Rw4G022960

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0020146
Physical Location & Seq
Reverse (-)
48451601 .. 48453847
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G022960.1

Sequence Viewer

Length: 1143 bp
ATGTCAGAGACCCATTTCAACAAGGGTGACCAAGTCGAGGTCACAACCTCCGTCCACGGCGGCTCCACCGGCCCCTACTACCCAGCCACCGTGCTCCGTCCAGTCATCAAAGACAAGGCCCGCATCCTAGTCCAGTACCAAACCCTAATGGCCCACGGCGGAGGCACAAAGCCCTTGAAGGAGATTGTGGAGGTCGGCAACGTGCGGCCCATGCCTCCCCGGGAGCTCCACCGGCCGTTCAAGGTCGGCGATGACGTGGACGCGTTCAGGGCAAAAGGGTGGTATCGTGGGACGGTGAGGGAGATTTTGGAGAATTCAAAGTACTTGGTGGTGTTGGATGGTAAAGCTGACCTGGAATTCGAGCGTGACCATTTTGATTTGAGGGTTCATAGAGAGTGGGATGATGGCTCTTGGGTCCCTCCTCTTCCACAAGAGCAAAAATCATCTAGGGTGGTGAAATGCAGAAAGATAACGTTTAAAATAGAGGATAAGAAGGAGACTATGGGAGCAAACTTTGGAAGGGGGACTCTGGAGGATGATGAGGATGATGAAGATGATAAGGAATTCGAGTCTGAGCAGTTTAATTTGAGGCTTCACAGAGAGTGGGATGACCGGTCTTCAGTCCCTCCTCTTCCGGAAGAGCAAAGGTCATCTGGGGTGGTGAAATGCAGAAAGATAAGATTCAAATTAAAGGCCAAGAAGGAAACTGTGAGGAGAAACTTTGAAAACGGGACTCTGGTGGAGGTCAGCAGTGATGAGGAGGGTTACAAGGATGCTTGGTACACTGCTAGAATAGTTGACTACATTGGAAAAGACAAGTTTCTGGTGGAATACGTGTCCCTGGTAACTGATGATAGAACCCAGCTCTTGAGAGAAGAAGCATCTTCAAGTCACATTAGGCCTTGTCCTCCTCCACTTCCACCACTTGTCCAATTCAAAGTTCTTCAGAAAGTCGATGCTTGGTACAATGAGGGATGGTGGGAAGGATCCATTTCCAAAGTTCTGACCGGCTCAAAGTACGTGGTGTATTTCAGCTCCACCAATGAGGAATTAGAGTTCAAGCATTCCAATTTGAGGCTTCATCAGGACTGGTGTAACGGGCATTGGGTAATGGATCCATGCCAGGACTTCTATGGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet

Protein Analysis

380

Amino Acids

44.09

Weight (kDa)

5.86

Isoelectric Point (pI)

