Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0017561

Physical Location & Seq

Genomic Coordinates Reverse (-)

39943657 .. 39948223

Gene Structure 4567 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G027710.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 684 bp

ATGAAGTCTTGTATAGCACTTACTGGCCTTGTGGTCTTCCTCTCTGCGATTTTTGAGTTGGGGCTTTGCAGGGAGGACCCTCTCATCAAAATGAATCTGGGTGGTTTTAAAGATTCTCGCGCTTCTCAGAACAGCGCGGAAAGTATCGCTCTCTTTGCTGTCCAAGAACACAACAAGCAACAGAATGGTGTGCTTGAGTTTGCGAGGGTGTTGAATTCAAGGGAACAAGTGGTTGCTGGTAAGATTTACCATCTCACGCTGGAAGCTGTTGATGCTGGTATCAACAAGATATATGAAGCTAAGGTCTGGGTGAAACCATGGATCAACTTCAAGCAGTTGCAGGAGTTTAAGCTCGCACACCATGTCCCTTCCTTCACTTCTTCGGACCTCGGCCTTAAACCAGATGGCCATGGACCAGGATGGCGACCAGTGCCAATTCATGATCCTGAGGTCCAAGATGCAGCAACTCATGCTGTTCAGTCCCTTCAGCTCCGATCAAATTCGTTGTACCCATATGAACTTCTTGAGATCCTCCTAGCTAAGGCTAAGGTTATTGAAGATTATGCCAGATTTCATTTGCTTTTGAAGGTGAAGAGGGGAATACAAGAACAGAAGCTCTGGGTTGAAGTAAATAAGAACATTGAAGGAAGGTTTTATTCGAGTGGGATGGAAAGAGATCCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002020 GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0005488 GO:0005515 GO:0008150 GO:0009892 GO:0010466 GO:0010605 GO:0010951 GO:0019222 GO:0019899 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0043086 GO:0044092 GO:0045861 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cystatin SQAPI

Protein Analysis

227

Amino Acids

25.63

Weight (kDa)

7.8

Isoelectric Point (pI)

