Rh4DG355200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0019228
Physical Location & Seq
Forward (+)
57677932 .. 57680778
2847 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG355200.1

Sequence Viewer

Length: 555 bp
ATGAAGCTTCGGGTTTCCAACACTAACGTTGGTTGCTTCATTATAAAGTCACAGACATTTCAAGTGCGATTAGGGAACACTATATCATCCATAATTGTGTTCCTGATACTTGTTCTCTTCCTTATTATTTCCATGGCTTCTCTAACCTCTCTTATTATTCAAACTAGGATTTGCAGCTGCTGGGCAGCTCAGAGTCAGTGGAAGCCAAAAGCATCAGATTACTTGCCAGTTTCTATTACTGATTGCCATATTGAGTTGCACAGTAACCAACCGAAGACTCCACTAAGTTTGGGGAAGACAATCCTCAGTCTCACATTTCAAGCCATTGTCAGCTGCGCTCTTGCTTTTCAAACCAACAATTCATCACACATTTCACCGGCTTGGCACATTGTTGGGGTGGCCGTGGCAATAGCTTTTGCAGCTTGTTTCTCTGGAATTTTCTTAAGGCATTCCTACCCAAATGCTGCAACTATCATTGAGAAAACTGGGTGTGCTTCGGCCGCAATCGCGTTTTTCCTAATGACGAGTATTTTTCTCCCTAGCAATATCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

19.94

Weight (kDa)

9.28

Isoelectric Point (pI)

