Rw2G028240

F-box associated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0021614
Physical Location & Seq
Reverse (-)
45038517 .. 45040453
1937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G028240.1

Sequence Viewer

Length: 1176 bp
ATGAGAAGGAAAAGAGCGAGGAAGCCAAAAGCTGACATCCAAATAATTAAAAACAATGAACAAATTCAACAAATTGTGGATGGTCCTTTTACAACAGATCTTCCGAAGTCTATAGTTCAAGTAATTTTAGTAAATCTCCCAGTAAAGAGCATTCTCATTTGCAAAATTGTGTGCAAGACTTGGCATGGTGTAATTTCGGACCCTGAATTTACTCATATGCACTTGCATTTGAACCAAGAAGAGGCCTATCCTACGTTCCGACCCTTGCATAGATGGCTATTACCAATTGCAAAATTCGGTTGCGCAATCCTGGGGAGGCATTCAACTACCACAATAAGCTCATTAATTATATGCTTAACAGGAAACGTGGTGCTAAAAAGGAAACATAGATCTTGCATCAACGACATAAATCCACTCGATCACAGGTACATGTTGGTGAATTCGTGTAACGGCTTGCTTTGCTTGGCTGAAGAATGCATTGGACCTTCTGTCGTCTGCAATCCAATAACTGGCGACCACAAGAGATGTATTAGTCGAGGATTTGGTTTCAGTCCGAAGAATAACAAGTATAAGGTGATAAGAGTCATTCACAAAGGAGCAGAGCATCCCATTCGGGTGGTTGATATAGAGATACACACACTTGGGACTCATTCATGGAAAGATGTTGGTAGTTCTCCCTTCGCCGAGCATATGGCATATCCCAGCAGTTTGGATTGCATAGCTTCTTTTGACTTTGACAATGAGTTGTTTCAGTCAGTTCCACCACTGCTTCTTCAGCTGGATACTTGTGACTGGGGGATGGGGGTTTTGGGAGATATTCTTTCTGTGTGTGGTACTCACGATAATCATATCGATGTGTGGACAGTGGAAGATCATGATGCACAAAAGTCCTGGACCAAAAAGGTTTATATTAATATGGATGTTGGGGGAGGACTACTAGGGTGGCCAAAGGGCACATATATACCGATGAAATACTTAACAGATGGGGCAGATGGGGCTCTATTGATGTTTAATTGGGATATTGATGCCTTGGTTTACCATCACCCAAGAAGAAACTACAGATCGATCATTATCTTGACCTTAAGATTAGAGGTGTATACGGAGAAGATCGAGCATGATGTAGAAGTACTGAATGTGTATTTAAGAGGTCTTAAATTGAAGACGAATGAGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

391

Amino Acids

44.47

Weight (kDa)

