Rh4DG296700

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0025700
Physical Location & Seq
Forward (+)
51870028 .. 51871721
1694 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG296700.1

Sequence Viewer

Length: 1098 bp
ATGAGTTTCTCTCGGTTCTATTTTCAGGATTATATCCACCGCGATTATCAATTTGACTATTTTTCAATTTCTGACACTGAAAAATATGAAATTCCAAAATGGTTCCGTCGGAGTAGTGGCTCTTCTATAAGAATTCCATTACCTCCAGATTTCGACTGCAACAGTAGATGGATGGGAGTGGCTCTGTTTGTTTCTTATGAATTGCATGGCAATGTGGATGAAACTGAAAACCAACGGGAATGGGAAGAGGCAAGGGAAGGCATATTAAGGAGCAGTTACCAATACAGTTGTCATATCTACACAAATGATGATGATTCTCTTGAAAGTCCCCTGGTGCGTAGACTAAATAATTGCCGTATTGTTGGACCTGGATCATACCAATATTGGTTCTACATACCAAGACGGGAGTTAGCAAAAAGGTTGAATAAAGGCTCCATCCGAGCTGAAATAATACCAGAGGCGAATGTGAAAGAGTGTGGGGCGAGACTAATATACTCTGACGATGTGCCCAACTTTATTCAGAGTCTAGTCCAGCCATACAGTCCCGCGGACCATGATGTTATTGTTGATTATAAGTTGCGAAACCCAGACAAAAGTATCAGCACAAAGTGCTTCTTCTCTGGACCTAGTGGCATTTTCCAAAAAGAAAAGAACAGTACTGATCAGCTGGAACTACTTAAACAAGTTGCAGAGCTAATTCCTTCTCATGAACCAACTCCATTCTTCATTGAAATTGAACCTCCCGGATATGATTCAAGTTCAACCAAGATTGTCGGTGAACTGAGAAGAAACATAGAGTTAGTTCTTCACCAACTCTTTGAGGGAGGCAATGCAGGTTACTATGATTATGGTTTCACTGTATCTCCTAGAGTCAAAGCAATTTTTCCATGGTTTAGTGATCAAAGCGTTGGGAGCGAAGTACGACTACCTCATTCTCCTCCGGATTTGTACAAGGACAAGACATGGCTAGGATTATCTGTCTATGCTGCCTTCATTGTGTCTCCAGGTTCAACATTTTCCTGTGCTTGTTACTTGATTACCGTCACTGGAGAATATTTGGTCGCTTTCAACCTTCCCACAGCCTTCAGGGTTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

365

Amino Acids

42.09

Weight (kDa)

5.48

Isoelectric Point (pI)

