Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0022774

Physical Location & Seq

Genomic Coordinates Forward (+)

74771682 .. 74807847

Gene Structure 36166 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG469500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1323 bp

ATGCCTGCAAAGAGGCATTCCTATGCTCAAAATGGTTGCTACCCTGAGGAGAGGGAACTGATTTTTGATATAGATATGTCAGATTACAATGATGTTAGATACTGCTGCTCAGGAGCTGATCTGTGCCTGGAATGCTGGCCGCTTATGACAATTGCTATCAAAGTGACTGACACTGCCCTTCGAGATGACCTTGGTTTCAATCACATTCCTAGGTATATAGTGGCCGACGTGGTGTTCATTGTTGGGCTCACAAATGAACTGAGAGCAGCTATTGCTGACTATTTCGGCAACGAAAACAGTCATAAAAAGTTTTCTTTGATGGGCCCTGGTCTTCATCCATTCCTTGTGAGATCATATACTGAAGTTCTTAAGGATTTCTTTGAGAGGACATCGTTTTCCAGTCAAAAGATATTTTATACTCTCCGGGGAAAGTGGCAAGATAATAGACGAACCTTCAATGCAAAAGAAGACATTAATATTGCTCGTTGGGAGCAGCTGAAACATTTGCTGCAATCTGGAAAACAGAAGATGCAAGGACTGCCTAGATGCGTGGAGGAGATTGTGTTCTCTTATACCTACCCAAGACTTGACATGGAGGTTTCAAAACATATGAACCATTTATTAAAGGCATCATTCTGTGTATATCCAAAGACGGGTCGTGTTTGTGTTCCGATTGATAATTTGATATCACCCGTTGCGAGGAGTTTGATCCTACTGCAGTTCCAACCCTTTCCGCTGAATGTGATGAGCTTCTCTCGGACTCGTTTCCATACAGAGAGATCCACAATGGAGTATTGTGGGAAGTTGACGGAAAGGTATGTGTGGGTTGTTCAAGGAGCAGTTGATGTAGACATCGGTGCAAATCCTTCTGCTGAAGGTGCAGATGCTGATGAGGGTGTTGATGATCAAACTGTTAAGGTGGTGGACATTGTTGACACTTTCAGACTTCAGGAGCAACCTCCTTTTGATAAGAAGCAGTTTGTCACATGGGTGAAGAGGTACATCAAATTGCTGACCCCAAAGCTTGAGGGAGAGCATCAGGAGACATTTAAGAAGAACATTGAGGGAGCAACCAAGTTTCTGCTTTCCAAGCTCAGTGACCTCCAGTTGCCCAAACTGAGACAACTATGTAGTCCCCAGCCCAAATGGTCCCATAATTTCCGCGCAAAAAGCAAACCGTTTGAATTTCATACCAAATCCCAAATCATAGCTACAAAATCCTCCCAATTCCAGTTCCCATCGCCCCAAATTCGAACACATCCTTCATCTTCGTCTTCAGTGATATTCAATTCAGCCACAAGGCAACAGAAACAGATCATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003824 GO:0003896 GO:0003899 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0032993 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097159 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_primase_S

Protein Analysis

440

Amino Acids

50.76

Weight (kDa)

8.91

Isoelectric Point (pI)

