Rh5DG081700

Lysyl-tRNA synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018406
Physical Location & Seq
Reverse (-)
7155672 .. 7159350
3679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG081700.1

Sequence Viewer

Length: 1860 bp
ATGGCGGGAGAGGAATCCACGCCGTACTTGGAAGAGCTCCGCCATCTCCGGAGCATGGCCAAGCACCCCAGCATCATCAACCTCCTTTCCTCTGAGATTTCCAGTTTGGAAAAGGATCAATCTCCACCACTACCGCCGCAATCTTCACCACCGCGCCTCAGCCAAGAGGTCACGTCAGCAGCTGAGAAGGCAAAGCAGACTAATGTCACGGCAAGATCATGCGGCAAGAAATATGTAGCAAGTGAAGATGAAAAGGATCCGACGAAATACTACGAAAACAGGCTGAATTACATTGCTACCCTGAAAAATCCATATCCCCATGAATCCTCTGTTTCAAAGAGTATAACTGAATATGTGAAGGAGTATGAAGGCCTCCTCAAGGACCAACAAGGGGTGCATCTTGAGAATGTCACAGAATCCTTGGCTGGAATGGTCATGAAACAAAGATCCTCCGGCCCAGGGTGTTTCTTTTATGATTTATGTGGTGATCGAGCTCGAGTACAAATTTATGCTAGTGCCATCAATTCGGATATGGATAAATCTGAATTCGTTAAGATCCACTCTAATGTGAAGCGCGGTGACATTGTTAATGTGACCGGGTTTCCAGGTAAAACAATGAGTGGCCAGATCAGCATTTTTGCAAGATCATTTGTAGTCTTAGCACCTTGTCTCCACATGATGCCTACTGTCAATCCAAAGAAAATAACTGAGGAAACTGAAGAATGGCATCCGGGACGTCCCAGGAATCCTGAAACATACAGTTTAAAAGACCAGGAAACTCGCTATCGTCAGCGTCACCTGGATTTAAGGATAAACCCTGATGTTCAACGTATAGTGGTGGAGACCAGGAGTGAGATCACTTTCTATATTAGGAGGTTTCTTCATAGTCGTCAGTTCCGTGAAGTTGAAACTCCAATATTGAACACGACAGCTGGTGGAGCAACTGCGCGTCCTTTTATGACGCATCACAATGAACTTGACATGGAACTTTACATGCGCATTGCACCCGAACTCTATCTTAAACAGCTAGTTGTTGGTGGAATTGAACGCGTTTTTGAGATAGGCAAACAGTTTAGGAATGAGGGTATTGATCTGACACATAATCCTGAGTTCGCTACCTGTGAATTCTATCAGGCTTATGCAGACTATAATCACATGATGGAGCTCACTGAGGAAATGTTGAGTGGAATGGTCAAGGAACTTACAGGTGGATATAAGATAATGTATCATTCAAATGGACTTGATAAGGATCCAATTGAGATAGACTTCACACCCCCTTTCAGACGGATTGACATGATTGAGGAATTAAATAAGATTGCAAATCTTGAAATAAATCCGGAGGATCTCTTCAGTGAGGAAGCTAACAGTGATCTGAAGGTCGCGTGCGAGAAGTATGGGATCCAATGTCCACCTCCTGAAACAACAACTCGTTTATTGGACAAGCTTGTTGATCACTTTTTGGAAGAGCAATGTGTGAATCCAACATTTATAATCAACCATCCCGAGATAATGAGTCCATTGGCAAAGTGGCATCGATCAAGACCTGGCTTGACAGAACGCTTTGAATTGTTTGTCAGCAAGCATGAGCTTTGTAATGGATACACTGAACTGAATAATCCTATTGTCCAACGCCAACGATTCGCTAATCAACTCAAGGATAGACAATCCGGTGATGATGAAGCAATGCCTTTGGATGAAGGGTTTTGTAGAGCTCTTGAATATGGATTACCTCCAACAGGTGGTGTGGGAATAGGCATTGACCGCCTTTGTATGCTTTTAACTGATTCTGTGAACATTAAGGAAGTCATCCCCTTCCCAACCATGAAGCCACAGGATCAGCCTTCAGCTAGACCCAGCTAG

Protein Analysis

619