8.17

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 33 - 71 2.4e-06 F-box domain
F-box-like PF12937 33 - 69 4e-06 F-box-like
FBA_1 PF07734 126 - 309 3.7e-09 F-box associated beta propeller domain
FBA_3 PF08268 141 - 304 3e-06 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021614)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8057844.1_g000001 Rmu_ssc0000019.1_g000001
rosa_samantha Rh2BG355100 Rh2DG372800
rosa_wichuraiana Rw2G028240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 304
AccB7I CCANNNNNTGG 1 cut(s) 509
AccI GTMKAC 1 cut(s) 1097
AcoI YGGCCR 1 cut(s) 944
AcsI RAATTY 4 cut(s) 63, 206, 293, 439
AcuI CTGAAG 2 cut(s) 489, 758
AfaI GTAC 3 cut(s) 428, 835, 1128
AfiI CCNNNNNNNGG 2 cut(s) 241, 509
AflII CTTAAG 1 cut(s) 1081
AflIII ACRYGT 1 cut(s) 429
AgsI TTSAA 5 cut(s) 68, 119, 232, 324, 1159
AhdI GACNNNNNGTC 1 cut(s) 488
AjnI CCWGG 2 cut(s) 309, 890
AjuI GAANNNNNNNTTGG 2 cut(s) 462, 494
AluBI AGCT 4 cut(s) 32, 339, 722, 778
AluI AGCT 4 cut(s) 32, 339, 722, 778
AoxI GGCC 2 cut(s) 243, 944
ApoI RAATTY 4 cut(s) 63, 206, 293, 439
AseI ATTAAT 2 cut(s) 344, 912
Asp700I GAANNNNTTC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 4 cut(s) 83, 199, 482, 894
AsuHPI GGTGA 3 cut(s) 448, 586, 1034
AvaII GGWCC 4 cut(s) 83, 199, 482, 894
BaeGI GKGCMC 1 cut(s) 956
BalI TGGCCA 1 cut(s) 946
BanII GRGCYC 1 cut(s) 1000
BbsI GAAGAC 1 cut(s) 1166
BccI CCATC 6 cut(s) 74, 267, 793, 977, 986, 1047
BceAI ACGGC 1 cut(s) 466
BcgI CGANNNNNNTGC 2 cut(s) 593, 627
BciT130I CCWGG 2 cut(s) 311, 892
BciVI GTATCC 1 cut(s) 775
BfaI CTAG 1 cut(s) 938
BfmI CTRYAG 2 cut(s) 111, 1057
BfrI CTTAAG 1 cut(s) 1081
BfuI GTATCC 1 cut(s) 775
BglII AGATCT 2 cut(s) 97, 389
BmcAI AGTACT 1 cut(s) 1128
Bme1390I CCNGG 2 cut(s) 311, 892
Bme18I GGWCC 4 cut(s) 83, 199, 482, 894
BmeRI GACNNNNNGTC 1 cut(s) 488
BmgT120I GGNCC 4 cut(s) 83, 199, 482, 894
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 2 cut(s) 311, 892
BmrI ACTGGG 2 cut(s) 134, 802
BmsI GCATC 4 cut(s) 405, 613, 868, 1015
BmuI ACTGGG 2 cut(s) 134, 802
BpiI GAAGAC 1 cut(s) 1166
Bsa29I ATCGAT 2 cut(s) 852, 1064
BsaBI GATNNNNATC 1 cut(s) 1070
BsaJI CCNNGG 2 cut(s) 310, 1029
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 509
Bse1I ACTGG 3 cut(s) 140, 514, 797
Bse8I GATNNNNATC 1 cut(s) 1070
BseBI CCWGG 2 cut(s) 311, 892
BseCI ATCGAT 2 cut(s) 852, 1064