46.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_primase_S	PF01896	22 - 226	4.5e-27	DNA primase small subunit
TCTP	PF00838	275 - 369	1e-22	Translationally controlled tumour protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0022774)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr5g0068871
rosa_samantha	Rh5AG451100 Rh5BG469500 Rh5CG491900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	849
AccII	CGCG	1 cut(s)	1164
AciI	CCGC	3 cut(s)	140, 734, 1162
AclWI	GGATC	2 cut(s)	703, 774
AcoI	YGGCCR	2 cut(s)	137, 222
AcsI	RAATTY	2 cut(s)	1184, 1248
AcuI	CTGAAG	4 cut(s)	381, 894, 932, 1260
AfaI	GTAC	1 cut(s)	1001
AfiI	CCNNNNNNNGG	2 cut(s)	653, 699
AflII	CTTAAG	1 cut(s)	368
AgsI	TTSAA	6 cut(s)	199, 457, 603, 833, 1184, 1288
AjiI	CACGTC	1 cut(s)	229
AjnI	CCWGG	2 cut(s)	126, 325
AleI	CACNNNNGTG	1 cut(s)	989
AloI	GAACNNNNNNTCC	2 cut(s)	548, 580
AluBI	AGCT	7 cut(s)	116, 269, 496, 750, 1024, 1093, 1211
AluI	AGCT	7 cut(s)	116, 269, 496, 750, 1024, 1093, 1211
Alw26I	GTCTC	2 cut(s)	1037, 1114
AlwI	GGATC	2 cut(s)	703, 774
AlwNI	CAGNNNCTG	2 cut(s)	116, 887
AoxI	GGCC	3 cut(s)	137, 222, 322
ApaI	GGGCCC	1 cut(s)	326
ApeKI	GCWGC	4 cut(s)	105, 266, 493, 508
ApoI	RAATTY	2 cut(s)	1184, 1248
AseI	ATTAAT	1 cut(s)	474
AspA2I	CCTAGG	1 cut(s)	209
AspLEI	GCGC	1 cut(s)	1166
AspS9I	GGNCC	3 cut(s)	322, 323, 1149
AsuC2I	CCSGG	1 cut(s)	425
AsuHPI	GGTGA	2 cut(s)	681, 1003
AsuII	TTCGAA	1 cut(s)	1252
AvaII	GGWCC	1 cut(s)	1149
AvrII	CCTAGG	1 cut(s)	209
AxyI	CCTNAGG	1 cut(s)	45
BaeGI	GKGCMC	1 cut(s)	326
BanII	GRGCYC	2 cut(s)	249, 326
BbsI	GAAGAC	3 cut(s)	323, 474, 1266
BbvI	GCAGC	4 cut(s)	92, 278, 495, 505
BccI	CCATC	2 cut(s)	313, 1246
BciT130I	CCWGG	2 cut(s)	128, 327
BclI	TGATCA	1 cut(s)	904
BcnI	CCSGG	1 cut(s)	425
BcoDI	GTCTC	2 cut(s)	1037, 1114
BfaI	CTAG	2 cut(s)	210, 543
BfmI	CTRYAG	1 cut(s)	716
BfrI	CTTAAG	1 cut(s)	368
BisI	GCNGC	5 cut(s)	106, 140, 267, 494, 509
BlnI	CCTAGG	1 cut(s)	209
BlsI	GCNGC	5 cut(s)	107, 141, 268, 495, 510
Bme1390I	CCNGG	3 cut(s)	128, 327, 425
Bme18I	GGWCC	1 cut(s)	1149
BmgBI	CACGTC	1 cut(s)	229
BmgT120I	GGNCC	3 cut(s)	322, 323, 1149
