Rh5CG187800

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0020253
Physical Location & Seq
Reverse (-)
18721935 .. 18726496
4562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG187800.1

Sequence Viewer

Length: 729 bp
ATGTCTTCAATCCCCAGAGACGAAGCAGCCTCGATTCAATGGCAGAAGGGCGATTTGATCGGTTCCGGGGCCTTTGGCCGTGTTTACATGGGCATGAATACCGAATCCGGAGAGCTTATTGCTGCAAAAGAGGCTCATACCAAGGTGCTTCAAGCTTTAGAAAACCTGTCGCATCCGAAAATTATTAGATGTTTAGGAACCGAGAGGGAGGATGAAACCTTAACTATGCTGTTGGAATATGTACCTGGAGGATCCATACAAGCACTATTGGAGAAATTTGGATCTTTCTCTGTTCCTGCAGCCGTTTCAGGTGCAAAGCCTACAAGGGGTACACCATACTGGATGGCTCCTGAAGTCATTTGTGGGACTGGTCATGACTTGGAACCAACTGTAAGGCCATCCGCATCTGAGTTGCTTAAGGCACGGCAGTTATGGCAAAAAGGTGCAGGGCTACAACTAGTGGATCCAAAACTGGAAGTTTCTCGTATTGACAAACATCAACTGTTAAGATGCATCCATGTTGGTCTGCTGTGCGCGGAAGAAAATGAAGATGACCGGCCCACCATGTCAGATGCGATATCTATGTTGAAAAATGAAGTGCCTTTGCCTGAACCAACAATGCCAGCATTTTCTAGATACAATAATTGGACAACTGCTAGTATGGAAACTATGTGCATATATGGTTTATCAATAAACAGTATGACGATTTCCGACTTTGGTGGACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

242

Amino Acids

26.51

Weight (kDa)

