Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0019935

Physical Location & Seq

Genomic Coordinates Reverse (-)

66944351 .. 66948089

Gene Structure 3739 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG420600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1452 bp

ATGTCACGCGCCGCGCGCCGCTTTGGCTGGGACTGCCATGGCTTGCCTGTTGAGACCGAGATCGATAAGAAGCTCAACATCAAGACCCGACAAGATGTTCTGAACCTGGGAATTGATGCCTACAATGAAGAATGTAGGAGCTTTGTTACTCGATATGTGGAGGAATGGGAGAAGGTGGTTACTCGCTCGGGGCGGTGGATCGATTTCAGGAATGATTATAAGACTATGGACTTGAAGTTCATGGAGAGTGTGTGGTGGGTTTTTGCTCAATTATATGAAAAGGGTCTTGTTTACAAAGACTTCAAGGTCATGCTTTACAGCACTGGTTGCAAGACACCGCTCTCCAATTTTGAGGCCGGTAAAAATCACAAGGATGTGTCTGATCCTGAAATAATGGTAGCTTTCCCAATTGTCGGTGATCTGGATAAGGCAGCGTTTGTGGCTTGGACAACTACTCCATGGACCCTTCCTAGCAATTTAGCCCTGTTTGTCAATCCAAATTTAACCTATGTGAAGGTTTGCAACAGGTGTTCTGGAAGAGTTTATGTTGTTGCTGAATCCCGGGTTTCATCTCTACCTAGTGAGAGACCTAGAGGAGCTGCTTATGACGGTTCAAACATTTATGAAGTGTTGGAGAGATTCTCAGGGACTACACTAGTGGGAAAGAAGGATGAGCCACTTTTTGATTACTTCATGGAGTTCTCAGATGTGGCATTTCGAGTTTTGTCCGACGATTATGTTAAAGGTGACAGTGGTACTGGAGTTGTTCACTGTGCTCCTGCATTTGGTGAGGATGACTACCGCGTTTGCCTTGCAAATGAAGTAATCAAAAAGGGAGAGGGCTTGATTGTACCAGTTGATGAGAATGGGTGCTTTACAGAAAGAGTTGCTGACTTCAGCAACCTCTATGTGAAGGAGGCAGATAAGGCTATTATTGAAGCAGTGAGGCTAAAGGGAAGGCTTGTTAAGTCTGGAACCATAACTCATTCGTATCCCTTTTGCTGGAGAACAAACACTCCACTTATCTACAGAGCTGTTCCAAGCTGGTTTATCCGGGTGGAGCAGTTGAAAGAAAAGTTATTGGAAAACAACAAGCAGACATATTGGGTTCCTGATTTTGTGAAGGAAAAGCGATTCCACAATTGGCTAGAAAATGCAAGAGATTGGGCGGTTAGCAGAAGTAGATTTTGGGGTACTCCTCTTCCTGTGTGGGTTAGTGAGGATGGTGAGGAAGTACTGGTCATGGATTCCATTGAGAAGCTTGAAAAGCTTTCTGGTGTTAAGGTTTCTGATCTGCACCGCCATTACATTGATCATATCACTATTCCATCGGCGCGTGGCACTGAATTTGGTTATCTTCGACGTGTAGACGATGTGTTTGATTGTTGGTTTGAGAGTGGATCCATGCCTTATCCTTATATCCACTATCCTTTCGAGAATGTGGAGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004822 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006428 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0017101 GO:0019538 GO:0019752 GO:0019899 GO:0032991 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0051020 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1

Protein Analysis

483

Amino Acids

55.82

Weight (kDa)

5.66

Isoelectric Point (pI)

