Rh7DG115500

Belongs to the MAK16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0025564
Physical Location & Seq
Forward (+)
9785883 .. 9787601
1719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG115500.1

Sequence Viewer

Length: 600 bp
ATGTATTGGCCTAAGTTTCTTGTACATAAAACAAAGCAACGGCTGACTAAAATGACTCAGATGCGGATACGCATGAGGAAGCTTGCTTTGAAAACGAGGGAGAAGATAATGACAATACCAAGGAAAGAGATAAAAAGAGAGGCCAGAAGAGAGGAAAAGGCTGAAAAAGCTGCGGTGTTGGATAAGAGCATCGAGAAAGAACTATTAAGCCGCCTTAAGGAAGGAATGTATGGTGATATATATAACTATCCCTTTGATAAATTCCAAAAACTCATTGAAGGGGAAGAAATGGAGGTGGATACTGAAAGAGAACAAGAAGATGAGGAGGAGGCTGAAATTGAATATGTTGAAGGTTATGATGAACTTGAAGAGGAAGATGATATTGAAGATCTTGGTGGTTTTGCAATGGACCATTCTCATGCAGACGATGATAGTGGTGGAGATGAAGAAGCAGAAGCAGTTACTCGCAAGAGAGAGAGAAAAGAATCTGATTCTAGAAAGTTTGGAAAAGATAAAGGTGTTGCCAAGTTGAAGAAGCCTAGAGTATTGATTGAGGTTGAATATGAAGATACCCGCGAAAGACAGAAGGCAGTTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mak16 Ribosomal_L28e

Protein Analysis

199

Amino Acids

23.72

Weight (kDa)

5.14

Isoelectric Point (pI)

