Rw0G019340

lysM domain receptor-like kinase 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00867
OG0023521
Physical Location & Seq
Reverse (-)
6591 .. 7322
732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G019340.1

Sequence Viewer

Length: 732 bp
ATGAGCCTCTTCAACAATCTCCACCCAATAAAACCACCCAGCCATGTACTGTTGGTGCCACCAGCCACCCATATCTACAAGTCCAAAATGGCCGTCGACACCGCCTTGCCCACCTCCTCCCCTCGACCCACAACCCAATTGTCCGAATCCGCCCGGACCCGGACCCGTACCCAAACCCGACCCACCTTCACCTCCTCCTCCTCCAAGCCCTCCACCTCCAGCTCAAACACAAGCTACAAGCTCTCCTCCACCGCCTCCACCTCCAGCCAAACCTCCCTTGCCAGCTTCCACCTCTCCCTCCCCGAAAACCCTAACCTCTACGACCTCTTCGAAATCTGCGCCGCCACCAACAACTTCCTCGCCAAGCGCTTCAGCTCCTCCTCTTTCACCCCCTCCTGGCGCTGCACCCTCCGTGGCAGAGACGCTGTCGTCTTCCAGAGCAAGTTCCGCCACAGGCTCGAAACCCAAGAACTCAAGAACCGGCTCTCCATGCTCTGCCGCAGCCACCACACCTCCATCGTCAAGCTCTTCGGCGCCTCCATCTCCGGCGACAACGTCTTCTTGGTCTACGAATTTGTAAGCAACTGCGCCAGCCTCGCTGCGTGTCTGAGACACAAGAACAACCCCAATTTCACCGTGCTATCGACGTGGGCGTCAAGAATGCAGGTCGCCGCCGACCTCGCCCAGGGCCTCAATTATATTCACAATAACACAAGCCTCAATGTCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

243

Amino Acids

26.91

Weight (kDa)

10.08

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 152 - 238 2.8e-08 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023521)

