Rw0G002160

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00067
OG0020744
Physical Location & Seq
Forward (+)
2266 .. 8964
6699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G002160.1

Sequence Viewer

Length: 423 bp
ATGGAAGGTTGGCGTCAAGGCGGTGGCGGCGGAGCTGCCGGTGGCGGGACGATGGGATCTGTCCTCCGCTCCTTCAGGGGTGATGGCTGGTTCGATATTGATCTGGTTTACGGCGGAAGGGAAGATGGTATGATGCTGGCGGCGGGCCTTGCTGGGTTGGCCGGTGAGAAATGGGCTCTTCACCGGAGGATTGCAACCCAAGCCTTCACCGTGGAGAGAGTCAAGGGTTGGGTGCCTGAAATTGTGGCTAGTGCCACATACATGCTTGAGAAATGGGGGAGTTTGAAAGGGGAAAGAGATGAGTTTGAGTTGGATGTTCATGAAGAGCTTCATGAACTCTTGGCGGATATCATATCCAGGGTAGCCTTTGGAAGTAGTTTTGAGGAAGGGAAGCGTATATTAAATTACAAGAAAAACAGATGA

Protein Analysis

140

Amino Acids

15.39

Weight (kDa)

5.65

Isoelectric Point (pI)

38.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 46 - 131 7.4e-10 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020744)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004226.1_g000008 Rmu_sc0014374.1_g000006
rosa_rugosa Rorug06G0298400
rosa_samantha Rh6AG411100
rosa_wichuraiana Rw0G002160 Rw6G035970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 232
AccBSI CCGCTC 1 cut(s) 69
AciI CCGC 9 cut(s) 21, 27, 30, 45, 67, 114, 140, 143, 344
AclWI GGATC 1 cut(s) 64
AcoI YGGCCR 1 cut(s) 159
AcuI CTGAAG 1 cut(s) 58
AcyI GRCGYC 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 45
AgsI TTSAA 1 cut(s) 286
AjnI CCWGG 1 cut(s) 356
AluBI AGCT 2 cut(s) 35, 328
AluI AGCT 2 cut(s) 35, 328
AlwI GGATC 1 cut(s) 64
AoxI GGCC 2 cut(s) 145, 159
ApeKI GCWGC 1 cut(s) 35
Asp700I GAANNNNTTC 1 cut(s) 327
AspS9I GGNCC 1 cut(s) 145
AsuHPI GGTGA 4 cut(s) 92, 173, 176, 199
BanI GGYRCC 1 cut(s) 232
BanII GRGCYC 1 cut(s) 178
BbvI GCAGC 1 cut(s) 22
BccI CCATC 3 cut(s) 46, 77, 119
BceAI ACGGC 1 cut(s) 127
BciT130I CCWGG 1 cut(s) 358
BfaI CTAG 1 cut(s) 249
BisI GCNGC 3 cut(s) 28, 36, 141
BlsI GCNGC 3 cut(s) 29, 37, 142
Bme1390I CCNGG 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 358
BmsI GCATC 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 287
BsaBI GATNNNNATC 1 cut(s) 99
BsaHI GRCGYC 1 cut(s) 13
BsaJI CCNNGG 2 cut(s) 210, 357
BsaWI WCCGGW 1 cut(s) 183
Bsc4I CCNNNNNNNGG 1 cut(s) 45
Bse118I RCCGGY 2 cut(s) 38, 161
Bse8I GATNNNNATC 1 cut(s) 99
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 2 cut(s) 210, 357
BseGI GGATG 1 cut(s) 319
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 45
