Rh5BG098300

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0020376
Physical Location & Seq
Forward (+)
8956044 .. 8958995
2952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG098300.1

Sequence Viewer

Length: 555 bp
ATGAAGCAAACTAGCTCCCTACCATATATTCCTAACCAGCTGCCTTGGTGCAGAGGTTTTAACTGGTGGCTTAGAATGTCGATTTATACAGTCTTTGTCCTCGCTGGCCAGGCAATTGCAACACTATTGGGAAGACAGTACCATGACAAAGGTGGTAAAAGCAACTGGCTTGCTACACTAGTACAACTTTGTGGCTTTCCTATCTTCCTTCCCTATTATTGCATTCCAGCATTGAACAAAAACTGTCCAACCACAAACAGTACTATAGCAATCGAGTCAAAGCCACCGTCTATATTAGTCCTTGCATCAATTTATGCCTCTCTTGGCTCCCTTATAGCAGCAGATCGACTACTACTTGTATTCAGTAGGACTATCTTACCTTCCTGTATCTACTTTCTCCCTCATTTCTGCATCCCAACTCACCTTCAATGCACCTTTCTCCTTCTTCCTCAACTCGCAGAAGTTAACTTTTTTAATTGTCAACTCTCTTTTTATACTGACCATCTCATCCAGCCTCCTAGTGCTTCAAGCAGACCACTCTGCAGACTCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

184

Amino Acids

20.73

Weight (kDa)

8.91

Isoelectric Point (pI)

46.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 25 - 133 4.4e-19 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020376)