46.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cystatin	PF00031	34 - 119	5.5e-13	Cystatin domain
SQAPI	PF16845	44 - 117	2.7e-20	Aspartic acid proteinase inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017561)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05110
rosa_chinensis	RchiOBHm_Chr3g0494971
rosa_laevigata	RLG00000022659
rosa_multiflora	Rmu_sc0003482.1_g000015
rosa_roxburghii	Rroxscaffold_6G00390870
rosa_rugosa	Rorug03G0268500 Rorug03G0268600
rosa_samantha	Rh3AG318100 Rh3BG352900 Rh3CG349800 Rh3DG352200
rosa_wichuraiana	Rw3G027710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	120, 137
AciI	CCGC	1 cut(s)	137
AclWI	GGATC	4 cut(s)	329, 437, 523, 671
AcoI	YGGCCR	1 cut(s)	406
AcsI	RAATTY	2 cut(s)	214, 499
AcuI	CTGAAG	1 cut(s)	470
AfaI	GTAC	1 cut(s)	509
AfiI	CCNNNNNNNGG	1 cut(s)	541
AgsI	TTSAA	7 cut(s)	214, 219, 331, 557, 586, 626, 644
AjnI	CCWGG	1 cut(s)	415
AluBI	AGCT	6 cut(s)	266, 299, 352, 490, 539, 616
AluI	AGCT	6 cut(s)	266, 299, 352, 490, 539, 616
AlwI	GGATC	4 cut(s)	329, 437, 523, 671
AoxI	GGCC	3 cut(s)	25, 391, 406
ApeKI	GCWGC	1 cut(s)	461
ApoI	RAATTY	2 cut(s)	214, 499
AspLEI	GCGC	2 cut(s)	122, 137
AspS9I	GGNCC	4 cut(s)	76, 385, 413, 451
AsuHPI	GGTGA	2 cut(s)	322, 601
AvaII	GGWCC	4 cut(s)	76, 385, 413, 451
AxyI	CCTNAGG	1 cut(s)	447
BalI	TGGCCA	1 cut(s)	408
BbsI	GAAGAC	1 cut(s)	28
BbvI	GCAGC	1 cut(s)	473
BccI	CCATC	4 cut(s)	258, 398, 414, 661
BciT130I	CCWGG	1 cut(s)	417
BfaI	CTAG	1 cut(s)	536
BisI	GCNGC	1 cut(s)	462
BlsI	GCNGC	1 cut(s)	463
Bme1390I	CCNGG	1 cut(s)	417
Bme18I	GGWCC	4 cut(s)	76, 385, 413, 451
BmgT120I	GGNCC	4 cut(s)	76, 385, 413, 451
BmiI	GGNNCC	1 cut(s)	78
BmrFI	CCNGG	1 cut(s)	417
BmsI	GCATC	2 cut(s)	262, 448
BpiI	GAAGAC	1 cut(s)	28
Bpu10I	CCTNAGC	3 cut(s)	300, 540, 546
BpuEI	CTTGAG	2 cut(s)	215, 545
BsaJI	CCNNGG	3 cut(s)	317, 388, 409
Bsc4I	CCNNNNNNNGG	1 cut(s)	541
Bse1I	ACTGG	2 cut(s)	28, 428
Bse21I	CCTNAGG	1 cut(s)	447
BseBI	CCWGG	1 cut(s)	417
BseDI	CCNNGG	3 cut(s)	317, 388, 409
BseGI	GGATG	2 cut(s)	425, 672
BseLI	CCNNNNNNNGG	1 cut(s)	541
BseMII	CTCAG	2 cut(s)	140, 438
BseNI	ACTGG	2 cut(s)	28, 428
BseXI	GCAGC	1 cut(s)	473
Bsh1236I	CGCG	2 cut(s)	120, 137
BshFI	GGCC	3 cut(s)	27, 393, 408
BslFI	GGGAC	2 cut(s)	350, 466
BslI	CCNNNNNNNGG	1 cut(s)	541
BsmFI	GGGAC	2 cut(s)	350, 466
BsnI	GGCC	3 cut(s)	27, 393, 408
Bsp143I	GATC	5 cut(s)	321, 442, 494, 528, 676
Bsp19I	CCATGG	2 cut(s)	317, 409
BspACI	CCGC	1 cut(s)	137
BspANI	GGCC	3 cut(s)	27, 393, 408