33.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 9 - 73 5.9e-11 Agenet domain
Agenet PF05641 244 - 304 5.5e-19 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020146)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0426121
rosa_multiflora Rmu_sc0014286.1_g000005
rosa_roxburghii Rroxscaffold_5G00368110
rosa_samantha Rh4CG284700 Rh4DG267900
rosa_wichuraiana Rw4G022960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 263
AccIII TCCGGA 1 cut(s) 634
AciI CCGC 4 cut(s) 60, 121, 159, 205
AclI AACGTT 1 cut(s) 473
AclWI GGATC 4 cut(s) 981, 994, 1109, 1122
AcoI YGGCCR 1 cut(s) 233
AcsI RAATTY 3 cut(s) 313, 356, 563
AcuI CTGAAG 2 cut(s) 603, 929
AfaI GTAC 5 cut(s) 137, 323, 782, 965, 1019
AfiI CCNNNNNNNGG 2 cut(s) 37, 1074
AflIII ACRYGT 2 cut(s) 261, 834
AgeI ACCGGT 1 cut(s) 612
AgsI TTSAA 9 cut(s) 19, 178, 241, 318, 685, 725, 888, 937, 1060
AjiI CACGTC 1 cut(s) 256
AjnI CCWGG 3 cut(s) 351, 840, 1122
AjuI GAANNNNNNNTTGG 2 cut(s) 924, 956
AloI GAACNNNNNNTCC 2 cut(s) 1040, 1072
AluBI AGCT 4 cut(s) 226, 347, 865, 1035
AluI AGCT 4 cut(s) 226, 347, 865, 1035
Alw21I GWGCWC 2 cut(s) 96, 228
Alw26I GTCTC 2 cut(s) 2, 491
AlwI GGATC 4 cut(s) 981, 994, 1109, 1122
Ama87I CYCGRG 1 cut(s) 219
Aor13HI TCCGGA 1 cut(s) 634
AoxI GGCC 7 cut(s) 70, 117, 150, 206, 233, 693, 899
ApoI RAATTY 3 cut(s) 313, 356, 563
ArsI GACNNNNNNTTYG 2 cut(s) 341, 373
AsiGI ACCGGT 1 cut(s) 612
AspS9I GGNCC 5 cut(s) 71, 118, 151, 207, 415
AsuC2I CCSGG 2 cut(s) 220, 221
AsuHPI GGTGA 4 cut(s) 38, 307, 466, 673
AvaI CYCGRG 1 cut(s) 219
AvaII GGWCC 1 cut(s) 415
BamHI GGATCC 2 cut(s) 986, 1114
BanII GRGCYC 1 cut(s) 228
BbsI GAAGAC 1 cut(s) 609
Bbv12I GWGCWC 2 cut(s) 96, 228
BccI CCATC 3 cut(s) 332, 398, 969
BceAI ACGGC 3 cut(s) 73, 172, 220
BciT130I CCWGG 3 cut(s) 353, 842, 1124
BcnI CCSGG 2 cut(s) 220, 221
BcoDI GTCTC 2 cut(s) 2, 491
BfaI CTAG 3 cut(s) 128, 447, 789
BisI GCNGC 2 cut(s) 61, 206
BlsI GCNGC 2 cut(s) 62, 207
BmcAI AGTACT 1 cut(s) 323
Bme1390I CCNGG 5 cut(s) 220, 221, 353, 842, 1124
Bme18I GGWCC 1 cut(s) 415
BmeT110I CYCGRG 1 cut(s) 219
BmgBI CACGTC 1 cut(s) 256
BmgT120I GGNCC 5 cut(s) 71, 118, 151, 207, 415
BmiI GGNNCC 6 cut(s) 64, 73, 416, 417, 988, 1116
BmrFI CCNGG 5 cut(s) 220, 221, 353, 842, 1124
BmsI GCATC 4 cut(s) 132, 763, 890, 946
BpiI GAAGAC 1 cut(s) 609
BpmI CTGGAG 1 cut(s) 551
BpuEI CTTGAG 1 cut(s) 889
BpuMI CCSGG 2 cut(s) 220, 221
BsaAI YACGTR 2 cut(s) 835, 1021
BsaI GGTCTC 1 cut(s) 2
BsaJI CCNNGG 5 cut(s) 55, 154, 218, 219, 840
BsaWI WCCGGW 2 cut(s) 612, 634
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 1074
Bse118I RCCGGY 4 cut(s) 68, 231, 612, 1007
Bse1I ACTGG 3 cut(s) 101, 133, 1094
BseAI TCCGGA 1 cut(s) 634
BseBI CCWGG 3 cut(s) 353, 842, 1124
BseDI CCNNGG 5 cut(s) 55, 154, 218, 219, 840
BseGI GGATG 8 cut(s) 123, 343, 406, 541, 550, 613, 778, 980