Species Orthologous Gene IDs
malus_domestica MD02G1225500.v1.1
rosa_chinensis RchiOBHm_Chr7g0208091
rosa_rugosa Rorug05G0007800
rosa_samantha Rh5BG098300 Rh5CG110500 Rh5DG097600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 106
AfaI GTAC 3 cut(s) 140, 183, 262
AgsI TTSAA 3 cut(s) 235, 428, 528
AhlI ACTAGT 1 cut(s) 178
AjnI CCWGG 1 cut(s) 108
AluBI AGCT 2 cut(s) 15, 40
AluI AGCT 2 cut(s) 15, 40
Ama87I CYCGRG 1 cut(s) 548
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 2 cut(s) 40, 338
ArsI GACNNNNNNTTYG 2 cut(s) 272, 304
AsuHPI GGTGA 1 cut(s) 413
AvaI CYCGRG 1 cut(s) 548
BalI TGGCCA 1 cut(s) 108
BbsI GAAGAC 1 cut(s) 139
BbvI GCAGC 2 cut(s) 27, 350
BccI CCATC 1 cut(s) 510
BciT130I CCWGG 1 cut(s) 110
BcuI ACTAGT 1 cut(s) 178
BfaI CTAG 3 cut(s) 12, 179, 519
BfmI CTRYAG 2 cut(s) 264, 541
BisI GCNGC 2 cut(s) 41, 339
BlsI GCNGC 2 cut(s) 42, 340
BmcAI AGTACT 1 cut(s) 262
Bme1390I CCNGG 1 cut(s) 110
BmeT110I CYCGRG 1 cut(s) 548
BmiI GGNNCC 1 cut(s) 328
BmrFI CCNGG 1 cut(s) 110
BmsI GCATC 2 cut(s) 314, 420
BpiI GAAGAC 1 cut(s) 139
BsaJI CCNNGG 1 cut(s) 44
Bse1I ACTGG 2 cut(s) 68, 170
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 1 cut(s) 44
BseGI GGATG 2 cut(s) 411, 507
BseNI ACTGG 2 cut(s) 68, 170
BseXI GCAGC 2 cut(s) 27, 350
BsgI GTGCAG 1 cut(s) 70
BshFI GGCC 1 cut(s) 108
BsiHKCI CYCGRG 1 cut(s) 548
BsmI GAATGC 1 cut(s) 222
BsnI GGCC 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 548
Bsp143I GATC 1 cut(s) 343
BspANI GGCC 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 328
BspMAI CTGCAG 1 cut(s) 545
BsrI ACTGG 2 cut(s) 68, 170
BssECI CCNNGG 1 cut(s) 44
BssMI GATC 1 cut(s) 343
BssT1I CCWWGG 1 cut(s) 44
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 5 cut(s) 91, 138, 245, 260, 288
BstC8I GCNNGC 2 cut(s) 106, 171
BstDEI CTNAG 1 cut(s) 71
BstF5I GGATG 2 cut(s) 411, 507
BstKTI GATC 1 cut(s) 346
BstMBI GATC 1 cut(s) 343
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstNI CCWGG 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 108
BstSFI CTRYAG 2 cut(s) 264, 541
BstV1I GCAGC 2 cut(s) 27, 350
BstV2I GAAGAC 1 cut(s) 139
BsuRI GGCC 1 cut(s) 108
BtsCI GGATG 2 cut(s) 411, 507
Cac8I GCNNGC 2 cut(s) 106, 171
Csp6I GTAC 3 cut(s) 139, 182, 261
CviAII CATG 1 cut(s) 143
CviJI RGCY 9 cut(s) 15, 40, 70, 108, 169, 195, 283, 327, 514
CviKI_1 RGCY 9 cut(s) 15, 40, 70, 108, 169, 195, 283, 327, 514
CviQI GTAC 3 cut(s) 139, 182, 261
DdeI CTNAG 1 cut(s) 71
DpnI GATC 1 cut(s) 345
DpnII GATC 1 cut(s) 343
EaeI YGGCCR 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 44
Eco88I CYCGRG 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 44
FaeI CATG 1 cut(s) 146
FaiI YATR 9 cut(s) 25, 27, 87, 144, 266, 293, 315, 335, 495
FatI CATG 1 cut(s) 142
Fnu4HI GCNGC 2 cut(s) 41, 339
FokI GGATG 2 cut(s) 398, 494
Fsp4HI GCNGC 2 cut(s) 41, 339
FspBI CTAG 3 cut(s) 12, 179, 519
GluI GCNGC 2 cut(s) 41, 339
HaeIII GGCC 1 cut(s) 108
Hin1II CATG 1 cut(s) 146
HincII GTYRAC 2 cut(s) 466, 482
HindII GTYRAC 2 cut(s) 466, 482
HinfI GANTC 2 cut(s) 275, 546
HpaI GTTAAC 1 cut(s) 466
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 2 cut(s) 466, 482
Hpy8I GTNNAC 2 cut(s) 466, 482
HpyAV CCTTC 4 cut(s) 218, 390, 434, 452
HpyCH4III ACNGT 5 cut(s) 91, 138, 245, 260, 288
HpyCH4V TGCA 7 cut(s) 51, 119, 222, 305, 411, 432, 543
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 1 cut(s) 71
Hsp92II CATG 1 cut(s) 146
KspAI GTTAAC 1 cut(s) 466
Kzo9I GATC 1 cut(s) 343
LmnI GCTCC 2 cut(s) 20, 332
LpnPI CCDG 9 cut(s) 49, 50, 90, 95, 122, 151, 240, 397, 524
Lsp1109I GCAGC 2 cut(s) 27, 350
LweI GCATC 2 cut(s) 314, 420
MaeI CTAG 3 cut(s) 12, 179, 519
MalI GATC 1 cut(s) 345
MboI GATC 1 cut(s) 343
MboII GAAGA 3 cut(s) 144, 196, 437
MfeI CAATTG 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 108
MluCI AATT 3 cut(s) 114, 309, 475
MluNI TGGCCA 1 cut(s) 108
MlyI GAGTC 2 cut(s) 284, 540
MmeI TCCRAC 1 cut(s) 272
MnlI CCTC 6 cut(s) 47, 110, 328, 411, 459, 525
Mox20I TGGCCA 1 cut(s) 108
MscI TGGCCA 1 cut(s) 108
MseI TTAA 3 cut(s) 60, 465, 474
Msp20I TGGCCA 1 cut(s) 108
MspA1I CMGCKG 1 cut(s) 40
MspR9I CCNGG 1 cut(s) 110
MunI CAATTG 1 cut(s) 114
Mva1269I GAATGC 1 cut(s) 222
MvaI CCWGG 1 cut(s) 110
MwoI GCNNNNNNNGC 1 cut(s) 110
NdeII GATC 1 cut(s) 343
NlaIII CATG 1 cut(s) 146
NlaIV GGNNCC 1 cut(s) 328
PaeR7I CTCGAG 1 cut(s) 548
PctI GAATGC 1 cut(s) 222
PkrI GCNGC 2 cut(s) 42, 340
PleI GAGTC 2 cut(s) 283, 540
PpsI GAGTC 2 cut(s) 283, 540
Psp6I CCWGG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 108
PspN4I GGNNCC 1 cut(s) 328
PspXI VCTCGAGB 1 cut(s) 548
PstI CTGCAG 1 cut(s) 545
PvuII CAGCTG 1 cut(s) 40
RsaI GTAC 3 cut(s) 140, 183, 262
RsaNI GTAC 3 cut(s) 139, 182, 261
SaqAI TTAA 3 cut(s) 60, 465, 474
SatI GCNGC 2 cut(s) 41, 339
Sau3AI GATC 1 cut(s) 343
ScaI AGTACT 1 cut(s) 262
SchI GAGTC 2 cut(s) 284, 540
ScrFI CCNGG 1 cut(s) 110
SetI ASST 7 cut(s) 17, 42, 58, 154, 382, 426, 437
SfaNI GCATC 2 cut(s) 314, 420
SfcI CTRYAG 2 cut(s) 264, 541
Sfr274I CTCGAG 1 cut(s) 548
SlaI CTCGAG 1 cut(s) 548
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
SpeI ACTAGT 1 cut(s) 178
Sse9I AATT 3 cut(s) 114, 309, 475
SspMI CTAG 3 cut(s) 12, 179, 519
StyD4I CCNGG 1 cut(s) 108
StyI CCWWGG 1 cut(s) 44
TaaI ACNGT 5 cut(s) 91, 138, 245, 260, 288
TaqI TCGA 4 cut(s) 80, 273, 346, 549
TasI AATT 3 cut(s) 114, 309, 475
TatI WGTACW 2 cut(s) 181, 260
Tru1I TTAA 3 cut(s) 60, 465, 474
Tru9I TTAA 3 cut(s) 60, 465, 474
TseI GCWGC 2 cut(s) 40, 338
TspDTI ATGAA 1 cut(s) 17
XcmI CCANNNNNNNNNTGG 1 cut(s) 149
XhoI CTCGAG 1 cut(s) 548
XspI CTAG 3 cut(s) 12, 179, 519
ZrmI AGTACT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.