BmiI	GGNNCC	2 cut(s)	324, 1151
BmrFI	CCNGG	3 cut(s)	128, 327, 425
BmsI	GCATC	5 cut(s)	519, 536, 638, 874, 1045
BpiI	GAAGAC	3 cut(s)	323, 474, 1266
BplI	GAGNNNNNCTC	2 cut(s)	739, 771
BpmI	CTGGAG	1 cut(s)	1088
Bpu10I	CCTNAGC	1 cut(s)	109
Bpu14I	TTCGAA	1 cut(s)	1252
BpuEI	CTTGAG	1 cut(s)	1046
BpuMI	CCSGG	1 cut(s)	425
BsaJI	CCNNGG	4 cut(s)	190, 209, 325, 424
BsaXI	ACNNNNNCTCC	2 cut(s)	548, 578
Bsc4I	CCNNNNNNNGG	2 cut(s)	653, 699
Bse1I	ACTGG	3 cut(s)	399, 1105, 1231
Bse21I	CCTNAGG	1 cut(s)	45
BseBI	CCWGG	2 cut(s)	128, 327
BseDI	CCNNGG	4 cut(s)	190, 209, 325, 424
BseGI	GGATG	2 cut(s)	334, 1258
BseLI	CCNNNNNNNGG	2 cut(s)	653, 699
BseMII	CTCAG	5 cut(s)	36, 123, 251, 1108, 1109
BseNI	ACTGG	3 cut(s)	399, 1105, 1231
BseRI	GAGGAG	3 cut(s)	62, 569, 715
BseSI	GKGCMC	1 cut(s)	326
BseXI	GCAGC	4 cut(s)	92, 278, 495, 505
BseYI	CCCAGC	1 cut(s)	1137
BsgI	GTGCAG	1 cut(s)	900
Bsh1236I	CGCG	1 cut(s)	1164
BshFI	GGCC	3 cut(s)	139, 224, 324
BsiSI	CCGG	1 cut(s)	424
BslFI	GGGAC	2 cut(s)	1119, 1135
BslI	CCNNNNNNNGG	2 cut(s)	653, 699
BsmAI	GTCTC	2 cut(s)	1037, 1114
BsmFI	GGGAC	2 cut(s)	1119, 1135
BsmI	GAATGC	2 cut(s)	16, 137
BsnI	GGCC	3 cut(s)	139, 224, 324
Bsp119I	TTCGAA	1 cut(s)	1252
Bsp120I	GGGCCC	1 cut(s)	322
Bsp1286I	GDGCHC	2 cut(s)	249, 326
Bsp143I	GATC	6 cut(s)	118, 350, 708, 779, 904, 1314
BspACI	CCGC	3 cut(s)	140, 734, 1162
BspANI	GGCC	3 cut(s)	139, 224, 324
BspCNI	CTCAG	5 cut(s)	37, 122, 252, 1107, 1110
BspFNI	CGCG	1 cut(s)	1164
BspLI	GGNNCC	2 cut(s)	324, 1151
BspMAI	CTGCAG	1 cut(s)	720
BspPI	GGATC	2 cut(s)	703, 774
BspT104I	TTCGAA	1 cut(s)	1252
BspTI	CTTAAG	1 cut(s)	368
BsrI	ACTGG	3 cut(s)	399, 1105, 1231
BssECI	CCNNGG	4 cut(s)	190, 209, 325, 424
BssMI	GATC	6 cut(s)	118, 350, 708, 779, 904, 1314
BssT1I	CCWWGG	2 cut(s)	190, 209
Bst2UI	CCWGG	2 cut(s)	128, 327
Bst4CI	ACNGT	3 cut(s)	299, 913, 1179
Bst6I	CTCTTC	1 cut(s)	989
BstAFI	CTTAAG	1 cut(s)	368
BstAPI	GCANNNNNTGC	2 cut(s)	272, 538
BstBI	TTCGAA	1 cut(s)	1252
BstC8I	GCNNGC	2 cut(s)	6, 137
BstDEI	CTNAG	5 cut(s)	45, 109, 260, 1094, 1118
BstF5I	GGATG	2 cut(s)	334, 1258
BstFNI	CGCG	1 cut(s)	1164