BseLI CCNNNNNNNGG 2 cut(s) 37, 1074
BseMII CTCAG 1 cut(s) 564
BseNI ACTGG 3 cut(s) 101, 133, 1094
BseRI GAGGAG 5 cut(s) 411, 618, 727, 773, 900
BseX3I CGGCCG 1 cut(s) 233
BseYI CCCAGC 2 cut(s) 82, 861
Bsh1236I CGCG 1 cut(s) 263
Bsh1285I CGRYCG 1 cut(s) 236
BshFI GGCC 7 cut(s) 72, 119, 152, 208, 235, 695, 901
BshTI ACCGGT 1 cut(s) 612
BsiEI CGRYCG 1 cut(s) 236
BsiHKAI GWGCWC 2 cut(s) 96, 228
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 6 cut(s) 69, 220, 232, 613, 635, 1008
BslFI GGGAC 6 cut(s) 304, 401, 538, 608, 745, 823
BslI CCNNNNNNNGG 2 cut(s) 37, 1074
BsmAI GTCTC 2 cut(s) 2, 491
BsmFI GGGAC 6 cut(s) 304, 401, 538, 608, 745, 823
BsmI GAATGC 1 cut(s) 1063
BsnI GGCC 7 cut(s) 72, 119, 152, 208, 235, 695, 901
Bso31I GGTCTC 1 cut(s) 2
BsoBI CYCGRG 1 cut(s) 219
Bsp1286I GDGCHC 2 cut(s) 96, 228
Bsp13I TCCGGA 1 cut(s) 634
Bsp143I GATC 2 cut(s) 986, 1114
BspACI CCGC 4 cut(s) 60, 121, 159, 205
BspANI GGCC 7 cut(s) 72, 119, 152, 208, 235, 695, 901
BspCNI CTCAG 1 cut(s) 565
BspEI TCCGGA 1 cut(s) 634
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 6 cut(s) 64, 73, 416, 417, 988, 1116
BspPI GGATC 4 cut(s) 981, 994, 1109, 1122
BspQI GCTCTTC 1 cut(s) 633
BspTNI GGTCTC 1 cut(s) 2
BsrFI RCCGGY 4 cut(s) 68, 231, 612, 1007
BsrI ACTGG 3 cut(s) 101, 133, 1094
BssAI RCCGGY 4 cut(s) 68, 231, 612, 1007
BssECI CCNNGG 5 cut(s) 55, 154, 218, 219, 840
BssMI GATC 2 cut(s) 986, 1114
Bst2UI CCWGG 3 cut(s) 353, 842, 1124
Bst4CI ACNGT 3 cut(s) 91, 295, 709
Bst6I CTCTTC 3 cut(s) 429, 633, 636
BstBAI YACGTR 2 cut(s) 835, 1021
BstC8I GCNNGC 1 cut(s) 121
BstDEI CTNAG 1 cut(s) 573
BstDSI CCRYGG 2 cut(s) 55, 154
BstEII GGTNACC 1 cut(s) 26
BstF5I GGATG 8 cut(s) 123, 343, 406, 541, 550, 613, 778, 980
BstFNI CGCG 1 cut(s) 263
BstKTI GATC 2 cut(s) 989, 1117
BstMAI GTCTC 2 cut(s) 2, 491
BstMBI GATC 2 cut(s) 986, 1114
BstMCI CGRYCG 1 cut(s) 236
BstMWI GCNNNNNNNGC 4 cut(s) 69, 211, 232, 269
BstNI CCWGG 3 cut(s) 353, 842, 1124
BstPI GGTNACC 1 cut(s) 26
BstSCI CCNGG 5 cut(s) 218, 219, 351, 840, 1122
BstUI CGCG 1 cut(s) 263
BstV2I GAAGAC 1 cut(s) 609
BstX2I RGATCY 2 cut(s) 986, 1114
BstYI RGATCY 2 cut(s) 986, 1114
BstZI CGGCCG 1 cut(s) 233
BsuRI GGCC 7 cut(s) 72, 119, 152, 208, 235, 695, 901
BtgI CCRYGG 2 cut(s) 55, 154
BtgZI GCGATG 1 cut(s) 264
BtrI CACGTC 1 cut(s) 256
BtsCI GGATG 8 cut(s) 123, 343, 406, 541, 550, 613, 778, 980
BtsI GCAGTG 2 cut(s) 757, 783
BtsIMutI CAGTG 2 cut(s) 757, 783
Cac8I GCNNGC 1 cut(s) 121
Cfr10I RCCGGY 4 cut(s) 68, 231, 612, 1007
Cfr13I GGNCC 5 cut(s) 71, 118, 151, 207, 415
Cfr9I CCCGGG 1 cut(s) 219
CseI GACGC 1 cut(s) 269
Csp6I GTAC 5 cut(s) 136, 322, 781, 964, 1018
CspAI ACCGGT 1 cut(s) 612
CspCI CAANNNNNGTGG 2 cut(s) 1002, 1037
CviAII CATG 2 cut(s) 211, 1119
CviQI GTAC 5 cut(s) 136, 322, 781, 964, 1018