37.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	4 - 480	4.9e-90	tRNA synthetases class I (I, L, M and V)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0019935)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr5g0060201
rosa_multiflora	Rmu_sc0003366.1_g000008
rosa_samantha	Rh5AG392400 Rh5AG392500 Rh5BG404900 Rh5CG429000 Rh5DG420600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	219
AasI	GACNNNNNNGTC	1 cut(s)	305
AccBSI	CCGCTC	1 cut(s)	340
AccI	GTMKAC	1 cut(s)	1368
AccII	CGCG	5 cut(s)	9, 14, 16, 804, 1336
AciI	CCGC	7 cut(s)	12, 19, 193, 338, 802, 1169, 1300
AclWI	GGATC	4 cut(s)	206, 377, 1395, 1408
AcsI	RAATTY	2 cut(s)	499, 1346
AcuI	CTGAAG	1 cut(s)	880
AfaI	GTAC	4 cut(s)	757, 852, 1195, 1236
AfiI	CCNNNNNNNGG	3 cut(s)	413, 785, 1002
AflIII	ACRYGT	1 cut(s)	1363
AgsI	TTSAA	6 cut(s)	235, 304, 615, 938, 1069, 1265
AhlI	ACTAGT	1 cut(s)	655
AjiI	CACGTC	1 cut(s)	1364
AjnI	CCWGG	1 cut(s)	105
AjuI	GAANNNNNNNTTGG	2 cut(s)	1171, 1203
AloI	GAACNNNNNNTCC	4 cut(s)	221, 253, 1000, 1032
AluBI	AGCT	9 cut(s)	73, 141, 401, 599, 1034, 1044, 1261, 1270, 1447
AluI	AGCT	9 cut(s)	73, 141, 401, 599, 1034, 1044, 1261, 1270, 1447
Alw21I	GWGCWC	1 cut(s)	778
Alw26I	GTCTC	2 cut(s)	47, 580
AlwI	GGATC	4 cut(s)	206, 377, 1395, 1408
Ama87I	CYCGRG	2 cut(s)	187, 561
AoxI	GGCC	1 cut(s)	354
ApeKI	GCWGC	2 cut(s)	431, 599
ApoI	RAATTY	2 cut(s)	499, 1346
ArsI	GACNNNNNNTTYG	2 cut(s)	1361, 1393
AspLEI	GCGC	4 cut(s)	11, 16, 18, 1336
AspS9I	GGNCC	1 cut(s)	462
AsuC2I	CCSGG	3 cut(s)	562, 563, 1055
AsuHPI	GGTGA	4 cut(s)	428, 758, 800, 1238
AvaI	CYCGRG	2 cut(s)	187, 561
AvaII	GGWCC	1 cut(s)	462
BaeI	ACNNNNGTAYC	2 cut(s)	747, 780
BamHI	GGATCC	1 cut(s)	1400
Bbv12I	GWGCWC	1 cut(s)	778
BbvI	GCAGC	2 cut(s)	443, 586
BccI	CCATC	2 cut(s)	1217, 1336
BciT130I	CCWGG	1 cut(s)	107
BciVI	GTATCC	1 cut(s)	1002
BclI	TGATCA	1 cut(s)	1312
BcnI	CCSGG	3 cut(s)	562, 563, 1055
BcoDI	GTCTC	2 cut(s)	47, 580
BcuI	ACTAGT	1 cut(s)	655
BfaI	CTAG	5 cut(s)	471, 579, 591, 656, 1148
BfmI	CTRYAG	1 cut(s)	1027
BfuI	GTATCC	1 cut(s)	1002
BglI	GCCNNNNNGGC	1 cut(s)	24
BisI	GCNGC	4 cut(s)	12, 19, 432, 600
BlsI	GCNGC	4 cut(s)	13, 20, 433, 601
BmcAI	AGTACT	1 cut(s)	1236
