Rh5BG081100

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0022639
Physical Location & Seq
Reverse (-)
6909457 .. 6910774
1318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG081100.1

Sequence Viewer

Length: 612 bp
ATGAGAGGTAATATTCCTGATGATATAGGCAACTTGAGCAGCTTGACAATCTTAAACATGCATGACAACAAATTAAGTGGATCAATTCCAACCTCAATAGGAACACTGAAAAATCTCCAAGTTTTGAGTTTGGACGGTAACAGCTGTCAAGGACACATCGCGGATGAACTTTGTGAACTACAAAACCTTTTCGTATTAAGCTTGCATGGTAACCAGCTCTCTGGCTCTATACCTTCTTGTTTGGGTCATCTAGCCACCGCACTAAGAGCTCTAGATTTAGGGTCCAATTTGTTGAATTCAACAATACCAACTAACTTGTGGGGACTTTCAGATCTCTTGTATGTATACTTGTCATCGAATAATCTAAGTGGATCTCTCTCGGAAGATGTTGGTAATCTTAAAGTTATTATAGAGATAGATTTATCAAACAACCACTTATCCGGTAGCATACCGAGCAGCATTGGGAATCTACAAAGCATGGTCAAGCTCTCCTTGGAGAATAATAAGTTCGAAGGCCTTATTCCTAATTCACTTGGCAAGTCGCTCAGCTTGGAAATTTTAGATTTATCCATAAACAATCTGTCTGGATTGATTCCAAAGTCATTTGAATAG

Protein Analysis

203

Amino Acids

21.56

Weight (kDa)

