Rh7AG111600

Belongs to the MAK16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0025564
Physical Location & Seq
Forward (+)
8748404 .. 8749349
946 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG111600.1

Sequence Viewer

Length: 501 bp
ATGTATTGGCCTAAGTTTCTTGTACATAAAACAAAGCAACGGCTGACGAAAATGACTCAGATGCGGATACGCATGAGGAAGCTTGCTTTGAAAACGAGGGAGAAGATAATGACAATACCAAGGAAAGAGATAAAAAGAGAGGCCAGAAGAGAGGAAAAGGCTGAAAAAGCTGCGGTGTTGGATAAGAGCATCGAGAAAGAGCTATTAAGCCGCCTTAAGGAAGGAATGTATGGTGATATATATAACTATCCCTTTGATAAATTCCAAAAACTCATTGAAGGGGAAGAAATGGAGGTGGATACTGAAAGAGAACAAGAAGATGAGGAGGAGGCTGAAATTGAATATGTTGAAGGTTATGATGAACTTGAAGAGGAAGATGATATTGAAGATCTTGGTGGTTTTGCAATGGACCATTCTCAGGCAGACGATGATAATGGTAGAGCTAAATTCTATTATCTTAACATAATGCAGATGATCATGATGATATTCCCTTTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mak16 Ribosomal_L28e

Protein Analysis

166

Amino Acids

20.01

Weight (kDa)

4.87

Isoelectric Point (pI)