Bme1390I	CCNGG	4 cut(s)	107, 562, 563, 1055
Bme18I	GGWCC	1 cut(s)	462
BmeT110I	CYCGRG	2 cut(s)	187, 561
BmgBI	CACGTC	1 cut(s)	1364
BmgT120I	GGNCC	1 cut(s)	462
BmiI	GGNNCC	4 cut(s)	464, 976, 1110, 1402
BmrFI	CCNGG	4 cut(s)	107, 562, 563, 1055
BmsI	GCATC	1 cut(s)	106
BplI	GAGNNNNNCTC	2 cut(s)	626, 658
BpmI	CTGGAG	2 cut(s)	780, 1024
BpuMI	CCSGG	3 cut(s)	562, 563, 1055
Bsa29I	ATCGAT	2 cut(s)	63, 201
BsaI	GGTCTC	2 cut(s)	47, 580
BsaJI	CCNNGG	4 cut(s)	37, 106, 458, 561
BsaXI	ACNNNNNCTCC	6 cut(s)	236, 266, 439, 469, 1000, 1030
Bsc4I	CCNNNNNNNGG	3 cut(s)	413, 785, 1002
Bse118I	RCCGGY	1 cut(s)	356
Bse1I	ACTGG	4 cut(s)	328, 763, 854, 1242
BseBI	CCWGG	1 cut(s)	107
BseCI	ATCGAT	2 cut(s)	63, 201
BseDI	CCNNGG	4 cut(s)	37, 106, 458, 561
BseGI	GGATG	4 cut(s)	379, 676, 799, 1228
BseLI	CCNNNNNNNGG	3 cut(s)	413, 785, 1002
BseMII	CTCAG	2 cut(s)	657, 717
BseNI	ACTGG	4 cut(s)	328, 763, 854, 1242
BsePI	GCGCGC	1 cut(s)	14
BseRI	GAGGAG	2 cut(s)	609, 1188
BseXI	GCAGC	2 cut(s)	443, 586
BseYI	CCCAGC	1 cut(s)	27
BsgI	GTGCAG	1 cut(s)	1280
Bsh1236I	CGCG	5 cut(s)	9, 14, 16, 804, 1336
BshFI	GGCC	1 cut(s)	356
BshVI	ATCGAT	2 cut(s)	63, 201
BsiHKAI	GWGCWC	1 cut(s)	778
BsiHKCI	CYCGRG	2 cut(s)	187, 561
BsiSI	CCGG	3 cut(s)	357, 562, 1054
BslFI	GGGAC	2 cut(s)	44, 661
BslI	CCNNNNNNNGG	3 cut(s)	413, 785, 1002
BsmAI	GTCTC	2 cut(s)	47, 580
BsmFI	GGGAC	2 cut(s)	44, 661
BsnI	GGCC	1 cut(s)	356
Bso31I	GGTCTC	2 cut(s)	47, 580
BsoBI	CYCGRG	2 cut(s)	187, 561
Bsp1286I	GDGCHC	1 cut(s)	778
Bsp143I	GATC	7 cut(s)	60, 198, 382, 418, 1291, 1312, 1400
Bsp19I	CCATGG	2 cut(s)	37, 458
BspACI	CCGC	7 cut(s)	12, 19, 193, 338, 802, 1169, 1300
BspANI	GGCC	1 cut(s)	356
BspCNI	CTCAG	2 cut(s)	656, 716
BspDI	ATCGAT	2 cut(s)	63, 201
BspFNI	CGCG	5 cut(s)	9, 14, 16, 804, 1336
BspLI	GGNNCC	4 cut(s)	464, 976, 1110, 1402
BspPI	GGATC	4 cut(s)	206, 377, 1395, 1408
BspTNI	GGTCTC	2 cut(s)	47, 580
BsrBI	CCGCTC	1 cut(s)	340
BsrFI	RCCGGY	1 cut(s)	356
BsrI	ACTGG	4 cut(s)	328, 763, 854, 1242
BssAI	RCCGGY	1 cut(s)	356
BssECI	CCNNGG	4 cut(s)	37, 106, 458, 561
BssHII	GCGCGC	1 cut(s)	14
BssMI	GATC	7 cut(s)	60, 198, 382, 418, 1291, 1312, 1400
BssT1I	CCWWGG	2 cut(s)	37, 458
Bst2UI	CCWGG	1 cut(s)	107
