Rh5DG472500

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0023115
Physical Location & Seq
Forward (+)
74703839 .. 74726959
23121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG472500.1

Sequence Viewer

Length: 1023 bp
ATGAAGGTTCTGAATCTGGAAATGGGTTTTGTTGAGTTCATGGCACTTGAACAAGGAGTTGGATCCATTTGTCTGTTCCCATCGACAAACCCTTCCACTCTTTTCACTCCGCTCTCATTCCCTCCCCCCAAACTCAGTGCTCCTCGTTATCGGATGCTTCCCACTGAGACTGACCTCAACAGCCCTCCGCTCCTTTCCAGTTTTCCCGATAAGATTCTTCCCGTCCATTCCGGAGATAAAGCCTGGGATGGCGGTGCTGTCACATCAAATCTTGCTAGGAAATGTGAGGCCCTGGCTGTTTCTGGTTTAGTTGAGTATGGAGATGAGATTGATGTAATTGCTCCCTCTGACATTCTCAAGCAGATATTCAAAATGCCATATTCAAAGGCTCGGCTTTCCATTGCAGTTCAGCGGATTGGGCACACACTTATTCTTAATACCGGGCCTGATGCTGAAGAGGGAGAAAAGTTGATCAGGAGACGTCAAAATCAGTCAAAATGTCTTGATCAATCTTTGTTTTTGAATTTCGCCATGCATTCTGTTCGAATGGAAGCTTGTGATTGCCCACCAACTCATCATGTCGCCTCAAATGAGCAGTCAAATGCTTCTGTTCTCCCTGGAGTGAATACTGGTTTTGTGGGACAGCATCATCATGTAGCTCGGGGTGAAGGGACAAAGCATACTTCAGAGTATACACAAGTTAAACAAGATGAATTCGTTTGGGCAAGTAAGAAAAATAAAAACAAATCGGGACATAATGCTGTGAAAAAAGCTTCGCAAGTTGGTGACAAGCCCGTGTCTTCGATGCAAGAGTCTGAAAAACATAAACGAGGTGATAACGATGAGTTTTTACGGAGCAACTCAAAGCATGCATCTCTCACTGCAACTAAGGAGGAAGATGATTCGCATGTATTGGGATTTGGGGCATGGGGTGGAATTGGAAAAGCAGCGATTGCATATATATCCCTGTGGGTTCTTGTAATTGAACATGATCTGCCAGCTTCTATTCTTAGTAGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

340

Amino Acids

37.18

Weight (kDa)

