Rh5AG186400

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0021638
Physical Location & Seq
Forward (+)
21358527 .. 21379786
21260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG186400.1

Sequence Viewer

Length: 681 bp
ATGGTTTCCGAGCATGAGGAGCTTGGATCAGTTCAAGTCCCTCTCCAAATCGGTCTCAGGAGGACCTCTGAAGACCTTCGCCTACCCTACTCTTCACGTCCGCCGGAGTCGACCTTGTTGGGAAGCTTGGCGAATTTGAAGCTCACCGCAGATATCAGCTACAAGAAAGGTGTCAAGTTTACGAATTTTGGTGAGAAAAATCACAGGCCCGCAAGAAGTGAATCTGGTCATTTTTGCAAAACTCTCAGCAGAAATCTTGTGCATGTTTGGCTAACAATGGGAGAGAAAGGAACAGTTTGTGTAACTGGTGGTGGAGGCTTCCTGGCATCTTTGGTCATCAAGCTGCTTCTCTTTCAGGGATACAGAGTTTATGGCTCTGCTAGAGCCCCAGGTGATGAGAAGAACGCCAATTTGAAGAAAGGCGACCATCTTTTGACGAAAATATTGATCTTTTTTTATCAGTATTTGAGCAACATGTGGGGATATGGGAATGAGCTATGTGGGCGTGGAATTGGAACCACAGTAGTAGAAAGTCAGTATGTCAATGATACAGCTTCTCTGCATAAGATAGTACAGAATGTATTCAAGGGTGTTGGACCAAAGCCACTAATCTTATCACCTGGAGGGTTCTTTGATGCACTTGGTTCAAAGAACTTGCAGATAAATCCACCAGTTCATTAG

Protein Analysis

226

Amino Acids

24.78

Weight (kDa)

9.49

Isoelectric Point (pI)