DdeI CTNAG 1 cut(s) 573
DpnI GATC 2 cut(s) 988, 1116
DpnII GATC 2 cut(s) 986, 1114
DraI TTTAAA 1 cut(s) 478
EaeI YGGCCR 1 cut(s) 233
EagI CGGCCG 1 cut(s) 233
Eam1104I CTCTTC 3 cut(s) 429, 633, 636
EarI CTCTTC 3 cut(s) 429, 633, 636
EciI GGCGGA 1 cut(s) 174
Ecl136II GAGCTC 1 cut(s) 226
EclXI CGGCCG 1 cut(s) 233
Eco147I AGGCCT 1 cut(s) 901
Eco24I GRGCYC 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 2
Eco47I GGWCC 1 cut(s) 415
Eco52I CGGCCG 1 cut(s) 233
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 2 cut(s) 603, 929
Eco88I CYCGRG 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 415
EcoO65I GGTNACC 1 cut(s) 26
EcoRI GAATTC 3 cut(s) 313, 356, 563
EcoRII CCWGG 3 cut(s) 351, 840, 1122
EcoT38I GRGCYC 1 cut(s) 228
FaeI CATG 2 cut(s) 214, 1122
FaiI YATR 5 cut(s) 212, 390, 503, 1120, 1134
FaqI GGGAC 6 cut(s) 304, 401, 538, 608, 745, 823
FatI CATG 2 cut(s) 210, 1118
FauI CCCGC 1 cut(s) 128
Fnu4HI GCNGC 2 cut(s) 61, 206
FokI GGATG 8 cut(s) 110, 350, 413, 548, 557, 620, 785, 987
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 2 cut(s) 61, 206
FspBI CTAG 3 cut(s) 128, 447, 789
GluI GCNGC 2 cut(s) 61, 206
GsaI CCCAGC 2 cut(s) 86, 865
GsuI CTGGAG 1 cut(s) 551
HaeIII GGCC 7 cut(s) 72, 119, 152, 208, 235, 695, 901
HapII CCGG 6 cut(s) 69, 220, 232, 613, 635, 1008
HgaI GACGC 1 cut(s) 269
Hin1II CATG 2 cut(s) 214, 1122
HincII GTYRAC 1 cut(s) 799
HindII GTYRAC 1 cut(s) 799
HinfI GANTC 4 cut(s) 526, 569, 681, 733
HpaII CCGG 6 cut(s) 69, 220, 232, 613, 635, 1008
HphI GGTGA 4 cut(s) 38, 307, 466, 673
Hpy166II GTNNAC 4 cut(s) 55, 259, 783, 799
Hpy188I TCNGA 4 cut(s) 7, 574, 948, 1005
Hpy188III TCNNGA 4 cut(s) 530, 635, 868, 1085
Hpy8I GTNNAC 4 cut(s) 55, 259, 783, 799
HpyAV CCTTC 5 cut(s) 172, 487, 513, 694, 977
HpyCH4III ACNGT 3 cut(s) 91, 295, 709
HpyCH4IV ACGT 5 cut(s) 201, 255, 473, 834, 1020
HpyCH4V TGCA 2 cut(s) 462, 669
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 211, 232, 269
HpyF3I CTNAG 1 cut(s) 573
HpySE526I ACGT 5 cut(s) 201, 255, 473, 834, 1020
Hsp92II CATG 2 cut(s) 214, 1122
KflI GGGWCCC 1 cut(s) 415
Kpn2I TCCGGA 1 cut(s) 634
Kzo9I GATC 2 cut(s) 986, 1114
LguI GCTCTTC 1 cut(s) 633
LmnI GCTCC 6 cut(s) 68, 99, 223, 231, 506, 1040
LweI GCATC 4 cut(s) 132, 763, 890, 946
MaeI CTAG 3 cut(s) 128, 447, 789
MaeII ACGT 5 cut(s) 201, 255, 473, 834, 1020
MaeIII GTNAC 7 cut(s) 26, 40, 365, 764, 844, 890, 1094
MalI GATC 2 cut(s) 988, 1116
MboI GATC 2 cut(s) 986, 1114
MboII GAAGA 8 cut(s) 416, 563, 609, 623, 650, 876, 887, 935
MflI RGATCY 2 cut(s) 986, 1114
MhlI GDGCHC 2 cut(s) 96, 228
MluCI AATT 8 cut(s) 313, 356, 563, 583, 686, 932, 1049, 1069
MluI ACGCGT 1 cut(s) 261
MlyI GAGTC 3 cut(s) 520, 578, 727
MmeI TCCRAC 1 cut(s) 315
MroI TCCGGA 1 cut(s) 634
MseI TTAA 3 cut(s) 477, 582, 689
MspI CCGG 6 cut(s) 69, 220, 232, 613, 635, 1008
