Rh6DG283300

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0020582
Physical Location & Seq
Forward (+)
47730009 .. 47746463
16455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG283300.1

Sequence Viewer

Length: 591 bp
ATGATGTTACTAATGAGCCATCATTCAACCACTTTAGGGATTGGGGAGGAAAAGCACCCTCCTTCTGTAATTGTCAAGAAGATACTGGTGGGAAACAAGGCTGACATCGGTGAAAGCAAAAGGGCAGTGACGAAATCTAGAGGTCAGGCTCTTGCTGATGAGTTCGGCCTCAAGTTCTTCGAAACCAGTGCAAAGACGAACATGAATGTGGAAGGGGTTTTCCTTGCGTTAGCCAGGGATATCAGGCCAGAATTGAACGCTTCTAGGAGCATTCACGAGCGTGCGGCACTTGGGATCAACCGATTAGCTGCACAGAGGACTTGGAAGAGCCACTCAAACTTTTCTTCACACTCCTGCTGCTCAGAGGTTCTTGCTCTGCTAGTAAAAATGAGGATATCTCCTCACTTGCTTATAGAATTTCTTTTCTCTATCACTGGGGCAGATTACCATTGCAGAATAAACAATGTGGGCACTAGAATTGAATTTCTAGTTTCCATTTTAATTTGCCAACTTTTGGAACTGGATTCTCTTCTGATGAAAGCTTATCTGGTTGTTATGCAATGCTGCCAGCTGCAACTGAAGGACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04152 ko04530 ko04972 map04144 map04152 map04530 map04972
Pfam Domains
Protein Families
Ras

Protein Analysis

196

Amino Acids

21.88

Weight (kDa)

9.1

Isoelectric Point (pI)