Amino Acids

70.67

Weight (kDa)

5.57

Isoelectric Point (pI)

56.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_anti-codon PF01336 140 - 220 2.9e-08 OB-fold nucleic acid binding domain
tRNA-synt_2 PF00152 257 - 608 2e-90 tRNA synthetases class II (D, K and N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018406)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g37790
rosa_chinensis RchiOBHm_Chr5g0011081
rosa_laevigata RLG00000031808
rosa_multiflora Rmu_sc0003242.1_g000009
rosa_roxburghii Rroxscaffold_1G00065330
rosa_rugosa Rorug04G0455100 Rorug04G0455100
rosa_samantha Rh5DG081700
rosa_wichuraiana Rw0G000170 Rw0G017780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1490
AatII GACGTC 1 cut(s) 739
Acc16I TGCGCA 1 cut(s) 998
AccB7I CCANNNNNTGG 1 cut(s) 1739
AccII CGCG 5 cut(s) 154, 576, 949, 1050, 1382
AccIII TCCGGA 2 cut(s) 48, 1336
AciI CCGC 8 cut(s) 5, 40, 134, 137, 152, 222, 576, 1762
AcoI YGGCCR 2 cut(s) 57, 622
AcsI RAATTY 3 cut(s) 504, 545, 1124
AcuI CTGAAG 4 cut(s) 738, 1333, 1394, 1827
AcyI GRCGYC 1 cut(s) 736
AfaI GTAC 2 cut(s) 26, 501
AfiI CCNNNNNNNGG 6 cut(s) 55, 379, 391, 459, 1736, 1739
AflIII ACRYGT 1 cut(s) 1048
AgsI TTSAA 9 cut(s) 336, 827, 908, 922, 1046, 1233, 1328, 1565, 1718
AjiI CACGTC 1 cut(s) 174
AjnI CCWGG 7 cut(s) 457, 604, 740, 771, 798, 845, 1543
Alw21I GWGCWC 4 cut(s) 39, 496, 1167, 1714
Alw26I GTCTC 2 cut(s) 674, 836
AlwNI CAGNNNCTG 1 cut(s) 182
Ama87I CYCGRG 2 cut(s) 495, 1502
Aor13HI TCCGGA 2 cut(s) 48, 1336
AoxI GGCC 4 cut(s) 57, 370, 454, 622
ApeKI GCWGC 1 cut(s) 179
ApoI RAATTY 3 cut(s) 504, 545, 1124
AspLEI GCGC 4 cut(s) 156, 576, 949, 999
AspS9I GGNCC 2 cut(s) 382, 455
AsuC2I CCSGG 2 cut(s) 598, 732
AsuHPI GGTGA 5 cut(s) 138, 497, 590, 788, 1682
AvaI CYCGRG 2 cut(s) 495, 1502
AvaII GGWCC 1 cut(s) 382
BalI TGGCCA 2 cut(s) 59, 624
BamHI GGATCC 3 cut(s) 256, 1249, 1398
BanII GRGCYC 4 cut(s) 39, 496, 1167, 1714
Bbv12I GWGCWC 4 cut(s) 39, 496, 1167, 1714
BbvCI CCTCAGC 1 cut(s) 158
BbvI GCAGC 1 cut(s) 191
BccI CCATC 4 cut(s) 51, 527, 1153, 1506
BceAI ACGGC 2 cut(s) 7, 225
BciT130I CCWGG 7 cut(s) 459, 606, 742, 773, 800, 847, 1545
BciVI GTATCC 1 cut(s) 1592