BseDI CCNNGG 2 cut(s) 310, 1029
BseGI GGATG 5 cut(s) 36, 85, 604, 804, 925
BseJI GATNNNNATC 1 cut(s) 1070
BseLI CCNNNNNNNGG 2 cut(s) 241, 509
BseNI ACTGG 3 cut(s) 140, 514, 797
BseSI GKGCMC 1 cut(s) 956
BseYI CCCAGC 1 cut(s) 701
BshFI GGCC 2 cut(s) 245, 946
BshVI ATCGAT 2 cut(s) 852, 1064
BslFI GGGAC 1 cut(s) 658
BslI CCNNNNNNNGG 2 cut(s) 241, 509
BsmFI GGGAC 1 cut(s) 658
BsmI GAATGC 3 cut(s) 150, 319, 479
BsnI GGCC 2 cut(s) 245, 946
Bsp1286I GDGCHC 2 cut(s) 956, 1000
Bsp143I GATC 7 cut(s) 97, 389, 418, 871, 1061, 1065, 1107
BspANI GGCC 2 cut(s) 245, 946
BspDI ATCGAT 2 cut(s) 852, 1064
BspHI TCATGA 1 cut(s) 874
BspLI GGNNCC 1 cut(s) 201
BspTI CTTAAG 1 cut(s) 1081
BsrI ACTGG 3 cut(s) 140, 514, 797
BssECI CCNNGG 2 cut(s) 310, 1029
BssMI GATC 7 cut(s) 97, 389, 418, 871, 1061, 1065, 1107
BssNAI GTATAC 1 cut(s) 1098
BssT1I CCWWGG 1 cut(s) 1029
Bst1107I GTATAC 1 cut(s) 1098
Bst2UI CCWGG 2 cut(s) 311, 892
Bst4CI ACNGT 1 cut(s) 865
Bst6I CTCTTC 1 cut(s) 234
BstAFI CTTAAG 1 cut(s) 1081
BstC8I GCNNGC 1 cut(s) 455
BstF5I GGATG 5 cut(s) 36, 85, 604, 804, 925
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 7 cut(s) 100, 392, 421, 874, 1064, 1068, 1110
BstMBI GATC 7 cut(s) 97, 389, 418, 871, 1061, 1065, 1107
BstMWI GCNNNNNNNGC 4 cut(s) 274, 459, 775, 995
BstNI CCWGG 2 cut(s) 311, 892
BstNSI RCATGY 1 cut(s) 433
BstSCI CCNGG 2 cut(s) 309, 890
BstSFI CTRYAG 2 cut(s) 111, 1057
BstSLI GKGCMC 1 cut(s) 956
BstV2I GAAGAC 1 cut(s) 1166
BstX2I RGATCY 2 cut(s) 97, 389
BstXI CCANNNNNNTGG 2 cut(s) 616, 709
BstYI RGATCY 2 cut(s) 97, 389
BstZ17I GTATAC 1 cut(s) 1098
Bsu15I ATCGAT 2 cut(s) 852, 1064
BsuI GTATCC 1 cut(s) 775
BsuRI GGCC 2 cut(s) 245, 946
BsuTUI ATCGAT 2 cut(s) 852, 1064
BtsCI GGATG 5 cut(s) 36, 85, 604, 804, 925
BtsI GCAGTG 1 cut(s) 764
BtsIMutI CAGTG 2 cut(s) 764, 870
Cac8I GCNNGC 1 cut(s) 455
CciI TCATGA 1 cut(s) 874
CfoI GCGC 1 cut(s) 305
Cfr13I GGNCC 4 cut(s) 83, 199, 482, 894
ClaI ATCGAT 2 cut(s) 852, 1064
Csp6I GTAC 3 cut(s) 427, 834, 1127
CviAII CATG 5 cut(s) 185, 430, 654, 875, 1115
CviQI GTAC 3 cut(s) 427, 834, 1127
DpnI GATC 7 cut(s) 99, 391, 420, 873, 1063, 1067, 1109
DpnII GATC 7 cut(s) 97, 389, 418, 871, 1061, 1065, 1107
DriI GACNNNNNGTC 1 cut(s) 488
EaeI YGGCCR 1 cut(s) 944
Eam1104I CTCTTC 1 cut(s) 234
Eam1105I GACNNNNNGTC 1 cut(s) 488
EarI CTCTTC 1 cut(s) 234
Eco130I CCWWGG 1 cut(s) 1029
Eco147I AGGCCT 1 cut(s) 245
