Rh5BG404900

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0019935
Physical Location & Seq
Reverse (-)
65718630 .. 65719598
969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG404900.1

Sequence Viewer

Length: 969 bp
ATGGAGTTCTCAGATGTGGCATTTCGAGTTTTGTCCGACGATTATGTTAAAGGTGACAGTGGTACTGGAGTTGTTCACTGTGCTCCTGCATTTGGTGAGGATGACTACCGCGTTTGCCTTGCAAATGAAATAATTAAAAAGGGAGAGGGCTTGATTGTACCAGTTGATGAGAATGGGTGCTTTACAGAAAGAGTTGCTGACTTCAGCAACCTCTATGTGAAGGAGGCAGATAAGGCTATTATTGAAGCAGTGAGGCTAAAGGGAAGGCCTGTTAAGTCTGGAACCATAACTCATTCGTCTCCCTTTTGCTGGAGAACAAACACTCCACTTATCTACAGAGCTGTTCCAAGCTGGTTTATCCGGGTGGAGCAGTTGAAAGAAAAGTTATTGGAAAACAACAAGCAGACATATTGGGTTCCTGATTTTGTGAAGGAAAAGCGATTCCACAATTGGCTAGAAAATGCAAGAGATTGGGCGGTTAGCAGAAGTAGATTTTGGGGTACTCCTCTTCCTGTGTGGGTTAGTGAAGATGGTGAGGAAGTACTGGTCATGGATTCCATTGAGAAGCTTGAAAAGCTTTCTGGTGTTAAGGTTTCTGATCTGCACCGCCACTACATTGATCATATCACTATTCCATCGGCGCGTGGCCCTGAATTTGGTTATCTTCGACGTGTAGACGATGTGTTTGATTGTTGGTTTGAGAGTGGATCCATGCCTTATGCTTATATCCACTATCCTTTCGAGAATGTGGAGCTTTTTGAGAAAAACTTGCCTGGGAACTTTGTGGCCGAAGGGCTTGATCAGACTCGTGGATGGTTCTACACTCTTATGGTGCTATCTACTGCATTATTTGGGAAACCTGCTGCTTTTAAGAACCTCATTTGCAATGGACTTGTTCTTGCTGATGATGGGAAAAAGTTTTTAAAACTTTCCTCCAGTTATGGAGGTTCTCGATCAATATGGGGCTGA

Protein Analysis

322

Amino Acids

36.72

Weight (kDa)