BspCNI	CTCAG	2 cut(s)	139, 439
BspFNI	CGCG	2 cut(s)	120, 137
BspHI	TCATGA	1 cut(s)	439
BspLI	GGNNCC	1 cut(s)	78
BspPI	GGATC	4 cut(s)	329, 437, 523, 671
BsrI	ACTGG	2 cut(s)	28, 428
BssECI	CCNNGG	3 cut(s)	317, 388, 409
BssMI	GATC	5 cut(s)	321, 442, 494, 528, 676
BssT1I	CCWWGG	2 cut(s)	317, 409
Bst2UI	CCWGG	1 cut(s)	417
Bst6I	CTCTTC	1 cut(s)	587
BstAPI	GCANNNNNTGC	1 cut(s)	470
BstC8I	GCNNGC	1 cut(s)	354
BstDEI	CTNAG	5 cut(s)	126, 300, 447, 540, 546
BstDSI	CCRYGG	2 cut(s)	317, 409
BstENI	CCTNNNNNAGG	1 cut(s)	539
BstF5I	GGATG	2 cut(s)	425, 672
BstFNI	CGCG	2 cut(s)	120, 137
BstHHI	GCGC	2 cut(s)	122, 137
BstKTI	GATC	5 cut(s)	324, 445, 497, 531, 679
BstMBI	GATC	5 cut(s)	321, 442, 494, 528, 676
BstMWI	GCNNNNNNNGC	4 cut(s)	155, 272, 430, 470
BstNI	CCWGG	1 cut(s)	417
BstSCI	CCNGG	1 cut(s)	415
BstUI	CGCG	2 cut(s)	120, 137
BstV1I	GCAGC	1 cut(s)	473
BstV2I	GAAGAC	1 cut(s)	28
BstX2I	RGATCY	2 cut(s)	528, 676
BstYI	RGATCY	2 cut(s)	528, 676
Bsu36I	CCTNAGG	1 cut(s)	447
BsuRI	GGCC	3 cut(s)	27, 393, 408
BtgI	CCRYGG	2 cut(s)	317, 409
BtsCI	GGATG	2 cut(s)	425, 672
BtsIMutI	CAGTG	1 cut(s)	435
Cac8I	GCNNGC	1 cut(s)	354
CciI	TCATGA	1 cut(s)	439
CfoI	GCGC	2 cut(s)	122, 137
Cfr13I	GGNCC	4 cut(s)	76, 385, 413, 451
Csp6I	GTAC	1 cut(s)	508
CviAII	CATG	5 cut(s)	318, 362, 410, 440, 470
CviQI	GTAC	1 cut(s)	508
DdeI	CTNAG	5 cut(s)	126, 300, 447, 540, 546
DpnI	GATC	5 cut(s)	323, 444, 496, 530, 678
DpnII	GATC	5 cut(s)	321, 442, 494, 528, 676
DraI	TTTAAA	1 cut(s)	109
EaeI	YGGCCR	1 cut(s)	406
Eam1104I	CTCTTC	1 cut(s)	587
EarI	CTCTTC	1 cut(s)	587
Eco130I	CCWWGG	2 cut(s)	317, 409
Eco47I	GGWCC	4 cut(s)	76, 385, 413, 451
Eco57I	CTGAAG	1 cut(s)	470
Eco81I	CCTNAGG	1 cut(s)	447
EcoNI	CCTNNNNNAGG	1 cut(s)	539
EcoO109I	RGGNCCY	1 cut(s)	76
EcoRI	GAATTC	1 cut(s)	214
EcoRII	CCWGG	1 cut(s)	415
EcoT14I	CCWWGG	2 cut(s)	317, 409
ErhI	CCWWGG	2 cut(s)	317, 409
FaeI	CATG	5 cut(s)	321, 365, 413, 443, 473
FaqI	GGGAC	2 cut(s)	350, 466
FatI	CATG	5 cut(s)	317, 361, 409, 439, 469
FauNDI	CATATG	1 cut(s)	514
Fnu4HI	GCNGC	1 cut(s)	462
FokI	GGATG	2 cut(s)	432, 679
Fsp4HI	GCNGC	1 cut(s)	462
FspBI	CTAG	1 cut(s)	536
GlaI	GCGC	2 cut(s)	121, 136
GluI	GCNGC	1 cut(s)	462
HaeIII	GGCC	3 cut(s)	27, 393, 408
HhaI	GCGC	2 cut(s)	122, 137
Hin1II	CATG	5 cut(s)	321, 365, 413, 443, 473
Hin6I	GCGC	2 cut(s)	120, 135
HinP1I	GCGC	2 cut(s)	120, 135
HinfI	GANTC	2 cut(s)	94, 113
HphI	GGTGA	2 cut(s)	322, 601
Hpy188I	TCNGA	3 cut(s)	129, 385, 494
Hpy188III	TCNNGA	3 cut(s)	440, 446, 524