BseXI GCAGC 1 cut(s) 22
BseYI CCCAGC 1 cut(s) 152
BshFI GGCC 2 cut(s) 147, 161
BshNI GGYRCC 1 cut(s) 232
BsiSI CCGG 3 cut(s) 39, 162, 184
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 61
BsnI GGCC 2 cut(s) 147, 161
Bsp1286I GDGCHC 1 cut(s) 178
Bsp143I GATC 2 cut(s) 56, 100
BspACI CCGC 9 cut(s) 21, 27, 30, 45, 67, 114, 140, 143, 344
BspANI GGCC 2 cut(s) 147, 161
BspHI TCATGA 2 cut(s) 319, 331
BspLI GGNNCC 1 cut(s) 234
BspPI GGATC 1 cut(s) 64
BspQI GCTCTTC 2 cut(s) 183, 318
BspT107I GGYRCC 1 cut(s) 232
BsrBI CCGCTC 1 cut(s) 69
BsrFI RCCGGY 2 cut(s) 38, 161
BssAI RCCGGY 2 cut(s) 38, 161
BssECI CCNNGG 2 cut(s) 210, 357
BssMI GATC 2 cut(s) 56, 100
BssNI GRCGYC 1 cut(s) 13
Bst2UI CCWGG 1 cut(s) 358
Bst4CI ACNGT 1 cut(s) 211
Bst6I CTCTTC 2 cut(s) 183, 318
BstACI GRCGYC 1 cut(s) 13
BstC8I GCNNGC 2 cut(s) 138, 145
BstDSI CCRYGG 1 cut(s) 210
BstF5I GGATG 1 cut(s) 319
BstKTI GATC 2 cut(s) 59, 103
BstMBI GATC 2 cut(s) 56, 100
BstMWI GCNNNNNNNGC 4 cut(s) 27, 149, 158, 200
BstNI CCWGG 1 cut(s) 358
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 1 cut(s) 356
BstV1I GCAGC 1 cut(s) 22
BstX2I RGATCY 1 cut(s) 56
BstYI RGATCY 1 cut(s) 56
BsuRI GGCC 2 cut(s) 147, 161
BtgI CCRYGG 1 cut(s) 210
BtsCI GGATG 1 cut(s) 319
Cac8I GCNNGC 2 cut(s) 138, 145
CciI TCATGA 2 cut(s) 319, 331
Cfr10I RCCGGY 2 cut(s) 38, 161
Cfr13I GGNCC 1 cut(s) 145
CseI GACGC 1 cut(s) 2
CviAII CATG 3 cut(s) 262, 320, 332
CviJI RGCY 9 cut(s) 35, 87, 147, 161, 176, 203, 248, 328, 365
CviKI_1 RGCY 9 cut(s) 35, 87, 147, 161, 176, 203, 248, 328, 365
DpnI GATC 2 cut(s) 58, 102
DpnII GATC 2 cut(s) 56, 100
EaeI YGGCCR 1 cut(s) 159
Eam1104I CTCTTC 2 cut(s) 183, 318
EarI CTCTTC 2 cut(s) 183, 318
EciI GGCGGA 3 cut(s) 45, 129, 359
Eco24I GRGCYC 1 cut(s) 178
Eco32I GATATC 1 cut(s) 349
Eco57I CTGAAG 1 cut(s) 58
EcoRII CCWGG 1 cut(s) 356
EcoRV GATATC 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 178
FaeI CATG 3 cut(s) 265, 323, 335
FaiI YATR 7 cut(s) 131, 259, 263, 321, 333, 353, 398
FaqI GGGAC 1 cut(s) 61
FatI CATG 3 cut(s) 261, 319, 331
FauI CCCGC 2 cut(s) 38, 136
Fnu4HI GCNGC 3 cut(s) 28, 36, 141
FokI GGATG 1 cut(s) 326
FriOI GRGCYC 1 cut(s) 178
Fsp4HI GCNGC 3 cut(s) 28, 36, 141
FspBI CTAG 1 cut(s) 249
GluI GCNGC 3 cut(s) 28, 36, 141
GsaI CCCAGC 1 cut(s) 156
HaeIII GGCC 2 cut(s) 147, 161
HapII CCGG 3 cut(s) 39, 162, 184
HgaI GACGC 1 cut(s) 2
Hin1I GRCGYC 1 cut(s) 13
Hin1II CATG 3 cut(s) 265, 323, 335