Bst4CI	ACNGT	3 cut(s)	611, 752, 773
Bst6I	CTCTTC	2 cut(s)	532, 1206
BstAPI	GCANNNNNTGC	1 cut(s)	327
BstC8I	GCNNGC	2 cut(s)	16, 44
BstDEI	CTNAG	2 cut(s)	643, 703
BstDSI	CCRYGG	2 cut(s)	37, 458
BstF5I	GGATG	4 cut(s)	379, 676, 799, 1228
BstFNI	CGCG	5 cut(s)	9, 14, 16, 804, 1336
BstHHI	GCGC	4 cut(s)	11, 16, 18, 1336
BstKTI	GATC	7 cut(s)	63, 201, 385, 421, 1294, 1315, 1403
BstMAI	GTCTC	2 cut(s)	47, 580
BstMBI	GATC	7 cut(s)	60, 198, 382, 418, 1291, 1312, 1400
BstMWI	GCNNNNNNNGC	7 cut(s)	15, 24, 33, 327, 440, 926, 1267
BstNI	CCWGG	1 cut(s)	107
BstSCI	CCNGG	4 cut(s)	105, 560, 561, 1053
BstSFI	CTRYAG	1 cut(s)	1027
BstUI	CGCG	5 cut(s)	9, 14, 16, 804, 1336
BstV1I	GCAGC	2 cut(s)	443, 586
BstX2I	RGATCY	1 cut(s)	1400
BstYI	RGATCY	1 cut(s)	1400
Bsu15I	ATCGAT	2 cut(s)	63, 201
BsuI	GTATCC	1 cut(s)	1002
BsuRI	GGCC	1 cut(s)	356
BsuTUI	ATCGAT	2 cut(s)	63, 201
BtgI	CCRYGG	2 cut(s)	37, 458
BtrI	CACGTC	1 cut(s)	1364
BtsCI	GGATG	4 cut(s)	379, 676, 799, 1228
BtsI	GCAGTG	1 cut(s)	948
BtsIMutI	CAGTG	5 cut(s)	321, 757, 769, 948, 1341
Cac8I	GCNNGC	2 cut(s)	16, 44
CfoI	GCGC	4 cut(s)	11, 16, 18, 1336
Cfr10I	RCCGGY	1 cut(s)	356
Cfr13I	GGNCC	1 cut(s)	462
Cfr9I	CCCGGG	1 cut(s)	561
ClaI	ATCGAT	2 cut(s)	63, 201
Csp6I	GTAC	4 cut(s)	756, 851, 1194, 1235
CviAII	CATG	7 cut(s)	38, 241, 310, 459, 694, 1243, 1405
CviQI	GTAC	4 cut(s)	756, 851, 1194, 1235
DdeI	CTNAG	2 cut(s)	643, 703
DpnI	GATC	7 cut(s)	62, 200, 384, 420, 1293, 1314, 1402
DpnII	GATC	7 cut(s)	60, 198, 382, 418, 1291, 1312, 1400
DrdI	GACNNNNNNGTC	1 cut(s)	305
DseDI	GACNNNNNNGTC	1 cut(s)	305
Eam1104I	CTCTTC	2 cut(s)	532, 1206
EarI	CTCTTC	2 cut(s)	532, 1206
Eco130I	CCWWGG	2 cut(s)	37, 458
Eco31I	GGTCTC	2 cut(s)	47, 580
Eco47I	GGWCC	1 cut(s)	462
Eco57I	CTGAAG	1 cut(s)	880
Eco88I	CYCGRG	2 cut(s)	187, 561
EcoRII	CCWGG	1 cut(s)	105
EcoT14I	CCWWGG	2 cut(s)	37, 458
ErhI	CCWWGG	2 cut(s)	37, 458
FaeI	CATG	7 cut(s)	41, 244, 313, 462, 697, 1246, 1408
FaqI	GGGAC	2 cut(s)	44, 661
FatI	CATG	7 cut(s)	37, 240, 309, 458, 693, 1242, 1404
FbaI	TGATCA	1 cut(s)	1312
FblI	GTMKAC	1 cut(s)	1368
Fnu4HI	GCNGC	4 cut(s)	12, 19, 432, 600
FokI	GGATG	4 cut(s)	386, 683, 806, 1235
Fsp4HI	GCNGC	4 cut(s)	12, 19, 432, 600