BclI TGATCA 1 cut(s) 1450
BcnI CCSGG 2 cut(s) 598, 732
BcoDI GTCTC 2 cut(s) 674, 836
BfaI CTAG 4 cut(s) 513, 1028, 1848, 1858
BfuI GTATCC 1 cut(s) 1592
BisI GCNGC 3 cut(s) 137, 180, 223
BlsI GCNGC 3 cut(s) 138, 181, 224
Bme1390I CCNGG 9 cut(s) 459, 598, 606, 732, 742, 773, 800, 847, 1545
Bme18I GGWCC 1 cut(s) 382
BmeT110I CYCGRG 2 cut(s) 495, 1502
BmgBI CACGTC 1 cut(s) 174
BmgT120I GGNCC 2 cut(s) 382, 455
BmiI GGNNCC 3 cut(s) 258, 1251, 1400
BmrFI CCNGG 9 cut(s) 459, 598, 606, 732, 742, 773, 800, 847, 1545
BmsI GCATC 6 cut(s) 81, 406, 669, 736, 973, 1540
BoxI GACNNNNGTC 1 cut(s) 203
Bpu10I CCTNAGC 1 cut(s) 158
BpuEI CTTGAG 3 cut(s) 362, 422, 1637
BpuMI CCSGG 2 cut(s) 598, 732
Bsa29I ATCGAT 1 cut(s) 1534
BsaBI GATNNNNATC 1 cut(s) 1248
BsaHI GRCGYC 1 cut(s) 736
BsaI GGTCTC 1 cut(s) 836
BsaJI CCNNGG 4 cut(s) 420, 457, 458, 740
BsaWI WCCGGW 3 cut(s) 48, 1336, 1667
BsaXI ACNNNNNCTCC 2 cut(s) 654, 684
Bsc4I CCNNNNNNNGG 6 cut(s) 55, 379, 391, 459, 1736, 1739
Bse1I ACTGG 1 cut(s) 102
Bse3DI GCAATG 4 cut(s) 291, 999, 1475, 1689
Bse8I GATNNNNATC 1 cut(s) 1248
BseAI TCCGGA 2 cut(s) 48, 1336
BseBI CCWGG 7 cut(s) 459, 606, 742, 773, 800, 847, 1545
BseCI ATCGAT 1 cut(s) 1534
BseDI CCNNGG 4 cut(s) 420, 457, 458, 740
BseGI GGATG 4 cut(s) 727, 1498, 1699, 1806
BseJI GATNNNNATC 1 cut(s) 1248
BseLI CCNNNNNNNGG 6 cut(s) 55, 379, 391, 459, 1736, 1739
BseMI GCAATG 4 cut(s) 291, 999, 1475, 1689
BseMII CTCAG 6 cut(s) 84, 172, 174, 699, 1098, 1161
BseNI ACTGG 1 cut(s) 102
BseRI GAGGAG 1 cut(s) 365
BseXI GCAGC 1 cut(s) 191
BseYI CCCAGC 2 cut(s) 68, 1853
Bsh1236I CGCG 5 cut(s) 154, 576, 949, 1050, 1382
BshFI GGCC 4 cut(s) 59, 372, 456, 624
BshVI ATCGAT 1 cut(s) 1534
BsiHKAI GWGCWC 4 cut(s) 39, 496, 1167, 1714
BsiHKCI CYCGRG 2 cut(s) 495, 1502
BsiSI CCGG 6 cut(s) 49, 453, 597, 731, 1337, 1668
BslFI GGGAC 2 cut(s) 723, 747
BslI CCNNNNNNNGG 6 cut(s) 55, 379, 391, 459, 1736, 1739
BsmAI GTCTC 2 cut(s) 674, 836
BsmFI GGGAC 2 cut(s) 723, 747
BsnI GGCC 4 cut(s) 59, 372, 456, 624
Bso31I GGTCTC 1 cut(s) 836
BsoBI CYCGRG 2 cut(s) 495, 1502
Bsp1286I GDGCHC 4 cut(s) 39, 496, 1167, 1714
Bsp13I TCCGGA 2 cut(s) 48, 1336