57.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 29 - 169 4e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025700)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8449195.1_g000001
rosa_samantha Rh4BG299700 Rh4DG296700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 573
Acc36I ACCTGC 1 cut(s) 824
AccI GTMKAC 1 cut(s) 340
AccII CGCG 2 cut(s) 42, 548
AccIII TCCGGA 1 cut(s) 940
AciI CCGC 3 cut(s) 40, 546, 548
AclWI GGATC 1 cut(s) 379
AcsI RAATTY 2 cut(s) 90, 132
AcuI CTGAAG 1 cut(s) 1069
AdeI CACNNNGTG 1 cut(s) 609
AfaI GTAC 3 cut(s) 658, 921, 950
AgsI TTSAA 9 cut(s) 66, 323, 424, 731, 737, 756, 762, 1011, 1069
AjnI CCWGG 3 cut(s) 330, 367, 1003
AluBI AGCT 3 cut(s) 443, 667, 694
AluI AGCT 3 cut(s) 443, 667, 694
Alw26I GTCTC 2 cut(s) 478, 1005
AlwI GGATC 1 cut(s) 379
Aor13HI TCCGGA 1 cut(s) 940
ApeKI GCWGC 1 cut(s) 986
ApoI RAATTY 2 cut(s) 90, 132
AspS9I GGNCC 3 cut(s) 365, 550, 623
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 2 cut(s) 788, 800
AvaII GGWCC 3 cut(s) 365, 550, 623
BaeGI GKGCMC 1 cut(s) 510
BaeI ACNNNNGTAYC 2 cut(s) 580, 613
BarI GAAGNNNNNNTAC 4 cut(s) 106, 138, 909, 941
BbvI GCAGC 1 cut(s) 973
BccI CCATC 3 cut(s) 162, 166, 443
BceAI ACGGC 1 cut(s) 339
BciT130I CCWGG 3 cut(s) 332, 369, 1005
BclI TGATCA 2 cut(s) 661, 898
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 2 cut(s) 478, 1005
BfaI CTAG 4 cut(s) 527, 627, 867, 968
BfuAI ACCTGC 1 cut(s) 824
BisI GCNGC 1 cut(s) 987
BlsI GCNGC 1 cut(s) 988
BmcAI AGTACT 1 cut(s) 658
Bme1390I CCNGG 4 cut(s) 332, 369, 744, 1005
Bme18I GGWCC 3 cut(s) 365, 550, 623
BmgT120I GGNCC 3 cut(s) 365, 550, 623
BmiI GGNNCC 2 cut(s) 104, 433
BmrFI CCNGG 4 cut(s) 332, 369, 744, 1005
BplI GAGNNNNNCTC 1 cut(s) 27
BpmI CTGGAG 3 cut(s) 129, 987, 1068
BpuMI CCSGG 1 cut(s) 744
BsaJI CCNNGG 3 cut(s) 330, 546, 887
BsaWI WCCGGW 1 cut(s) 940
BsaXI ACNNNNNCTCC 2 cut(s) 847, 877
Bse1I ACTGG 1 cut(s) 1051
Bse3DI GCAATG 2 cut(s) 217, 835
BseAI TCCGGA 1 cut(s) 940
BseBI CCWGG 3 cut(s) 332, 369, 1005
BseDI CCNNGG 3 cut(s) 330, 546, 887
BseGI GGATG 3 cut(s) 177, 223, 435
BseMI GCAATG 2 cut(s) 217, 835
BseMII CTCAG 1 cut(s) 773
BseNI ACTGG 1 cut(s) 1051
BseRI GAGGAG 1 cut(s) 927
BseSI GKGCMC 1 cut(s) 510
BseXI GCAGC 1 cut(s) 973
Bsh1236I CGCG 2 cut(s) 42, 548
BsiSI CCGG 2 cut(s) 744, 941
BslFI GGGAC 2 cut(s) 312, 528
BsmAI GTCTC 2 cut(s) 478, 1005
BsmFI GGGAC 2 cut(s) 312, 528
Bsp1286I GDGCHC 1 cut(s) 510
Bsp13I TCCGGA 1 cut(s) 940
Bsp1407I TGTACA 1 cut(s) 948
Bsp143I GATC 3 cut(s) 371, 661, 898
Bsp19I CCATGG 1 cut(s) 887
BspACI CCGC 3 cut(s) 40, 546, 548
BspCNI CTCAG 1 cut(s) 774
BspEI TCCGGA 1 cut(s) 940
BspFNI CGCG 2 cut(s) 42, 548
BspHI TCATGA 1 cut(s) 706
BspLI GGNNCC 2 cut(s) 104, 433
BspMI ACCTGC 1 cut(s) 824
BspPI GGATC 1 cut(s) 379
BspQI GCTCTTC 1 cut(s) 127