4.82

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 7 - 71 6.1e-07 Leucine-rich repeat region
LRR_14 PF23598 87 - 190 1.1e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022639)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0115751
rosa_roxburghii Rroxscaffold_1G00065270
rosa_rugosa Rorug04G0454100
rosa_samantha Rh5BG081100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 345
AccII CGCG 1 cut(s) 161
AciI CCGC 2 cut(s) 161, 258
AclWI GGATC 2 cut(s) 88, 379
AcsI RAATTY 2 cut(s) 295, 555
AgsI TTSAA 3 cut(s) 295, 300, 608
AluBI AGCT 7 cut(s) 42, 144, 201, 217, 269, 487, 549
AluI AGCT 7 cut(s) 42, 144, 201, 217, 269, 487, 549
Alw21I GWGCWC 1 cut(s) 271
AlwI GGATC 2 cut(s) 88, 379
AoxI GGCC 1 cut(s) 514
ApeKI GCWGC 2 cut(s) 39, 456
ApoI RAATTY 2 cut(s) 295, 555
AspS9I GGNCC 1 cut(s) 282
AsuII TTCGAA 1 cut(s) 510
AvaII GGWCC 1 cut(s) 282
BanII GRGCYC 1 cut(s) 271
Bbv12I GWGCWC 1 cut(s) 271
BbvI GCAGC 2 cut(s) 51, 468
BfaI CTAG 2 cut(s) 251, 272
BglII AGATCT 1 cut(s) 331
BisI GCNGC 2 cut(s) 40, 457
BlpI GCTNAGC 1 cut(s) 545
BlsI GCNGC 2 cut(s) 41, 458
Bme18I GGWCC 1 cut(s) 282
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 1 cut(s) 283
Bpu1102I GCTNAGC 1 cut(s) 545
Bpu14I TTCGAA 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 55
BsaJI CCNNGG 1 cut(s) 492
BsaWI WCCGGW 1 cut(s) 440
BseDI CCNNGG 1 cut(s) 492
BseGI GGATG 1 cut(s) 169
BseMII CTCAG 1 cut(s) 559
BseXI GCAGC 2 cut(s) 51, 468
Bsh1236I CGCG 1 cut(s) 161
BshFI GGCC 1 cut(s) 516
BsiHKAI GWGCWC 1 cut(s) 271
BsiSI CCGG 1 cut(s) 441
BslFI GGGAC 1 cut(s) 336
BsmFI GGGAC 1 cut(s) 336
BsnI GGCC 1 cut(s) 516
Bsp119I TTCGAA 1 cut(s) 510
Bsp1286I GDGCHC 1 cut(s) 271
Bsp143I GATC 3 cut(s) 80, 331, 371
Bsp1720I GCTNAGC 1 cut(s) 545
BspACI CCGC 2 cut(s) 161, 258
BspANI GGCC 1 cut(s) 516
BspCNI CTCAG 1 cut(s) 558
BspFNI CGCG 1 cut(s) 161
BspLI GGNNCC 1 cut(s) 283
BspPI GGATC 2 cut(s) 88, 379
BspT104I TTCGAA 1 cut(s) 510
BssECI CCNNGG 1 cut(s) 492
BssMI GATC 3 cut(s) 80, 331, 371
BssNAI GTATAC 1 cut(s) 346
BssT1I CCWWGG 1 cut(s) 492
Bst1107I GTATAC 1 cut(s) 346
Bst4CI ACNGT 1 cut(s) 137
BstBI TTCGAA 1 cut(s) 510
BstC8I GCNNGC 1 cut(s) 203
BstDEI CTNAG 3 cut(s) 263, 365, 545
BstEII GGTNACC 1 cut(s) 209
BstF5I GGATG 1 cut(s) 169
BstFNI CGCG 1 cut(s) 161
BstKTI GATC 3 cut(s) 83, 334, 374
BstMBI GATC 3 cut(s) 80, 331, 371
BstMWI GCNNNNNNNGC 3 cut(s) 36, 266, 453
BstNSI RCATGY 1 cut(s) 61
BstPI GGTNACC 1 cut(s) 209
BstUI CGCG 1 cut(s) 161
BstV1I GCAGC 2 cut(s) 51, 468
BstX2I RGATCY 2 cut(s) 331, 371
BstXI CCANNNNNNTGG 1 cut(s) 221
BstYI RGATCY 2 cut(s) 331, 371
BstZ17I GTATAC 1 cut(s) 346
BsuRI GGCC 1 cut(s) 516
BtgZI GCGATG 1 cut(s) 142
BtsCI GGATG 1 cut(s) 169
BtsIMutI CAGTG 1 cut(s) 104
Cac8I GCNNGC 1 cut(s) 203
Cfr13I GGNCC 1 cut(s) 282
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviAII CATG 4 cut(s) 58, 62, 206, 478
DdeI CTNAG 3 cut(s) 263, 365, 545
DpnI GATC 3 cut(s) 82, 333, 373
DpnII GATC 3 cut(s) 80, 331, 371
Ecl136II GAGCTC 1 cut(s) 269
Eco130I CCWWGG 1 cut(s) 492
Eco147I AGGCCT 1 cut(s) 516
Eco24I GRGCYC 1 cut(s) 271
Eco47I GGWCC 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 269
Eco91I GGTNACC 1 cut(s) 209
EcoICRI GAGCTC 1 cut(s) 269