HpyAV	CCTTC	6 cut(s)	378, 382, 494, 580, 638, 642
HpyCH4V	TGCA	3 cut(s)	69, 340, 461
HpyF10VI	GCNNNNNNNGC	4 cut(s)	155, 272, 430, 470
HpyF3I	CTNAG	5 cut(s)	126, 300, 447, 540, 546
Hsp92II	CATG	5 cut(s)	321, 365, 413, 443, 473
HspAI	GCGC	2 cut(s)	120, 135
Kzo9I	GATC	5 cut(s)	321, 442, 494, 528, 676
LmnI	GCTCC	1 cut(s)	495
Lsp1109I	GCAGC	1 cut(s)	473
LweI	GCATC	2 cut(s)	262, 448
MaeI	CTAG	1 cut(s)	536
MalI	GATC	5 cut(s)	323, 444, 496, 530, 678
MboI	GATC	5 cut(s)	321, 442, 494, 528, 676
MboII	GAAGA	4 cut(s)	28, 372, 569, 604
MflI	RGATCY	2 cut(s)	528, 676
MlsI	TGGCCA	1 cut(s)	408
MluCI	AATT	3 cut(s)	214, 435, 499
MluNI	TGGCCA	1 cut(s)	408
MnlI	CCTC	8 cut(s)	50, 67, 90, 198, 398, 442, 542, 588
Mox20I	TGGCCA	1 cut(s)	408
MscI	TGGCCA	1 cut(s)	408
MseI	TTAA	3 cut(s)	108, 348, 396
MslI	CAYNNNNRTG	1 cut(s)	89
Msp20I	TGGCCA	1 cut(s)	408
MspR9I	CCNGG	1 cut(s)	417
MvaI	CCWGG	1 cut(s)	417
MvnI	CGCG	2 cut(s)	120, 137
MwoI	GCNNNNNNNGC	4 cut(s)	155, 272, 430, 470
NcoI	CCATGG	2 cut(s)	317, 409
NdeI	CATATG	1 cut(s)	514
NdeII	GATC	5 cut(s)	321, 442, 494, 528, 676
NlaIII	CATG	5 cut(s)	321, 365, 413, 443, 473
NlaIV	GGNNCC	1 cut(s)	78
NmeAIII	GCCGAG	1 cut(s)	369
PagI	TCATGA	1 cut(s)	439
PfeI	GAWTC	2 cut(s)	94, 113
PkrI	GCNGC	1 cut(s)	463
PpuMI	RGGWCCY	1 cut(s)	76
Psp5II	RGGWCCY	1 cut(s)	76
Psp6I	CCWGG	1 cut(s)	415
PspGI	CCWGG	1 cut(s)	415
PspN4I	GGNNCC	1 cut(s)	78
PspPI	GGNCC	4 cut(s)	76, 385, 413, 451
PspPPI	RGGWCCY	1 cut(s)	76
PsuI	RGATCY	2 cut(s)	528, 676
RsaI	GTAC	1 cut(s)	509
RsaNI	GTAC	1 cut(s)	508
RseI	CAYNNNNRTG	1 cut(s)	89
SaqAI	TTAA	3 cut(s)	108, 348, 396
SatI	GCNGC	1 cut(s)	462
Sau3AI	GATC	5 cut(s)	321, 442, 494, 528, 676
Sau96I	GGNCC	4 cut(s)	76, 385, 413, 451
ScrFI	CCNGG	1 cut(s)	417
SfaNI	GCATC	2 cut(s)	262, 448
SinI	GGWCC	4 cut(s)	76, 385, 413, 451
SmiMI	CAYNNNNRTG	1 cut(s)	89
SmlI	CTYRAG	2 cut(s)	194, 524
SmoI	CTYRAG	2 cut(s)	194, 524
Sse9I	AATT	3 cut(s)	214, 435, 499
SsiI	CCGC	1 cut(s)	137
SspMI	CTAG	1 cut(s)	536
StyD4I	CCNGG	1 cut(s)	415
StyI	CCWWGG	2 cut(s)	317, 409
TaqI	TCGA	1 cut(s)	659
TasI	AATT	3 cut(s)	214, 435, 499
TfiI	GAWTC	2 cut(s)	94, 113
Tru1I	TTAA	3 cut(s)	108, 348, 396
Tru9I	TTAA	3 cut(s)	108, 348, 396
TscAI	CASTG	1 cut(s)	435
TseI	GCWGC	1 cut(s)	461
TspDTI	ATGAA	6 cut(s)	17, 107, 309, 428, 531, 563
TspRI	CASTG	1 cut(s)	435
VpaK11BI	GGWCC	4 cut(s)	76, 385, 413, 451
XagI	CCTNNNNNAGG	1 cut(s)	539
XapI	RAATTY	2 cut(s)	214, 499
XspI	CTAG	1 cut(s)	536

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.