BsrDI GCAATG 2 cut(s) 217, 835
BsrGI TGTACA 1 cut(s) 948
BsrI ACTGG 1 cut(s) 1051
BssECI CCNNGG 3 cut(s) 330, 546, 887
BssMI GATC 3 cut(s) 371, 661, 898
BssT1I CCWWGG 1 cut(s) 887
Bst2UI CCWGG 3 cut(s) 332, 369, 1005
Bst4CI ACNGT 6 cut(s) 164, 287, 542, 656, 859, 1042
Bst6I CTCTTC 2 cut(s) 127, 240
BstAPI GCANNNNNTGC 1 cut(s) 609
BstAUI TGTACA 1 cut(s) 948
BstDEI CTNAG 1 cut(s) 782
BstDSI CCRYGG 2 cut(s) 546, 887
BstF5I GGATG 3 cut(s) 177, 223, 435
BstFNI CGCG 2 cut(s) 42, 548
BstKTI GATC 3 cut(s) 374, 664, 901
BstMAI GTCTC 2 cut(s) 478, 1005
BstMBI GATC 3 cut(s) 371, 661, 898
BstMWI GCNNNNNNNGC 2 cut(s) 609, 912
BstNI CCWGG 3 cut(s) 332, 369, 1005
BstSCI CCNGG 4 cut(s) 330, 367, 742, 1003
BstSLI GKGCMC 1 cut(s) 510
BstUI CGCG 2 cut(s) 42, 548
BstV1I GCAGC 1 cut(s) 973
BtgI CCRYGG 2 cut(s) 546, 887
BtsCI GGATG 3 cut(s) 177, 223, 435
BtsIMutI CAGTG 3 cut(s) 75, 855, 1044
BveI ACCTGC 1 cut(s) 824
CciI TCATGA 1 cut(s) 706
Cfr13I GGNCC 3 cut(s) 365, 550, 623
Cfr42I CCGCGG 1 cut(s) 549
Csp6I GTAC 3 cut(s) 657, 920, 949
CviAII CATG 5 cut(s) 206, 554, 707, 888, 963
CviJI RGCY 9 cut(s) 120, 182, 432, 443, 535, 667, 694, 967, 1082
CviKI_1 RGCY 9 cut(s) 120, 182, 432, 443, 535, 667, 694, 967, 1082
CviQI GTAC 3 cut(s) 657, 920, 949
DdeI CTNAG 1 cut(s) 782
DpnI GATC 3 cut(s) 373, 663, 900
DpnII GATC 3 cut(s) 371, 661, 898
DraIII CACNNNGTG 1 cut(s) 609
Eam1104I CTCTTC 2 cut(s) 127, 240
EarI CTCTTC 2 cut(s) 127, 240
Eco130I CCWWGG 1 cut(s) 887
Eco47I GGWCC 3 cut(s) 365, 550, 623
Eco57I CTGAAG 1 cut(s) 1069
EcoRI GAATTC 1 cut(s) 132
EcoRII CCWGG 3 cut(s) 330, 367, 1003
EcoT14I CCWWGG 1 cut(s) 887
ErhI CCWWGG 1 cut(s) 887
FaeI CATG 5 cut(s) 209, 557, 710, 891, 966
FalI AAGNNNNNCTT 2 cut(s) 599, 631
FaqI GGGAC 2 cut(s) 312, 528
FatI CATG 5 cut(s) 205, 553, 706, 887, 962
FauI CCCGC 1 cut(s) 553
FbaI TGATCA 2 cut(s) 661, 898
FblI GTMKAC 1 cut(s) 340
Fnu4HI GCNGC 1 cut(s) 987
FokI GGATG 3 cut(s) 184, 230, 422
Fsp4HI GCNGC 1 cut(s) 987
FspBI CTAG 4 cut(s) 527, 627, 867, 968
GluI GCNGC 1 cut(s) 987
GsuI CTGGAG 3 cut(s) 129, 987, 1068
HapII CCGG 2 cut(s) 744, 941
Hin1II CATG 5 cut(s) 209, 557, 710, 891, 966
HinfI GANTC 4 cut(s) 314, 523, 752, 870
HpaII CCGG 2 cut(s) 744, 941
HphI GGTGA 2 cut(s) 788, 800
Hpy166II GTNNAC 2 cut(s) 341, 779
Hpy188I TCNGA 5 cut(s) 73, 111, 440, 499, 522
Hpy188III TCNNGA 6 cut(s) 26, 146, 320, 621, 707, 941
Hpy8I GTNNAC 2 cut(s) 341, 779
Hpy99I CGWCG 1 cut(s) 111
HpyAV CCTTC 5 cut(s) 251, 711, 1000, 1082, 1093
HpyCH4III ACNGT 6 cut(s) 164, 287, 542, 656, 859, 1042
HpyCH4V TGCA 4 cut(s) 159, 205, 689, 833
HpyF10VI GCNNNNNNNGC 2 cut(s) 609, 912
HpyF3I CTNAG 1 cut(s) 782
Hsp92II CATG 5 cut(s) 209, 557, 710, 891, 966
Kpn2I TCCGGA 1 cut(s) 940
Ksp22I TGATCA 2 cut(s) 661, 898
KspI CCGCGG 1 cut(s) 549
