Rh6BG327700

Protein ALTERED XYLOGLUCAN

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0024609
Physical Location & Seq
Forward (+)
56058732 .. 56060258
1527 bp
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UTR
Exon/CDS
Intron
Rh6BG327700.1

Sequence Viewer

Length: 789 bp
ATGGATTTGTTAAGCACAATGCTGAAGGACTTGAATATAATATGGTCCTATTGGTGGCAGGTACTGCATTTCTCTAATAAAACTACTTTCTTCCCCCCAAAAAAAATTATACTACATGAATACTCTCTCCTTACCACTCAACATGAGACAGAAATGCAATCTGCCACCATAGCTTATAAGCATGCCAAACAAATCAGAAAGAAAAATAATACAATGCCTTATCCGAATCAGGTAAAGCATGTATCCAGCCAATCATTCAAGTTTGGTTCAGCTCTTGGGTGTCATTTCTGTCCTGGTCTAAATCACACTGAGATTGGCTTCTATGATGTTCTGAGAAAGGCCGTAAGGACTACCCTAAAGACCATAACTGAAAGGAGAGGGACAAGTGAGAATGGGATCAATGTAATTGTGACTACATTTTCACCAGCTCATTTTGAAGGTGAGTGGGACAAGGCTGGGGCTTGTCCCAAGACCAAGCCTTACAACAAGGGAGAGAAAAATCTTGAAGGAATGGATGCTGAGATGAGGCAAGTTGAGGTGGAAGAAGTGGAAGCTGCTAAGGTGAATGGTAAAGGGTTTTCAGGGTTTAAGGTTGAAGAATTGGATGTGAGCTATTTGTCATTGATGAGGCCAAATGGGCACCCTGGGCCTTATATGAACCCATTTCTATTTTTTAATGGGGTGAAAGAGAGGGTGCAAAATGATTGTGTACATTGGTGTTTGCCTGGGGCTATTGACACATGGAATGAGATATTGCTAGAGATCATGAAGAAATGGAATCGGGGTTGA

Protein Analysis

262

Amino Acids

30.08

Weight (kDa)

8.68

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 97 - 254 4.4e-40 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024609)