FspBI	CTAG	5 cut(s)	471, 579, 591, 656, 1148
GlaI	GCGC	4 cut(s)	10, 15, 17, 1335
GluI	GCNGC	4 cut(s)	12, 19, 432, 600
GsaI	CCCAGC	1 cut(s)	31
GsuI	CTGGAG	2 cut(s)	780, 1024
HaeIII	GGCC	1 cut(s)	356
HapII	CCGG	3 cut(s)	357, 562, 1054
HhaI	GCGC	4 cut(s)	11, 16, 18, 1336
Hin1II	CATG	7 cut(s)	41, 244, 313, 462, 697, 1246, 1408
Hin6I	GCGC	4 cut(s)	9, 14, 16, 1334
HinP1I	GCGC	4 cut(s)	9, 14, 16, 1334
HindIII	AAGCTT	2 cut(s)	1259, 1268
HinfI	GANTC	4 cut(s)	557, 639, 1134, 1247
HpaII	CCGG	3 cut(s)	357, 562, 1054
HphI	GGTGA	4 cut(s)	428, 758, 800, 1238
Hpy166II	GTNNAC	3 cut(s)	292, 769, 1369
Hpy188I	TCNGA	5 cut(s)	102, 382, 706, 730, 1291
Hpy188III	TCNNGA	8 cut(s)	82, 208, 386, 422, 534, 972, 1112, 1435
Hpy8I	GTNNAC	3 cut(s)	292, 769, 1369
Hpy99I	CGWCG	2 cut(s)	734, 1365
HpyAV	CCTTC	7 cut(s)	166, 476, 508, 661, 907, 951, 1117
HpyCH4III	ACNGT	3 cut(s)	611, 752, 773
HpyCH4IV	ACGT	1 cut(s)	1363
HpyCH4V	TGCA	6 cut(s)	330, 522, 782, 815, 1157, 1297
HpyF10VI	GCNNNNNNNGC	7 cut(s)	15, 24, 33, 327, 440, 926, 1267
HpyF3I	CTNAG	2 cut(s)	643, 703
HpySE526I	ACGT	1 cut(s)	1363
Hsp92II	CATG	7 cut(s)	41, 244, 313, 462, 697, 1246, 1408
HspAI	GCGC	4 cut(s)	9, 14, 16, 1334
Ksp22I	TGATCA	1 cut(s)	1312
Kzo9I	GATC	7 cut(s)	60, 198, 382, 418, 1291, 1312, 1400
LmnI	GCTCC	5 cut(s)	138, 596, 781, 1060, 1444
Lsp1109I	GCAGC	2 cut(s)	443, 586
LweI	GCATC	1 cut(s)	106
MaeI	CTAG	5 cut(s)	471, 579, 591, 656, 1148
MaeII	ACGT	1 cut(s)	1363
MaeIII	GTNAC	4 cut(s)	3, 145, 178, 746
MalI	GATC	7 cut(s)	62, 200, 384, 420, 1293, 1314, 1402
MbiI	CCGCTC	1 cut(s)	340
MboI	GATC	7 cut(s)	60, 198, 382, 418, 1291, 1312, 1400
MboII	GAAGA	4 cut(s)	140, 549, 1193, 1349
MfeI	CAATTG	2 cut(s)	408, 1141
MflI	RGATCY	1 cut(s)	1400
MhlI	GDGCHC	1 cut(s)	778
MluCI	AATT	8 cut(s)	111, 269, 346, 408, 475, 499, 1141, 1346
MmeI	TCCRAC	2 cut(s)	612, 753
MseI	TTAA	4 cut(s)	503, 741, 966, 1281
MslI	CAYNNNNRTG	1 cut(s)	372
MspI	CCGG	3 cut(s)	357, 562, 1054
MspR9I	CCNGG	4 cut(s)	107, 562, 563, 1055
MteI	GCGCNGCGC	1 cut(s)	12
MunI	CAATTG	2 cut(s)	408, 1141
MvaI	CCWGG	1 cut(s)	107
MvnI	CGCG	5 cut(s)	9, 14, 16, 804, 1336
MwoI	GCNNNNNNNGC	7 cut(s)	15, 24, 33, 327, 440, 926, 1267
NciI	CCSGG	3 cut(s)	562, 563, 1055