BspACI CCGC 8 cut(s) 5, 40, 134, 137, 152, 222, 576, 1762
BspANI GGCC 4 cut(s) 59, 372, 456, 624
BspCNI CTCAG 6 cut(s) 85, 171, 175, 700, 1099, 1162
BspDI ATCGAT 1 cut(s) 1534
BspEI TCCGGA 2 cut(s) 48, 1336
BspFNI CGCG 5 cut(s) 154, 576, 949, 1050, 1382
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 3 cut(s) 258, 1251, 1400
BspQI GCTCTTC 2 cut(s) 27, 1458
BspTNI GGTCTC 1 cut(s) 836
BsrDI GCAATG 4 cut(s) 291, 999, 1475, 1689
BsrI ACTGG 1 cut(s) 102
BssECI CCNNGG 4 cut(s) 420, 457, 458, 740
BssNI GRCGYC 1 cut(s) 736
BssT1I CCWWGG 1 cut(s) 420
Bst2UI CCWGG 7 cut(s) 459, 606, 742, 773, 800, 847, 1545
Bst4CI ACNGT 4 cut(s) 688, 761, 1071, 1367
Bst6I CTCTTC 3 cut(s) 27, 1352, 1458
BstACI GRCGYC 1 cut(s) 736
BstC8I GCNNGC 2 cut(s) 1384, 1580
BstDEI CTNAG 7 cut(s) 93, 158, 183, 658, 708, 1107, 1170
BstENI CCTNNNNNAGG 2 cut(s) 377, 1734
BstF5I GGATG 4 cut(s) 727, 1498, 1699, 1806
BstFNI CGCG 5 cut(s) 154, 576, 949, 1050, 1382
BstHHI GCGC 4 cut(s) 156, 576, 949, 999
BstMAI GTCTC 2 cut(s) 674, 836
BstMWI GCNNNNNNNGC 4 cut(s) 188, 630, 938, 1761
BstNI CCWGG 7 cut(s) 459, 606, 742, 773, 800, 847, 1545
BstNSI RCATGY 1 cut(s) 997
BstPAI GACNNNNGTC 1 cut(s) 203
BstSCI CCNGG 9 cut(s) 457, 596, 604, 730, 740, 771, 798, 845, 1543
BstUI CGCG 5 cut(s) 154, 576, 949, 1050, 1382
BstV1I GCAGC 1 cut(s) 191
BstX2I RGATCY 6 cut(s) 256, 446, 555, 1249, 1342, 1398
BstYI RGATCY 6 cut(s) 256, 446, 555, 1249, 1342, 1398
Bsu15I ATCGAT 1 cut(s) 1534
BsuI GTATCC 1 cut(s) 1592
BsuRI GGCC 4 cut(s) 59, 372, 456, 624
BsuTUI ATCGAT 1 cut(s) 1534
BtrI CACGTC 1 cut(s) 174
BtsCI GGATG 4 cut(s) 727, 1498, 1699, 1806
BtsIMutI CAGTG 4 cut(s) 1167, 1357, 1372, 1602
Cac8I GCNNGC 2 cut(s) 1384, 1580
CaiI CAGNNNCTG 1 cut(s) 182
CciI TCATGA 1 cut(s) 435
CfoI GCGC 4 cut(s) 156, 576, 949, 999
Cfr13I GGNCC 2 cut(s) 382, 455
ClaI ATCGAT 1 cut(s) 1534
CseI GACGC 3 cut(s) 782, 938, 970
Csp6I GTAC 2 cut(s) 25, 500
CviQI GTAC 2 cut(s) 25, 500
DdeI CTNAG 7 cut(s) 93, 158, 183, 658, 708, 1107, 1170
DraI TTTAAA 1 cut(s) 765
EaeI YGGCCR 2 cut(s) 57, 622
Eam1104I CTCTTC 3 cut(s) 27, 1352, 1458
EarI CTCTTC 3 cut(s) 27, 1352, 1458
EciI GGCGGA 1 cut(s) 29