6.92

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDRF1_N PF23788 106 - 146 1e-07 EDRF1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023115)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0005786.1_g000001 Rmu_sc0014039.1_g000008
rosa_samantha Rh5DG472500
rosa_wichuraiana Rw0G011040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 484
AccBSI CCGCTC 2 cut(s) 112, 190
AccI GTMKAC 1 cut(s) 692
AccIII TCCGGA 1 cut(s) 230
AciI CCGC 4 cut(s) 110, 188, 252, 412
AclWI GGATC 2 cut(s) 57, 70
AcsI RAATTY 2 cut(s) 523, 713
AcuI CTGAAG 2 cut(s) 474, 669
AcyI GRCGYC 1 cut(s) 481
AgsI TTSAA 5 cut(s) 50, 370, 384, 523, 986
AjnI CCWGG 3 cut(s) 242, 291, 616
AjuI GAANNNNNNNTTGG 2 cut(s) 42, 74
AluBI AGCT 4 cut(s) 554, 659, 773, 1001
AluI AGCT 4 cut(s) 554, 659, 773, 1001
Alw21I GWGCWC 1 cut(s) 142
Alw26I GTCTC 2 cut(s) 161, 472
AlwI GGATC 2 cut(s) 57, 70
Ama87I CYCGRG 1 cut(s) 660
Aor13HI TCCGGA 1 cut(s) 230
AoxI GGCC 2 cut(s) 288, 443
ApeKI GCWGC 1 cut(s) 947
ApoI RAATTY 2 cut(s) 523, 713
ArsI GACNNNNNNTTYG 2 cut(s) 581, 613
AspS9I GGNCC 2 cut(s) 289, 443
AsuC2I CCSGG 1 cut(s) 442
AsuHPI GGTGA 3 cut(s) 677, 797, 845
AsuII TTCGAA 1 cut(s) 544
AvaI CYCGRG 1 cut(s) 660
BaeGI GKGCMC 1 cut(s) 423
BamHI GGATCC 1 cut(s) 62
BbsI GAAGAC 1 cut(s) 792
Bbv12I GWGCWC 1 cut(s) 142
BbvI GCAGC 1 cut(s) 959
BccI CCATC 2 cut(s) 88, 242
BcgI CGANNNNNNTGC 2 cut(s) 524, 558
BciT130I CCWGG 3 cut(s) 244, 293, 618
BclI TGATCA 2 cut(s) 471, 505
BcnI CCSGG 1 cut(s) 442
BcoDI GTCTC 2 cut(s) 161, 472
BfaI CTAG 1 cut(s) 276
BisI GCNGC 1 cut(s) 948
BlsI GCNGC 1 cut(s) 949
Bme1390I CCNGG 4 cut(s) 244, 293, 442, 618
BmeT110I CYCGRG 1 cut(s) 660
BmgT120I GGNCC 2 cut(s) 289, 443
BmiI GGNNCC 1 cut(s) 64
BmrFI CCNGG 4 cut(s) 244, 293, 442, 618
BmsI GCATC 5 cut(s) 144, 439, 655, 795, 881
BpiI GAAGAC 1 cut(s) 792
BpmI CTGGAG 1 cut(s) 639
Bpu14I TTCGAA 1 cut(s) 544
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 442
BsaHI GRCGYC 1 cut(s) 481
BsaJI CCNNGG 3 cut(s) 243, 291, 616
BsaWI WCCGGW 1 cut(s) 230
Bse1I ACTGG 2 cut(s) 198, 634
Bse3DI GCAATG 1 cut(s) 399
BseAI TCCGGA 1 cut(s) 230
BseBI CCWGG 3 cut(s) 244, 293, 618
BseDI CCNNGG 3 cut(s) 243, 291, 616
BseGI GGATG 2 cut(s) 159, 253
BseMI GCAATG 1 cut(s) 399
BseMII CTCAG 2 cut(s) 148, 156
BseNI ACTGG 2 cut(s) 198, 634
BseRI GAGGAG 1 cut(s) 132
BseSI GKGCMC 1 cut(s) 423
BseXI GCAGC 1 cut(s) 959
BshFI GGCC 2 cut(s) 290, 445
BsiHKAI GWGCWC 1 cut(s) 142
BsiHKCI CYCGRG 1 cut(s) 660
BsiSI CCGG 2 cut(s) 231, 441
BslFI GGGAC 3 cut(s) 654, 685, 765
BsmAI GTCTC 2 cut(s) 161, 472
BsmBI CGTCTC 1 cut(s) 472
BsmFI GGGAC 3 cut(s) 654, 685, 765
BsmI GAATGC 1 cut(s) 535
BsnI GGCC 2 cut(s) 290, 445
BsoBI CYCGRG 1 cut(s) 660
Bsp119I TTCGAA 1 cut(s) 544
Bsp1286I GDGCHC 2 cut(s) 142, 423
Bsp13I TCCGGA 1 cut(s) 230
Bsp143I GATC 4 cut(s) 62, 471, 505, 991
