Rh5AG232700

Homeobox-leucine zipper protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0021421
Physical Location & Seq
Forward (+)
28099915 .. 28100487
573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG232700.1

Sequence Viewer

Length: 489 bp
ATGCCAGTGTCAAGGTCAGCTGCCATCCATTGCAAGCCATGGTACAGATTGACTAGGATCACGGATGTTGCAAACAGTGATTCTCTCTTTAATGCCAAGTGTCTTGATATCAATAACAATTATTACATGCTGATGTTCCAAGAGACGAGGACGGACGCTTCAGGGTCAGCAGTTGTGTATGCACTGATTACTTCAACAAGTTCAGATATCATGATGGATGCCGTGTACGAGGGTGGTGATTCTACTTATGTAAATCTTTTACCATCAGGATTTGCAATCCTTCCTTGTGGTACATCTGACTATGCCAAAGGCGAAGGAAATGGCTCTGACGACGGTGAATGTCTGCTAACAGTTGGATTCCGAGTCGTGTGTGATGGTGGGCAACCGACTTCCAAGCTCACTATGCTGTCGGTTAAGACTAAATTGATCTCATCTCCCCCAGCATTCAGAAGATCAGAACTGGCTCTTCAAATAAATGATGGCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Homeobox START

Protein Analysis

162

Amino Acids

17.45

Weight (kDa)