57.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mak16 PF04874 41 - 130 4.2e-23 Mak16 protein C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025564)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003554.1_g000001
rosa_samantha Rh7AG111600 Rh7DG115500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 64, 173, 211
AcsI RAATTY 2 cut(s) 260, 446
AfaI GTAC 1 cut(s) 24
AfiI CCNNNNNNNGG 2 cut(s) 217, 418
AflII CTTAAG 1 cut(s) 215
AgsI TTSAA 6 cut(s) 91, 278, 341, 350, 368, 386
AluBI AGCT 4 cut(s) 82, 170, 202, 443
AluI AGCT 4 cut(s) 82, 170, 202, 443
AoxI GGCC 2 cut(s) 8, 141
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 2 cut(s) 260, 446
AspS9I GGNCC 1 cut(s) 409
AsuHPI GGTGA 1 cut(s) 245
AvaII GGWCC 1 cut(s) 409
BbvI GCAGC 1 cut(s) 157
BceAI ACGGC 1 cut(s) 56
BciVI GTATCC 2 cut(s) 60, 292
BclI TGATCA 1 cut(s) 474
BfrI CTTAAG 1 cut(s) 215
BfuI GTATCC 2 cut(s) 60, 292
BglII AGATCT 1 cut(s) 388
BisI GCNGC 2 cut(s) 171, 211
BlsI GCNGC 2 cut(s) 172, 212
Bme18I GGWCC 1 cut(s) 409
BmgT120I GGNCC 1 cut(s) 409
BmsI GCATC 2 cut(s) 51, 198
BsaJI CCNNGG 1 cut(s) 119
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 418
Bse3DI GCAATG 1 cut(s) 411
BseDI CCNNGG 1 cut(s) 119
BseLI CCNNNNNNNGG 2 cut(s) 217, 418
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 2 cut(s) 71, 431
BseRI GAGGAG 2 cut(s) 338, 341
BseXI GCAGC 1 cut(s) 157
BshFI GGCC 2 cut(s) 10, 143
BslI CCNNNNNNNGG 2 cut(s) 217, 418
BsnI GGCC 2 cut(s) 10, 143
Bsp1407I TGTACA 1 cut(s) 22
Bsp143I GATC 2 cut(s) 388, 474
BspACI CCGC 3 cut(s) 64, 173, 211
BspANI GGCC 2 cut(s) 10, 143
BspCNI CTCAG 2 cut(s) 70, 430
BspHI TCATGA 1 cut(s) 477
BspTI CTTAAG 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 411
BsrGI TGTACA 1 cut(s) 22
BssECI CCNNGG 1 cut(s) 119
BssMI GATC 2 cut(s) 388, 474
BssT1I CCWWGG 1 cut(s) 119
Bst6I CTCTTC 2 cut(s) 142, 363
BstAFI CTTAAG 1 cut(s) 215
BstAUI TGTACA 1 cut(s) 22
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 3 cut(s) 12, 57, 417
BstKTI GATC 2 cut(s) 391, 477
BstMBI GATC 2 cut(s) 388, 474
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstV1I GCAGC 1 cut(s) 157
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
BsuI GTATCC 2 cut(s) 60, 292
BsuRI GGCC 2 cut(s) 10, 143
Cac8I GCNNGC 1 cut(s) 84
CciI TCATGA 1 cut(s) 477
Cfr13I GGNCC 1 cut(s) 409
Csp6I GTAC 1 cut(s) 23
CviAII CATG 2 cut(s) 73, 478
CviQI GTAC 1 cut(s) 23
DdeI CTNAG 3 cut(s) 12, 57, 417
DpnI GATC 2 cut(s) 390, 476
DpnII GATC 2 cut(s) 388, 474
Eam1104I CTCTTC 2 cut(s) 142, 363
EarI CTCTTC 2 cut(s) 142, 363
Eco130I CCWWGG 1 cut(s) 119
Eco47I GGWCC 1 cut(s) 409
EcoT14I CCWWGG 1 cut(s) 119
ErhI CCWWGG 1 cut(s) 119
FaeI CATG 2 cut(s) 76, 481
FatI CATG 2 cut(s) 72, 477
FbaI TGATCA 1 cut(s) 474
Fnu4HI GCNGC 2 cut(s) 171, 211
Fsp4HI GCNGC 2 cut(s) 171, 211
GluI GCNGC 2 cut(s) 171, 211
HaeIII GGCC 2 cut(s) 10, 143
Hin1II CATG 2 cut(s) 76, 481
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 1 cut(s) 55
HphI GGTGA 1 cut(s) 245
Hpy188I TCNGA 1 cut(s) 60
Hpy188III TCNNGA 2 cut(s) 193, 478
HpyAV CCTTC 3 cut(s) 215, 272, 344
HpyCH4V TGCA 3 cut(s) 404, 469, 497
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpyF3I CTNAG 3 cut(s) 12, 57, 417
Hsp92II CATG 2 cut(s) 76, 481
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 2 cut(s) 388, 474
LpnPI CCDG 2 cut(s) 157, 404
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 2 cut(s) 51, 198
MalI GATC 2 cut(s) 390, 476
MboI GATC 2 cut(s) 388, 474
MboII GAAGA 7 cut(s) 115, 159, 296, 329, 380, 386, 398
MflI RGATCY 1 cut(s) 388
MluCI AATT 3 cut(s) 260, 336, 446
MlyI GAGTC 1 cut(s) 49
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 9 cut(s) 69, 90, 133, 145, 286, 316, 319, 322, 364
MseI TTAA 3 cut(s) 206, 216, 459
MspCI CTTAAG 1 cut(s) 215
MwoI GCNNNNNNNGC 1 cut(s) 167
NdeII GATC 2 cut(s) 388, 474
NlaIII CATG 2 cut(s) 76, 481
PagI TCATGA 1 cut(s) 477
PkrI GCNGC 2 cut(s) 172, 212
PleI GAGTC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 49
PspPI GGNCC 1 cut(s) 409
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
SaqAI TTAA 3 cut(s) 206, 216, 459
SatI GCNGC 2 cut(s) 171, 211
Sau3AI GATC 2 cut(s) 388, 474
Sau96I GGNCC 1 cut(s) 409
SchI GAGTC 1 cut(s) 49
SetI ASST 6 cut(s) 84, 172, 204, 297, 355, 445
SfaNI GCATC 2 cut(s) 51, 198
SinI GGWCC 1 cut(s) 409
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 3 cut(s) 260, 336, 446
SsiI CCGC 3 cut(s) 64, 173, 211
StyI CCWWGG 1 cut(s) 119
TaqI TCGA 1 cut(s) 192
TasI AATT 3 cut(s) 260, 336, 446
TatI WGTACW 1 cut(s) 22
TauI GCSGC 1 cut(s) 213
Tru1I TTAA 3 cut(s) 206, 216, 459
Tru9I TTAA 3 cut(s) 206, 216, 459
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 1 cut(s) 375
Vha464I CTTAAG 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 409
XapI RAATTY 2 cut(s) 260, 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.