47.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019228)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g28851
rosa_chinensis RchiOBHm_Chr4g0436961
rosa_laevigata RLG00000006480
rosa_rugosa Rorug04G0297700
rosa_samantha Rh4AG352000 Rh4BG361100 Rh4CG375400 Rh4DG355200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 44
AccII CGCG 1 cut(s) 509
AciI CCGC 1 cut(s) 501
AclI AACGTT 1 cut(s) 27
AcoI YGGCCR 2 cut(s) 399, 498
AcsI RAATTY 1 cut(s) 435
AflII CTTAAG 1 cut(s) 442
AgsI TTSAA 4 cut(s) 62, 161, 320, 350
AluBI AGCT 6 cut(s) 7, 177, 188, 333, 413, 422
AluI AGCT 6 cut(s) 7, 177, 188, 333, 413, 422
Alw26I GTCTC 1 cut(s) 314
AlwNI CAGNNNCTG 1 cut(s) 180
AoxI GGCC 2 cut(s) 399, 498
ApeKI GCWGC 6 cut(s) 174, 177, 185, 333, 419, 464
ApoI RAATTY 1 cut(s) 435
AspLEI GCGC 1 cut(s) 338
AsuHPI GGTGA 1 cut(s) 366
BbsI GAAGAC 2 cut(s) 281, 302
BbvI GCAGC 6 cut(s) 164, 186, 197, 320, 431, 451
BceAI ACGGC 1 cut(s) 386
BcoDI GTCTC 1 cut(s) 314
BfaI CTAG 2 cut(s) 165, 540
BfrI CTTAAG 1 cut(s) 442
BisI GCNGC 7 cut(s) 175, 178, 186, 334, 420, 465, 501
BlsI GCNGC 7 cut(s) 176, 179, 187, 335, 421, 466, 502
BmrI ACTGGG 1 cut(s) 495
BmsI GCATC 1 cut(s) 221
BmuI ACTGGG 1 cut(s) 495
BpiI GAAGAC 2 cut(s) 281, 302
BsaJI CCNNGG 2 cut(s) 132, 402
Bse118I RCCGGY 1 cut(s) 376
Bse1I ACTGG 2 cut(s) 227, 490
BseDI CCNNGG 2 cut(s) 132, 402
BseGI GGATG 1 cut(s) 86
BseMII CTCAG 2 cut(s) 203, 319
BseNI ACTGG 2 cut(s) 227, 490
BseX3I CGGCCG 1 cut(s) 498
BseXI GCAGC 6 cut(s) 164, 186, 197, 320, 431, 451
BseYI CCCAGC 1 cut(s) 180
Bsh1236I CGCG 1 cut(s) 509
Bsh1285I CGRYCG 1 cut(s) 501
BshFI GGCC 2 cut(s) 401, 500
BsiEI CGRYCG 1 cut(s) 501
BsiSI CCGG 1 cut(s) 377
BsmAI GTCTC 1 cut(s) 314
BsmI GAATGC 1 cut(s) 448
BsnI GGCC 2 cut(s) 401, 500
Bsp19I CCATGG 1 cut(s) 132
BspACI CCGC 1 cut(s) 501
BspANI GGCC 2 cut(s) 401, 500
BspCNI CTCAG 2 cut(s) 202, 318
BspFNI CGCG 1 cut(s) 509
BspHI TCATGA 1 cut(s) 551
BspTI CTTAAG 1 cut(s) 442
BsrFI RCCGGY 1 cut(s) 376
BsrI ACTGG 2 cut(s) 227, 490
BssAI RCCGGY 1 cut(s) 376
BssECI CCNNGG 2 cut(s) 132, 402
BssT1I CCWWGG 1 cut(s) 132
Bst4CI ACNGT 1 cut(s) 263
Bst6I CTCTTC 1 cut(s) 122
BstAFI CTTAAG 1 cut(s) 442
BstDEI CTNAG 3 cut(s) 189, 284, 305
BstDSI CCRYGG 2 cut(s) 132, 402
BstF5I GGATG 1 cut(s) 86
BstFNI CGCG 1 cut(s) 509
BstHHI GCGC 1 cut(s) 338
BstMAI GTCTC 1 cut(s) 314
BstMCI CGRYCG 1 cut(s) 501
BstMWI GCNNNNNNNGC 3 cut(s) 419, 500, 506
BstUI CGCG 1 cut(s) 509
BstV1I GCAGC 6 cut(s) 164, 186, 197, 320, 431, 451
BstV2I GAAGAC 2 cut(s) 281, 302
BstZI CGGCCG 1 cut(s) 498
BsuRI GGCC 2 cut(s) 401, 500
BtgI CCRYGG 2 cut(s) 132, 402
BtsCI GGATG 1 cut(s) 86
BtsIMutI CAGTG 1 cut(s) 203
CaiI CAGNNNCTG 1 cut(s) 180
CciI TCATGA 1 cut(s) 551
CfoI GCGC 1 cut(s) 338
Cfr10I RCCGGY 1 cut(s) 376
CspCI CAANNNNNGTGG 2 cut(s) 270, 305
CviAII CATG 2 cut(s) 133, 552
DdeI CTNAG 3 cut(s) 189, 284, 305
EaeI YGGCCR 2 cut(s) 399, 498
EagI CGGCCG 1 cut(s) 498
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
EclXI CGGCCG 1 cut(s) 498
