Rw6G035970

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0020744
Physical Location & Seq
Reverse (-)
60555169 .. 60555882
714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G035970.1

Sequence Viewer

Length: 291 bp
ATGTTTGCTCAGGGCCTTGCTGGGTTGGCCGGTGAGAAATGGGCTCTTCACCGGAGGATTGCAACCCAAGCCTTCACCGTGGAGAGAGTCAAGGGTTGGGTGCCTGAAATTGTGGCTAGTGCCACATACATGCTTGAGAAATGGGGGAGTTTGAAAGGGGAAAGAGATGAGTTTGAGTTGGATGTTCATGAAGAGCTTCATGAACTCTTGGCGGATATCATATCCAGGGTAGCCTTTGGAAGTAGTTTTGAGGAAGGGAAGCGTATATTAAATTACAAGAAAAACAGATGA

Protein Analysis

96

Amino Acids

10.98

Weight (kDa)

6.29

Isoelectric Point (pI)

32.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 4 - 87 2.5e-10 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020744)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004226.1_g000008 Rmu_sc0014374.1_g000006
rosa_rugosa Rorug06G0298400
rosa_samantha Rh6AG411100
rosa_wichuraiana Rw0G002160 Rw6G035970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 100
AciI CCGC 1 cut(s) 212
AcoI YGGCCR 1 cut(s) 27
AgsI TTSAA 1 cut(s) 154
AjnI CCWGG 1 cut(s) 224
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
AoxI GGCC 2 cut(s) 13, 27
Asp700I GAANNNNTTC 1 cut(s) 195
AspS9I GGNCC 1 cut(s) 13
AsuHPI GGTGA 3 cut(s) 41, 44, 67
BanI GGYRCC 1 cut(s) 100
BanII GRGCYC 1 cut(s) 46
BciT130I CCWGG 1 cut(s) 226
BfaI CTAG 1 cut(s) 117
Bme1390I CCNGG 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 13
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 1 cut(s) 226
Bpu10I CCTNAGC 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 155
BsaJI CCNNGG 2 cut(s) 78, 225
BsaWI WCCGGW 1 cut(s) 51
Bse118I RCCGGY 1 cut(s) 29
BseBI CCWGG 1 cut(s) 226
BseDI CCNNGG 2 cut(s) 78, 225
BseGI GGATG 1 cut(s) 187
BseMII CTCAG 1 cut(s) 23
BseYI CCCAGC 1 cut(s) 20
BshFI GGCC 2 cut(s) 15, 29
BshNI GGYRCC 1 cut(s) 100
BsiSI CCGG 2 cut(s) 30, 52
BsnI GGCC 2 cut(s) 15, 29
Bsp1286I GDGCHC 1 cut(s) 46
BspACI CCGC 1 cut(s) 212
BspANI GGCC 2 cut(s) 15, 29
BspCNI CTCAG 1 cut(s) 22
BspHI TCATGA 2 cut(s) 187, 199
BspLI GGNNCC 1 cut(s) 102
BspQI GCTCTTC 2 cut(s) 51, 186
BspT107I GGYRCC 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 29
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 2 cut(s) 78, 225
Bst2UI CCWGG 1 cut(s) 226
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 2 cut(s) 51, 186
BstDEI CTNAG 1 cut(s) 9
BstDSI CCRYGG 1 cut(s) 78
BstF5I GGATG 1 cut(s) 187
BstMWI GCNNNNNNNGC 2 cut(s) 26, 68
BstNI CCWGG 1 cut(s) 226
BstNSI RCATGY 1 cut(s) 133
BstSCI CCNGG 1 cut(s) 224
BsuRI GGCC 2 cut(s) 15, 29
BtgI CCRYGG 1 cut(s) 78
BtsCI GGATG 1 cut(s) 187
CciI TCATGA 2 cut(s) 187, 199