5.74

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 68 - 309 3.7e-64 tRNA synthetases class I (I, L, M and V)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019935)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0060201
rosa_multiflora Rmu_sc0003366.1_g000008
rosa_samantha Rh5AG392400 Rh5AG392500 Rh5BG404900 Rh5CG429000 Rh5DG420600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 868
AccI GTMKAC 1 cut(s) 675
AccII CGCG 2 cut(s) 111, 643
AciI CCGC 3 cut(s) 109, 476, 607
AclWI GGATC 2 cut(s) 702, 715
AcoI YGGCCR 1 cut(s) 786
AcsI RAATTY 1 cut(s) 653
AcuI CTGAAG 1 cut(s) 187
AfaI GTAC 4 cut(s) 64, 159, 502, 543
AfiI CCNNNNNNNGG 3 cut(s) 92, 309, 656
AflIII ACRYGT 1 cut(s) 670
AgsI TTSAA 3 cut(s) 245, 376, 572
AjiI CACGTC 1 cut(s) 671
AjnI CCWGG 1 cut(s) 772
AjuI GAANNNNNNNTTGG 2 cut(s) 478, 510
AloI GAACNNNNNNTCC 2 cut(s) 307, 339
AluBI AGCT 5 cut(s) 341, 351, 568, 577, 754
AluI AGCT 5 cut(s) 341, 351, 568, 577, 754
Alw21I GWGCWC 1 cut(s) 85
Alw26I GTCTC 1 cut(s) 303
AlwI GGATC 2 cut(s) 702, 715
AoxI GGCC 3 cut(s) 266, 646, 786
ApeKI GCWGC 1 cut(s) 863
ApoI RAATTY 1 cut(s) 653
ArsI GACNNNNNNTTYG 2 cut(s) 668, 700
AspLEI GCGC 1 cut(s) 643
AspS9I GGNCC 1 cut(s) 647
AsuC2I CCSGG 1 cut(s) 362
AsuHPI GGTGA 3 cut(s) 65, 107, 545
BaeI ACNNNNGTAYC 2 cut(s) 54, 87
BamHI GGATCC 1 cut(s) 707
BauI CACGAG 1 cut(s) 807
Bbv12I GWGCWC 1 cut(s) 85
BbvI GCAGC 1 cut(s) 850
BccI CCATC 4 cut(s) 524, 643, 807, 902
BciT130I CCWGG 1 cut(s) 774
BclI TGATCA 2 cut(s) 619, 799
BcnI CCSGG 1 cut(s) 362
BcoDI GTCTC 1 cut(s) 303
BfaI CTAG 1 cut(s) 455
BfmI CTRYAG 1 cut(s) 334
BfuAI ACCTGC 1 cut(s) 868
BisI GCNGC 1 cut(s) 864
BlsI GCNGC 1 cut(s) 865
BmcAI AGTACT 1 cut(s) 543
Bme1390I CCNGG 2 cut(s) 362, 774
BmgBI CACGTC 1 cut(s) 671
BmgT120I GGNCC 1 cut(s) 647
BmiI GGNNCC 3 cut(s) 283, 417, 709
BmrFI CCNGG 2 cut(s) 362, 774
BpmI CTGGAG 3 cut(s) 87, 331, 919
BpuMI CCSGG 1 cut(s) 362
BsaJI CCNNGG 1 cut(s) 773
BsaXI ACNNNNNCTCC 2 cut(s) 307, 337
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 309, 656
Bse1I ACTGG 4 cut(s) 70, 161, 549, 936
Bse3DI GCAATG 1 cut(s) 892
BseBI CCWGG 1 cut(s) 774
BseDI CCNNGG 1 cut(s) 773
BseGI GGATG 2 cut(s) 106, 818
BseLI CCNNNNNNNGG 3 cut(s) 92, 309, 656
BseMI GCAATG 1 cut(s) 892
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 4 cut(s) 70, 161, 549, 936
BseRI GAGGAG 1 cut(s) 495
BseXI GCAGC 1 cut(s) 850
BsgI GTGCAG 1 cut(s) 587
Bsh1236I CGCG 2 cut(s) 111, 643
BshFI GGCC 3 cut(s) 268, 648, 788
BsiHKAI GWGCWC 1 cut(s) 85
BsiSI CCGG 1 cut(s) 361
BslI CCNNNNNNNGG 3 cut(s) 92, 309, 656
BsmAI GTCTC 1 cut(s) 303
