Rh5CG219900

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0021041
Physical Location & Seq
Forward (+)
22354852 .. 22358247
3396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG219900.1

Sequence Viewer

Length: 660 bp
ATGAGCCCATTAGGTATAAGATACTGGGCCACTAAAGCTCAACATAGCGCTCACATTTATACGCTTCACAAAGATTGTGAAAATGTCTTTGGAGCTCTCATTCGCAGAGGACTATGCTTTCGTTTTTCAGCAGCAAATTCGATCTTTCAGGCTTGCTCTGTGCCTCCTTTTGATAGTTTGAGCAAATTGAGGCCGGTTCATTCTAGGATCTCTTACTGGTGGAGAACTTTAATGAGGATGCTAGAGAGATCACCTAACCTAGAGTGTCTTGCAATAGACGACTGCTCTTATCAAACTCGAAGACAGCTGGAAGAGGAGGTGATTGAACATGCTGACAAAGGAGCAGAGGTGATTGAACAAGCTGAGAATGGACCGGAGGTGATTGAACGAGCTGAGAATGGACTGGAGGTGATTGATCTTAGAGAGCAAATAGCAGCACTAGTACGTACTCTGAACTGGGTTCCACCAGATTCTATTCCCAAATGTTTGCCGTCACACCTGAAGACTATCTCCATTAACGGATTCTATGGAAAGGGAACGATTGGATATCTGGATGAGATGGAGTTGATCAAGTATTTATTAAAGAATGGTAGAGTTCTGGAGAAGATGACTATTTATACCCTGCGAGTAGCAACCGTTGATGAAGACTTGTCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

25.04

Weight (kDa)