BstHHI	GCGC	1 cut(s)	1166
BstKTI	GATC	6 cut(s)	121, 353, 711, 782, 907, 1317
BstMAI	GTCTC	2 cut(s)	1037, 1114
BstMBI	GATC	6 cut(s)	118, 350, 708, 779, 904, 1314
BstMWI	GCNNNNNNNGC	6 cut(s)	132, 272, 538, 878, 1090, 1170
BstNI	CCWGG	2 cut(s)	128, 327
BstSCI	CCNGG	3 cut(s)	126, 325, 423
BstSFI	CTRYAG	1 cut(s)	716
BstSLI	GKGCMC	1 cut(s)	326
BstUI	CGCG	1 cut(s)	1164
BstV1I	GCAGC	4 cut(s)	92, 278, 495, 505
BstV2I	GAAGAC	3 cut(s)	323, 474, 1266
BstX2I	RGATCY	1 cut(s)	779
BstYI	RGATCY	1 cut(s)	779
Bsu36I	CCTNAGG	1 cut(s)	45
BsuRI	GGCC	3 cut(s)	139, 224, 324
BtgZI	GCGATG	1 cut(s)	1224
BtrI	CACGTC	1 cut(s)	229
BtsCI	GGATG	2 cut(s)	334, 1258
BtsI	GCAGTG	1 cut(s)	171
BtsIMutI	CAGTG	3 cut(s)	171, 1102, 1284
Cac8I	GCNNGC	2 cut(s)	6, 137
CaiI	CAGNNNCTG	2 cut(s)	116, 887
CfoI	GCGC	1 cut(s)	1166
Cfr13I	GGNCC	3 cut(s)	322, 323, 1149
Csp6I	GTAC	1 cut(s)	1000
CviAII	CATG	2 cut(s)	592, 987
CviQI	GTAC	1 cut(s)	1000
DdeI	CTNAG	5 cut(s)	45, 109, 260, 1094, 1118
DpnI	GATC	6 cut(s)	120, 352, 710, 781, 906, 1316
DpnII	GATC	6 cut(s)	118, 350, 708, 779, 904, 1314
EaeI	YGGCCR	2 cut(s)	137, 222
Eam1104I	CTCTTC	1 cut(s)	989
EarI	CTCTTC	1 cut(s)	989
Eco130I	CCWWGG	2 cut(s)	190, 209
Eco24I	GRGCYC	2 cut(s)	249, 326
Eco32I	GATATC	1 cut(s)	687
Eco47I	GGWCC	1 cut(s)	1149
Eco57I	CTGAAG	4 cut(s)	381, 894, 932, 1260
Eco81I	CCTNAGG	1 cut(s)	45
EcoO109I	RGGNCCY	1 cut(s)	323
EcoRII	CCWGG	2 cut(s)	126, 325
EcoRV	GATATC	1 cut(s)	687
EcoT14I	CCWWGG	2 cut(s)	190, 209
EcoT38I	GRGCYC	2 cut(s)	249, 326
ErhI	CCWWGG	2 cut(s)	190, 209
FaeI	CATG	2 cut(s)	595, 990
FalI	AAGNNNNNCTT	2 cut(s)	362, 394
FaqI	GGGAC	2 cut(s)	1119, 1135
FatI	CATG	2 cut(s)	591, 986
FauNDI	CATATG	1 cut(s)	609
FbaI	TGATCA	1 cut(s)	904
FblI	GTMKAC	1 cut(s)	849
Fnu4HI	GCNGC	5 cut(s)	106, 140, 267, 494, 509
FokI	GGATG	2 cut(s)	321, 1245
FriOI	GRGCYC	2 cut(s)	249, 326
Fsp4HI	GCNGC	5 cut(s)	106, 140, 267, 494, 509
FspBI	CTAG	2 cut(s)	210, 543
GlaI	GCGC	1 cut(s)	1165
GluI	GCNGC	5 cut(s)	106, 140, 267, 494, 509
GsaI	CCCAGC	1 cut(s)	1141
GsuI	CTGGAG	1 cut(s)	1088
HaeIII	GGCC	3 cut(s)	139, 224, 324