BsmBI CGTCTC 1 cut(s) 303
BsnI GGCC 3 cut(s) 268, 648, 788
Bsp1286I GDGCHC 1 cut(s) 85
Bsp143I GATC 5 cut(s) 598, 619, 707, 799, 953
BspACI CCGC 3 cut(s) 109, 476, 607
BspANI GGCC 3 cut(s) 268, 648, 788
BspCNI CTCAG 1 cut(s) 23
BspFNI CGCG 2 cut(s) 111, 643
BspLI GGNNCC 3 cut(s) 283, 417, 709
BspMI ACCTGC 1 cut(s) 868
BspPI GGATC 2 cut(s) 702, 715
BsrDI GCAATG 1 cut(s) 892
BsrI ACTGG 4 cut(s) 70, 161, 549, 936
BssECI CCNNGG 1 cut(s) 773
BssMI GATC 5 cut(s) 598, 619, 707, 799, 953
BssSI CACGAG 1 cut(s) 807
Bst2BI CACGAG 1 cut(s) 807
Bst2UI CCWGG 1 cut(s) 774
Bst4CI ACNGT 2 cut(s) 59, 80
Bst6I CTCTTC 1 cut(s) 513
BstDEI CTNAG 1 cut(s) 10
BstF5I GGATG 2 cut(s) 106, 818
BstFNI CGCG 2 cut(s) 111, 643
BstHHI GCGC 1 cut(s) 643
BstKTI GATC 5 cut(s) 601, 622, 710, 802, 956
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 5 cut(s) 598, 619, 707, 799, 953
BstMWI GCNNNNNNNGC 2 cut(s) 233, 574
BstNI CCWGG 1 cut(s) 774
BstSCI CCNGG 2 cut(s) 360, 772
BstSFI CTRYAG 1 cut(s) 334
BstUI CGCG 2 cut(s) 111, 643
BstV1I GCAGC 1 cut(s) 850
BstX2I RGATCY 1 cut(s) 707
BstYI RGATCY 1 cut(s) 707
BsuRI GGCC 3 cut(s) 268, 648, 788
BtrI CACGTC 1 cut(s) 671
BtsCI GGATG 2 cut(s) 106, 818
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 3 cut(s) 64, 76, 255
BveI ACCTGC 1 cut(s) 868
CfoI GCGC 1 cut(s) 643
Cfr13I GGNCC 1 cut(s) 647
Csp6I GTAC 4 cut(s) 63, 158, 501, 542
CviAII CATG 2 cut(s) 550, 712
CviQI GTAC 4 cut(s) 63, 158, 501, 542
DdeI CTNAG 1 cut(s) 10
DpnI GATC 5 cut(s) 600, 621, 709, 801, 955
DpnII GATC 5 cut(s) 598, 619, 707, 799, 953
DraI TTTAAA 1 cut(s) 924
EaeI YGGCCR 1 cut(s) 786
Eam1104I CTCTTC 1 cut(s) 513
EarI CTCTTC 1 cut(s) 513
Eco147I AGGCCT 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 772
Esp3I CGTCTC 1 cut(s) 303
FaeI CATG 2 cut(s) 553, 715
FatI CATG 2 cut(s) 549, 711
FbaI TGATCA 2 cut(s) 619, 799
FblI GTMKAC 1 cut(s) 675
Fnu4HI GCNGC 1 cut(s) 864
FokI GGATG 2 cut(s) 113, 825
Fsp4HI GCNGC 1 cut(s) 864
FspBI CTAG 1 cut(s) 455
GlaI GCGC 1 cut(s) 642
GluI GCNGC 1 cut(s) 864
GsuI CTGGAG 3 cut(s) 87, 331, 919
HaeIII GGCC 3 cut(s) 268, 648, 788
HapII CCGG 1 cut(s) 361
HhaI GCGC 1 cut(s) 643
Hin1II CATG 2 cut(s) 553, 715
Hin6I GCGC 1 cut(s) 641
HinP1I GCGC 1 cut(s) 641
HindIII AAGCTT 2 cut(s) 566, 575
HinfI GANTC 3 cut(s) 441, 554, 805
HpaII CCGG 1 cut(s) 361
HphI GGTGA 3 cut(s) 65, 107, 545
Hpy166II GTNNAC 2 cut(s) 76, 676
Hpy188I TCNGA 4 cut(s) 13, 37, 598, 804
Hpy188III TCNNGA 4 cut(s) 279, 419, 742, 951
Hpy8I GTNNAC 2 cut(s) 76, 676
Hpy99I CGWCG 2 cut(s) 41, 672
HpyAV CCTTC 4 cut(s) 214, 258, 424, 785