BspACI CCGC 4 cut(s) 110, 188, 252, 412
BspANI GGCC 2 cut(s) 290, 445
BspCNI CTCAG 2 cut(s) 147, 157
BspEI TCCGGA 1 cut(s) 230
BspLI GGNNCC 1 cut(s) 64
BspPI GGATC 2 cut(s) 57, 70
BspT104I TTCGAA 1 cut(s) 544
BsrBI CCGCTC 2 cut(s) 112, 190
BsrDI GCAATG 1 cut(s) 399
BsrI ACTGG 2 cut(s) 198, 634
BssECI CCNNGG 3 cut(s) 243, 291, 616
BssMI GATC 4 cut(s) 62, 471, 505, 991
BssNAI GTATAC 1 cut(s) 693
BssNI GRCGYC 1 cut(s) 481
Bst1107I GTATAC 1 cut(s) 693
Bst2UI CCWGG 3 cut(s) 244, 293, 618
Bst6I CTCTTC 1 cut(s) 450
BstACI GRCGYC 1 cut(s) 481
BstAPI GCANNNNNTGC 1 cut(s) 953
BstBI TTCGAA 1 cut(s) 544
BstC8I GCNNGC 2 cut(s) 870, 999
BstDEI CTNAG 4 cut(s) 134, 165, 888, 1010
BstF5I GGATG 2 cut(s) 159, 253
BstKTI GATC 4 cut(s) 65, 474, 508, 994
BstMAI GTCTC 2 cut(s) 161, 472
BstMBI GATC 4 cut(s) 62, 471, 505, 991
BstMWI GCNNNNNNNGC 2 cut(s) 418, 953
BstNI CCWGG 3 cut(s) 244, 293, 618
BstNSI RCATGY 2 cut(s) 872, 911
BstSCI CCNGG 4 cut(s) 242, 291, 440, 616
BstSLI GKGCMC 1 cut(s) 423
BstV1I GCAGC 1 cut(s) 959
BstV2I GAAGAC 1 cut(s) 792
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
BstZ17I GTATAC 1 cut(s) 693
BsuRI GGCC 2 cut(s) 290, 445
BtsCI GGATG 2 cut(s) 159, 253
BtsI GCAGTG 1 cut(s) 879
BtsIMutI CAGTG 3 cut(s) 142, 162, 879
Cac8I GCNNGC 2 cut(s) 870, 999
Cfr13I GGNCC 2 cut(s) 289, 443
CviAII CATG 8 cut(s) 40, 532, 578, 653, 869, 908, 927, 989
DdeI CTNAG 4 cut(s) 134, 165, 888, 1010
DpnI GATC 4 cut(s) 64, 473, 507, 993
DpnII GATC 4 cut(s) 62, 471, 505, 991
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
Eco57I CTGAAG 2 cut(s) 474, 669
Eco88I CYCGRG 1 cut(s) 660
EcoO109I RGGNCCY 1 cut(s) 289
EcoRI GAATTC 1 cut(s) 713
EcoRII CCWGG 3 cut(s) 242, 291, 616
EcoT22I ATGCAT 2 cut(s) 537, 874
Esp3I CGTCTC 1 cut(s) 472
FaeI CATG 8 cut(s) 43, 535, 581, 656, 872, 911, 930, 992
FaqI GGGAC 3 cut(s) 654, 685, 765
FatI CATG 8 cut(s) 39, 531, 577, 652, 868, 907, 926, 988
FbaI TGATCA 2 cut(s) 471, 505
FblI GTMKAC 1 cut(s) 692
Fnu4HI GCNGC 1 cut(s) 948
FokI GGATG 2 cut(s) 166, 260
Fsp4HI GCNGC 1 cut(s) 948
FspBI CTAG 1 cut(s) 276
GluI GCNGC 1 cut(s) 948
GsuI CTGGAG 1 cut(s) 639
HaeIII GGCC 2 cut(s) 290, 445
HapII CCGG 2 cut(s) 231, 441
Hin1I GRCGYC 1 cut(s) 481
Hin1II CATG 8 cut(s) 43, 535, 581, 656, 872, 911, 930, 992
HindIII AAGCTT 2 cut(s) 552, 771
HinfI GANTC 4 cut(s) 13, 214, 812, 902
HpaII CCGG 2 cut(s) 231, 441
HphI GGTGA 3 cut(s) 677, 797, 845
Hpy166II GTNNAC 1 cut(s) 693
Hpy188I TCNGA 5 cut(s) 12, 153, 349, 688, 817
Hpy188III TCNNGA 6 cut(s) 17, 206, 231, 475, 503, 750
Hpy8I GTNNAC 1 cut(s) 693
HpyAV CCTTC 2 cut(s) 102, 662
HpyCH4IV ACGT 1 cut(s) 481
HpyCH4V TGCA 6 cut(s) 404, 535, 808, 872, 884, 956
HpyF10VI GCNNNNNNNGC 2 cut(s) 418, 953
HpyF3I CTNAG 4 cut(s) 134, 165, 888, 1010
HpySE526I ACGT 1 cut(s) 481
Hsp92I GRCGYC 1 cut(s) 481