MspR9I CCNGG 5 cut(s) 220, 221, 353, 842, 1124
Mva1269I GAATGC 1 cut(s) 1063
MvaI CCWGG 3 cut(s) 353, 842, 1124
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 4 cut(s) 69, 211, 232, 269
NciI CCSGG 2 cut(s) 220, 221
NdeII GATC 2 cut(s) 986, 1114
NlaIII CATG 2 cut(s) 214, 1122
NlaIV GGNNCC 6 cut(s) 64, 73, 416, 417, 988, 1116
NmuCI GTSAC 4 cut(s) 26, 40, 365, 890
PceI AGGCCT 1 cut(s) 901
PciSI GCTCTTC 1 cut(s) 633
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 1 cut(s) 1063
PfeI GAWTC 1 cut(s) 681
PflFI GACNNNGTC 1 cut(s) 32
PinAI ACCGGT 1 cut(s) 612
PkrI GCNGC 2 cut(s) 62, 207
PleI GAGTC 3 cut(s) 520, 577, 727
PpsI GAGTC 3 cut(s) 520, 577, 727
Ppu21I YACGTR 2 cut(s) 835, 1021
PpuMI RGGWCCY 1 cut(s) 415
Psp124BI GAGCTC 1 cut(s) 228
Psp1406I AACGTT 1 cut(s) 473
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 3 cut(s) 351, 840, 1122
PspEI GGTNACC 1 cut(s) 26
PspFI CCCAGC 2 cut(s) 82, 861
PspGI CCWGG 3 cut(s) 351, 840, 1122
PspN4I GGNNCC 6 cut(s) 64, 73, 416, 417, 988, 1116
PspPI GGNCC 5 cut(s) 71, 118, 151, 207, 415
PspPPI RGGWCCY 1 cut(s) 415
PsuI RGATCY 2 cut(s) 986, 1114
PsyI GACNNNGTC 1 cut(s) 32
RsaI GTAC 5 cut(s) 137, 323, 782, 965, 1019
RsaNI GTAC 5 cut(s) 136, 322, 781, 964, 1018
SacI GAGCTC 1 cut(s) 228
SapI GCTCTTC 1 cut(s) 633
SaqAI TTAA 3 cut(s) 477, 582, 689
SatI GCNGC 2 cut(s) 61, 206
Sau3AI GATC 2 cut(s) 986, 1114
Sau96I GGNCC 5 cut(s) 71, 118, 151, 207, 415
ScaI AGTACT 1 cut(s) 323
SchI GAGTC 3 cut(s) 520, 578, 727
ScrFI CCNGG 5 cut(s) 220, 221, 353, 842, 1124
SduI GDGCHC 2 cut(s) 96, 228
SfaNI GCATC 4 cut(s) 132, 763, 890, 946
SinI GGWCC 1 cut(s) 415
SmaI CCCGGG 1 cut(s) 221
SmlI CTYRAG 1 cut(s) 868
SmoI CTYRAG 1 cut(s) 868
Sse9I AATT 8 cut(s) 313, 356, 563, 583, 686, 932, 1049, 1069
SseBI AGGCCT 1 cut(s) 901
SsiI CCGC 4 cut(s) 60, 121, 159, 205
SspMI CTAG 3 cut(s) 128, 447, 789
SstI GAGCTC 1 cut(s) 228
StuI AGGCCT 1 cut(s) 901
StyD4I CCNGG 5 cut(s) 218, 219, 351, 840, 1122
TaaI ACNGT 3 cut(s) 91, 295, 709
TaiI ACGT 5 cut(s) 204, 258, 476, 837, 1023
TaqI TCGA 4 cut(s) 36, 360, 567, 954
TasI AATT 8 cut(s) 313, 356, 563, 583, 686, 932, 1049, 1069
TatI WGTACW 1 cut(s) 321
TauI GCSGC 2 cut(s) 63, 208
TfiI GAWTC 1 cut(s) 681
Tru1I TTAA 3 cut(s) 477, 582, 689
Tru9I TTAA 3 cut(s) 477, 582, 689
TscAI CASTG 2 cut(s) 757, 790
TseFI GTSAC 4 cut(s) 26, 40, 365, 890
Tsp45I GTSAC 4 cut(s) 26, 40, 365, 890
TspDTI ATGAA 3 cut(s) 377, 564, 1070
TspGWI ACGGA 2 cut(s) 40, 86
TspMI CCCGGG 1 cut(s) 219
TspRI CASTG 2 cut(s) 757, 790
Tth111I GACNNNGTC 1 cut(s) 32
VpaK11BI GGWCC 1 cut(s) 415
XapI RAATTY 3 cut(s) 313, 356, 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 1130
XmaI CCCGGG 1 cut(s) 219
XspI CTAG 3 cut(s) 128, 447, 789
ZrmI AGTACT 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.