Eco24I GRGCYC 1 cut(s) 1000
Eco47I GGWCC 4 cut(s) 83, 199, 482, 894
Eco57I CTGAAG 2 cut(s) 489, 758
EcoRI GAATTC 1 cut(s) 439
EcoRII CCWGG 2 cut(s) 309, 890
EcoT14I CCWWGG 1 cut(s) 1029
EcoT22I ATGCAT 1 cut(s) 479
EcoT38I GRGCYC 1 cut(s) 1000
ErhI CCWWGG 1 cut(s) 1029
FaeI CATG 5 cut(s) 188, 433, 657, 878, 1118
FaqI GGGAC 1 cut(s) 658
FatI CATG 5 cut(s) 184, 429, 653, 874, 1114
FauNDI CATATG 2 cut(s) 216, 690
FblI GTMKAC 1 cut(s) 1097
FokI GGATG 5 cut(s) 23, 92, 591, 811, 932
FriOI GRGCYC 1 cut(s) 1000
FspBI CTAG 1 cut(s) 938
FspI TGCGCA 1 cut(s) 304
GlaI GCGC 1 cut(s) 304
GsaI CCCAGC 1 cut(s) 705
HaeIII GGCC 2 cut(s) 245, 946
HhaI GCGC 1 cut(s) 305
Hin1II CATG 5 cut(s) 188, 433, 657, 878, 1118
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HinfI GANTC 2 cut(s) 582, 646
HphI GGTGA 3 cut(s) 448, 586, 1034
Hpy166II GTNNAC 3 cut(s) 861, 1036, 1098
Hpy188I TCNGA 4 cut(s) 105, 199, 260, 555
Hpy188III TCNNGA 3 cut(s) 839, 875, 1075
Hpy8I GTNNAC 3 cut(s) 861, 1036, 1098
HpyAV CCTTC 2 cut(s) 495, 688
HpyCH4III ACNGT 1 cut(s) 865
HpyCH4IV ACGT 2 cut(s) 254, 366
HpyF10VI GCNNNNNNNGC 4 cut(s) 274, 459, 775, 995
HpySE526I ACGT 2 cut(s) 254, 366
Hsp92II CATG 5 cut(s) 188, 433, 657, 878, 1118
HspAI GCGC 1 cut(s) 303
Kzo9I GATC 7 cut(s) 97, 389, 418, 871, 1061, 1065, 1107
LmnI GCTCC 1 cut(s) 596
LweI GCATC 4 cut(s) 405, 613, 868, 1015
MaeI CTAG 1 cut(s) 938
MaeII ACGT 2 cut(s) 254, 366
MaeIII GTNAC 2 cut(s) 446, 788
MalI GATC 7 cut(s) 99, 391, 420, 873, 1063, 1067, 1109
MboI GATC 7 cut(s) 97, 389, 418, 871, 1061, 1065, 1107
MboII GAAGA 9 cut(s) 92, 251, 482, 568, 764, 881, 1062, 1117, 1171
MfeI CAATTG 1 cut(s) 285
MflI RGATCY 2 cut(s) 97, 389
MhlI GDGCHC 2 cut(s) 956, 1000
MlsI TGGCCA 1 cut(s) 946
MluNI TGGCCA 1 cut(s) 946
MlyI GAGTC 2 cut(s) 591, 640
MmeI TCCRAC 1 cut(s) 283
MnlI CCTC 7 cut(s) 12, 235, 309, 530, 923, 1084, 1139
Mox20I TGGCCA 1 cut(s) 946
Mph1103I ATGCAT 1 cut(s) 479
MroXI GAANNNNTTC 1 cut(s) 63
MscI TGGCCA 1 cut(s) 946
MseI TTAA 9 cut(s) 48, 344, 356, 912, 977, 1011, 1082, 1142, 1152
MslI CAYNNNNRTG 3 cut(s) 434, 614, 852
Msp20I TGGCCA 1 cut(s) 946
MspA1I CMGCKG 1 cut(s) 778
MspCI CTTAAG 1 cut(s) 1081
MspR9I CCNGG 2 cut(s) 311, 892
MunI CAATTG 1 cut(s) 285
Mva1269I GAATGC 3 cut(s) 150, 319, 479
MvaI CCWGG 2 cut(s) 311, 892
MwoI GCNNNNNNNGC 4 cut(s) 274, 459, 775, 995
NdeI CATATG 2 cut(s) 216, 690
