Rw0G017780

Lysyl-tRNA synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00792
OG0018406
Physical Location & Seq
Forward (+)
27267 .. 30946
3680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G017780.1

Sequence Viewer

Length: 1782 bp
ATGGCGGGAGAGGAATCCACGCCGTACTTGGAAGAGCTCCGCCATCTCCGGAGCATGGCCAAGCACCCCAGCATCATCAACCTCCTTTCCTCTGAGATTTCCAGTTTGGAAAAGGATCAAACTCCACCACTACCGCCGCAATCTTCACCACCGCGCCTCAGCCAAGAGGTCACGTCAGCAGCTGAGAAGGCAAAGCAGAAATACTATGAAAACAGGCTGAATTACATTGCTACCCTGAAAAATCCATATCCCCATGAATCCTCTGTTTCAAAGAGTATAACTGAATATGTGAAGGAGTATGGAGGCCTCCTCAAGCACCAACAAGGGGTGCATCTTGAGAACGTCACAGAATCCTTGGCTGGAATGGTCATGAAACAAAGATCCTCCGGCCCAGGGTGTTTCTTTTATGATTTATGTGGTGATCGAGCTCGGGTACAAATTTATGCTAGTGCCAGCAATTCGGATATGGATAAATCTGAATTCGTTAAGATCCACTCTAATGTGAAGCGCGGTGACATTGTTAATGTGACCGGGTTTCCAGGTAAAACAATGAGTGGCCAGATCAGCATTTTTGCAAGATCATTTGTAGTCTTAGCACCTTGTCTCCACATGATGCCTACTGTCAATCCAAAGGAAACTGAAGAATGGCATCCGGGACGTCCCAGGAATCCTGAAACATACAGTTTAAAAGACCAGGAAACTCGCTATCGTCAGCGTCACCTGGATTTAAGGATAAACCCTGATGTTCAACGTATAGTGGTGGAGACCAGGAGTGAGATCACTTTCTATATTAGGAGGTTTCTTCATAGTCGTCAGTTCCGTGAAGTTGAAACTCCAATATTGAACACGACAGCTGGTGGAGCAACTGCGCGTCCTTTTATGACGCATCACAATGAACTTGACATGGAACTTTACATGCGCATTGCACCCGAACTCTATCTTAAACAGCTAGTTGTTGGTGGAATTGAACGCGTTTTTGAGATAGGCAAACAGTTTAGGAACGAGGGTATTGATCTGACACATAATCCTGAGTTCGCTACCTGTGAATTCTATCAGGCTTATGCAGACTATAATCACATGATGGAGCTCACTGAGGAAATGTTGAGTGGAATGGTCAAGGAACTTACAGGTGGATATAAGATAATGTATCATTCAAATGGACTTGATAAGGATCCAATTGAGATAGACTTCACTCCCCCTTTCAGACGGATTGACATGATTGAGGAATTAAATAAGATTGCAAATCTTGAAATAAATCCGGAGGATCTCTTCAGTGAGGAAGCTAACAGTGATCTGAAGGTCGCGTGCGAGAAGTATGGGATCCAATGTCCACCTCCTGAAACAACAACTCGTTTATTGGACAAGCTTGTTGATCACTTTTTGGAAGAGCAATGTGTGAATCCAACATTTATAATCAACCATCCCGAGATAATGAGTCCATTGGCAAAGTGGCATCGATCAAGACCTGGCTTGACAGAACGCTTTGAATTGTTTGTCAGCAAGCATGAGCTTTGTAATGGATACACTGAACTGAATAATCCTATTGTCCAACGCCAACGATTCGCTAATCAACTCAAGGATAGACAATCCGGTGATGATGAAGCAATGCCTTTGGATGAAGGGTTTTGTAGAGCTCTTGAATATGGATTACCTCCAACAGGTGGTGTGGGAATAGGCATTGACCGCCTTTGTATGCTTTTAACTGATTCTGTGAACATTAAGGAAGTCATCCCCTTCCCAACCATGAAGCCACAGGATCAGCCTTCAGCTAGACCCAGCTAG

Protein Analysis

593

Amino Acids

67.75

Weight (kDa)

5.7

Isoelectric Point (pI)