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 1 cut(s) 13
CviAII CATG 3 cut(s) 130, 188, 200
CviJI RGCY 7 cut(s) 15, 29, 44, 71, 116, 196, 233
CviKI_1 RGCY 7 cut(s) 15, 29, 44, 71, 116, 196, 233
DdeI CTNAG 1 cut(s) 9
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 2 cut(s) 51, 186
EarI CTCTTC 2 cut(s) 51, 186
EciI GGCGGA 1 cut(s) 227
Eco24I GRGCYC 1 cut(s) 46
Eco32I GATATC 1 cut(s) 217
EcoO109I RGGNCCY 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 224
EcoRV GATATC 1 cut(s) 217
EcoT38I GRGCYC 1 cut(s) 46
FaeI CATG 3 cut(s) 133, 191, 203
FaiI YATR 6 cut(s) 127, 131, 189, 201, 221, 266
FatI CATG 3 cut(s) 129, 187, 199
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 1 cut(s) 46
FspBI CTAG 1 cut(s) 117
GsaI CCCAGC 1 cut(s) 24
HaeIII GGCC 2 cut(s) 15, 29
HapII CCGG 2 cut(s) 30, 52
Hin1II CATG 3 cut(s) 133, 191, 203
HinfI GANTC 1 cut(s) 87
HpaII CCGG 2 cut(s) 30, 52
HphI GGTGA 3 cut(s) 41, 44, 67
Hpy188III TCNNGA 2 cut(s) 188, 200
HpyAV CCTTC 2 cut(s) 82, 248
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4V TGCA 1 cut(s) 62
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 68
HpyF3I CTNAG 1 cut(s) 9
Hsp92II CATG 3 cut(s) 133, 191, 203
LguI GCTCTTC 2 cut(s) 51, 186
LpnPI CCDG 6 cut(s) 6, 43, 65, 117, 211, 238
MaeI CTAG 1 cut(s) 117
MboII GAAGA 2 cut(s) 38, 203
MhlI GDGCHC 1 cut(s) 46
MluCI AATT 2 cut(s) 108, 271
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 2 cut(s) 48, 244
MroXI GAANNNNTTC 1 cut(s) 195
MseI TTAA 1 cut(s) 269
MslI CAYNNNNRTG 1 cut(s) 128
MspI CCGG 2 cut(s) 30, 52
MspR9I CCNGG 1 cut(s) 226
MvaI CCWGG 1 cut(s) 226
MwoI GCNNNNNNNGC 2 cut(s) 26, 68
NlaIII CATG 3 cut(s) 133, 191, 203
NlaIV GGNNCC 1 cut(s) 102
NspI RCATGY 1 cut(s) 133
PagI TCATGA 2 cut(s) 187, 199
PciSI GCTCTTC 2 cut(s) 51, 186
PdmI GAANNNNTTC 1 cut(s) 195
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
Psp6I CCWGG 1 cut(s) 224
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 1 cut(s) 224
PspN4I GGNNCC 1 cut(s) 102
PspPI GGNCC 1 cut(s) 13
RseI CAYNNNNRTG 1 cut(s) 128
SapI GCTCTTC 2 cut(s) 51, 186
SaqAI TTAA 1 cut(s) 269
Sau96I GGNCC 1 cut(s) 13
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 1 cut(s) 226
SduI GDGCHC 1 cut(s) 46
SetI ASST 1 cut(s) 198
SmiMI CAYNNNNRTG 1 cut(s) 128
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 2 cut(s) 108, 271
SsiI CCGC 1 cut(s) 212
SspMI CTAG 1 cut(s) 117
StyD4I CCNGG 1 cut(s) 224
TaaI ACNGT 1 cut(s) 79
TasI AATT 2 cut(s) 108, 271
Tru1I TTAA 1 cut(s) 269
Tru9I TTAA 1 cut(s) 269
TspDTI ATGAA 4 cut(s) 176, 188, 204, 216
XceI RCATGY 1 cut(s) 133
XmnI GAANNNNTTC 1 cut(s) 195
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.