211, 1110
CviQI GTAC 5 cut(s) 136, 322, 772, 955, 1009
DpnI GATC 2 cut(s) 979, 1107
DpnII GATC 2 cut(s) 977, 1105
DraI TTTAAA 1 cut(s) 478
EaeI YGGCCR 1 cut(s) 233
EagI CGGCCG 1 cut(s) 233
Eam1104I CTCTTC 3 cut(s) 429, 624, 627
EarI CTCTTC 3 cut(s) 429, 624, 627
EciI GGCGGA 1 cut(s) 174
Ecl136II GAGCTC 1 cut(s) 226
EclXI CGGCCG 1 cut(s) 233
Eco147I AGGCCT 1 cut(s) 892
Eco24I GRGCYC 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 2
Eco47I GGWCC 1 cut(s) 415
Eco52I CGGCCG 1 cut(s) 233
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 2 cut(s) 594, 920
Eco88I CYCGRG 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 415
EcoO65I GGTNACC 1 cut(s) 26
EcoRI GAATTC 1 cut(s) 313
EcoRII CCWGG 3 cut(s) 351, 831, 1113
EcoT38I GRGCYC 1 cut(s) 228
FaeI CATG 2 cut(s) 214, 1113
FaiI YATR 4 cut(s) 212, 390, 503, 1111
FaqI GGGAC 6 cut(s) 304, 401, 538, 599, 736, 814
FatI CATG 2 cut(s) 210, 1109
FauI CCCGC 1 cut(s) 128
Fnu4HI GCNGC 2 cut(s) 61, 206
FokI GGATG 7 cut(s) 110, 350, 413, 548, 611, 776, 978
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 2 cut(s) 61, 206
FspBI CTAG 2 cut(s) 128, 780
GluI GCNGC 2 cut(s) 61, 206
GsaI CCCAGC 2 cut(s) 86, 856
GsuI CTGGAG 1 cut(s) 551
HaeIII GGCC 7 cut(s) 72, 119, 152, 208, 235, 686, 892
HapII CCGG 6 cut(s) 69, 220, 232, 604, 626, 999
HgaI GACGC 1 cut(s) 269
Hin1II CATG 2 cut(s) 214, 1113
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HinfI GANTC 4 cut(s) 526, 560, 672, 724
HpaII CCGG 6 cut(s) 69, 220, 232, 604, 626, 999
HphI GGTGA 4 cut(s) 38, 307, 466, 664
Hpy166II GTNNAC 4 cut(s) 55, 259, 774, 790
Hpy188I TCNGA 4 cut(s) 7, 565, 939, 996
Hpy188III TCNNGA 4 cut(s) 530, 626, 859, 1076
Hpy8I GTNNAC 4 cut(s) 55, 259, 774, 790
HpyAV CCTTC 5 cut(s) 172, 487, 513, 685, 968
HpyCH4III ACNGT 3 cut(s) 91, 295, 700
HpyCH4IV ACGT 6 cut(s) 47, 201, 255, 473, 825, 1011
HpyCH4V TGCA 2 cut(s) 462, 660
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 211, 232, 269
HpySE526I ACGT 6 cut(s) 47, 201, 255, 473, 825, 1011
Hsp92II CATG 2 cut(s) 214, 1113
KflI GGGWCCC 1 cut(s) 415
Kpn2I TCCGGA 1 cut(s) 625
Kzo9I GATC 2 cut(s) 977, 1105
LguI GCTCTTC 1 cut(s) 624
LmnI GCTCC 6 cut(s) 68, 99, 223, 231, 506, 1031
LweI GCATC 4 cut(s) 132, 754, 881, 937
MaeI CTAG 2 cut(s) 128, 780
MaeII ACGT 6 cut(s) 47, 201, 255, 473, 825, 1011
MaeIII GTNAC 7 cut(s) 26, 40, 365, 755, 835, 881, 1085
MalI GATC 2 cut(s) 979, 1107
MboI GATC 2 cut(s) 977, 1105
MboII GAAGA 8 cut(s) 416, 554, 600, 614, 641, 867, 878, 926
MflI RGATCY 2 cut(s) 977, 1105
MhlI GDGCHC 2 cut(s) 96, 228
MluCI AATT 8 cut(s) 313, 356, 554, 574, 677, 923, 1040, 1060
MluI ACGCGT 1 cut(s) 261
MlyI GAGTC 3 cut(s) 520, 569, 718
MmeI TCCRAC 1 cut(s) 315
MroI TCCGGA 1 cut(s) 625
MseI TTAA 3 cut(s) 477, 573, 680