55.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_anti-codon PF01336 118 - 198 2.7e-08 OB-fold nucleic acid binding domain
tRNA-synt_2 PF00152 231 - 582 1.8e-90 tRNA synthetases class II (D, K and N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018406)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g37790
rosa_chinensis RchiOBHm_Chr5g0011081
rosa_laevigata RLG00000031808
rosa_multiflora Rmu_sc0003242.1_g000009
rosa_roxburghii Rroxscaffold_1G00065330
rosa_rugosa Rorug04G0455100 Rorug04G0455100
rosa_samantha Rh5DG081700
rosa_wichuraiana Rw0G000170 Rw0G017780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1412
AatII GACGTC 1 cut(s) 661
Acc16I TGCGCA 1 cut(s) 920
AccB7I CCANNNNNTGG 1 cut(s) 1661
AccII CGCG 5 cut(s) 154, 510, 871, 972, 1304
AccIII TCCGGA 2 cut(s) 48, 1258
AciI CCGC 7 cut(s) 5, 40, 134, 137, 152, 510, 1684
AclWI GGATC 9 cut(s) 123, 375, 484, 1166, 1179, 1272, 1315, 1328, 1764
AcoI YGGCCR 2 cut(s) 57, 556
AcsI RAATTY 3 cut(s) 438, 479, 1046
AcuI CTGAAG 4 cut(s) 660, 1255, 1316, 1749
AcyI GRCGYC 1 cut(s) 658
AfaI GTAC 2 cut(s) 26, 435
AfiI CCNNNNNNNGG 5 cut(s) 55, 325, 393, 1658, 1661
AflIII ACRYGT 1 cut(s) 970
AgsI TTSAA 9 cut(s) 270, 749, 830, 844, 968, 1155, 1250, 1487, 1640
AjiI CACGTC 1 cut(s) 174
AjnI CCWGG 7 cut(s) 391, 538, 662, 693, 720, 767, 1465
Alw21I GWGCWC 4 cut(s) 39, 430, 1089, 1636
Alw26I GTCTC 2 cut(s) 608, 758
AlwI GGATC 9 cut(s) 123, 375, 484, 1166, 1179, 1272, 1315, 1328, 1764
AlwNI CAGNNNCTG 1 cut(s) 182
Ama87I CYCGRG 2 cut(s) 429, 1424
Aor13HI TCCGGA 2 cut(s) 48, 1258
AoxI GGCC 4 cut(s) 57, 304, 388, 556
ApeKI GCWGC 1 cut(s) 179
ApoI RAATTY 3 cut(s) 438, 479, 1046
AspLEI GCGC 4 cut(s) 156, 510, 871, 921
AspS9I GGNCC 1 cut(s) 389
AsuC2I CCSGG 2 cut(s) 532, 654
AsuHPI GGTGA 5 cut(s) 138, 431, 524, 710, 1604
AvaI CYCGRG 2 cut(s) 429, 1424
BalI TGGCCA 2 cut(s) 59, 558
BamHI GGATCC 2 cut(s) 1171, 1320
BanII GRGCYC 4 cut(s) 39, 430, 1089, 1636
Bbv12I GWGCWC 4 cut(s) 39, 430, 1089, 1636
BbvCI CCTCAGC 1 cut(s) 158
BbvI GCAGC 1 cut(s) 191
BccI CCATC 3 cut(s) 51, 1075, 1428
BceAI ACGGC 1 cut(s) 7
BciT130I CCWGG 7 cut(s) 393, 540, 664, 695, 722, 769, 1467
BciVI GTATCC 1 cut(s) 1514
BclI TGATCA 1 cut(s) 1372
BcnI CCSGG 2 cut(s) 532, 654
BcoDI GTCTC 2 cut(s) 608, 758
BfaI CTAG 4 cut(s) 447, 950, 1770, 1780
BfuI GTATCC 1 cut(s) 1514
BisI GCNGC 2 cut(s) 137, 180
BlsI GCNGC 2 cut(s) 138, 181
Bme1390I CCNGG 9 cut(s) 393, 532, 540, 654, 664, 695, 722, 769, 1467
BmeT110I CYCGRG 2 cut(s) 429, 1424