EcoO65I GGTNACC 1 cut(s) 209
EcoRI GAATTC 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 492
EcoT22I ATGCAT 1 cut(s) 63
EcoT38I GRGCYC 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 4 cut(s) 61, 65, 209, 481
FalI AAGNNNNNCTT 2 cut(s) 476, 508
FaqI GGGAC 1 cut(s) 336
FatI CATG 4 cut(s) 57, 61, 205, 477
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 2 cut(s) 40, 457
FokI GGATG 1 cut(s) 176
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 2 cut(s) 40, 457
FspBI CTAG 2 cut(s) 251, 272
GluI GCNGC 2 cut(s) 40, 457
HaeIII GGCC 1 cut(s) 516
HapII CCGG 1 cut(s) 441
Hin1II CATG 4 cut(s) 61, 65, 209, 481
HindIII AAGCTT 1 cut(s) 199
HinfI GANTC 2 cut(s) 466, 592
HpaII CCGG 1 cut(s) 441
Hpy166II GTNNAC 2 cut(s) 176, 346
Hpy188I TCNGA 2 cut(s) 331, 382
Hpy188III TCNNGA 3 cut(s) 17, 272, 585
Hpy8I GTNNAC 2 cut(s) 176, 346
HpyAV CCTTC 2 cut(s) 243, 506
HpyCH4III ACNGT 1 cut(s) 137
HpyCH4V TGCA 2 cut(s) 61, 205
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 266, 453
HpyF3I CTNAG 3 cut(s) 263, 365, 545
Hsp92II CATG 4 cut(s) 61, 65, 209, 481
Kzo9I GATC 3 cut(s) 80, 331, 371
LpnPI CCDG 5 cut(s) 30, 207, 227, 454, 570
Lsp1109I GCAGC 2 cut(s) 51, 468
MaeI CTAG 2 cut(s) 251, 272
MaeIII GTNAC 2 cut(s) 137, 209
MalI GATC 3 cut(s) 82, 333, 373
MboI GATC 3 cut(s) 80, 331, 371
MboII GAAGA 1 cut(s) 395
MflI RGATCY 2 cut(s) 331, 371
MhlI GDGCHC 1 cut(s) 271
MluCI AATT 6 cut(s) 71, 84, 286, 295, 526, 555
MmeI TCCRAC 1 cut(s) 113
MnlI CCTC 1 cut(s) 103
Mph1103I ATGCAT 1 cut(s) 63
MseI TTAA 4 cut(s) 53, 74, 197, 399
MspA1I CMGCKG 1 cut(s) 144
MspI CCGG 1 cut(s) 441
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 3 cut(s) 36, 266, 453
NdeII GATC 3 cut(s) 80, 331, 371
NlaIII CATG 4 cut(s) 61, 65, 209, 481
NlaIV GGNNCC 1 cut(s) 283
NsiI ATGCAT 1 cut(s) 63
NspI RCATGY 1 cut(s) 61
NspV TTCGAA 1 cut(s) 510
PceI AGGCCT 1 cut(s) 516
PfeI GAWTC 2 cut(s) 466, 592
PkrI GCNGC 2 cut(s) 41, 458
Psp124BI GAGCTC 1 cut(s) 271
PspEI GGTNACC 1 cut(s) 209
PspN4I GGNNCC 1 cut(s) 283
PspPI GGNCC 1 cut(s) 282
PsuI RGATCY 2 cut(s) 331, 371
PvuII CAGCTG 1 cut(s) 144
SacI GAGCTC 1 cut(s) 271
SaqAI TTAA 4 cut(s) 53, 74, 197, 399
SatI GCNGC 2 cut(s) 40, 457
Sau3AI GATC 3 cut(s) 80, 331, 371
Sau96I GGNCC 1 cut(s) 282
SduI GDGCHC 1 cut(s) 271
SfuI TTCGAA 1 cut(s) 510
SinI GGWCC 1 cut(s) 282
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
Sse9I AATT 6 cut(s) 71, 84, 286, 295, 526, 555
SseBI AGGCCT 1 cut(s) 516
SsiI CCGC 2 cut(s) 161, 258
SspI AATATT 1 cut(s) 13
SspMI CTAG 2 cut(s) 251, 272
SstI GAGCTC 1 cut(s) 271
StuI AGGCCT 1 cut(s) 516
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 1 cut(s) 137
TaqI TCGA 2 cut(s) 356, 510
TasI AATT 6 cut(s) 71, 84, 286, 295, 526, 555
TfiI GAWTC 2 cut(s) 466, 592
Tru1I TTAA 4 cut(s) 53, 74, 197, 399
Tru9I TTAA 4 cut(s) 53, 74, 197, 399
TscAI CASTG 1 cut(s) 111
TseI GCWGC 2 cut(s) 39, 456
TspDTI ATGAA 1 cut(s) 180
TspRI CASTG 1 cut(s) 111
VpaK11BI GGWCC 1 cut(s) 282
XapI RAATTY 2 cut(s) 295, 555
XbaI TCTAGA 1 cut(s) 271
XceI RCATGY 1 cut(s) 61
XcmI CCANNNNNNNNNTGG 1 cut(s) 315
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 2 cut(s) 251, 272
Zsp2I ATGCAT 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.