6.09

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBD PF08387 156 - 205 2.4e-13 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021041)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g18010 FvH4_3g18010
rosa_laevigata RLG00000033101
rosa_samantha Rh5CG219900 Rh5DG202700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 215
AcsI RAATTY 1 cut(s) 136
AcuI CTGAAG 1 cut(s) 521
AfaI GTAC 2 cut(s) 444, 448
AfeI AGCGCT 1 cut(s) 49
AgsI TTSAA 3 cut(s) 326, 356, 386
AhlI ACTAGT 1 cut(s) 439
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AluBI AGCT 5 cut(s) 38, 95, 307, 362, 392
AluI AGCT 5 cut(s) 38, 95, 307, 362, 392
Alw21I GWGCWC 1 cut(s) 97
AlwI GGATC 1 cut(s) 215
Aor51HI AGCGCT 1 cut(s) 49
AoxI GGCC 2 cut(s) 27, 191
ApeKI GCWGC 2 cut(s) 131, 434
ApoI RAATTY 1 cut(s) 136
ArsI GACNNNNNNTTYG 2 cut(s) 102, 134
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 2 cut(s) 27, 371
AsuHPI GGTGA 5 cut(s) 243, 331, 361, 391, 421
AvaII GGWCC 1 cut(s) 371
BanII GRGCYC 2 cut(s) 8, 97
BbsI GAAGAC 3 cut(s) 307, 509, 651
Bbv12I GWGCWC 1 cut(s) 97
BbvI GCAGC 2 cut(s) 143, 446
BccI CCATC 1 cut(s) 553
BceAI ACGGC 1 cut(s) 475
BcgI CGANNNNNNTGC 2 cut(s) 120, 154
BclI TGATCA 1 cut(s) 567
BcuI ACTAGT 1 cut(s) 439
BfaI CTAG 4 cut(s) 204, 242, 260, 440
BfoI RGCGCY 1 cut(s) 51
BisI GCNGC 2 cut(s) 132, 435
BlsI GCNGC 2 cut(s) 133, 436
Bme18I GGWCC 1 cut(s) 371
BmgT120I GGNCC 2 cut(s) 27, 371
BmiI GGNNCC 1 cut(s) 462
BmrI ACTGGG 2 cut(s) 34, 466
BmsI GCATC 1 cut(s) 228
BmuI ACTGGG 2 cut(s) 34, 466
BpiI GAAGAC 3 cut(s) 307, 509, 651
BpmI CTGGAG 2 cut(s) 425, 620
BsaAI YACGTR 1 cut(s) 446
BsaWI WCCGGW 1 cut(s) 373
BsaXI ACNNNNNCTCC 2 cut(s) 333, 363
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 4 cut(s) 29, 221, 408, 461
BseGI GGATG 2 cut(s) 243, 559
BseMII CTCAG 2 cut(s) 354, 384
BseNI ACTGG 4 cut(s) 29, 221, 408, 461
BseRI GAGGAG 1 cut(s) 329
BseXI GCAGC 2 cut(s) 143, 446
BshFI GGCC 2 cut(s) 29, 193
BsiHKAI GWGCWC 1 cut(s) 97
BsiSI CCGG 2 cut(s) 194, 374
BsnI GGCC 2 cut(s) 29, 193
Bsp1286I GDGCHC 2 cut(s) 8, 97
Bsp143I GATC 5 cut(s) 141, 207, 248, 415, 567
BspANI GGCC 2 cut(s) 29, 193
BspCNI CTCAG 2 cut(s) 355, 385
BspLI GGNNCC 1 cut(s) 462
BspPI GGATC 1 cut(s) 215
BsrFI RCCGGY 1 cut(s) 193
BsrI ACTGG 4 cut(s) 29, 221, 408, 461
BssAI RCCGGY 1 cut(s) 193
BssMI GATC 5 cut(s) 141, 207, 248, 415, 567
Bst4CI ACNGT 1 cut(s) 637
Bst6I CTCTTC 1 cut(s) 306
BstBAI YACGTR 1 cut(s) 446
BstC8I GCNNGC 1 cut(s) 154
BstDEI CTNAG 3 cut(s) 363, 393, 419
BstF5I GGATG 2 cut(s) 243, 559
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 5 cut(s) 144, 210, 251, 418, 570
BstMBI GATC 5 cut(s) 141, 207, 248, 415, 567
BstMWI GCNNNNNNNGC 1 cut(s) 35
BstNSI RCATGY 1 cut(s) 332
BstSNI TACGTA 1 cut(s) 446
BstV1I GCAGC 2 cut(s) 143, 446
BstV2I GAAGAC 3 cut(s) 307, 509, 651
BstX2I RGATCY 1 cut(s) 207
BstYI RGATCY 1 cut(s) 207
BsuRI GGCC 2 cut(s) 29, 193
BtsCI GGATG 2 cut(s) 243, 559
Cac8I GCNNGC 1 cut(s) 154
CfoI GCGC 1 cut(s) 50
Cfr10I RCCGGY 1 cut(s) 193
Cfr13I GGNCC 2 cut(s) 27, 371
Csp6I GTAC 2 cut(s) 443, 447
CviAII CATG 1 cut(s) 329
CviJI RGCY 9 cut(s) 6, 29, 38, 95, 152, 193, 307, 362, 392
CviKI_1 RGCY 9 cut(s) 6, 29, 38, 95, 152, 193, 307, 362, 392
CviQI GTAC 2 cut(s) 443, 447
DdeI CTNAG 3 cut(s) 363, 393, 419
DpnI GATC 5 cut(s) 143, 209, 250, 417, 569