HpyCH4III ACNGT 2 cut(s) 59, 80
HpyCH4IV ACGT 1 cut(s) 670
HpyCH4V TGCA 6 cut(s) 89, 122, 464, 604, 845, 885
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 574
HpyF3I CTNAG 1 cut(s) 10
HpySE526I ACGT 1 cut(s) 670
Hsp92II CATG 2 cut(s) 553, 715
HspAI GCGC 1 cut(s) 641
Ksp22I TGATCA 2 cut(s) 619, 799
Kzo9I GATC 5 cut(s) 598, 619, 707, 799, 953
LmnI GCTCC 3 cut(s) 88, 367, 751
Lsp1109I GCAGC 1 cut(s) 850
MaeI CTAG 1 cut(s) 455
MaeII ACGT 1 cut(s) 670
MaeIII GTNAC 1 cut(s) 53
MalI GATC 5 cut(s) 600, 621, 709, 801, 955
MboI GATC 5 cut(s) 598, 619, 707, 799, 953
MboII GAAGA 3 cut(s) 500, 539, 656
MfeI CAATTG 1 cut(s) 448
MflI RGATCY 1 cut(s) 707
MhlI GDGCHC 1 cut(s) 85
MluCI AATT 3 cut(s) 132, 448, 653
MlyI GAGTC 1 cut(s) 799
MmeI TCCRAC 1 cut(s) 60
MseI TTAA 6 cut(s) 48, 135, 273, 588, 870, 923
MslI CAYNNNNRTG 1 cut(s) 827
MspI CCGG 1 cut(s) 361
MspR9I CCNGG 2 cut(s) 362, 774
MunI CAATTG 1 cut(s) 448
MvaI CCWGG 1 cut(s) 774
MvnI CGCG 2 cut(s) 111, 643
MwoI GCNNNNNNNGC 2 cut(s) 233, 574
NciI CCSGG 1 cut(s) 362
NdeII GATC 5 cut(s) 598, 619, 707, 799, 953
NlaIII CATG 2 cut(s) 553, 715
NlaIV GGNNCC 3 cut(s) 283, 417, 709
NmuCI GTSAC 1 cut(s) 53
PceI AGGCCT 1 cut(s) 268
PfeI GAWTC 2 cut(s) 441, 554
PkrI GCNGC 1 cut(s) 865
PleI GAGTC 1 cut(s) 799
PpsI GAGTC 1 cut(s) 799
Psp6I CCWGG 1 cut(s) 772
PspGI CCWGG 1 cut(s) 772
PspN4I GGNNCC 3 cut(s) 283, 417, 709
PspPI GGNCC 1 cut(s) 647
PsuI RGATCY 1 cut(s) 707
RsaI GTAC 4 cut(s) 64, 159, 502, 543
RsaNI GTAC 4 cut(s) 63, 158, 501, 542
RseI CAYNNNNRTG 1 cut(s) 827
SaqAI TTAA 6 cut(s) 48, 135, 273, 588, 870, 923
SatI GCNGC 1 cut(s) 864
Sau3AI GATC 5 cut(s) 598, 619, 707, 799, 953
Sau96I GGNCC 1 cut(s) 647
ScaI AGTACT 1 cut(s) 543
SchI GAGTC 1 cut(s) 799
ScrFI CCNGG 2 cut(s) 362, 774
SduI GDGCHC 1 cut(s) 85
SfcI CTRYAG 1 cut(s) 334
SmiMI CAYNNNNRTG 1 cut(s) 827
Sse9I AATT 3 cut(s) 132, 448, 653
SseBI AGGCCT 1 cut(s) 268
SsiI CCGC 3 cut(s) 109, 476, 607
SspMI CTAG 1 cut(s) 455
StuI AGGCCT 1 cut(s) 268
StyD4I CCNGG 2 cut(s) 360, 772
TaaI ACNGT 2 cut(s) 59, 80
TaiI ACGT 1 cut(s) 673
TaqI TCGA 4 cut(s) 25, 667, 741, 952
TasI AATT 3 cut(s) 132, 448, 653
TatI WGTACW 1 cut(s) 541
TfiI GAWTC 2 cut(s) 441, 554
Tru1I TTAA 6 cut(s) 48, 135, 273, 588, 870, 923
Tru9I TTAA 6 cut(s) 48, 135, 273, 588, 870, 923
TscAI CASTG 3 cut(s) 64, 83, 255
TseFI GTSAC 1 cut(s) 53
TseI GCWGC 1 cut(s) 863
Tsp45I GTSAC 1 cut(s) 53
TspDTI ATGAA 1 cut(s) 141
TspRI CASTG 3 cut(s) 64, 83, 255
XapI RAATTY 1 cut(s) 653
XmiI GTMKAC 1 cut(s) 675
XspI CTAG 1 cut(s) 455
ZrmI AGTACT 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.