BmgBI CACGTC 1 cut(s) 174
BmgT120I GGNCC 1 cut(s) 389
BmiI GGNNCC 2 cut(s) 1173, 1322
BmrFI CCNGG 9 cut(s) 393, 532, 540, 654, 664, 695, 722, 769, 1467
BmsI GCATC 6 cut(s) 81, 340, 603, 658, 895, 1462
BplI GAGNNNNNCTC 2 cut(s) 294, 326
Bpu10I CCTNAGC 1 cut(s) 158
BpuEI CTTGAG 3 cut(s) 296, 356, 1559
BpuMI CCSGG 2 cut(s) 532, 654
Bsa29I ATCGAT 1 cut(s) 1456
BsaBI GATNNNNATC 1 cut(s) 1170
BsaHI GRCGYC 1 cut(s) 658
BsaI GGTCTC 1 cut(s) 758
BsaJI CCNNGG 4 cut(s) 354, 391, 392, 662
BsaWI WCCGGW 3 cut(s) 48, 1258, 1589
BsaXI ACNNNNNCTCC 2 cut(s) 588, 618
Bsc4I CCNNNNNNNGG 5 cut(s) 55, 325, 393, 1658, 1661
Bse1I ACTGG 1 cut(s) 102
Bse3DI GCAATG 4 cut(s) 225, 921, 1397, 1611
Bse8I GATNNNNATC 1 cut(s) 1170
BseAI TCCGGA 2 cut(s) 48, 1258
BseBI CCWGG 7 cut(s) 393, 540, 664, 695, 722, 769, 1467
BseCI ATCGAT 1 cut(s) 1456
BseDI CCNNGG 4 cut(s) 354, 391, 392, 662
BseGI GGATG 4 cut(s) 649, 1420, 1621, 1728
BseJI GATNNNNATC 1 cut(s) 1170
BseLI CCNNNNNNNGG 5 cut(s) 55, 325, 393, 1658, 1661
BseMI GCAATG 4 cut(s) 225, 921, 1397, 1611
BseMII CTCAG 5 cut(s) 84, 172, 174, 1020, 1083
BseNI ACTGG 1 cut(s) 102
BseRI GAGGAG 1 cut(s) 299
BseXI GCAGC 1 cut(s) 191
BseYI CCCAGC 2 cut(s) 68, 1775
Bsh1236I CGCG 5 cut(s) 154, 510, 871, 972, 1304
BshFI GGCC 4 cut(s) 59, 306, 390, 558
BshVI ATCGAT 1 cut(s) 1456
BsiHKAI GWGCWC 4 cut(s) 39, 430, 1089, 1636
BsiHKCI CYCGRG 2 cut(s) 429, 1424
BsiSI CCGG 6 cut(s) 49, 387, 531, 653, 1259, 1590
BslFI GGGAC 2 cut(s) 645, 669
BslI CCNNNNNNNGG 5 cut(s) 55, 325, 393, 1658, 1661
BsmAI GTCTC 2 cut(s) 608, 758
BsmFI GGGAC 2 cut(s) 645, 669
BsnI GGCC 4 cut(s) 59, 306, 390, 558
Bso31I GGTCTC 1 cut(s) 758
BsoBI CYCGRG 2 cut(s) 429, 1424
Bsp1286I GDGCHC 4 cut(s) 39, 430, 1089, 1636
Bsp13I TCCGGA 2 cut(s) 48, 1258
BspACI CCGC 7 cut(s) 5, 40, 134, 137, 152, 510, 1684
BspANI GGCC 4 cut(s) 59, 306, 390, 558
BspCNI CTCAG 5 cut(s) 85, 171, 175, 1021, 1084
BspDI ATCGAT 1 cut(s) 1456
BspEI TCCGGA 2 cut(s) 48, 1258
BspFNI CGCG 5 cut(s) 154, 510, 871, 972, 1304
BspHI TCATGA 1 cut(s) 369
BspLI GGNNCC 2 cut(s) 1173, 1322
BspPI GGATC 9 cut(s) 123, 375, 484, 1166, 1179, 1272, 1315, 1328, 1764
BspQI GCTCTTC 2 cut(s) 27, 1380
BspTNI GGTCTC 1 cut(s) 758
BsrDI GCAATG 4 cut(s) 225, 921, 1397, 1611
BsrI ACTGG 1 cut(s) 102
BssECI CCNNGG 4 cut(s) 354, 391, 392, 662
BssNI GRCGYC 1 cut(s) 658
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 7 cut(s) 393, 540, 664, 695, 722, 769, 1467
Bst4CI ACNGT 4 cut(s) 622, 683, 993, 1289