5.32

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 15 - 97 4.2e-11 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 17 - 92 2.3e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020253)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43930 AT2G43930
rosa_chinensis RchiOBHm_Chr2g0104591
rosa_roxburghii Rroxscaffold_4G00302700
rosa_rugosa Rorug01G0229900
rosa_samantha Rh5CG187800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 536
AccIII TCCGGA 1 cut(s) 107
AciI CCGC 2 cut(s) 402, 536
AclWI GGATC 5 cut(s) 246, 259, 289, 458, 471
AcoI YGGCCR 1 cut(s) 76
AcsI RAATTY 1 cut(s) 275
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 2 cut(s) 243, 331
AfiI CCNNNNNNNGG 1 cut(s) 326
AflII CTTAAG 1 cut(s) 416
AgsI TTSAA 4 cut(s) 9, 38, 152, 589
AhlI ACTAGT 1 cut(s) 457
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 2 cut(s) 115, 155
AluI AGCT 2 cut(s) 115, 155
Alw26I GTCTC 1 cut(s) 12
AlwI GGATC 5 cut(s) 246, 259, 289, 458, 471
Aor13HI TCCGGA 1 cut(s) 107
AoxI GGCC 4 cut(s) 69, 76, 395, 557
ApeKI GCWGC 3 cut(s) 26, 122, 299
ApoI RAATTY 1 cut(s) 275
AspLEI GCGC 1 cut(s) 536
AspS9I GGNCC 2 cut(s) 69, 558
AsuC2I CCSGG 1 cut(s) 67
BamHI GGATCC 2 cut(s) 251, 463
BbvI GCAGC 3 cut(s) 38, 109, 311
BccI CCATC 2 cut(s) 337, 406
BceAI ACGGC 3 cut(s) 63, 287, 440
BciT130I CCWGG 1 cut(s) 246
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 1 cut(s) 12
BcuI ACTAGT 1 cut(s) 457
BfaI CTAG 3 cut(s) 458, 633, 657
BfmI CTRYAG 1 cut(s) 297
BfrI CTTAAG 1 cut(s) 416
BisI GCNGC 3 cut(s) 27, 123, 300
BlsI GCNGC 3 cut(s) 28, 124, 301
Bme1390I CCNGG 2 cut(s) 67, 246
BmgT120I GGNCC 2 cut(s) 69, 558
BmiI GGNNCC 7 cut(s) 64, 70, 199, 253, 348, 384, 465
BmrFI CCNGG 2 cut(s) 67, 246
BmsI GCATC 5 cut(s) 181, 413, 500, 522, 562
BpmI CTGGAG 1 cut(s) 267
BpuMI CCSGG 1 cut(s) 67
BsaJI CCNNGG 2 cut(s) 66, 141
BsaWI WCCGGW 1 cut(s) 107
Bsc4I CCNNNNNNNGG 1 cut(s) 326
Bse118I RCCGGY 1 cut(s) 555
Bse1I ACTGG 3 cut(s) 344, 373, 477
BseAI TCCGGA 1 cut(s) 107
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 2 cut(s) 66, 141
BseGI GGATG 5 cut(s) 172, 217, 348, 398, 513
BseLI CCNNNNNNNGG 1 cut(s) 326
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 3 cut(s) 344, 373, 477
BseXI GCAGC 3 cut(s) 38, 109, 311
BsgI GTGCAG 1 cut(s) 465
Bsh1236I CGCG 1 cut(s) 536
BshFI GGCC 4 cut(s) 71, 78, 397, 559
BsiSI CCGG 3 cut(s) 66, 108, 556
BslFI GGGAC 1 cut(s) 379
BslI CCNNNNNNNGG 1 cut(s) 326
BsmAI GTCTC 1 cut(s) 12
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 1 cut(s) 379
BsnI GGCC 4 cut(s) 71, 78, 397, 559
Bsp13I TCCGGA 1 cut(s) 107
Bsp143I GATC 4 cut(s) 57, 251, 281, 463
BspACI CCGC 2 cut(s) 402, 536
BspANI GGCC 4 cut(s) 71, 78, 397, 559
BspCNI CTCAG 1 cut(s) 400
BspEI TCCGGA 1 cut(s) 107
BspFNI CGCG 1 cut(s) 536
BspHI TCATGA 1 cut(s) 373
BspLI GGNNCC 7 cut(s) 64, 70, 199, 253, 348, 384, 465
BspMAI CTGCAG 1 cut(s) 301
BspPI GGATC 5 cut(s) 246, 259, 289, 458, 471
BspTI CTTAAG 1 cut(s) 416
BsrFI RCCGGY 1 cut(s) 555
BsrI ACTGG 3 cut(s) 344, 373, 477
BssAI RCCGGY 1 cut(s) 555
BssECI CCNNGG 2 cut(s) 66, 141
BssMI GATC 4 cut(s) 57, 251, 281, 463
BssT1I CCWWGG 1 cut(s) 141
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 3 cut(s) 391, 504, 698
BstAFI CTTAAG 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 624
BstDEI CTNAG 1 cut(s) 408
BstF5I GGATG 5 cut(s) 172, 217, 348, 398, 513
BstFNI CGCG 1 cut(s) 536
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 4 cut(s) 60, 254, 284, 466
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 4 cut(s) 57, 251, 281, 463
BstMWI GCNNNNNNNGC 2 cut(s) 131, 433
BstNI CCWGG 1 cut(s) 246
BstSCI CCNGG 2 cut(s) 65, 244
BstSFI CTRYAG 1 cut(s) 297
BstUI CGCG 1 cut(s) 536
BstV1I GCAGC 3 cut(s) 38, 109, 311
BstX2I RGATCY 3 cut(s) 251, 281, 463
BstYI RGATCY 3 cut(s) 251, 281, 463
BsuRI GGCC 4 cut(s) 71, 78, 397, 559
BtsCI GGATG 5 cut(s) 172, 217, 348, 398, 513
Cac8I GCNNGC 1 cut(s) 624
CciI TCATGA 1 cut(s) 373
CfoI GCGC 1 cut(s) 536
Cfr10I RCCGGY 1 cut(s) 555