Hsp92II CATG 8 cut(s) 43, 535, 581, 656, 872, 911, 930, 992
Kpn2I TCCGGA 1 cut(s) 230
Ksp22I TGATCA 2 cut(s) 471, 505
Kzo9I GATC 4 cut(s) 62, 471, 505, 991
LmnI GCTCC 4 cut(s) 145, 195, 346, 855
Lsp1109I GCAGC 1 cut(s) 959
LweI GCATC 5 cut(s) 144, 439, 655, 795, 881
MaeI CTAG 1 cut(s) 276
MaeII ACGT 1 cut(s) 481
MaeIII GTNAC 2 cut(s) 259, 785
MalI GATC 4 cut(s) 64, 473, 507, 993
MbiI CCGCTC 2 cut(s) 112, 190
MboI GATC 4 cut(s) 62, 471, 505, 991
MboII GAAGA 4 cut(s) 209, 467, 792, 908
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 2 cut(s) 142, 423
MluCI AATT 5 cut(s) 336, 523, 713, 936, 981
MlyI GAGTC 1 cut(s) 821
MmeI TCCRAC 1 cut(s) 40
Mph1103I ATGCAT 2 cut(s) 537, 874
MroI TCCGGA 1 cut(s) 230
MseI TTAA 2 cut(s) 435, 702
MslI CAYNNNNRTG 1 cut(s) 651
MspA1I CMGCKG 1 cut(s) 412
MspI CCGG 2 cut(s) 231, 441
MspR9I CCNGG 4 cut(s) 244, 293, 442, 618
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 3 cut(s) 244, 293, 618
MwoI GCNNNNNNNGC 2 cut(s) 418, 953
NciI CCSGG 1 cut(s) 442
NdeII GATC 4 cut(s) 62, 471, 505, 991
NlaIII CATG 8 cut(s) 43, 535, 581, 656, 872, 911, 930, 992
NlaIV GGNNCC 1 cut(s) 64
NmeAIII GCCGAG 1 cut(s) 370
NmuCI GTSAC 2 cut(s) 259, 785
NsiI ATGCAT 2 cut(s) 537, 874
NspI RCATGY 2 cut(s) 872, 911
NspV TTCGAA 1 cut(s) 544
PaeI GCATGC 1 cut(s) 872
PctI GAATGC 1 cut(s) 535
PfeI GAWTC 3 cut(s) 13, 214, 902
PkrI GCNGC 1 cut(s) 949
PleI GAGTC 1 cut(s) 820
PpsI GAGTC 1 cut(s) 820
Psp6I CCWGG 3 cut(s) 242, 291, 616
PspGI CCWGG 3 cut(s) 242, 291, 616
PspN4I GGNNCC 1 cut(s) 64
PspPI GGNCC 2 cut(s) 289, 443
PsuI RGATCY 1 cut(s) 62
RseI CAYNNNNRTG 1 cut(s) 651
SaqAI TTAA 2 cut(s) 435, 702
SatI GCNGC 1 cut(s) 948
Sau3AI GATC 4 cut(s) 62, 471, 505, 991
Sau96I GGNCC 2 cut(s) 289, 443
SchI GAGTC 1 cut(s) 821
ScrFI CCNGG 4 cut(s) 244, 293, 442, 618
SduI GDGCHC 2 cut(s) 142, 423
SetI ASST 8 cut(s) 9, 177, 484, 556, 661, 775, 835, 1003
SfaNI GCATC 5 cut(s) 144, 439, 655, 795, 881
SfuI TTCGAA 1 cut(s) 544
SmiMI CAYNNNNRTG 1 cut(s) 651
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
SphI GCATGC 1 cut(s) 872
Sse9I AATT 5 cut(s) 336, 523, 713, 936, 981
SsiI CCGC 4 cut(s) 110, 188, 252, 412
SspMI CTAG 1 cut(s) 276
StyD4I CCNGG 4 cut(s) 242, 291, 440, 616
TaiI ACGT 1 cut(s) 484
TaqI TCGA 3 cut(s) 83, 544, 803
TasI AATT 5 cut(s) 336, 523, 713, 936, 981
TfiI GAWTC 3 cut(s) 13, 214, 902
Tru1I TTAA 2 cut(s) 435, 702
Tru9I TTAA 2 cut(s) 435, 702
TscAI CASTG 3 cut(s) 142, 169, 886
TseFI GTSAC 2 cut(s) 259, 785
TseI GCWGC 1 cut(s) 947
Tsp45I GTSAC 2 cut(s) 259, 785
TspDTI ATGAA 3 cut(s) 17, 28, 726
TspGWI ACGGA 1 cut(s) 868
TspRI CASTG 3 cut(s) 142, 169, 886
XapI RAATTY 2 cut(s) 523, 713
XceI RCATGY 2 cut(s) 872, 911
XmiI GTMKAC 1 cut(s) 692
XspI CTAG 1 cut(s) 276
ZraI GACGTC 1 cut(s) 482
Zsp2I ATGCAT 2 cut(s) 537, 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.