Bst6I CTCTTC 3 cut(s) 27, 1274, 1380
BstACI GRCGYC 1 cut(s) 658
BstC8I GCNNGC 3 cut(s) 454, 1306, 1502
BstDEI CTNAG 6 cut(s) 93, 158, 183, 592, 1029, 1092
BstENI CCTNNNNNAGG 1 cut(s) 1656
BstF5I GGATG 4 cut(s) 649, 1420, 1621, 1728
BstFNI CGCG 5 cut(s) 154, 510, 871, 972, 1304
BstHHI GCGC 4 cut(s) 156, 510, 871, 921
BstMAI GTCTC 2 cut(s) 608, 758
BstMWI GCNNNNNNNGC 4 cut(s) 188, 564, 860, 1683
BstNI CCWGG 7 cut(s) 393, 540, 664, 695, 722, 769, 1467
BstNSI RCATGY 1 cut(s) 919
BstSCI CCNGG 9 cut(s) 391, 530, 538, 652, 662, 693, 720, 767, 1465
BstUI CGCG 5 cut(s) 154, 510, 871, 972, 1304
BstV1I GCAGC 1 cut(s) 191
BstX2I RGATCY 5 cut(s) 380, 489, 1171, 1264, 1320
BstYI RGATCY 5 cut(s) 380, 489, 1171, 1264, 1320
Bsu15I ATCGAT 1 cut(s) 1456
BsuI GTATCC 1 cut(s) 1514
BsuRI GGCC 4 cut(s) 59, 306, 390, 558
BsuTUI ATCGAT 1 cut(s) 1456
BtrI CACGTC 1 cut(s) 174
BtsCI GGATG 4 cut(s) 649, 1420, 1621, 1728
BtsIMutI CAGTG 4 cut(s) 1089, 1279, 1294, 1524
Cac8I GCNNGC 3 cut(s) 454, 1306, 1502
CaiI CAGNNNCTG 1 cut(s) 182
CciI TCATGA 1 cut(s) 369
CfoI GCGC 4 cut(s) 156, 510, 871, 921
Cfr13I GGNCC 1 cut(s) 389
ClaI ATCGAT 1 cut(s) 1456
CseI GACGC 3 cut(s) 704, 860, 892
Csp6I GTAC 2 cut(s) 25, 434
CviQI GTAC 2 cut(s) 25, 434
DdeI CTNAG 6 cut(s) 93, 158, 183, 592, 1029, 1092
DraI TTTAAA 1 cut(s) 687
EaeI YGGCCR 2 cut(s) 57, 556
Eam1104I CTCTTC 3 cut(s) 27, 1274, 1380
EarI CTCTTC 3 cut(s) 27, 1274, 1380
EciI GGCGGA 1 cut(s) 29
Ecl136II GAGCTC 4 cut(s) 37, 428, 1087, 1634
Eco130I CCWWGG 1 cut(s) 354
Eco147I AGGCCT 1 cut(s) 306
Eco24I GRGCYC 4 cut(s) 39, 430, 1089, 1636
Eco31I GGTCTC 1 cut(s) 758
Eco53kI GAGCTC 4 cut(s) 37, 428, 1087, 1634
Eco57I CTGAAG 4 cut(s) 660, 1255, 1316, 1749
Eco88I CYCGRG 2 cut(s) 429, 1424
EcoICRI GAGCTC 4 cut(s) 37, 428, 1087, 1634
EcoNI CCTNNNNNAGG 1 cut(s) 1656
EcoRI GAATTC 2 cut(s) 479, 1046
EcoRII CCWGG 7 cut(s) 391, 538, 662, 693, 720, 767, 1465
EcoT14I CCWWGG 1 cut(s) 354
EcoT38I GRGCYC 4 cut(s) 39, 430, 1089, 1636
ErhI CCWWGG 1 cut(s) 354
FaqI GGGAC 2 cut(s) 645, 669
FbaI TGATCA 1 cut(s) 1372
Fnu4HI GCNGC 2 cut(s) 137, 180
FokI GGATG 4 cut(s) 636, 1407, 1628, 1715
FriOI GRGCYC 4 cut(s) 39, 430, 1089, 1636
Fsp4HI GCNGC 2 cut(s) 137, 180
FspAI RTGCGCAY 1 cut(s) 920
FspBI CTAG 4 cut(s) 447, 950, 1770, 1780
FspI TGCGCA 1 cut(s) 920
GlaI GCGC 4 cut(s) 155, 509, 870, 920
GluI GCNGC 2 cut(s) 137, 180
GsaI CCCAGC 2 cut(s) 72, 1779
HaeIII GGCC 4 cut(s) 59, 306, 390, 558
HapII CCGG 6 cut(s) 49, 387, 531, 653, 1259, 1590