Cfr13I GGNCC 2 cut(s) 69, 558
Csp6I GTAC 2 cut(s) 242, 330
CviAII CATG 5 cut(s) 88, 94, 374, 518, 565
CviQI GTAC 2 cut(s) 242, 330
DdeI CTNAG 1 cut(s) 408
DpnI GATC 4 cut(s) 59, 253, 283, 465
DpnII GATC 4 cut(s) 57, 251, 281, 463
EaeI YGGCCR 1 cut(s) 76
Eco130I CCWWGG 1 cut(s) 141
Eco32I GATATC 1 cut(s) 579
Eco57I CTGAAG 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 69
EcoRII CCWGG 1 cut(s) 244
EcoRV GATATC 1 cut(s) 579
EcoT14I CCWWGG 1 cut(s) 141
EcoT22I ATGCAT 1 cut(s) 515
ErhI CCWWGG 1 cut(s) 141
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 5 cut(s) 91, 97, 377, 521, 568
FaqI GGGAC 1 cut(s) 379
FatI CATG 5 cut(s) 87, 93, 373, 517, 564
Fnu4HI GCNGC 3 cut(s) 27, 123, 300
FokI GGATG 5 cut(s) 159, 224, 355, 385, 500
Fsp4HI GCNGC 3 cut(s) 27, 123, 300
FspBI CTAG 3 cut(s) 458, 633, 657
GlaI GCGC 1 cut(s) 535
GluI GCNGC 3 cut(s) 27, 123, 300
GsuI CTGGAG 1 cut(s) 267
HaeIII GGCC 4 cut(s) 71, 78, 397, 559
HapII CCGG 3 cut(s) 66, 108, 556
HhaI GCGC 1 cut(s) 536
Hin1II CATG 5 cut(s) 91, 97, 377, 521, 568
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HindIII AAGCTT 1 cut(s) 153
HinfI GANTC 2 cut(s) 34, 104
HpaII CCGG 3 cut(s) 66, 108, 556
Hpy166II GTNNAC 3 cut(s) 85, 332, 722
Hpy188I TCNGA 4 cut(s) 177, 409, 571, 712
Hpy188III TCNNGA 4 cut(s) 108, 350, 374, 633
Hpy8I GTNNAC 3 cut(s) 85, 332, 722
HpyAV CCTTC 1 cut(s) 40
HpyCH4III ACNGT 3 cut(s) 391, 504, 698
HpyCH4IV ACGT 1 cut(s) 724
HpyCH4V TGCA 6 cut(s) 125, 299, 314, 446, 513, 675
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 433
HpyF3I CTNAG 1 cut(s) 408
HpySE526I ACGT 1 cut(s) 724
Hsp92II CATG 5 cut(s) 91, 97, 377, 521, 568
HspAI GCGC 1 cut(s) 534
Kpn2I TCCGGA 1 cut(s) 107
Kzo9I GATC 4 cut(s) 57, 251, 281, 463
LmnI GCTCC 1 cut(s) 352
Lsp1109I GCAGC 3 cut(s) 38, 109, 311
LweI GCATC 5 cut(s) 181, 413, 500, 522, 562
MaeI CTAG 3 cut(s) 458, 633, 657
MaeII ACGT 1 cut(s) 724
MalI GATC 4 cut(s) 59, 253, 283, 465
MboI GATC 4 cut(s) 57, 251, 281, 463
MboII GAAGA 2 cut(s) 551, 560
MflI RGATCY 3 cut(s) 251, 281, 463
MluCI AATT 3 cut(s) 180, 275, 643
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 5 cut(s) 40, 124, 198, 202, 242
Mph1103I ATGCAT 1 cut(s) 515
MroI TCCGGA 1 cut(s) 107
MseI TTAA 4 cut(s) 221, 417, 506, 727
MslI CAYNNNNRTG 1 cut(s) 92
MspCI CTTAAG 1 cut(s) 416
MspI CCGG 3 cut(s) 66, 108, 556
MspR9I CCNGG 2 cut(s) 67, 246
MvaI CCWGG 1 cut(s) 246
MvnI CGCG 1 cut(s) 536
MwoI GCNNNNNNNGC 2 cut(s) 131, 433
NciI CCSGG 1 cut(s) 67
NdeII GATC 4 cut(s) 57, 251, 281, 463
NlaIII CATG 5 cut(s) 91, 97, 377, 521, 568
NlaIV GGNNCC 7 cut(s) 64, 70, 199, 253, 348, 384, 465
NsiI ATGCAT 1 cut(s) 515
PagI TCATGA 1 cut(s) 373
PfeI GAWTC 2 cut(s) 34, 104
PkrI GCNGC 3 cut(s) 28, 124, 301
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 7 cut(s) 64, 70, 199, 253, 348, 384, 465
PspPI GGNCC 2 cut(s) 69, 558
PstI CTGCAG 1 cut(s) 301
PsuI RGATCY 3 cut(s) 251, 281, 463
RsaI GTAC 2 cut(s) 243, 331
RsaNI GTAC 2 cut(s) 242, 330
RseI CAYNNNNRTG 1 cut(s) 92
SaqAI TTAA 4 cut(s) 221, 417, 506, 727
SatI GCNGC 3 cut(s) 27, 123, 300
Sau3AI GATC 4 cut(s) 57, 251, 281, 463
Sau96I GGNCC 2 cut(s) 69, 558
ScrFI CCNGG 2 cut(s) 67, 246
SetI ASST 9 cut(s) 117, 147, 157, 168, 221, 247, 313, 445, 727
SfaNI GCATC 5 cut(s) 181, 413, 500, 522, 562
SfcI CTRYAG 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
SpeI ACTAGT 1 cut(s) 457
Sse9I AATT 3 cut(s) 180, 275, 643
SsiI CCGC 2 cut(s) 402, 536
SspMI CTAG 3 cut(s) 458, 633, 657
StyD4I CCNGG 2 cut(s) 65, 244
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 3 cut(s) 391, 504, 698
TaiI ACGT 1 cut(s) 727
TaqI TCGA 1 cut(s) 32
TasI AATT 3 cut(s) 180, 275, 643
TfiI GAWTC 2 cut(s) 34, 104
Tru1I TTAA 4 cut(s) 221, 417, 506, 727
Tru9I TTAA 4 cut(s) 221, 417, 506, 727
TseI GCWGC 3 cut(s) 26, 122, 299
TspDTI ATGAA 4 cut(s) 110, 228, 561, 609
Vha464I CTTAAG 1 cut(s) 416
XapI RAATTY 1 cut(s) 275
XbaI TCTAGA 1 cut(s) 632
XspI CTAG 3 cut(s) 458, 633, 657
Zsp2I ATGCAT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.