NdeII GATC 7 cut(s) 97, 389, 418, 871, 1061, 1065, 1107
NlaIII CATG 5 cut(s) 188, 433, 657, 878, 1118
NlaIV GGNNCC 1 cut(s) 201
NmeAIII GCCGAG 1 cut(s) 709
NmuCI GTSAC 1 cut(s) 788
NsbI TGCGCA 1 cut(s) 304
NsiI ATGCAT 1 cut(s) 479
NspI RCATGY 1 cut(s) 433
PagI TCATGA 1 cut(s) 874
PceI AGGCCT 1 cut(s) 245
PciI ACATGT 1 cut(s) 429
PctI GAATGC 3 cut(s) 150, 319, 479
PdmI GAANNNNTTC 1 cut(s) 63
PflMI CCANNNNNTGG 1 cut(s) 509
PfoI TCCNGGA 1 cut(s) 890
PleI GAGTC 2 cut(s) 590, 640
PpsI GAGTC 2 cut(s) 590, 640
PscI ACATGT 1 cut(s) 429
PshBI ATTAAT 2 cut(s) 344, 912
Psp6I CCWGG 2 cut(s) 309, 890
PspFI CCCAGC 1 cut(s) 701
PspGI CCWGG 2 cut(s) 309, 890
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 4 cut(s) 83, 199, 482, 894
PsuI RGATCY 2 cut(s) 97, 389
PvuII CAGCTG 1 cut(s) 778
RsaI GTAC 3 cut(s) 428, 835, 1128
RsaNI GTAC 3 cut(s) 427, 834, 1127
RseI CAYNNNNRTG 3 cut(s) 434, 614, 852
SaqAI TTAA 9 cut(s) 48, 344, 356, 912, 977, 1011, 1082, 1142, 1152
Sau3AI GATC 7 cut(s) 97, 389, 418, 871, 1061, 1065, 1107
Sau96I GGNCC 4 cut(s) 83, 199, 482, 894
ScaI AGTACT 1 cut(s) 1128
SchI GAGTC 2 cut(s) 591, 640
ScrFI CCNGG 2 cut(s) 311, 892
SduI GDGCHC 2 cut(s) 956, 1000
SfaNI GCATC 4 cut(s) 405, 613, 868, 1015
SfcI CTRYAG 2 cut(s) 111, 1057
SinI GGWCC 4 cut(s) 83, 199, 482, 894
SmiMI CAYNNNNRTG 3 cut(s) 434, 614, 852
SmlI CTYRAG 1 cut(s) 1081
SmoI CTYRAG 1 cut(s) 1081
SseBI AGGCCT 1 cut(s) 245
SspMI CTAG 1 cut(s) 938
StuI AGGCCT 1 cut(s) 245
StyD4I CCNGG 2 cut(s) 309, 890
StyI CCWWGG 1 cut(s) 1029
TaaI ACNGT 1 cut(s) 865
TaiI ACGT 2 cut(s) 257, 369
TaqI TCGA 5 cut(s) 417, 535, 852, 1064, 1110
TatI WGTACW 1 cut(s) 1126
Tru1I TTAA 9 cut(s) 48, 344, 356, 912, 977, 1011, 1082, 1142, 1152
Tru9I TTAA 9 cut(s) 48, 344, 356, 912, 977, 1011, 1082, 1142, 1152
TscAI CASTG 2 cut(s) 771, 870
TseFI GTSAC 1 cut(s) 788
Tsp45I GTSAC 1 cut(s) 788
TspDTI ATGAA 3 cut(s) 72, 642, 983
TspGWI ACGGA 1 cut(s) 1115
TspRI CASTG 2 cut(s) 771, 870
Van91I CCANNNNNTGG 1 cut(s) 509
Vha464I CTTAAG 1 cut(s) 1081
VpaK11BI GGWCC 4 cut(s) 83, 199, 482, 894
VspI ATTAAT 2 cut(s) 344, 912
XapI RAATTY 4 cut(s) 63, 206, 293, 439
XceI RCATGY 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 1097
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 1 cut(s) 938
ZrmI AGTACT 1 cut(s) 1128
Zsp2I ATGCAT 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.