Ecl136II GAGCTC 4 cut(s) 37, 494, 1165, 1712
Eco130I CCWWGG 1 cut(s) 420
Eco147I AGGCCT 1 cut(s) 372
Eco24I GRGCYC 4 cut(s) 39, 496, 1167, 1714
Eco31I GGTCTC 1 cut(s) 836
Eco47I GGWCC 1 cut(s) 382
Eco53kI GAGCTC 4 cut(s) 37, 494, 1165, 1712
Eco57I CTGAAG 4 cut(s) 738, 1333, 1394, 1827
Eco88I CYCGRG 2 cut(s) 495, 1502
EcoICRI GAGCTC 4 cut(s) 37, 494, 1165, 1712
EcoNI CCTNNNNNAGG 2 cut(s) 377, 1734
EcoRI GAATTC 2 cut(s) 545, 1124
EcoRII CCWGG 7 cut(s) 457, 604, 740, 771, 798, 845, 1543
EcoT14I CCWWGG 1 cut(s) 420
EcoT38I GRGCYC 4 cut(s) 39, 496, 1167, 1714
ErhI CCWWGG 1 cut(s) 420
FaqI GGGAC 2 cut(s) 723, 747
FbaI TGATCA 1 cut(s) 1450
Fnu4HI GCNGC 3 cut(s) 137, 180, 223
FokI GGATG 4 cut(s) 714, 1485, 1706, 1793
FriOI GRGCYC 4 cut(s) 39, 496, 1167, 1714
Fsp4HI GCNGC 3 cut(s) 137, 180, 223
FspAI RTGCGCAY 1 cut(s) 998
FspBI CTAG 4 cut(s) 513, 1028, 1848, 1858
FspI TGCGCA 1 cut(s) 998
GlaI GCGC 4 cut(s) 155, 575, 948, 998
GluI GCNGC 3 cut(s) 137, 180, 223
GsaI CCCAGC 2 cut(s) 72, 1857
HaeIII GGCC 4 cut(s) 59, 372, 456, 624
HapII CCGG 6 cut(s) 49, 453, 597, 731, 1337, 1668
HgaI GACGC 3 cut(s) 782, 938, 970
HhaI GCGC 4 cut(s) 156, 576, 949, 999
Hin1I GRCGYC 1 cut(s) 736
Hin6I GCGC 4 cut(s) 154, 574, 947, 997
HinP1I GCGC 4 cut(s) 154, 574, 947, 997
HindIII AAGCTT 1 cut(s) 1442
HinfI GANTC 8 cut(s) 14, 323, 416, 745, 1477, 1513, 1638, 1784
HpaII CCGG 6 cut(s) 49, 453, 597, 731, 1337, 1668
HphI GGTGA 5 cut(s) 138, 497, 590, 788, 1682
Hpy166II GTNNAC 2 cut(s) 1409, 1792
Hpy188I TCNGA 7 cut(s) 94, 261, 529, 544, 1095, 1283, 1374
Hpy8I GTNNAC 2 cut(s) 1409, 1792
Hpy99I CGWCG 1 cut(s) 265
HpyAV CCTTC 7 cut(s) 181, 352, 362, 1369, 1691, 1822, 1851
HpyCH4III ACNGT 4 cut(s) 688, 761, 1071, 1367
HpyCH4IV ACGT 3 cut(s) 173, 736, 829
HpyCH4V TGCA 5 cut(s) 397, 641, 1004, 1142, 1319
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 630, 938, 1761
HpyF3I CTNAG 7 cut(s) 93, 158, 183, 658, 708, 1107, 1170
HpySE526I ACGT 3 cut(s) 173, 736, 829
Hsp92I GRCGYC 1 cut(s) 736
HspAI GCGC 4 cut(s) 154, 574, 947, 997
Kpn2I TCCGGA 2 cut(s) 48, 1336
Ksp22I TGATCA 1 cut(s) 1450
LguI GCTCTTC 2 cut(s) 27, 1458
LmnI GCTCC 4 cut(s) 42, 51, 938, 1162