Species Orthologous Gene IDs
pyrus_communis pycom01g10620 pycom13g07570
rosa_samantha Rh6BG327700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
Acc36I ACCTGC 1 cut(s) 49
AccB1I GGYRCC 1 cut(s) 639
AclWI GGATC 1 cut(s) 404
AcuI CTGAAG 1 cut(s) 44
AfaI GTAC 2 cut(s) 63, 711
AfiI CCNNNNNNNGG 1 cut(s) 54
AgsI TTSAA 5 cut(s) 34, 259, 437, 506, 596
AjnI CCWGG 3 cut(s) 292, 643, 724
AluBI AGCT 5 cut(s) 173, 272, 428, 554, 612
AluI AGCT 5 cut(s) 173, 272, 428, 554, 612
Alw26I GTCTC 1 cut(s) 140
AlwI GGATC 1 cut(s) 404
AlwNI CAGNNNCTG 1 cut(s) 64
AoxI GGCC 3 cut(s) 339, 629, 647
ApeKI GCWGC 1 cut(s) 554
AspS9I GGNCC 2 cut(s) 45, 647
AsuHPI GGTGA 4 cut(s) 414, 452, 574, 694
AvaII GGWCC 1 cut(s) 45
BaeGI GKGCMC 1 cut(s) 642
BanI GGYRCC 1 cut(s) 639
BbvI GCAGC 1 cut(s) 541
BceAI ACGGC 1 cut(s) 326
BciT130I CCWGG 3 cut(s) 294, 645, 726
BciVI GTATCC 1 cut(s) 253
BcoDI GTCTC 1 cut(s) 140
BfaI CTAG 1 cut(s) 758
BfuAI ACCTGC 1 cut(s) 49
BfuI GTATCC 1 cut(s) 253
BglI GCCNNNNNGGC 1 cut(s) 637
BisI GCNGC 1 cut(s) 555
BlsI GCNGC 1 cut(s) 556
Bme1390I CCNGG 3 cut(s) 294, 645, 726
Bme18I GGWCC 1 cut(s) 45
BmgT120I GGNCC 2 cut(s) 45, 647
BmiI GGNNCC 1 cut(s) 641
BmrFI CCNGG 3 cut(s) 294, 645, 726
BmsI GCATC 1 cut(s) 505
Bpu10I CCTNAGC 1 cut(s) 558
BsaJI CCNNGG 3 cut(s) 643, 644, 725
Bsc4I CCNNNNNNNGG 1 cut(s) 54
BseBI CCWGG 3 cut(s) 294, 645, 726
BseDI CCNNGG 3 cut(s) 643, 644, 725
BseGI GGATG 2 cut(s) 520, 610
BseLI CCNNNNNNNGG 1 cut(s) 54
BseMII CTCAG 3 cut(s) 300, 323, 510
BseSI GKGCMC 1 cut(s) 642
BseXI GCAGC 1 cut(s) 541
BseYI CCCAGC 1 cut(s) 455
BshFI GGCC 3 cut(s) 341, 631, 649
BshNI GGYRCC 1 cut(s) 639
BslFI GGGAC 3 cut(s) 394, 450, 461
BslI CCNNNNNNNGG 1 cut(s) 54
BsmAI GTCTC 1 cut(s) 140
BsmFI GGGAC 3 cut(s) 394, 450, 461
BsnI GGCC 3 cut(s) 341, 631, 649
Bsp1286I GDGCHC 1 cut(s) 642
Bsp1407I TGTACA 1 cut(s) 709
Bsp143I GATC 2 cut(s) 396, 762
BspANI GGCC 3 cut(s) 341, 631, 649
BspCNI CTCAG 3 cut(s) 301, 324, 511
BspHI TCATGA 1 cut(s) 765
BspLI GGNNCC 1 cut(s) 641
BspMI ACCTGC 1 cut(s) 49
BspPI GGATC 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 639
BsrGI TGTACA 1 cut(s) 709
BssECI CCNNGG 3 cut(s) 643, 644, 725
BssMI GATC 2 cut(s) 396, 762
Bst2UI CCWGG 3 cut(s) 294, 645, 726
BstAPI GCANNNNNTGC 1 cut(s) 64
BstAUI TGTACA 1 cut(s) 709
BstC8I GCNNGC 1 cut(s) 183
BstDEI CTNAG 4 cut(s) 309, 332, 519, 558
BstF5I GGATG 2 cut(s) 520, 610
BstKTI GATC 2 cut(s) 399, 765
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 2 cut(s) 396, 762
BstMWI GCNNNNNNNGC 4 cut(s) 64, 170, 637, 646
BstNI CCWGG 3 cut(s) 294, 645, 726
BstNSI RCATGY 2 cut(s) 185, 242
BstSCI CCNGG 3 cut(s) 292, 643, 724
BstSLI GKGCMC 1 cut(s) 642
BstV1I GCAGC 1 cut(s) 541
BsuI GTATCC 1 cut(s) 253
BsuRI GGCC 3 cut(s) 341, 631, 649
BtsCI GGATG 2 cut(s) 520, 610
BtsIMutI CAGTG 1 cut(s) 306
BveI ACCTGC 1 cut(s) 49
Cac8I GCNNGC 1 cut(s) 183
CaiI CAGNNNCTG 1 cut(s) 64
CciI TCATGA 1 cut(s) 765
Cfr13I GGNCC 2 cut(s) 45, 647