DpnII GATC 5 cut(s) 141, 207, 248, 415, 567
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
Ecl136II GAGCTC 1 cut(s) 95
Eco105I TACGTA 1 cut(s) 446
Eco24I GRGCYC 2 cut(s) 8, 97
Eco32I GATATC 1 cut(s) 548
Eco47I GGWCC 1 cut(s) 371
Eco47III AGCGCT 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 95
Eco57I CTGAAG 1 cut(s) 521
EcoICRI GAGCTC 1 cut(s) 95
EcoRV GATATC 1 cut(s) 548
EcoT38I GRGCYC 2 cut(s) 8, 97
FaeI CATG 1 cut(s) 332
FaiI YATR 7 cut(s) 17, 45, 60, 115, 330, 528, 618
FatI CATG 1 cut(s) 328
FbaI TGATCA 1 cut(s) 567
Fnu4HI GCNGC 2 cut(s) 132, 435
FokI GGATG 2 cut(s) 250, 566
FriOI GRGCYC 2 cut(s) 8, 97
Fsp4HI GCNGC 2 cut(s) 132, 435
FspBI CTAG 4 cut(s) 204, 242, 260, 440
GlaI GCGC 1 cut(s) 49
GluI GCNGC 2 cut(s) 132, 435
GsuI CTGGAG 2 cut(s) 425, 620
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 2 cut(s) 29, 193
HapII CCGG 2 cut(s) 194, 374
HhaI GCGC 1 cut(s) 50
Hin1II CATG 1 cut(s) 332
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HinfI GANTC 2 cut(s) 470, 522
HpaII CCGG 2 cut(s) 194, 374
HphI GGTGA 5 cut(s) 243, 331, 361, 391, 421
Hpy188I TCNGA 1 cut(s) 453
Hpy188III TCNNGA 2 cut(s) 551, 599
HpyCH4III ACNGT 1 cut(s) 637
HpyCH4IV ACGT 1 cut(s) 445
HpyCH4V TGCA 1 cut(s) 272
HpyF10VI GCNNNNNNNGC 1 cut(s) 35
HpyF3I CTNAG 3 cut(s) 363, 393, 419
HpySE526I ACGT 1 cut(s) 445
Hsp92II CATG 1 cut(s) 332
HspAI GCGC 1 cut(s) 48
Ksp22I TGATCA 1 cut(s) 567
Kzo9I GATC 5 cut(s) 141, 207, 248, 415, 567
LmnI GCTCC 2 cut(s) 92, 341
Lsp1109I GCAGC 2 cut(s) 143, 446
LweI GCATC 1 cut(s) 228
MaeI CTAG 4 cut(s) 204, 242, 260, 440
MaeII ACGT 1 cut(s) 445
MaeIII GTNAC 1 cut(s) 492
MalI GATC 5 cut(s) 143, 209, 250, 417, 569
MboI GATC 5 cut(s) 141, 207, 248, 415, 567
MboII GAAGA 5 cut(s) 312, 323, 514, 616, 656
MflI RGATCY 1 cut(s) 207
MhlI GDGCHC 2 cut(s) 8, 97
MluCI AATT 2 cut(s) 136, 185
MnlI CCTC 9 cut(s) 101, 174, 183, 228, 307, 310, 340, 370, 400
MseI TTAA 3 cut(s) 230, 516, 581
MspA1I CMGCKG 1 cut(s) 307
MspI CCGG 2 cut(s) 194, 374
MwoI GCNNNNNNNGC 1 cut(s) 35
NdeII GATC 5 cut(s) 141, 207, 248, 415, 567
NlaIII CATG 1 cut(s) 332
NlaIV GGNNCC 1 cut(s) 462
NmuCI GTSAC 1 cut(s) 492
NspI RCATGY 1 cut(s) 332
PfeI GAWTC 2 cut(s) 470, 522
PkrI GCNGC 2 cut(s) 133, 436
Ppu21I YACGTR 1 cut(s) 446
Psp124BI GAGCTC 1 cut(s) 97
PspN4I GGNNCC 1 cut(s) 462
PspPI GGNCC 2 cut(s) 27, 371
PsuI RGATCY 1 cut(s) 207
PvuII CAGCTG 1 cut(s) 307
RsaI GTAC 2 cut(s) 444, 448
RsaNI GTAC 2 cut(s) 443, 447
SacI GAGCTC 1 cut(s) 97
SaqAI TTAA 3 cut(s) 230, 516, 581
SatI GCNGC 2 cut(s) 132, 435
Sau3AI GATC 5 cut(s) 141, 207, 248, 415, 567
Sau96I GGNCC 2 cut(s) 27, 371
SduI GDGCHC 2 cut(s) 8, 97
SfaNI GCATC 1 cut(s) 228
SinI GGWCC 1 cut(s) 371
SnaBI TACGTA 1 cut(s) 446
SpeI ACTAGT 1 cut(s) 439
Sse9I AATT 2 cut(s) 136, 185
SspMI CTAG 4 cut(s) 204, 242, 260, 440
SstI GAGCTC 1 cut(s) 97
TaaI ACNGT 1 cut(s) 637
TaiI ACGT 1 cut(s) 448
TaqI TCGA 2 cut(s) 140, 298
TasI AATT 2 cut(s) 136, 185
TfiI GAWTC 2 cut(s) 470, 522
Tru1I TTAA 3 cut(s) 230, 516, 581
Tru9I TTAA 3 cut(s) 230, 516, 581
TseFI GTSAC 1 cut(s) 492
TseI GCWGC 2 cut(s) 131, 434
Tsp45I GTSAC 1 cut(s) 492
TspDTI ATGAA 2 cut(s) 188, 657
TspGWI ACGGA 1 cut(s) 534
VpaK11BI GGWCC 1 cut(s) 371
XapI RAATTY 1 cut(s) 136
XceI RCATGY 1 cut(s) 332
XspI CTAG 4 cut(s) 204, 242, 260, 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.