31.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 155 - 213 2.5e-15 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021638)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000857.1_g000027 Rmu_sc0006599.1_g000005 Rmu_sc0010495.1_g000002
rosa_roxburghii Rroxscaffold_3G00257870
rosa_samantha Rh5AG186400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 110
AciI CCGC 3 cut(s) 101, 147, 210
AclWI GGATC 1 cut(s) 34
AcsI RAATTY 2 cut(s) 133, 184
AcuI CTGAAG 1 cut(s) 90
AfaI GTAC 1 cut(s) 573
AflIII ACRYGT 1 cut(s) 474
AgsI TTSAA 5 cut(s) 35, 139, 415, 586, 648
AjiI CACGTC 1 cut(s) 98
AjnI CCWGG 3 cut(s) 321, 388, 619
AluBI AGCT 7 cut(s) 22, 126, 142, 159, 343, 496, 554
AluI AGCT 7 cut(s) 22, 126, 142, 159, 343, 496, 554
Alw26I GTCTC 1 cut(s) 59
AlwI GGATC 1 cut(s) 34
AoxI GGCC 1 cut(s) 206
ApeKI GCWGC 1 cut(s) 343
ApoI RAATTY 2 cut(s) 133, 184
Asp700I GAANNNNTTC 2 cut(s) 75, 581
AspS9I GGNCC 3 cut(s) 63, 207, 596
AsuHPI GGTGA 4 cut(s) 136, 203, 404, 609
AvaII GGWCC 2 cut(s) 63, 596
BanII GRGCYC 1 cut(s) 388
BbsI GAAGAC 1 cut(s) 78
BbvI GCAGC 1 cut(s) 330
BccI CCATC 1 cut(s) 435
BciT130I CCWGG 3 cut(s) 323, 390, 621
BciVI GTATCC 1 cut(s) 353
BcoDI GTCTC 1 cut(s) 59
BfaI CTAG 1 cut(s) 381
BfuI GTATCC 1 cut(s) 353
BisI GCNGC 1 cut(s) 344
BlsI GCNGC 1 cut(s) 345
Bme1390I CCNGG 3 cut(s) 323, 390, 621
Bme18I GGWCC 2 cut(s) 63, 596
BmgBI CACGTC 1 cut(s) 98
BmgT120I GGNCC 3 cut(s) 63, 207, 596
BmiI GGNNCC 1 cut(s) 517
BmrFI CCNGG 3 cut(s) 323, 390, 621
BmsI GCATC 2 cut(s) 335, 625
BpiI GAAGAC 1 cut(s) 78
BpmI CTGGAG 1 cut(s) 642
BsaI GGTCTC 1 cut(s) 59
BsaJI CCNNGG 1 cut(s) 388
Bse1I ACTGG 2 cut(s) 310, 671
BseBI CCWGG 3 cut(s) 323, 390, 621
BseDI CCNNGG 1 cut(s) 388
BseMII CTCAG 2 cut(s) 70, 259
BseNI ACTGG 2 cut(s) 310, 671
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 1 cut(s) 330
BshFI GGCC 1 cut(s) 208
BsiSI CCGG 1 cut(s) 104
BslFI GGGAC 1 cut(s) 23
BsmAI GTCTC 1 cut(s) 59
BsmFI GGGAC 1 cut(s) 23
BsnI GGCC 1 cut(s) 208
Bso31I GGTCTC 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 388
Bsp143I GATC 2 cut(s) 26, 447
BspACI CCGC 3 cut(s) 101, 147, 210
BspANI GGCC 1 cut(s) 208
BspCNI CTCAG 2 cut(s) 69, 258
BspLI GGNNCC 1 cut(s) 517
BspPI GGATC 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 59
BsrI ACTGG 2 cut(s) 310, 671
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 2 cut(s) 26, 447
Bst2UI CCWGG 3 cut(s) 323, 390, 621
Bst4CI ACNGT 2 cut(s) 295, 523
Bst6I CTCTTC 1 cut(s) 97
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 2 cut(s) 56, 245
BstKTI GATC 2 cut(s) 29, 450
BstMAI GTCTC 1 cut(s) 59
BstMBI GATC 2 cut(s) 26, 447
BstMWI GCNNNNNNNGC 3 cut(s) 19, 268, 502
BstNI CCWGG 3 cut(s) 323, 390, 621
BstNSI RCATGY 2 cut(s) 266, 478
BstSCI CCNGG 3 cut(s) 321, 388, 619
BstV1I GCAGC 1 cut(s) 330
BstV2I GAAGAC 1 cut(s) 78
BsuI GTATCC 1 cut(s) 353
BsuRI GGCC 1 cut(s) 208
BtrI CACGTC 1 cut(s) 98
Cac8I GCNNGC 1 cut(s) 210
Cfr13I GGNCC 3 cut(s) 63, 207, 596
Csp6I GTAC 1 cut(s) 572
CviAII CATG 3 cut(s) 14, 263, 475
CviQI GTAC 1 cut(s) 572
DdeI CTNAG 2 cut(s) 56, 245
DpnI GATC 2 cut(s) 28, 449
DpnII GATC 2 cut(s) 26, 447
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
EciI GGCGGA 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 388
Eco31I GGTCTC 1 cut(s) 59