Eco130I CCWWGG 1 cut(s) 132
Eco52I CGGCCG 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 2 cut(s) 136, 555
FaiI YATR 6 cut(s) 44, 83, 92, 134, 249, 553
FatI CATG 2 cut(s) 132, 551
Fnu4HI GCNGC 7 cut(s) 175, 178, 186, 334, 420, 465, 501
FokI GGATG 1 cut(s) 73
Fsp4HI GCNGC 7 cut(s) 175, 178, 186, 334, 420, 465, 501
FspBI CTAG 2 cut(s) 165, 540
GlaI GCGC 1 cut(s) 337
GluI GCNGC 7 cut(s) 175, 178, 186, 334, 420, 465, 501
GsaI CCCAGC 1 cut(s) 184
HaeIII GGCC 2 cut(s) 401, 500
HapII CCGG 1 cut(s) 377
HhaI GCGC 1 cut(s) 338
Hin1II CATG 2 cut(s) 136, 555
Hin6I GCGC 1 cut(s) 336
HinP1I GCGC 1 cut(s) 336
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 2 cut(s) 193, 277
HpaII CCGG 1 cut(s) 377
HphI GGTGA 1 cut(s) 366
Hpy188I TCNGA 2 cut(s) 192, 217
Hpy188III TCNNGA 3 cut(s) 103, 432, 552
HpyCH4III ACNGT 1 cut(s) 263
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 4 cut(s) 174, 259, 419, 467
HpyF10VI GCNNNNNNNGC 3 cut(s) 419, 500, 506
HpyF3I CTNAG 3 cut(s) 189, 284, 305
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 2 cut(s) 136, 555
HspAI GCGC 1 cut(s) 336
LpnPI CCDG 6 cut(s) 116, 166, 240, 390, 417, 471
Lsp1109I GCAGC 6 cut(s) 164, 186, 197, 320, 431, 451
LweI GCATC 1 cut(s) 221
MaeI CTAG 2 cut(s) 165, 540
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 2 cut(s) 48, 263
MboII GAAGA 3 cut(s) 109, 286, 307
MluCI AATT 3 cut(s) 93, 358, 435
MlyI GAGTC 2 cut(s) 202, 271
MmeI TCCRAC 1 cut(s) 42
MnlI CCTC 2 cut(s) 157, 314
MseI TTAA 1 cut(s) 443
MslI CAYNNNNRTG 1 cut(s) 95
MspA1I CMGCKG 2 cut(s) 177, 333
MspCI CTTAAG 1 cut(s) 442
MspI CCGG 1 cut(s) 377
Mva1269I GAATGC 1 cut(s) 448
MvnI CGCG 1 cut(s) 509
MwoI GCNNNNNNNGC 3 cut(s) 419, 500, 506
NcoI CCATGG 1 cut(s) 132
NlaIII CATG 2 cut(s) 136, 555
NmuCI GTSAC 1 cut(s) 48
PagI TCATGA 1 cut(s) 551
PctI GAATGC 1 cut(s) 448
PkrI GCNGC 7 cut(s) 176, 179, 187, 335, 421, 466, 502
PleI GAGTC 2 cut(s) 201, 271
PpsI GAGTC 2 cut(s) 201, 271
PsiI TTATAA 1 cut(s) 44
Psp1406I AACGTT 1 cut(s) 27
PspFI CCCAGC 1 cut(s) 180
PstNI CAGNNNCTG 1 cut(s) 180
PvuII CAGCTG 2 cut(s) 177, 333
RseI CAYNNNNRTG 1 cut(s) 95
SaqAI TTAA 1 cut(s) 443
SatI GCNGC 7 cut(s) 175, 178, 186, 334, 420, 465, 501
SchI GAGTC 2 cut(s) 202, 271
SetI ASST 8 cut(s) 9, 30, 149, 179, 190, 335, 415, 424
SfaNI GCATC 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 95
SmlI CTYRAG 1 cut(s) 442
SmoI CTYRAG 1 cut(s) 442
Sse9I AATT 3 cut(s) 93, 358, 435
SsiI CCGC 1 cut(s) 501
SspMI CTAG 2 cut(s) 165, 540
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 1 cut(s) 263
TaiI ACGT 1 cut(s) 30
TasI AATT 3 cut(s) 93, 358, 435
TauI GCSGC 1 cut(s) 503
Tru1I TTAA 1 cut(s) 443
Tru9I TTAA 1 cut(s) 443
TscAI CASTG 1 cut(s) 203
TseFI GTSAC 1 cut(s) 48
TseI GCWGC 6 cut(s) 174, 177, 185, 333, 419, 464
Tsp45I GTSAC 1 cut(s) 48
TspDTI ATGAA 3 cut(s) 17, 28, 351
TspRI CASTG 1 cut(s) 203
Vha464I CTTAAG 1 cut(s) 442
XapI RAATTY 1 cut(s) 435
XspI CTAG 2 cut(s) 165, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.