MspI CCGG 6 cut(s) 69, 220, 232, 604, 626, 999
MspR9I CCNGG 5 cut(s) 220, 221, 353, 833, 1115
Mva1269I GAATGC 1 cut(s) 1054
MvaI CCWGG 3 cut(s) 353, 833, 1115
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 4 cut(s) 69, 211, 232, 269
NciI CCSGG 2 cut(s) 220, 221
NdeII GATC 2 cut(s) 977, 1105
NlaIII CATG 2 cut(s) 214, 1113
NlaIV GGNNCC 6 cut(s) 64, 73, 416, 417, 979, 1107
NmuCI GTSAC 4 cut(s) 26, 40, 365, 881
PceI AGGCCT 1 cut(s) 892
PciSI GCTCTTC 1 cut(s) 624
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 1 cut(s) 1054
PfeI GAWTC 1 cut(s) 672
PflFI GACNNNGTC 1 cut(s) 32
PinAI ACCGGT 1 cut(s) 603
PkrI GCNGC 2 cut(s) 62, 207
PleI GAGTC 3 cut(s) 520, 568, 718
PpsI GAGTC 3 cut(s) 520, 568, 718
Ppu21I YACGTR 2 cut(s) 826, 1012
PpuMI RGGWCCY 1 cut(s) 415
Psp124BI GAGCTC 1 cut(s) 228
Psp1406I AACGTT 1 cut(s) 473
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 3 cut(s) 351, 831, 1113
PspEI GGTNACC 1 cut(s) 26
PspFI CCCAGC 2 cut(s) 82, 852
PspGI CCWGG 3 cut(s) 351, 831, 1113
PspN4I GGNNCC 6 cut(s) 64, 73, 416, 417, 979, 1107
PspPI GGNCC 5 cut(s) 71, 118, 151, 207, 415
PspPPI RGGWCCY 1 cut(s) 415
PsuI RGATCY 2 cut(s) 977, 1105
PsyI GACNNNGTC 1 cut(s) 32
RsaI GTAC 5 cut(s) 137, 323, 773, 956, 1010
RsaNI GTAC 5 cut(s) 136, 322, 772, 955, 1009
SacI GAGCTC 1 cut(s) 228
SapI GCTCTTC 1 cut(s) 624
SaqAI TTAA 3 cut(s) 477, 573, 680
SatI GCNGC 2 cut(s) 61, 206
Sau3AI GATC 2 cut(s) 977, 1105
Sau96I GGNCC 5 cut(s) 71, 118, 151, 207, 415
ScaI AGTACT 1 cut(s) 323
SchI GAGTC 3 cut(s) 520, 569, 718
ScrFI CCNGG 5 cut(s) 220, 221, 353, 833, 1115
SduI GDGCHC 2 cut(s) 96, 228
SfaNI GCATC 4 cut(s) 132, 754, 881, 937
SinI GGWCC 1 cut(s) 415
SmaI CCCGGG 1 cut(s) 221
SmlI CTYRAG 1 cut(s) 859
SmoI CTYRAG 1 cut(s) 859
Sse9I AATT 8 cut(s) 313, 356, 554, 574, 677, 923, 1040, 1060
SseBI AGGCCT 1 cut(s) 892
SsiI CCGC 4 cut(s) 60, 121, 159, 205
SspMI CTAG 2 cut(s) 128, 780
SstI GAGCTC 1 cut(s) 228
StuI AGGCCT 1 cut(s) 892
StyD4I CCNGG 5 cut(s) 218, 219, 351, 831, 1113
TaaI ACNGT 3 cut(s) 91, 295, 700
TaiI ACGT 6 cut(s) 50, 204, 258, 476, 828, 1014
TaqI TCGA 2 cut(s) 36, 945
TasI AATT 8 cut(s) 313, 356, 554, 574, 677, 923, 1040, 1060
TatI WGTACW 1 cut(s) 321
TauI GCSGC 2 cut(s) 63, 208
TfiI GAWTC 1 cut(s) 672
Tru1I TTAA 3 cut(s) 477, 573, 680
Tru9I TTAA 3 cut(s) 477, 573, 680
TscAI CASTG 2 cut(s) 748, 781
TseFI GTSAC 4 cut(s) 26, 40, 365, 881
Tsp45I GTSAC 4 cut(s) 26, 40, 365, 881
TspDTI ATGAA 3 cut(s) 377, 555, 1061
TspGWI ACGGA 2 cut(s) 40, 86
TspMI CCCGGG 1 cut(s) 219
TspRI CASTG 2 cut(s) 748, 781
Tth111I GACNNNGTC 1 cut(s) 32
VpaK11BI GGWCC 1 cut(s) 415
XapI RAATTY 3 cut(s) 313, 356, 554
XmaI CCCGGG 1 cut(s) 219
XspI CTAG 2 cut(s) 128, 780
ZrmI AGTACT 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.