HapII	CCGG	1 cut(s)	424
HhaI	GCGC	1 cut(s)	1166
Hin1II	CATG	2 cut(s)	595, 990
Hin6I	GCGC	1 cut(s)	1164
HinP1I	GCGC	1 cut(s)	1164
HincII	GTYRAC	2 cut(s)	807, 934
HindII	GTYRAC	2 cut(s)	807, 934
HindIII	AAGCTT	1 cut(s)	1022
HinfI	GANTC	1 cut(s)	760
HpaII	CCGG	1 cut(s)	424
HphI	GGTGA	2 cut(s)	681, 1003
Hpy166II	GTNNAC	4 cut(s)	807, 850, 925, 934
Hpy188I	TCNGA	4 cut(s)	82, 672, 759, 944
Hpy188III	TCNNGA	5 cut(s)	111, 182, 516, 950, 1040
Hpy8I	GTNNAC	4 cut(s)	807, 850, 925, 934
Hpy99I	CGWCG	1 cut(s)	230
HpyAV	CCTTC	5 cut(s)	188, 463, 869, 876, 1272
HpyCH4III	ACNGT	3 cut(s)	299, 913, 1179
HpyCH4IV	ACGT	1 cut(s)	228
HpyCH4V	TGCA	7 cut(s)	8, 461, 511, 532, 718, 860, 881
HpyF10VI	GCNNNNNNNGC	6 cut(s)	132, 272, 538, 878, 1090, 1170
HpyF3I	CTNAG	5 cut(s)	45, 109, 260, 1094, 1118
HpySE526I	ACGT	1 cut(s)	228
Hsp92II	CATG	2 cut(s)	595, 990
HspAI	GCGC	1 cut(s)	1164
Ksp22I	TGATCA	1 cut(s)	904
Kzo9I	GATC	6 cut(s)	118, 350, 708, 779, 904, 1314
LmnI	GCTCC	5 cut(s)	113, 490, 836, 952, 1067
Lsp1109I	GCAGC	4 cut(s)	92, 278, 495, 505
LweI	GCATC	5 cut(s)	519, 536, 638, 874, 1045
MaeI	CTAG	2 cut(s)	210, 543
MaeII	ACGT	1 cut(s)	228
MaeIII	GTNAC	3 cut(s)	163, 982, 1097
MalI	GATC	6 cut(s)	120, 352, 710, 781, 906, 1316
MboI	GATC	6 cut(s)	118, 350, 708, 779, 904, 1314
MboII	GAAGA	7 cut(s)	323, 479, 538, 1006, 1066, 1260, 1266
MfeI	CAATTG	1 cut(s)	150
MflI	RGATCY	1 cut(s)	779
MhlI	GDGCHC	2 cut(s)	249, 326
MluCI	AATT	8 cut(s)	150, 679, 1007, 1156, 1184, 1226, 1248, 1288
MlyI	GAGTC	1 cut(s)	754
MmeI	TCCRAC	1 cut(s)	748
MseI	TTAA	5 cut(s)	369, 474, 623, 915, 1050
MslI	CAYNNNNRTG	2 cut(s)	21, 989
MspA1I	CMGCKG	2 cut(s)	496, 736
MspCI	CTTAAG	1 cut(s)	368
MspI	CCGG	1 cut(s)	424
MspR9I	CCNGG	3 cut(s)	128, 327, 425
MunI	CAATTG	1 cut(s)	150
Mva1269I	GAATGC	2 cut(s)	16, 137
MvaI	CCWGG	2 cut(s)	128, 327
MvnI	CGCG	1 cut(s)	1164
MwoI	GCNNNNNNNGC	6 cut(s)	132, 272, 538, 878, 1090, 1170
NciI	CCSGG	1 cut(s)	425
NdeI	CATATG	1 cut(s)	609
NdeII	GATC	6 cut(s)	118, 350, 708, 779, 904, 1314
NlaIII	CATG	2 cut(s)	595, 990
NlaIV	GGNNCC	2 cut(s)	324, 1151
NmuCI	GTSAC	3 cut(s)	163, 982, 1097