50.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mak16 PF04874 41 - 130 6.6e-23 Mak16 protein C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025564)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003554.1_g000001
rosa_samantha Rh7AG111600 Rh7DG115500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 576
AciI CCGC 4 cut(s) 64, 173, 211, 574
AcsI RAATTY 1 cut(s) 260
AfaI GTAC 1 cut(s) 24
AfiI CCNNNNNNNGG 1 cut(s) 217
AflII CTTAAG 1 cut(s) 215
AgsI TTSAA 8 cut(s) 91, 278, 341, 350, 368, 386, 532, 560
AluBI AGCT 2 cut(s) 82, 170
AluI AGCT 2 cut(s) 82, 170
AoxI GGCC 2 cut(s) 8, 141
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 1 cut(s) 260
AspS9I GGNCC 1 cut(s) 409
AsuHPI GGTGA 1 cut(s) 245
AvaII GGWCC 1 cut(s) 409
BbvI GCAGC 1 cut(s) 157
BceAI ACGGC 1 cut(s) 56
BciVI GTATCC 2 cut(s) 60, 292
BfaI CTAG 2 cut(s) 495, 540
BfrI CTTAAG 1 cut(s) 215
BfuI GTATCC 2 cut(s) 60, 292
BglII AGATCT 1 cut(s) 388
BisI GCNGC 2 cut(s) 171, 211
BlsI GCNGC 2 cut(s) 172, 212
Bme18I GGWCC 1 cut(s) 409
BmgT120I GGNCC 1 cut(s) 409
BmsI GCATC 2 cut(s) 51, 198
BsaJI CCNNGG 1 cut(s) 119
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse3DI GCAATG 1 cut(s) 411
BseDI CCNNGG 1 cut(s) 119
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 1 cut(s) 71
BseRI GAGGAG 2 cut(s) 338, 341
BseXI GCAGC 1 cut(s) 157
Bsh1236I CGCG 1 cut(s) 576
BshFI GGCC 2 cut(s) 10, 143
BslI CCNNNNNNNGG 1 cut(s) 217
BsnI GGCC 2 cut(s) 10, 143
Bsp1407I TGTACA 1 cut(s) 22
Bsp143I GATC 1 cut(s) 388
BspACI CCGC 4 cut(s) 64, 173, 211, 574
BspANI GGCC 2 cut(s) 10, 143
BspCNI CTCAG 1 cut(s) 70
BspFNI CGCG 1 cut(s) 576
BspTI CTTAAG 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 411
BsrGI TGTACA 1 cut(s) 22
BssECI CCNNGG 1 cut(s) 119
BssMI GATC 1 cut(s) 388
BssT1I CCWWGG 1 cut(s) 119
Bst6I CTCTTC 2 cut(s) 142, 363
BstAFI CTTAAG 1 cut(s) 215
BstAUI TGTACA 1 cut(s) 22
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 2 cut(s) 12, 57
BstFNI CGCG 1 cut(s) 576
BstKTI GATC 1 cut(s) 391
BstMBI GATC 1 cut(s) 388
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstUI CGCG 1 cut(s) 576
BstV1I GCAGC 1 cut(s) 157
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
BsuI GTATCC 2 cut(s) 60, 292
BsuRI GGCC 2 cut(s) 10, 143
Cac8I GCNNGC 1 cut(s) 84
Cfr13I GGNCC 1 cut(s) 409
Csp6I GTAC 1 cut(s) 23
CviAII CATG 2 cut(s) 73, 419
CviJI RGCY 9 cut(s) 10, 43, 82, 143, 161, 170, 210, 332, 538
CviKI_1 RGCY 9 cut(s) 10, 43, 82, 143, 161, 170, 210, 332, 538
CviQI GTAC 1 cut(s) 23
DdeI CTNAG 2 cut(s) 12, 57
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
Eam1104I CTCTTC 2 cut(s) 142, 363
EarI CTCTTC 2 cut(s) 142, 363
Eco130I CCWWGG 1 cut(s) 119
Eco47I GGWCC 1 cut(s) 409
EcoT14I CCWWGG 1 cut(s) 119
ErhI CCWWGG 1 cut(s) 119
FaeI CATG 2 cut(s) 76, 422
FatI CATG 2 cut(s) 72, 418
FauI CCCGC 1 cut(s) 581
Fnu4HI GCNGC 2 cut(s) 171, 211
Fsp4HI GCNGC 2 cut(s) 171, 211
FspBI CTAG 2 cut(s) 495, 540
GluI GCNGC 2 cut(s) 171, 211
HaeIII GGCC 2 cut(s) 10, 143
Hin1II CATG 2 cut(s) 76, 422
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 3 cut(s) 55, 485, 491
HphI GGTGA 1 cut(s) 245
Hpy188I TCNGA 2 cut(s) 60, 490
Hpy188III TCNNGA 2 cut(s) 193, 495
HpyAV CCTTC 4 cut(s) 215, 272, 344, 580
HpyCH4V TGCA 2 cut(s) 404, 422
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpyF3I CTNAG 2 cut(s) 12, 57
Hsp92II CATG 2 cut(s) 76, 422
Kzo9I GATC 1 cut(s) 388
LpnPI CCDG 1 cut(s) 157
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 2 cut(s) 51, 198
MaeI CTAG 2 cut(s) 495, 540
MaeIII GTNAC 1 cut(s) 460
MalI GATC 1 cut(s) 390
MboI GATC 1 cut(s) 388
MflI RGATCY 1 cut(s) 388
MluCI AATT 2 cut(s) 260, 336
MlyI GAGTC 1 cut(s) 49
MmeI TCCRAC 1 cut(s) 159
MseI TTAA 2 cut(s) 206, 216
MslI CAYNNNNRTG 1 cut(s) 417
MspCI CTTAAG 1 cut(s) 215
MvnI CGCG 1 cut(s) 576
MwoI GCNNNNNNNGC 1 cut(s) 167
NdeII GATC 1 cut(s) 388
NlaIII CATG 2 cut(s) 76, 422
PfeI GAWTC 2 cut(s) 485, 491
PkrI GCNGC 2 cut(s) 172, 212
PleI GAGTC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 49
PspPI GGNCC 1 cut(s) 409
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
RseI CAYNNNNRTG 1 cut(s) 417
SaqAI TTAA 2 cut(s) 206, 216
SatI GCNGC 2 cut(s) 171, 211
Sau3AI GATC 1 cut(s) 388
Sau96I GGNCC 1 cut(s) 409
SchI GAGTC 1 cut(s) 49
SetI ASST 6 cut(s) 84, 172, 297, 355, 520, 558
SfaNI GCATC 2 cut(s) 51, 198
SinI GGWCC 1 cut(s) 409
SmiMI CAYNNNNRTG 1 cut(s) 417
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 2 cut(s) 260, 336
SsiI CCGC 4 cut(s) 64, 173, 211, 574
SspMI CTAG 2 cut(s) 495, 540
StyI CCWWGG 1 cut(s) 119
TaqI TCGA 1 cut(s) 192
TasI AATT 2 cut(s) 260, 336
TatI WGTACW 1 cut(s) 22
TauI GCSGC 1 cut(s) 213
TfiI GAWTC 2 cut(s) 485, 491
Tru1I TTAA 2 cut(s) 206, 216
Tru9I TTAA 2 cut(s) 206, 216
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 3 cut(s) 375, 459, 579
Vha464I CTTAAG 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 409
XapI RAATTY 1 cut(s) 260
XbaI TCTAGA 1 cut(s) 494
XspI CTAG 2 cut(s) 495, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.