Species Orthologous Gene IDs
rosa_samantha Rh1BG268300 Rh1DG298400
rosa_wichuraiana Rw0G019340 Rw4G006810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 428, 652
Acc36I ACCTGC 1 cut(s) 655
AccB1I GGYRCC 2 cut(s) 55, 533
AccI GTMKAC 2 cut(s) 96, 567
AciI CCGC 7 cut(s) 102, 150, 252, 342, 448, 499, 672
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 1 cut(s) 572
AcuI CTGAAG 1 cut(s) 355
AcyI GRCGYC 2 cut(s) 534, 653
AfaI GTAC 2 cut(s) 48, 169
AfeI AGCGCT 1 cut(s) 368
AfiI CCNNNNNNNGG 3 cut(s) 159, 396, 685
AgsI TTSAA 1 cut(s) 13
AjiI CACGTC 1 cut(s) 648
AjnI CCWGG 2 cut(s) 395, 684
AloI GAACNNNNNNTCC 2 cut(s) 470, 502
AluBI AGCT 6 cut(s) 222, 234, 241, 285, 375, 526
AluI AGCT 6 cut(s) 222, 234, 241, 285, 375, 526
Alw26I GTCTC 2 cut(s) 414, 604
Aor51HI AGCGCT 1 cut(s) 368
AoxI GGCC 2 cut(s) 90, 688
ApeKI GCWGC 3 cut(s) 402, 501, 599
ApoI RAATTY 1 cut(s) 572
ArsI GACNNNNNNTTYG 2 cut(s) 78, 110
AspLEI GCGC 5 cut(s) 341, 369, 402, 536, 590
AspS9I GGNCC 3 cut(s) 156, 162, 688
AsuC2I CCSGG 2 cut(s) 154, 160
AsuHPI GGTGA 3 cut(s) 181, 379, 625
AsuII TTCGAA 1 cut(s) 330
AvaII GGWCC 2 cut(s) 156, 162
BanI GGYRCC 2 cut(s) 55, 533
BbsI GAAGAC 2 cut(s) 424, 550
BbvI GCAGC 3 cut(s) 389, 513, 586
BccI CCATC 2 cut(s) 524, 548
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 2 cut(s) 397, 686
BcnI CCSGG 2 cut(s) 154, 160
BcoDI GTCTC 2 cut(s) 414, 604
BfoI RGCGCY 3 cut(s) 370, 403, 537
BfuAI ACCTGC 1 cut(s) 655
BisI GCNGC 6 cut(s) 342, 403, 499, 502, 600, 672
BlsI GCNGC 6 cut(s) 343, 404, 500, 503, 601, 673
Bme1390I CCNGG 4 cut(s) 154, 160, 397, 686
Bme18I GGWCC 2 cut(s) 156, 162
BmgBI CACGTC 1 cut(s) 648
BmgT120I GGNCC 3 cut(s) 156, 162, 688
BmiI GGNNCC 4 cut(s) 57, 158, 164, 535
BmrFI CCNGG 4 cut(s) 154, 160, 397, 686
BpiI GAAGAC 2 cut(s) 424, 550
BpmI CTGGAG 2 cut(s) 202, 247
Bpu14I TTCGAA 1 cut(s) 330
BpuEI CTTGAG 1 cut(s) 458
BpuMI CCSGG 2 cut(s) 154, 160
BsaHI GRCGYC 2 cut(s) 534, 653
BsaJI CCNNGG 3 cut(s) 412, 684, 685
BsaXI ACNNNNNCTCC 6 cut(s) 227, 257, 470, 497, 500, 527
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 396, 685
Bse118I RCCGGY 1 cut(s) 480
BseBI CCWGG 2 cut(s) 397, 686
BseDI CCNNGG 3 cut(s) 412, 684, 685
BseLI CCNNNNNNNGG 3 cut(s) 159, 396, 685
BseMII CTCAG 1 cut(s) 599
BseRI GAGGAG 7 cut(s) 106, 184, 187, 190, 235, 367, 370
BseXI GCAGC 3 cut(s) 389, 513, 586
BseYI CCCAGC 1 cut(s) 38
BsgI GTGCAG 1 cut(s) 388
BshFI GGCC 2 cut(s) 92, 690
BshNI GGYRCC 2 cut(s) 55, 533
BsiSI CCGG 4 cut(s) 154, 160, 481, 546
BslI CCNNNNNNNGG 3 cut(s) 159, 396, 685
BsmAI GTCTC 2 cut(s) 414, 604
BsmBI CGTCTC 1 cut(s) 414
BsmI GAATGC 1 cut(s) 666
BsnI GGCC 2 cut(s) 92, 690