30.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 25 - 82 2.6e-14 Ras family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020582)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0285671
rosa_multiflora Rmu_sc0001989.1_g000010
rosa_roxburghii Rroxscaffold_7G00182840
rosa_samantha Rh6BG289600 Rh6CG290600 Rh6DG283300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 514
AciI CCGC 1 cut(s) 284
AclWI GGATC 1 cut(s) 302
AcsI RAATTY 2 cut(s) 416, 482
AfiI CCNNNNNNNGG 2 cut(s) 36, 514
AgsI TTSAA 3 cut(s) 27, 256, 482
AjnI CCWGG 1 cut(s) 233
AleI CACNNNNGTG 1 cut(s) 279
AluBI AGCT 3 cut(s) 308, 542, 571
AluI AGCT 3 cut(s) 308, 542, 571
AlwI GGATC 1 cut(s) 302
AoxI GGCC 2 cut(s) 166, 245
ApeKI GCWGC 4 cut(s) 308, 357, 564, 571
ApoI RAATTY 2 cut(s) 416, 482
AsuHPI GGTGA 1 cut(s) 122
AsuII TTCGAA 1 cut(s) 180
BaeGI GKGCMC 1 cut(s) 473
BauI CACGAG 1 cut(s) 275
BbvI GCAGC 4 cut(s) 295, 344, 551, 558
BccI CCATC 1 cut(s) 27
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 1 cut(s) 235
BfaI CTAG 5 cut(s) 138, 264, 380, 474, 488
BisI GCNGC 5 cut(s) 285, 309, 358, 565, 572
BlsI GCNGC 5 cut(s) 286, 310, 359, 566, 573
Bme1390I CCNGG 1 cut(s) 235
BmrFI CCNGG 1 cut(s) 235
BmrI ACTGGG 1 cut(s) 444
BmuI ACTGGG 1 cut(s) 444
BplI GAGNNNNNCTC 2 cut(s) 382, 414
Bpu14I TTCGAA 1 cut(s) 180
BpuEI CTTGAG 1 cut(s) 155
BsaJI CCNNGG 1 cut(s) 234
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 514
Bse1I ACTGG 4 cut(s) 90, 186, 439, 525
Bse3DI GCAATG 2 cut(s) 448, 566
BseBI CCWGG 1 cut(s) 235
BseDI CCNNGG 1 cut(s) 234
BseLI CCNNNNNNNGG 2 cut(s) 36, 514
BseMI GCAATG 2 cut(s) 448, 566
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 4 cut(s) 90, 186, 439, 525
BseRI GAGGAG 1 cut(s) 390
BseSI GKGCMC 1 cut(s) 473
BseXI GCAGC 4 cut(s) 295, 344, 551, 558
BsgI GTGCAG 1 cut(s) 294
BshFI GGCC 2 cut(s) 168, 247
BslI CCNNNNNNNGG 2 cut(s) 36, 514
BsmI GAATGC 1 cut(s) 270
BsnI GGCC 2 cut(s) 168, 247
Bsp119I TTCGAA 1 cut(s) 180
Bsp1286I GDGCHC 1 cut(s) 473
Bsp143I GATC 1 cut(s) 294
BspACI CCGC 1 cut(s) 284
BspANI GGCC 2 cut(s) 168, 247
BspCNI CTCAG 1 cut(s) 374
BspPI GGATC 1 cut(s) 302
BspQI GCTCTTC 1 cut(s) 320
BspT104I TTCGAA 1 cut(s) 180
BsrDI GCAATG 2 cut(s) 448, 566
BsrI ACTGG 4 cut(s) 90, 186, 439, 525
BssECI CCNNGG 1 cut(s) 234
BssMI GATC 1 cut(s) 294
BssSI CACGAG 1 cut(s) 275
Bst2BI CACGAG 1 cut(s) 275
Bst2UI CCWGG 1 cut(s) 235
Bst6I CTCTTC 2 cut(s) 320, 534
BstBI TTCGAA 1 cut(s) 180
BstC8I GCNNGC 2 cut(s) 282, 569
BstDEI CTNAG 1 cut(s) 361
BstKTI GATC 1 cut(s) 297
BstMBI GATC 1 cut(s) 294
BstNI CCWGG 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 233
BstSLI GKGCMC 1 cut(s) 473
BstV1I GCAGC 4 cut(s) 295, 344, 551, 558
BsuRI GGCC 2 cut(s) 168, 247
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 3 cut(s) 132, 193, 432
Cac8I GCNNGC 2 cut(s) 282, 569
CviAII CATG 1 cut(s) 202
DdeI CTNAG 1 cut(s) 361
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
Eam1104I CTCTTC 2 cut(s) 320, 534
EarI CTCTTC 2 cut(s) 320, 534
Eco32I GATATC 2 cut(s) 241, 396
EcoRII CCWGG 1 cut(s) 233
EcoRV GATATC 2 cut(s) 241, 396
FaeI CATG 1 cut(s) 205
FaiI YATR 3 cut(s) 203, 413, 557
FatI CATG 1 cut(s) 201
Fnu4HI GCNGC 5 cut(s) 285, 309, 358, 565, 572
Fsp4HI GCNGC 5 cut(s) 285, 309, 358, 565, 572
FspBI CTAG 5 cut(s) 138, 264, 380, 474, 488
GluI GCNGC 5 cut(s) 285, 309, 358, 565, 572
HaeIII GGCC 2 cut(s) 168, 247
Hin1II CATG 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 540
HinfI GANTC 1 cut(s) 524
HphI GGTGA 1 cut(s) 122
Hpy188I TCNGA 2 cut(s) 364, 534
Hpy188III TCNNGA 3 cut(s) 76, 138, 275
HpyAV CCTTC 3 cut(s) 72, 206, 574
HpyCH4V TGCA 5 cut(s) 191, 311, 453, 559, 574
HpyF3I CTNAG 1 cut(s) 361
Hsp92II CATG 1 cut(s) 205
Kzo9I GATC 1 cut(s) 294
LguI GCTCTTC 1 cut(s) 320
LmnI GCTCC 1 cut(s) 267
Lsp1109I GCAGC 4 cut(s) 295, 344, 551, 558
MaeI CTAG 5 cut(s) 138, 264, 380, 474, 488
MaeIII GTNAC 2 cut(s) 6, 127
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MboII GAAGA 5 cut(s) 91, 169, 336, 337, 521
MhlI GDGCHC 1 cut(s) 473
MluCI AATT 6 cut(s) 69, 251, 416, 477, 482, 501
MnlI CCTC 8 cut(s) 40, 69, 134, 179, 309, 358, 384, 411
MseI TTAA 1 cut(s) 500
MslI CAYNNNNRTG 2 cut(s) 206, 279
MspA1I CMGCKG 1 cut(s) 571
MspR9I CCNGG 1 cut(s) 235
Mva1269I GAATGC 1 cut(s) 270
MvaI CCWGG 1 cut(s) 235
NdeII GATC 1 cut(s) 294
NlaIII CATG 1 cut(s) 205
NmuCI GTSAC 1 cut(s) 127
NspV TTCGAA 1 cut(s) 180
OliI CACNNNNGTG 1 cut(s) 279
PciSI GCTCTTC 1 cut(s) 320
PctI GAATGC 1 cut(s) 270
PfeI GAWTC 1 cut(s) 524
PflMI CCANNNNNTGG 1 cut(s) 514
PkrI GCNGC 5 cut(s) 286, 310, 359, 566, 573
Psp6I CCWGG 1 cut(s) 233
PspGI CCWGG 1 cut(s) 233
PvuII CAGCTG 1 cut(s) 571
RseI CAYNNNNRTG 2 cut(s) 206, 279
SapI GCTCTTC 1 cut(s) 320
SaqAI TTAA 1 cut(s) 500
SatI GCNGC 5 cut(s) 285, 309, 358, 565, 572
Sau3AI GATC 1 cut(s) 294
ScrFI CCNGG 1 cut(s) 235
SduI GDGCHC 1 cut(s) 473
SetI ASST 5 cut(s) 145, 310, 369, 544, 573
SfuI TTCGAA 1 cut(s) 180
SmiMI CAYNNNNRTG 2 cut(s) 206, 279
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 6 cut(s) 69, 251, 416, 477, 482, 501
SsiI CCGC 1 cut(s) 284
SspMI CTAG 5 cut(s) 138, 264, 380, 474, 488
StyD4I CCNGG 1 cut(s) 233
TaqI TCGA 1 cut(s) 180
TasI AATT 6 cut(s) 69, 251, 416, 477, 482, 501
TauI GCSGC 1 cut(s) 287
TfiI GAWTC 1 cut(s) 524
Tru1I TTAA 1 cut(s) 500
Tru9I TTAA 1 cut(s) 500
TscAI CASTG 3 cut(s) 132, 193, 439
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 4 cut(s) 308, 357, 564, 571
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 2 cut(s) 218, 551
TspRI CASTG 3 cut(s) 132, 193, 439
Van91I CCANNNNNTGG 1 cut(s) 514
XapI RAATTY 2 cut(s) 416, 482
XbaI TCTAGA 1 cut(s) 137
XspI CTAG 5 cut(s) 138, 264, 380, 474, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.