NspV	TTCGAA	1 cut(s)	1252
OliI	CACNNNNGTG	1 cut(s)	989
PctI	GAATGC	2 cut(s)	16, 137
PkrI	GCNGC	5 cut(s)	107, 141, 268, 495, 510
PleI	GAGTC	1 cut(s)	754
PpsI	GAGTC	1 cut(s)	754
PshBI	ATTAAT	1 cut(s)	474
Psp6I	CCWGG	2 cut(s)	126, 325
PspFI	CCCAGC	1 cut(s)	1137
PspGI	CCWGG	2 cut(s)	126, 325
PspN4I	GGNNCC	2 cut(s)	324, 1151
PspOMI	GGGCCC	1 cut(s)	322
PspPI	GGNCC	3 cut(s)	322, 323, 1149
PstI	CTGCAG	1 cut(s)	720
PstNI	CAGNNNCTG	2 cut(s)	116, 887
PsuI	RGATCY	1 cut(s)	779
PvuII	CAGCTG	1 cut(s)	496
RsaI	GTAC	1 cut(s)	1001
RsaNI	GTAC	1 cut(s)	1000
RseI	CAYNNNNRTG	2 cut(s)	21, 989
SaqAI	TTAA	5 cut(s)	369, 474, 623, 915, 1050
SatI	GCNGC	5 cut(s)	106, 140, 267, 494, 509
Sau3AI	GATC	6 cut(s)	118, 350, 708, 779, 904, 1314
Sau96I	GGNCC	3 cut(s)	322, 323, 1149
SchI	GAGTC	1 cut(s)	754
ScrFI	CCNGG	3 cut(s)	128, 327, 425
SduI	GDGCHC	2 cut(s)	249, 326
SfaNI	GCATC	5 cut(s)	519, 536, 638, 874, 1045
SfcI	CTRYAG	1 cut(s)	716
SfuI	TTCGAA	1 cut(s)	1252
SinI	GGWCC	1 cut(s)	1149
SmiMI	CAYNNNNRTG	2 cut(s)	21, 989
SmlI	CTYRAG	2 cut(s)	368, 1025
SmoI	CTYRAG	2 cut(s)	368, 1025
Sse9I	AATT	8 cut(s)	150, 679, 1007, 1156, 1184, 1226, 1248, 1288
SsiI	CCGC	3 cut(s)	140, 734, 1162
SspI	AATATT	1 cut(s)	478
SspMI	CTAG	2 cut(s)	210, 543
StyD4I	CCNGG	3 cut(s)	126, 325, 423
StyI	CCWWGG	2 cut(s)	190, 209
TaaI	ACNGT	3 cut(s)	299, 913, 1179
TaiI	ACGT	1 cut(s)	231
TaqI	TCGA	2 cut(s)	181, 1252
TasI	AATT	8 cut(s)	150, 679, 1007, 1156, 1184, 1226, 1248, 1288
TauI	GCSGC	1 cut(s)	142
Tru1I	TTAA	5 cut(s)	369, 474, 623, 915, 1050
Tru9I	TTAA	5 cut(s)	369, 474, 623, 915, 1050
TscAI	CASTG	3 cut(s)	178, 1102, 1284
TseFI	GTSAC	3 cut(s)	163, 982, 1097
TseI	GCWGC	4 cut(s)	105, 266, 493, 508
Tsp45I	GTSAC	3 cut(s)	163, 982, 1097
TspDTI	ATGAA	6 cut(s)	226, 270, 323, 626, 1178, 1254
TspGWI	ACGGA	1 cut(s)	824
TspRI	CASTG	3 cut(s)	178, 1102, 1284
Vha464I	CTTAAG	1 cut(s)	368
VpaK11BI	GGWCC	1 cut(s)	1149
VspI	ATTAAT	1 cut(s)	474
XapI	RAATTY	2 cut(s)	1184, 1248
XmaJI	CCTAGG	1 cut(s)	209
XmiI	GTMKAC	1 cut(s)	849
XspI	CTAG	2 cut(s)	210, 543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.