HinfI GANTC 1 cut(s) 219
HpaII CCGG 3 cut(s) 39, 162, 184
HphI GGTGA 4 cut(s) 92, 173, 176, 199
Hpy166II GTNNAC 1 cut(s) 109
Hpy188III TCNNGA 2 cut(s) 320, 332
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 4 cut(s) 82, 111, 214, 380
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4V TGCA 1 cut(s) 194
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 149, 158, 200
Hsp92I GRCGYC 1 cut(s) 13
Hsp92II CATG 3 cut(s) 265, 323, 335
Kzo9I GATC 2 cut(s) 56, 100
LguI GCTCTTC 2 cut(s) 183, 318
LmnI GCTCC 2 cut(s) 32, 74
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 1 cut(s) 123
MaeI CTAG 1 cut(s) 249
MalI GATC 2 cut(s) 58, 102
MbiI CCGCTC 1 cut(s) 69
MboI GATC 2 cut(s) 56, 100
MboII GAAGA 3 cut(s) 134, 170, 335
MflI RGATCY 1 cut(s) 56
MhlI GDGCHC 1 cut(s) 178
MluCI AATT 2 cut(s) 240, 403
MlyI GAGTC 1 cut(s) 228
MmeI TCCRAC 1 cut(s) 291
MnlI CCTC 3 cut(s) 74, 180, 376
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 1 cut(s) 401
MslI CAYNNNNRTG 1 cut(s) 260
MspI CCGG 3 cut(s) 39, 162, 184
MspR9I CCNGG 1 cut(s) 358
MvaI CCWGG 1 cut(s) 358
MwoI GCNNNNNNNGC 4 cut(s) 27, 149, 158, 200
NdeII GATC 2 cut(s) 56, 100
NlaIII CATG 3 cut(s) 265, 323, 335
NlaIV GGNNCC 1 cut(s) 234
NspI RCATGY 1 cut(s) 265
PagI TCATGA 2 cut(s) 319, 331
PciSI GCTCTTC 2 cut(s) 183, 318
PdmI GAANNNNTTC 1 cut(s) 327
PkrI GCNGC 3 cut(s) 29, 37, 142
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
Psp6I CCWGG 1 cut(s) 356
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 1 cut(s) 356
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 1 cut(s) 145
PsuI RGATCY 1 cut(s) 56
RseI CAYNNNNRTG 1 cut(s) 260
SapI GCTCTTC 2 cut(s) 183, 318
SaqAI TTAA 1 cut(s) 401
SatI GCNGC 3 cut(s) 28, 36, 141
Sau3AI GATC 2 cut(s) 56, 100
Sau96I GGNCC 1 cut(s) 145
SchI GAGTC 1 cut(s) 228
ScrFI CCNGG 1 cut(s) 358
SduI GDGCHC 1 cut(s) 178
SetI ASST 3 cut(s) 10, 37, 330
SfaNI GCATC 1 cut(s) 123
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 2 cut(s) 240, 403
SsiI CCGC 9 cut(s) 21, 27, 30, 45, 67, 114, 140, 143, 344
SspMI CTAG 1 cut(s) 249
StyD4I CCNGG 1 cut(s) 356
TaaI ACNGT 1 cut(s) 211
TaqI TCGA 1 cut(s) 93
TasI AATT 2 cut(s) 240, 403
TauI GCSGC 2 cut(s) 30, 143
Tru1I TTAA 1 cut(s) 401
Tru9I TTAA 1 cut(s) 401
TseI GCWGC 1 cut(s) 35
TspDTI ATGAA 4 cut(s) 308, 320, 336, 348
XceI RCATGY 1 cut(s) 265
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.