Bsp119I TTCGAA 1 cut(s) 330
BspACI CCGC 7 cut(s) 102, 150, 252, 342, 448, 499, 672
BspANI GGCC 2 cut(s) 92, 690
BspCNI CTCAG 1 cut(s) 600
BspLI GGNNCC 4 cut(s) 57, 158, 164, 535
BspMI ACCTGC 1 cut(s) 655
BspQI GCTCTTC 1 cut(s) 533
BspT104I TTCGAA 1 cut(s) 330
BspT107I GGYRCC 2 cut(s) 55, 533
BsrFI RCCGGY 1 cut(s) 480
BssAI RCCGGY 1 cut(s) 480
BssECI CCNNGG 3 cut(s) 412, 684, 685
BssNI GRCGYC 2 cut(s) 534, 653
Bst2UI CCWGG 2 cut(s) 397, 686
Bst4CI ACNGT 2 cut(s) 51, 637
Bst6I CTCTTC 3 cut(s) 14, 332, 533
BstACI GRCGYC 2 cut(s) 534, 653
BstBI TTCGAA 1 cut(s) 330
BstC8I GCNNGC 2 cut(s) 283, 592
BstDEI CTNAG 1 cut(s) 608
BstDSI CCRYGG 1 cut(s) 412
BstENI CCTNNNNNAGG 1 cut(s) 683
BstH2I RGCGCY 3 cut(s) 370, 403, 537
BstHHI GCGC 5 cut(s) 341, 369, 402, 536, 590
BstMAI GTCTC 2 cut(s) 414, 604
BstMWI GCNNNNNNNGC 4 cut(s) 447, 490, 596, 680
BstNI CCWGG 2 cut(s) 397, 686
BstSCI CCNGG 4 cut(s) 152, 158, 395, 684
BstV1I GCAGC 3 cut(s) 389, 513, 586
BstV2I GAAGAC 2 cut(s) 424, 550
BsuRI GGCC 2 cut(s) 92, 690
BtgI CCRYGG 1 cut(s) 412
BtrI CACGTC 1 cut(s) 648
BveI ACCTGC 1 cut(s) 655
Cac8I GCNNGC 2 cut(s) 283, 592
CfoI GCGC 5 cut(s) 341, 369, 402, 536, 590
Cfr10I RCCGGY 1 cut(s) 480
Cfr13I GGNCC 3 cut(s) 156, 162, 688
CseI GACGC 2 cut(s) 431, 642
Csp6I GTAC 2 cut(s) 47, 168
CviAII CATG 2 cut(s) 44, 490
CviQI GTAC 2 cut(s) 47, 168
DdeI CTNAG 1 cut(s) 608
DinI GGCGCC 1 cut(s) 535
DrdI GACNNNNNNGTC 2 cut(s) 428, 652
DseDI GACNNNNNNGTC 2 cut(s) 428, 652
EaeI YGGCCR 1 cut(s) 90
Eam1104I CTCTTC 3 cut(s) 14, 332, 533
EarI CTCTTC 3 cut(s) 14, 332, 533
EciI GGCGGA 2 cut(s) 139, 437
Eco47I GGWCC 2 cut(s) 156, 162
Eco47III AGCGCT 1 cut(s) 368
Eco57I CTGAAG 1 cut(s) 355
EcoNI CCTNNNNNAGG 1 cut(s) 683
EcoO109I RGGNCCY 1 cut(s) 688
EcoRII CCWGG 2 cut(s) 395, 684
EgeI GGCGCC 1 cut(s) 535
EheI GGCGCC 1 cut(s) 535
Esp3I CGTCTC 1 cut(s) 414
FaeI CATG 2 cut(s) 47, 493
FaiI YATR 4 cut(s) 45, 72, 491, 699
FatI CATG 2 cut(s) 43, 489
FblI GTMKAC 2 cut(s) 96, 567
Fnu4HI GCNGC 6 cut(s) 342, 403, 499, 502, 600, 672
Fsp4HI GCNGC 6 cut(s) 342, 403, 499, 502, 600, 672
GlaI GCGC 5 cut(s) 340, 368, 401, 535, 589
GluI GCNGC 6 cut(s) 342, 403, 499, 502, 600, 672
GsaI CCCAGC 1 cut(s) 42
GsuI CTGGAG 2 cut(s) 202, 247
HaeII RGCGCY 3 cut(s) 370, 403, 537
HaeIII GGCC 2 cut(s) 92, 690
HapII CCGG 4 cut(s) 154, 160, 481, 546
HgaI GACGC 2 cut(s) 431, 642
HhaI GCGC 5 cut(s) 341, 369, 402, 536, 590
Hin1I GRCGYC 2 cut(s) 534, 653
Hin1II CATG 2 cut(s) 47, 493
Hin6I GCGC 5 cut(s) 339, 367, 400, 534, 588
HinP1I GCGC 5 cut(s) 339, 367, 400, 534, 588
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 1 cut(s) 146
HpaII CCGG 4 cut(s) 154, 160, 481, 546