Csp6I GTAC 2 cut(s) 62, 710
CviAII CATG 6 cut(s) 116, 143, 182, 239, 741, 766
CviQI GTAC 2 cut(s) 62, 710
DdeI CTNAG 4 cut(s) 309, 332, 519, 558
DpnI GATC 2 cut(s) 398, 764
DpnII GATC 2 cut(s) 396, 762
Eco47I GGWCC 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 44
EcoRII CCWGG 3 cut(s) 292, 643, 724
FaeI CATG 6 cut(s) 119, 146, 185, 242, 744, 769
FaqI GGGAC 3 cut(s) 394, 450, 461
FatI CATG 6 cut(s) 115, 142, 181, 238, 740, 765
Fnu4HI GCNGC 1 cut(s) 555
FokI GGATG 2 cut(s) 527, 617
Fsp4HI GCNGC 1 cut(s) 555
FspBI CTAG 1 cut(s) 758
GluI GCNGC 1 cut(s) 555
GsaI CCCAGC 1 cut(s) 459
HaeIII GGCC 3 cut(s) 341, 631, 649
Hin1II CATG 6 cut(s) 119, 146, 185, 242, 744, 769
HinfI GANTC 2 cut(s) 226, 778
HphI GGTGA 4 cut(s) 414, 452, 574, 694
Hpy166II GTNNAC 1 cut(s) 710
Hpy188I TCNGA 3 cut(s) 197, 225, 333
Hpy188III TCNNGA 2 cut(s) 503, 766
Hpy8I GTNNAC 1 cut(s) 710
HpyAV CCTTC 3 cut(s) 19, 431, 500
HpyCH4V TGCA 3 cut(s) 67, 157, 697
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 170, 637, 646
HpyF3I CTNAG 4 cut(s) 309, 332, 519, 558
Hsp92II CATG 6 cut(s) 119, 146, 185, 242, 744, 769
Kzo9I GATC 2 cut(s) 396, 762
Lsp1109I GCAGC 1 cut(s) 541
LweI GCATC 1 cut(s) 505
MaeI CTAG 1 cut(s) 758
MaeIII GTNAC 1 cut(s) 409
MalI GATC 2 cut(s) 398, 764
MboI GATC 2 cut(s) 396, 762
MboII GAAGA 4 cut(s) 82, 554, 608, 781
MhlI GDGCHC 1 cut(s) 642
MluCI AATT 3 cut(s) 105, 405, 599
MnlI CCTC 5 cut(s) 371, 519, 529, 621, 684
MseI TTAA 3 cut(s) 11, 588, 675
MspR9I CCNGG 3 cut(s) 294, 645, 726
MvaI CCWGG 3 cut(s) 294, 645, 726
MwoI GCNNNNNNNGC 4 cut(s) 64, 170, 637, 646
NdeII GATC 2 cut(s) 396, 762
NlaIII CATG 6 cut(s) 119, 146, 185, 242, 744, 769
NlaIV GGNNCC 1 cut(s) 641
NmuCI GTSAC 1 cut(s) 409
NspI RCATGY 2 cut(s) 185, 242
PaeI GCATGC 1 cut(s) 185
PagI TCATGA 1 cut(s) 765
PasI CCCWGGG 1 cut(s) 644
PfeI GAWTC 2 cut(s) 226, 778
PkrI GCNGC 1 cut(s) 556
PsiI TTATAA 1 cut(s) 177
Psp6I CCWGG 3 cut(s) 292, 643, 724
PspFI CCCAGC 1 cut(s) 455
PspGI CCWGG 3 cut(s) 292, 643, 724
PspN4I GGNNCC 1 cut(s) 641
PspPI GGNCC 2 cut(s) 45, 647
PstNI CAGNNNCTG 1 cut(s) 64
RsaI GTAC 2 cut(s) 63, 711
RsaNI GTAC 2 cut(s) 62, 710
SaqAI TTAA 3 cut(s) 11, 588, 675
SatI GCNGC 1 cut(s) 555
Sau3AI GATC 2 cut(s) 396, 762
Sau96I GGNCC 2 cut(s) 45, 647
ScrFI CCNGG 3 cut(s) 294, 645, 726
SduI GDGCHC 1 cut(s) 642
SfaNI GCATC 1 cut(s) 505
SinI GGWCC 1 cut(s) 45
SphI GCATGC 1 cut(s) 185
Sse9I AATT 3 cut(s) 105, 405, 599
SspMI CTAG 1 cut(s) 758
StyD4I CCNGG 3 cut(s) 292, 643, 724
TasI AATT 3 cut(s) 105, 405, 599
TatI WGTACW 1 cut(s) 709
TfiI GAWTC 2 cut(s) 226, 778
Tru1I TTAA 3 cut(s) 11, 588, 675
Tru9I TTAA 3 cut(s) 11, 588, 675
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 1 cut(s) 554
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 3 cut(s) 132, 671, 782
TspRI CASTG 1 cut(s) 313
VpaK11BI GGWCC 1 cut(s) 45
XceI RCATGY 2 cut(s) 185, 242
XspI CTAG 1 cut(s) 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.