Lsp1109I GCAGC 1 cut(s) 191
LweI GCATC 6 cut(s) 81, 406, 669, 736, 973, 1540
MaeI CTAG 4 cut(s) 513, 1028, 1848, 1858
MaeII ACGT 3 cut(s) 173, 736, 829
MaeIII GTNAC 6 cut(s) 169, 205, 409, 578, 592, 794
MboII GAAGA 7 cut(s) 44, 135, 257, 731, 872, 1339, 1475
MfeI CAATTG 1 cut(s) 1254
MflI RGATCY 6 cut(s) 256, 446, 555, 1249, 1342, 1398
MhlI GDGCHC 4 cut(s) 39, 496, 1167, 1714
MlsI TGGCCA 2 cut(s) 59, 624
MluCI AATT 9 cut(s) 286, 504, 523, 545, 1041, 1124, 1254, 1304, 1565
MluI ACGCGT 1 cut(s) 1048
MluNI TGGCCA 2 cut(s) 59, 624
MlyI GAGTC 1 cut(s) 1522
MmeI TCCRAC 4 cut(s) 284, 1505, 1651, 1757
Mox20I TGGCCA 2 cut(s) 59, 624
MroI TCCGGA 2 cut(s) 48, 1336
MscI TGGCCA 2 cut(s) 59, 624
MseI TTAA 8 cut(s) 552, 588, 764, 806, 1020, 1307, 1778, 1797
MslI CAYNNNNRTG 3 cut(s) 564, 969, 1233
Msp20I TGGCCA 2 cut(s) 59, 624
MspA1I CMGCKG 2 cut(s) 182, 932
MspI CCGG 6 cut(s) 49, 453, 597, 731, 1337, 1668
MspR9I CCNGG 9 cut(s) 459, 598, 606, 732, 742, 773, 800, 847, 1545
MunI CAATTG 1 cut(s) 1254
MvaI CCWGG 7 cut(s) 459, 606, 742, 773, 800, 847, 1545
MvnI CGCG 5 cut(s) 154, 576, 949, 1050, 1382
MwoI GCNNNNNNNGC 4 cut(s) 188, 630, 938, 1761
NciI CCSGG 2 cut(s) 598, 732
NlaIV GGNNCC 3 cut(s) 258, 1251, 1400
NmuCI GTSAC 6 cut(s) 169, 205, 409, 578, 592, 794
NsbI TGCGCA 1 cut(s) 998
NspI RCATGY 1 cut(s) 997
PaeR7I CTCGAG 1 cut(s) 495
PagI TCATGA 1 cut(s) 435
PasI CCCWGGG 1 cut(s) 458
PceI AGGCCT 1 cut(s) 372
PciSI GCTCTTC 2 cut(s) 27, 1458
PcsI WCGNNNNNNNCGW 1 cut(s) 895
PfeI GAWTC 7 cut(s) 14, 323, 416, 745, 1477, 1638, 1784
PflMI CCANNNNNTGG 1 cut(s) 1739
PfoI TCCNGGA 1 cut(s) 730
PkrI GCNGC 3 cut(s) 138, 181, 224
PleI GAGTC 1 cut(s) 1521
PpsI GAGTC 1 cut(s) 1521
PshAI GACNNNNGTC 1 cut(s) 203
PsiI TTATAA 1 cut(s) 1490
Psp124BI GAGCTC 4 cut(s) 39, 496, 1167, 1714
Psp6I CCWGG 7 cut(s) 457, 604, 740, 771, 798, 845, 1543
PspFI CCCAGC 2 cut(s) 68, 1853
PspGI CCWGG 7 cut(s) 457, 604, 740, 771, 798, 845, 1543
PspN4I GGNNCC 3 cut(s) 258, 1251, 1400
PspPI GGNCC 2 cut(s) 382, 455
PspXI VCTCGAGB 1 cut(s) 495
PstNI CAGNNNCTG 1 cut(s) 182
PsuI RGATCY 6 cut(s) 256, 446, 555, 1249, 1342, 1398
PvuII CAGCTG 2 cut(s) 182, 932