Kzo9I GATC 3 cut(s) 371, 661, 898
LguI GCTCTTC 1 cut(s) 127
LmnI GCTCC 3 cut(s) 270, 437, 912
Lsp1109I GCAGC 1 cut(s) 973
MaeI CTAG 4 cut(s) 527, 627, 867, 968
MaeIII GTNAC 4 cut(s) 275, 836, 1028, 1042
MalI GATC 3 cut(s) 373, 663, 900
MboI GATC 3 cut(s) 371, 661, 898
MboII GAAGA 6 cut(s) 114, 257, 607, 715, 797, 798
MhlI GDGCHC 1 cut(s) 510
MluCI AATT 9 cut(s) 50, 66, 90, 132, 200, 349, 696, 732, 879
MlyI GAGTC 2 cut(s) 532, 879
MmeI TCCRAC 2 cut(s) 89, 343
MnlI CCTC 8 cut(s) 153, 241, 451, 750, 814, 818, 939, 948
MroI TCCGGA 1 cut(s) 940
MseI TTAA 2 cut(s) 266, 678
MslI CAYNNNNRTG 1 cut(s) 210
MspA1I CMGCKG 2 cut(s) 548, 667
MspI CCGG 2 cut(s) 744, 941
MspR9I CCNGG 4 cut(s) 332, 369, 744, 1005
MvaI CCWGG 3 cut(s) 332, 369, 1005
MvnI CGCG 2 cut(s) 42, 548
MwoI GCNNNNNNNGC 2 cut(s) 609, 912
NciI CCSGG 1 cut(s) 744
NcoI CCATGG 1 cut(s) 887
NdeII GATC 3 cut(s) 371, 661, 898
NlaIII CATG 5 cut(s) 209, 557, 710, 891, 966
NlaIV GGNNCC 2 cut(s) 104, 433
NmuCI GTSAC 1 cut(s) 1042
PagI TCATGA 1 cut(s) 706
PciSI GCTCTTC 1 cut(s) 127
PfeI GAWTC 2 cut(s) 314, 752
PfoI TCCNGGA 1 cut(s) 742
PkrI GCNGC 1 cut(s) 988
PleI GAGTC 2 cut(s) 531, 878
PpsI GAGTC 2 cut(s) 531, 878
PsiI TTATAA 1 cut(s) 573
Psp6I CCWGG 3 cut(s) 330, 367, 1003
PspGI CCWGG 3 cut(s) 330, 367, 1003
PspN4I GGNNCC 2 cut(s) 104, 433
PspPI GGNCC 3 cut(s) 365, 550, 623
PvuII CAGCTG 1 cut(s) 667
RsaI GTAC 3 cut(s) 658, 921, 950
RsaNI GTAC 3 cut(s) 657, 920, 949
RseI CAYNNNNRTG 1 cut(s) 210
SacII CCGCGG 1 cut(s) 549
SapI GCTCTTC 1 cut(s) 127
SaqAI TTAA 2 cut(s) 266, 678
SatI GCNGC 1 cut(s) 987
Sau3AI GATC 3 cut(s) 371, 661, 898
Sau96I GGNCC 3 cut(s) 365, 550, 623
ScaI AGTACT 1 cut(s) 658
SchI GAGTC 2 cut(s) 532, 879
ScrFI CCNGG 4 cut(s) 332, 369, 744, 1005
SduI GDGCHC 1 cut(s) 510
Sfr303I CCGCGG 1 cut(s) 549
SgrBI CCGCGG 1 cut(s) 549
SinI GGWCC 3 cut(s) 365, 550, 623
SmiMI CAYNNNNRTG 1 cut(s) 210
Sse9I AATT 9 cut(s) 50, 66, 90, 132, 200, 349, 696, 732, 879
SsiI CCGC 3 cut(s) 40, 546, 548
SspI AATATT 2 cut(s) 383, 1055
SspMI CTAG 4 cut(s) 527, 627, 867, 968
StyD4I CCNGG 4 cut(s) 330, 367, 742, 1003
StyI CCWWGG 1 cut(s) 887
TaaI ACNGT 6 cut(s) 164, 287, 542, 656, 859, 1042
TaqI TCGA 1 cut(s) 153
TasI AATT 9 cut(s) 50, 66, 90, 132, 200, 349, 696, 732, 879
TatI WGTACW 2 cut(s) 656, 948
TfiI GAWTC 2 cut(s) 314, 752
Tru1I TTAA 2 cut(s) 266, 678
Tru9I TTAA 2 cut(s) 266, 678
TscAI CASTG 3 cut(s) 82, 862, 1051
TseFI GTSAC 1 cut(s) 1042
TseI GCWGC 1 cut(s) 986
Tsp45I GTSAC 1 cut(s) 1042
TspDTI ATGAA 7 cut(s) 102, 213, 234, 715, 723, 982, 1082
TspGWI ACGGA 1 cut(s) 95
TspRI CASTG 3 cut(s) 82, 862, 1051
VpaK11BI GGWCC 3 cut(s) 365, 550, 623
XapI RAATTY 2 cut(s) 90, 132
XmiI GTMKAC 1 cut(s) 340
XspI CTAG 4 cut(s) 527, 627, 867, 968
ZrmI AGTACT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.