HgaI GACGC 3 cut(s) 704, 860, 892
HhaI GCGC 4 cut(s) 156, 510, 871, 921
Hin1I GRCGYC 1 cut(s) 658
Hin6I GCGC 4 cut(s) 154, 508, 869, 919
HinP1I GCGC 4 cut(s) 154, 508, 869, 919
HindIII AAGCTT 1 cut(s) 1364
HinfI GANTC 8 cut(s) 14, 257, 350, 667, 1399, 1435, 1560, 1706
HpaII CCGG 6 cut(s) 49, 387, 531, 653, 1259, 1590
HphI GGTGA 5 cut(s) 138, 431, 524, 710, 1604
Hpy166II GTNNAC 2 cut(s) 1331, 1714
Hpy188I TCNGA 6 cut(s) 94, 463, 478, 1017, 1205, 1296
Hpy8I GTNNAC 2 cut(s) 1331, 1714
HpyAV CCTTC 6 cut(s) 181, 286, 1291, 1613, 1744, 1773
HpyCH4III ACNGT 4 cut(s) 622, 683, 993, 1289
HpyCH4IV ACGT 4 cut(s) 173, 342, 658, 751
HpyCH4V TGCA 5 cut(s) 331, 575, 926, 1064, 1241
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 564, 860, 1683
HpyF3I CTNAG 6 cut(s) 93, 158, 183, 592, 1029, 1092
HpySE526I ACGT 4 cut(s) 173, 342, 658, 751
Hsp92I GRCGYC 1 cut(s) 658
HspAI GCGC 4 cut(s) 154, 508, 869, 919
Kpn2I TCCGGA 2 cut(s) 48, 1258
Ksp22I TGATCA 1 cut(s) 1372
LguI GCTCTTC 2 cut(s) 27, 1380
LmnI GCTCC 4 cut(s) 42, 51, 860, 1084
Lsp1109I GCAGC 1 cut(s) 191
LweI GCATC 6 cut(s) 81, 340, 603, 658, 895, 1462
MaeI CTAG 4 cut(s) 447, 950, 1770, 1780
MaeII ACGT 4 cut(s) 173, 342, 658, 751
MaeIII GTNAC 5 cut(s) 169, 343, 512, 526, 716
MboII GAAGA 6 cut(s) 44, 135, 653, 794, 1261, 1397
MfeI CAATTG 1 cut(s) 1176
MflI RGATCY 5 cut(s) 380, 489, 1171, 1264, 1320
MhlI GDGCHC 4 cut(s) 39, 430, 1089, 1636
MlsI TGGCCA 2 cut(s) 59, 558
MluCI AATT 9 cut(s) 220, 438, 457, 479, 963, 1046, 1176, 1226, 1487
MluI ACGCGT 1 cut(s) 970
MluNI TGGCCA 2 cut(s) 59, 558
MlyI GAGTC 1 cut(s) 1444
MmeI TCCRAC 3 cut(s) 1427, 1573, 1679
Mox20I TGGCCA 2 cut(s) 59, 558
MroI TCCGGA 2 cut(s) 48, 1258
MscI TGGCCA 2 cut(s) 59, 558
MseI TTAA 8 cut(s) 486, 522, 686, 728, 942, 1229, 1700, 1719
MslI CAYNNNNRTG 3 cut(s) 498, 891, 1155
Msp20I TGGCCA 2 cut(s) 59, 558
MspA1I CMGCKG 2 cut(s) 182, 854
MspI CCGG 6 cut(s) 49, 387, 531, 653, 1259, 1590
MspR9I CCNGG 9 cut(s) 393, 532, 540, 654, 664, 695, 722, 769, 1467
MunI CAATTG 1 cut(s) 1176
MvaI CCWGG 7 cut(s) 393, 540, 664, 695, 722, 769, 1467
MvnI CGCG 5 cut(s) 154, 510, 871, 972, 1304
MwoI GCNNNNNNNGC 4 cut(s) 188, 564, 860, 1683
NciI CCSGG 2 cut(s) 532, 654
NlaIV GGNNCC 2 cut(s) 1173, 1322
NmuCI GTSAC 5 cut(s) 169, 343, 512, 526, 716
NsbI TGCGCA 1 cut(s) 920
NspI RCATGY 1 cut(s) 919
PagI TCATGA 1 cut(s) 369
PasI CCCWGGG 1 cut(s) 392
PceI AGGCCT 1 cut(s) 306
PciSI GCTCTTC 2 cut(s) 27, 1380
PcsI WCGNNNNNNNCGW 1 cut(s) 817
PfeI GAWTC 7 cut(s) 14, 257, 350, 667, 1399, 1560, 1706