RsaI GTAC 2 cut(s) 26, 501
RsaNI GTAC 2 cut(s) 25, 500
RseI CAYNNNNRTG 3 cut(s) 564, 969, 1233
SacI GAGCTC 4 cut(s) 39, 496, 1167, 1714
SapI GCTCTTC 2 cut(s) 27, 1458
SaqAI TTAA 8 cut(s) 552, 588, 764, 806, 1020, 1307, 1778, 1797
SatI GCNGC 3 cut(s) 137, 180, 223
Sau96I GGNCC 2 cut(s) 382, 455
SchI GAGTC 1 cut(s) 1522
ScrFI CCNGG 9 cut(s) 459, 598, 606, 732, 742, 773, 800, 847, 1545
SduI GDGCHC 4 cut(s) 39, 496, 1167, 1714
SfaNI GCATC 6 cut(s) 81, 406, 669, 736, 973, 1540
Sfr274I CTCGAG 1 cut(s) 495
SinI GGWCC 1 cut(s) 382
SlaI CTCGAG 1 cut(s) 495
SmiMI CAYNNNNRTG 3 cut(s) 564, 969, 1233
SmlI CTYRAG 4 cut(s) 377, 401, 495, 1652
SmoI CTYRAG 4 cut(s) 377, 401, 495, 1652
Sse9I AATT 9 cut(s) 286, 504, 523, 545, 1041, 1124, 1254, 1304, 1565
SseBI AGGCCT 1 cut(s) 372
SsiI CCGC 8 cut(s) 5, 40, 134, 137, 152, 222, 576, 1762
SspI AATATT 1 cut(s) 918
SspMI CTAG 4 cut(s) 513, 1028, 1848, 1858
SstI GAGCTC 4 cut(s) 39, 496, 1167, 1714
StuI AGGCCT 1 cut(s) 372
StyD4I CCNGG 9 cut(s) 457, 596, 604, 730, 740, 771, 798, 845, 1543
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 4 cut(s) 688, 761, 1071, 1367
TaiI ACGT 3 cut(s) 176, 739, 832
TaqI TCGA 3 cut(s) 490, 496, 1534
TasI AATT 9 cut(s) 286, 504, 523, 545, 1041, 1124, 1254, 1304, 1565
TatI WGTACW 1 cut(s) 499
TauI GCSGC 2 cut(s) 139, 225
TfiI GAWTC 7 cut(s) 14, 323, 416, 745, 1477, 1638, 1784
Tru1I TTAA 8 cut(s) 552, 588, 764, 806, 1020, 1307, 1778, 1797
Tru9I TTAA 8 cut(s) 552, 588, 764, 806, 1020, 1307, 1778, 1797
TscAI CASTG 4 cut(s) 1174, 1357, 1372, 1609
TseFI GTSAC 6 cut(s) 169, 205, 409, 578, 592, 794
TseI GCWGC 1 cut(s) 179
Tsp45I GTSAC 6 cut(s) 169, 205, 409, 578, 592, 794
TspDTI ATGAA 9 cut(s) 264, 336, 381, 452, 872, 987, 1692, 1710, 1838
TspGWI ACGGA 2 cut(s) 887, 1300
TspRI CASTG 4 cut(s) 1174, 1357, 1372, 1609
Van91I CCANNNNNTGG 1 cut(s) 1739
VpaK11BI GGWCC 1 cut(s) 382
XagI CCTNNNNNAGG 2 cut(s) 377, 1734
XapI RAATTY 3 cut(s) 504, 545, 1124
XceI RCATGY 1 cut(s) 997
XcmI CCANNNNNNNNNTGG 2 cut(s) 25, 1524
XhoI CTCGAG 1 cut(s) 495
XspI CTAG 4 cut(s) 513, 1028, 1848, 1858
ZraI GACGTC 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.