4.86

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDF2_C PF25797 22 - 140 1e-22 PDF2-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021421)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0033661
rosa_multiflora Rmu_sc0004596.1_g000001 Rmu_sc0004596.1_g000027
rosa_samantha Rh5AG232700 Rh5CG262200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 65
AcuI CTGAAG 1 cut(s) 144
AfaI GTAC 3 cut(s) 44, 227, 292
AgsI TTSAA 2 cut(s) 195, 470
AluBI AGCT 2 cut(s) 20, 397
AluI AGCT 2 cut(s) 20, 397
Alw26I GTCTC 1 cut(s) 137
AlwI GGATC 1 cut(s) 65
AoxI GGCC 1 cut(s) 481
ApeKI GCWGC 1 cut(s) 20
AspS9I GGNCC 1 cut(s) 482
AsuHPI GGTGA 2 cut(s) 248, 347
BbvI GCAGC 1 cut(s) 7
BccI CCATC 5 cut(s) 32, 208, 271, 368, 473
BceAI ACGGC 1 cut(s) 206
BcoDI GTCTC 1 cut(s) 137
BfaI CTAG 1 cut(s) 54
BisI GCNGC 1 cut(s) 21
BlsI GCNGC 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 482
BmsI GCATC 1 cut(s) 208
BsaJI CCNNGG 1 cut(s) 38
Bse1I ACTGG 2 cut(s) 5, 465
Bse3DI GCAATG 1 cut(s) 28
BseDI CCNNGG 1 cut(s) 38
BseGI GGATG 3 cut(s) 24, 70, 223
BseMI GCAATG 1 cut(s) 28
BseNI ACTGG 2 cut(s) 5, 465
BseXI GCAGC 1 cut(s) 7
BseYI CCCAGC 1 cut(s) 439
BshFI GGCC 1 cut(s) 483
BsmAI GTCTC 1 cut(s) 137
BsmBI CGTCTC 1 cut(s) 137
BsmI GAATGC 1 cut(s) 443
BsnI GGCC 1 cut(s) 483
Bsp143I GATC 3 cut(s) 57, 426, 452
Bsp19I CCATGG 1 cut(s) 38
BspANI GGCC 1 cut(s) 483
BspHI TCATGA 1 cut(s) 210
BspPI GGATC 1 cut(s) 65
BspQI GCTCTTC 1 cut(s) 471
BsrDI GCAATG 1 cut(s) 28
BsrI ACTGG 2 cut(s) 5, 465
BssECI CCNNGG 1 cut(s) 38
BssMI GATC 3 cut(s) 57, 426, 452
BssT1I CCWWGG 1 cut(s) 38
Bst4CI ACNGT 3 cut(s) 77, 335, 352
Bst6I CTCTTC 1 cut(s) 471
BstC8I GCNNGC 1 cut(s) 35
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 3 cut(s) 24, 70, 223
BstKTI GATC 3 cut(s) 60, 429, 455
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 3 cut(s) 57, 426, 452
BstMWI GCNNNNNNNGC 1 cut(s) 403
BstNSI RCATGY 1 cut(s) 130
BstV1I GCAGC 1 cut(s) 7
BsuRI GGCC 1 cut(s) 483
BtgI CCRYGG 1 cut(s) 38
BtsCI GGATG 3 cut(s) 24, 70, 223
BtsIMutI CAGTG 3 cut(s) 12, 82, 182
Cac8I GCNNGC 1 cut(s) 35
CciI TCATGA 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 482
CseI GACGC 1 cut(s) 164
Csp6I GTAC 3 cut(s) 43, 226, 291
CviAII CATG 3 cut(s) 39, 127, 211
CviJI RGCY 6 cut(s) 20, 37, 324, 397, 464, 483
CviKI_1 RGCY 6 cut(s) 20, 37, 324, 397, 464, 483
CviQI GTAC 3 cut(s) 43, 226, 291
DpnI GATC 3 cut(s) 59, 428, 454
DpnII GATC 3 cut(s) 57, 426, 452
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 38
Eco32I GATATC 2 cut(s) 109, 208
Eco57I CTGAAG 1 cut(s) 144
EcoRV GATATC 2 cut(s) 109, 208
EcoT14I CCWWGG 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 38
Esp3I CGTCTC 1 cut(s) 137
FaeI CATG 3 cut(s) 42, 130, 214
FaiI YATR 7 cut(s) 40, 128, 180, 212, 249, 303, 404
FatI CATG 3 cut(s) 38, 126, 210
Fnu4HI GCNGC 1 cut(s) 21
FokI GGATG 3 cut(s) 11, 77, 230
Fsp4HI GCNGC 1 cut(s) 21
FspBI CTAG 1 cut(s) 54
GluI GCNGC 1 cut(s) 21
GsaI CCCAGC 1 cut(s) 443
HaeIII GGCC 1 cut(s) 483
HgaI GACGC 1 cut(s) 164
Hin1II CATG 3 cut(s) 42, 130, 214
HinfI GANTC 4 cut(s) 80, 239, 357, 363
HphI GGTGA 2 cut(s) 248, 347
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 6 cut(s) 205, 298, 328, 362, 449, 457
Hpy188III TCNNGA 3 cut(s) 104, 211, 267
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 1 cut(s) 335
HpyAV CCTTC 2 cut(s) 290, 308
HpyCH4III ACNGT 3 cut(s) 77, 335, 352
HpyCH4V TGCA 4 cut(s) 33, 71, 182, 275
HpyF10VI GCNNNNNNNGC 1 cut(s) 403
Hsp92II CATG 3 cut(s) 42, 130, 214
Kzo9I GATC 3 cut(s) 57, 426, 452
LguI GCTCTTC 1 cut(s) 471
LpnPI CCDG 5 cut(s) 18, 147, 252, 446, 453
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 1 cut(s) 208
MaeI CTAG 1 cut(s) 54
MalI GATC 3 cut(s) 59, 428, 454
MboI GATC 3 cut(s) 57, 426, 452
MboII GAAGA 2 cut(s) 458, 462
MluCI AATT 2 cut(s) 118, 422
MlyI GAGTC 1 cut(s) 372
MmeI TCCRAC 1 cut(s) 334
MnlI CCTC 2 cut(s) 141, 223
MseI TTAA 2 cut(s) 90, 414
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 20
Mva1269I GAATGC 1 cut(s) 443
MwoI GCNNNNNNNGC 1 cut(s) 403
NcoI CCATGG 1 cut(s) 38
NdeII GATC 3 cut(s) 57, 426, 452
NlaIII CATG 3 cut(s) 42, 130, 214
NspI RCATGY 1 cut(s) 130
PagI TCATGA 1 cut(s) 210
PciSI GCTCTTC 1 cut(s) 471
PctI GAATGC 1 cut(s) 443
PfeI GAWTC 3 cut(s) 80, 239, 357
PkrI GCNGC 1 cut(s) 22
PleI GAGTC 1 cut(s) 371
PpsI GAGTC 1 cut(s) 371
PspFI CCCAGC 1 cut(s) 439
PspPI GGNCC 1 cut(s) 482
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 3 cut(s) 44, 227, 292
RsaNI GTAC 3 cut(s) 43, 226, 291
RseI CAYNNNNRTG 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 471
SaqAI TTAA 2 cut(s) 90, 414
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 3 cut(s) 57, 426, 452
Sau96I GGNCC 1 cut(s) 482
SchI GAGTC 1 cut(s) 372
SetI ASST 3 cut(s) 17, 22, 399
SfaNI GCATC 1 cut(s) 208
SmiMI CAYNNNNRTG 1 cut(s) 131
Sse9I AATT 2 cut(s) 118, 422
SspMI CTAG 1 cut(s) 54
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 3 cut(s) 77, 335, 352
TasI AATT 2 cut(s) 118, 422
TfiI GAWTC 3 cut(s) 80, 239, 357
Tru1I TTAA 2 cut(s) 90, 414
Tru9I TTAA 2 cut(s) 90, 414
TscAI CASTG 3 cut(s) 12, 82, 189
TseI GCWGC 1 cut(s) 20
TspGWI ACGGA 2 cut(s) 77, 167
TspRI CASTG 3 cut(s) 12, 82, 189
XceI RCATGY 1 cut(s) 130
XspI CTAG 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.