PflMI CCANNNNNTGG 1 cut(s) 1661
PfoI TCCNGGA 1 cut(s) 652
PkrI GCNGC 2 cut(s) 138, 181
PleI GAGTC 1 cut(s) 1443
PpsI GAGTC 1 cut(s) 1443
PsiI TTATAA 1 cut(s) 1412
Psp124BI GAGCTC 4 cut(s) 39, 430, 1089, 1636
Psp6I CCWGG 7 cut(s) 391, 538, 662, 693, 720, 767, 1465
PspFI CCCAGC 2 cut(s) 68, 1775
PspGI CCWGG 7 cut(s) 391, 538, 662, 693, 720, 767, 1465
PspN4I GGNNCC 2 cut(s) 1173, 1322
PspPI GGNCC 1 cut(s) 389
PstNI CAGNNNCTG 1 cut(s) 182
PsuI RGATCY 5 cut(s) 380, 489, 1171, 1264, 1320
PvuII CAGCTG 2 cut(s) 182, 854
RsaI GTAC 2 cut(s) 26, 435
RsaNI GTAC 2 cut(s) 25, 434
RseI CAYNNNNRTG 3 cut(s) 498, 891, 1155
SacI GAGCTC 4 cut(s) 39, 430, 1089, 1636
SapI GCTCTTC 2 cut(s) 27, 1380
SaqAI TTAA 8 cut(s) 486, 522, 686, 728, 942, 1229, 1700, 1719
SatI GCNGC 2 cut(s) 137, 180
Sau96I GGNCC 1 cut(s) 389
SchI GAGTC 1 cut(s) 1444
ScrFI CCNGG 9 cut(s) 393, 532, 540, 654, 664, 695, 722, 769, 1467
SduI GDGCHC 4 cut(s) 39, 430, 1089, 1636
SfaNI GCATC 6 cut(s) 81, 340, 603, 658, 895, 1462
SmiMI CAYNNNNRTG 3 cut(s) 498, 891, 1155
SmlI CTYRAG 3 cut(s) 311, 335, 1574
SmoI CTYRAG 3 cut(s) 311, 335, 1574
Sse9I AATT 9 cut(s) 220, 438, 457, 479, 963, 1046, 1176, 1226, 1487
SseBI AGGCCT 1 cut(s) 306
SsiI CCGC 7 cut(s) 5, 40, 134, 137, 152, 510, 1684
SspI AATATT 1 cut(s) 840
SspMI CTAG 4 cut(s) 447, 950, 1770, 1780
SstI GAGCTC 4 cut(s) 39, 430, 1089, 1636
StuI AGGCCT 1 cut(s) 306
StyD4I CCNGG 9 cut(s) 391, 530, 538, 652, 662, 693, 720, 767, 1465
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 4 cut(s) 622, 683, 993, 1289
TaiI ACGT 4 cut(s) 176, 345, 661, 754
TaqI TCGA 2 cut(s) 424, 1456
TasI AATT 9 cut(s) 220, 438, 457, 479, 963, 1046, 1176, 1226, 1487
TauI GCSGC 1 cut(s) 139
TfiI GAWTC 7 cut(s) 14, 257, 350, 667, 1399, 1560, 1706
Tru1I TTAA 8 cut(s) 486, 522, 686, 728, 942, 1229, 1700, 1719
Tru9I TTAA 8 cut(s) 486, 522, 686, 728, 942, 1229, 1700, 1719
TscAI CASTG 4 cut(s) 1096, 1279, 1294, 1531
TseFI GTSAC 5 cut(s) 169, 343, 512, 526, 716
TseI GCWGC 1 cut(s) 179
Tsp45I GTSAC 5 cut(s) 169, 343, 512, 526, 716
TspDTI ATGAA 8 cut(s) 222, 270, 386, 794, 909, 1614, 1632, 1760
TspGWI ACGGA 2 cut(s) 809, 1222
TspRI CASTG 4 cut(s) 1096, 1279, 1294, 1531
Van91I CCANNNNNTGG 1 cut(s) 1661
XagI CCTNNNNNAGG 1 cut(s) 1656
XapI RAATTY 3 cut(s) 438, 479, 1046
XceI RCATGY 1 cut(s) 919
XcmI CCANNNNNNNNNTGG 2 cut(s) 25, 1446
XspI CTAG 4 cut(s) 447, 950, 1770, 1780
ZraI GACGTC 1 cut(s) 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.