Eco32I GATATC 1 cut(s) 154
Eco47I GGWCC 2 cut(s) 63, 596
Eco57I CTGAAG 1 cut(s) 90
EcoO109I RGGNCCY 1 cut(s) 63
EcoRII CCWGG 3 cut(s) 321, 388, 619
EcoRV GATATC 1 cut(s) 154
EcoT38I GRGCYC 1 cut(s) 388
FaeI CATG 3 cut(s) 17, 266, 478
FaiI YATR 8 cut(s) 15, 264, 372, 476, 486, 499, 540, 564
FaqI GGGAC 1 cut(s) 23
FatI CATG 3 cut(s) 13, 262, 474
FauI CCCGC 1 cut(s) 217
FblI GTMKAC 1 cut(s) 110
Fnu4HI GCNGC 1 cut(s) 344
FriOI GRGCYC 1 cut(s) 388
Fsp4HI GCNGC 1 cut(s) 344
FspBI CTAG 1 cut(s) 381
GluI GCNGC 1 cut(s) 344
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 1 cut(s) 208
HapII CCGG 1 cut(s) 104
Hin1II CATG 3 cut(s) 17, 266, 478
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HindIII AAGCTT 1 cut(s) 124
HinfI GANTC 2 cut(s) 107, 221
HpaII CCGG 1 cut(s) 104
HphI GGTGA 4 cut(s) 136, 203, 404, 609
Hpy166II GTNNAC 2 cut(s) 111, 180
Hpy188I TCNGA 2 cut(s) 10, 70
Hpy188III TCNNGA 1 cut(s) 58
Hpy8I GTNNAC 2 cut(s) 111, 180
HpyAV CCTTC 1 cut(s) 86
HpyCH4III ACNGT 2 cut(s) 295, 523
HpyCH4IV ACGT 1 cut(s) 97
HpyCH4V TGCA 5 cut(s) 237, 262, 562, 638, 658
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 268, 502
HpyF3I CTNAG 2 cut(s) 56, 245
HpySE526I ACGT 1 cut(s) 97
Hsp92II CATG 3 cut(s) 17, 266, 478
Kzo9I GATC 2 cut(s) 26, 447
LmnI GCTCC 1 cut(s) 19
Lsp1109I GCAGC 1 cut(s) 330
LweI GCATC 2 cut(s) 335, 625
MaeI CTAG 1 cut(s) 381
MaeII ACGT 1 cut(s) 97
MaeIII GTNAC 1 cut(s) 301
MalI GATC 2 cut(s) 28, 449
MboI GATC 2 cut(s) 26, 447
MboII GAAGA 4 cut(s) 83, 84, 412, 427
MhlI GDGCHC 1 cut(s) 388
MluCI AATT 4 cut(s) 133, 184, 409, 510
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 574
MnlI CCTC 6 cut(s) 10, 51, 54, 76, 308, 617
MroXI GAANNNNTTC 2 cut(s) 75, 581
MspI CCGG 1 cut(s) 104
MspR9I CCNGG 3 cut(s) 323, 390, 621
MvaI CCWGG 3 cut(s) 323, 390, 621
MwoI GCNNNNNNNGC 3 cut(s) 19, 268, 502
NdeII GATC 2 cut(s) 26, 447
NlaIII CATG 3 cut(s) 17, 266, 478
NlaIV GGNNCC 1 cut(s) 517
NspI RCATGY 2 cut(s) 266, 478
PciI ACATGT 1 cut(s) 474
PdmI GAANNNNTTC 2 cut(s) 75, 581
PfeI GAWTC 1 cut(s) 221
PkrI GCNGC 1 cut(s) 345
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
PpuMI RGGWCCY 1 cut(s) 63
PscI ACATGT 1 cut(s) 474
Psp5II RGGWCCY 1 cut(s) 63
Psp6I CCWGG 3 cut(s) 321, 388, 619
PspGI CCWGG 3 cut(s) 321, 388, 619
PspN4I GGNNCC 1 cut(s) 517
PspPI GGNCC 3 cut(s) 63, 207, 596
PspPPI RGGWCCY 1 cut(s) 63
RsaI GTAC 1 cut(s) 573
RsaNI GTAC 1 cut(s) 572
SalI GTCGAC 1 cut(s) 109
SatI GCNGC 1 cut(s) 344
Sau3AI GATC 2 cut(s) 26, 447
Sau96I GGNCC 3 cut(s) 63, 207, 596
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 3 cut(s) 323, 390, 621
SduI GDGCHC 1 cut(s) 388
SfaNI GCATC 2 cut(s) 335, 625
SinI GGWCC 2 cut(s) 63, 596
Sse9I AATT 4 cut(s) 133, 184, 409, 510
SsiI CCGC 3 cut(s) 101, 147, 210
SspI AATATT 1 cut(s) 444
SspMI CTAG 1 cut(s) 381
StyD4I CCNGG 3 cut(s) 321, 388, 619
TaaI ACNGT 2 cut(s) 295, 523
TaiI ACGT 1 cut(s) 100
TaqI TCGA 1 cut(s) 110
TaqII GACCGA 1 cut(s) 41
TasI AATT 4 cut(s) 133, 184, 409, 510
TatI WGTACW 1 cut(s) 571
TfiI GAWTC 1 cut(s) 221
TseI GCWGC 1 cut(s) 343
TspDTI ATGAA 1 cut(s) 665
VpaK11BI GGWCC 2 cut(s) 63, 596
XapI RAATTY 2 cut(s) 133, 184
XceI RCATGY 2 cut(s) 266, 478
XmiI GTMKAC 1 cut(s) 110
XmnI GAANNNNTTC 2 cut(s) 75, 581
XspI CTAG 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.