NcoI	CCATGG	2 cut(s)	37, 458
NdeII	GATC	7 cut(s)	60, 198, 382, 418, 1291, 1312, 1400
NlaIII	CATG	7 cut(s)	41, 244, 313, 462, 697, 1246, 1408
NlaIV	GGNNCC	4 cut(s)	464, 976, 1110, 1402
NmuCI	GTSAC	2 cut(s)	3, 746
PauI	GCGCGC	1 cut(s)	14
PfeI	GAWTC	4 cut(s)	557, 639, 1134, 1247
PkrI	GCNGC	4 cut(s)	13, 20, 433, 601
PsiI	TTATAA	1 cut(s)	219
Psp6I	CCWGG	1 cut(s)	105
PspFI	CCCAGC	1 cut(s)	27
PspGI	CCWGG	1 cut(s)	105
PspN4I	GGNNCC	4 cut(s)	464, 976, 1110, 1402
PspPI	GGNCC	1 cut(s)	462
PsuI	RGATCY	1 cut(s)	1400
PteI	GCGCGC	1 cut(s)	14
RsaI	GTAC	4 cut(s)	757, 852, 1195, 1236
RsaNI	GTAC	4 cut(s)	756, 851, 1194, 1235
RseI	CAYNNNNRTG	1 cut(s)	372
SaqAI	TTAA	4 cut(s)	503, 741, 966, 1281
SatI	GCNGC	4 cut(s)	12, 19, 432, 600
Sau3AI	GATC	7 cut(s)	60, 198, 382, 418, 1291, 1312, 1400
Sau96I	GGNCC	1 cut(s)	462
ScaI	AGTACT	1 cut(s)	1236
ScrFI	CCNGG	4 cut(s)	107, 562, 563, 1055
SduI	GDGCHC	1 cut(s)	778
SfaNI	GCATC	1 cut(s)	106
SfcI	CTRYAG	1 cut(s)	1027
SinI	GGWCC	1 cut(s)	462
SmaI	CCCGGG	1 cut(s)	563
SmiMI	CAYNNNNRTG	1 cut(s)	372
SpeI	ACTAGT	1 cut(s)	655
Sse9I	AATT	8 cut(s)	111, 269, 346, 408, 475, 499, 1141, 1346
SsiI	CCGC	7 cut(s)	12, 19, 193, 338, 802, 1169, 1300
SspMI	CTAG	5 cut(s)	471, 579, 591, 656, 1148
StyD4I	CCNGG	4 cut(s)	105, 560, 561, 1053
StyI	CCWWGG	2 cut(s)	37, 458
TaaI	ACNGT	3 cut(s)	611, 752, 773
TaiI	ACGT	1 cut(s)	1366
TaqI	TCGA	6 cut(s)	63, 151, 201, 718, 1360, 1434
TaqII	GACCGA	1 cut(s)	71
TasI	AATT	8 cut(s)	111, 269, 346, 408, 475, 499, 1141, 1346
TatI	WGTACW	1 cut(s)	1234
TauI	GCSGC	2 cut(s)	14, 21
TfiI	GAWTC	4 cut(s)	557, 639, 1134, 1247
Tru1I	TTAA	4 cut(s)	503, 741, 966, 1281
Tru9I	TTAA	4 cut(s)	503, 741, 966, 1281
TscAI	CASTG	5 cut(s)	328, 757, 776, 948, 1348
TseFI	GTSAC	2 cut(s)	3, 746
TseI	GCWGC	2 cut(s)	431, 599
Tsp45I	GTSAC	2 cut(s)	3, 746
TspDTI	ATGAA	7 cut(s)	141, 229, 291, 558, 639, 682, 834
TspMI	CCCGGG	1 cut(s)	561
TspRI	CASTG	5 cut(s)	328, 757, 776, 948, 1348
VpaK11BI	GGWCC	1 cut(s)	462
XapI	RAATTY	2 cut(s)	499, 1346
XmaI	CCCGGG	1 cut(s)	561
XmiI	GTMKAC	1 cut(s)	1368
XspI	CTAG	5 cut(s)	471, 579, 591, 656, 1148
ZrmI	AGTACT	1 cut(s)	1236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.