HphI GGTGA 3 cut(s) 181, 379, 625
Hpy166II GTNNAC 2 cut(s) 97, 568
Hpy188I TCNGA 2 cut(s) 145, 609
Hpy188III TCNNGA 3 cut(s) 436, 475, 657
Hpy8I GTNNAC 2 cut(s) 97, 568
Hpy99I CGWCG 2 cut(s) 98, 649
HpyAV CCTTC 1 cut(s) 196
HpyCH4III ACNGT 2 cut(s) 51, 637
HpyCH4IV ACGT 2 cut(s) 555, 647
HpyCH4V TGCA 2 cut(s) 405, 664
HpyF10VI GCNNNNNNNGC 4 cut(s) 447, 490, 596, 680
HpyF3I CTNAG 1 cut(s) 608
HpySE526I ACGT 2 cut(s) 555, 647
Hsp92I GRCGYC 2 cut(s) 534, 653
Hsp92II CATG 2 cut(s) 47, 493
HspAI GCGC 5 cut(s) 339, 367, 400, 534, 588
KasI GGCGCC 1 cut(s) 533
LguI GCTCTTC 1 cut(s) 533
LmnI GCTCC 1 cut(s) 380
Lsp1109I GCAGC 3 cut(s) 389, 513, 586
MaeII ACGT 2 cut(s) 555, 647
MboII GAAGA 4 cut(s) 319, 424, 520, 550
MfeI CAATTG 1 cut(s) 137
MluCI AATT 4 cut(s) 137, 572, 628, 694
Mly113I GGCGCC 1 cut(s) 534
MspI CCGG 4 cut(s) 154, 160, 481, 546
MspR9I CCNGG 4 cut(s) 154, 160, 397, 686
MunI CAATTG 1 cut(s) 137
Mva1269I GAATGC 1 cut(s) 666
MvaI CCWGG 2 cut(s) 397, 686
MwoI GCNNNNNNNGC 4 cut(s) 447, 490, 596, 680
NarI GGCGCC 1 cut(s) 534
NciI CCSGG 2 cut(s) 154, 160
NlaIII CATG 2 cut(s) 47, 493
NlaIV GGNNCC 4 cut(s) 57, 158, 164, 535
NspV TTCGAA 1 cut(s) 330
PasI CCCWGGG 1 cut(s) 685
PciSI GCTCTTC 1 cut(s) 533
PcsI WCGNNNNNNNCGW 2 cut(s) 327, 650
PctI GAATGC 1 cut(s) 666
PfeI GAWTC 1 cut(s) 146
PflFI GACNNNGTC 2 cut(s) 425, 554
PkrI GCNGC 6 cut(s) 343, 404, 500, 503, 601, 673
PluTI GGCGCC 1 cut(s) 537
Psp6I CCWGG 2 cut(s) 395, 684
PspFI CCCAGC 1 cut(s) 38
PspGI CCWGG 2 cut(s) 395, 684
PspN4I GGNNCC 4 cut(s) 57, 158, 164, 535
PspPI GGNCC 3 cut(s) 156, 162, 688
PsyI GACNNNGTC 2 cut(s) 425, 554
RsaI GTAC 2 cut(s) 48, 169
RsaNI GTAC 2 cut(s) 47, 168
SalI GTCGAC 1 cut(s) 95
SapI GCTCTTC 1 cut(s) 533
SatI GCNGC 6 cut(s) 342, 403, 499, 502, 600, 672
Sau96I GGNCC 3 cut(s) 156, 162, 688
ScrFI CCNGG 4 cut(s) 154, 160, 397, 686
SfoI GGCGCC 1 cut(s) 535
SfuI TTCGAA 1 cut(s) 330
SinI GGWCC 2 cut(s) 156, 162
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
Sse9I AATT 4 cut(s) 137, 572, 628, 694
SsiI CCGC 7 cut(s) 102, 150, 252, 342, 448, 499, 672
SspDI GGCGCC 1 cut(s) 533
StyD4I CCNGG 4 cut(s) 152, 158, 395, 684
TaaI ACNGT 2 cut(s) 51, 637
TaiI ACGT 2 cut(s) 558, 650
TaqI TCGA 5 cut(s) 96, 124, 330, 459, 644
TasI AATT 4 cut(s) 137, 572, 628, 694
TatI WGTACW 1 cut(s) 46
TauI GCSGC 3 cut(s) 344, 501, 674
TfiI GAWTC 1 cut(s) 146
TseI GCWGC 3 cut(s) 402, 501, 599
TspGWI ACGGA 1 cut(s) 401
Tth111I GACNNNGTC 2 cut(s) 425, 554
VpaK11BI GGWCC 2 cut(s) 156, 162
XagI CCTNNNNNAGG 1 cut(s) 683
XapI RAATTY 1 cut(s) 572
XmiI GTMKAC 2 cut(s) 96, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.