Rw4G017230

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0019324
Physical Location & Seq
Forward (+)
38806166 .. 38807362
1197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G017230.1

Sequence Viewer

Length: 1197 bp
ATGGATTCAAAATCAGAACTTGTAGCCGGAGTTGACGATAGGATGAGCCAATTACCAGATGAACTTATTTGTCACATACTTTCATTCCTTCCAACAGTAGAATCTGTGAAGACTATCGTTTTGTCTAAAAGATGGAACAAATTATGGAATTCCGTGAACATGTTAGACATCGACTACATCAGAGATTTTGGTGGAACATGCTATGTTGATTTTGTGAAATTTGTTGATGGTGCACTTTCAGTGCATGATTGGTCCAACATAAAAAAATTTCGTCTGTCTTGTCCCGGGGTCTTTTATGACGATGACGAACGTATTATCTATGTTATTCCGACATCTGGCTGTTTCTCAAGTCTCAAGTCCCTAAACCTTTTGCTACTTTATCAAGATTCTCCCAGCATTGAAAAGCTTATTACTAACTGCTGCCCTGTAGTTGAAGATCTGGAAATTCGTGGAGATATCAGGCTTCCACATGATATATCGATATTCAACATCTCTGCACCTAACTTGAAGAAGTTAACTGTGGATTTCGACCTATCCATTAGGGGTCGTTCGTCAAATGTTCTAATAAAAGCCCCAAAGCTTGAAAGCCTTTACTTCAAGAGGAAACACTTACCATATTCCGCTTATACATTGGAGAATCCAGAATCCCTAAACAGCGCAGTAATTGACGTCGCTGAGTGTCTGTCTGGGAGTGCTAACGGTTTTATGACTGAGCTTCTGGCCGGAATTTCTGGTGTTAAAGATTTGTACATTAGAGCTGCTTTTTTGGAGACCTGCAATCTCCCTGCAGATTTTAGTATACTGAACAAGTTGGAGTTAATTCTTTATCATAACTCCTATAGGGAATATGTAACCAAGTTTCTCAATAAGTCACCAAACCTAGAATATCTTGTCTTGGAGCTCGACAACAAATATCTACAAGAAGGGCAGTCTTTCACACCACCGGAGTTTGTGCCTGTTTGCTTGTCGTCGCACCTCAAGAAAATGTACATGAGGGGATTCAGTGGGCGGTGGGGGGAGTTGGAAGTGTTAAATTACTTGCTGCGGTACGGTGAAGTTTTGAATGAGTTGGCCATCTCTAGTCATCCAACTCTTGGCCATAGAAAAGAAGAATCATTGTACAAGGAAATATTACAATTTCCCCGGGCTTCGAGGACTTGTGAAGTTAAATTTTTGTCCAGCTTACTTTCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

398

Amino Acids

45.28

Weight (kDa)

5.72

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 16 - 53 1.3e-08 F-box domain
LRR_At1g61320_AtMIF1 PF23622 112 - 364 3.4e-08 At1g61320/AtMIF1, LRR domain
FBD PF08387 315 - 359 2.8e-09 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019324)

Species Orthologous Gene IDs
pyrus_communis pycom111g00870
rosa_chinensis RchiOBHm_Chr4g0416371
rosa_multiflora Rmu_sc0001488.1_g000007
rosa_rugosa Rorug04G0141400
rosa_samantha Rh4BG201000 Rh4CG215000 Rh4DG200900
rosa_wichuraiana Rw4G017230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 672
Acc36I ACCTGC 1 cut(s) 782
AccB7I CCANNNNNTGG 1 cut(s) 1094
AccI GTMKAC 1 cut(s) 799
AciI CCGC 3 cut(s) 621, 1009, 1045
AcoI YGGCCR 3 cut(s) 720, 1071, 1096
AcsI RAATTY 6 cut(s) 148, 218, 266, 444, 726, 1169
AcyI GRCGYC 1 cut(s) 669
AfaI GTAC 4 cut(s) 749, 989, 1049, 1121
AfiI CCNNNNNNNGG 2 cut(s) 335, 1094
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 8 cut(s) 9, 401, 434, 487, 508, 584, 598, 1063
AluBI AGCT 7 cut(s) 406, 580, 715, 758, 901, 1182, 1194
AluI AGCT 7 cut(s) 406, 580, 715, 758, 901, 1182, 1194
Alw21I GWGCWC 2 cut(s) 235, 903
Alw26I GTCTC 2 cut(s) 356, 764
Alw44I GTGCAC 1 cut(s) 231
Ama87I CYCGRG 2 cut(s) 284, 1143
AoxI GGCC 3 cut(s) 720, 1071, 1096
ApaLI GTGCAC 1 cut(s) 231
ApeKI GCWGC 3 cut(s) 420, 758, 1042
ApoI RAATTY 6 cut(s) 148, 218, 266, 444, 726, 1169
ArsI GACNNNNNNTTYG 2 cut(s) 103, 135
AspLEI GCGC 1 cut(s) 659
AspS9I GGNCC 1 cut(s) 252
AsuC2I CCSGG 4 cut(s) 285, 286, 1144, 1145
AsuHPI GGTGA 2 cut(s) 864, 1064
AvaI CYCGRG 2 cut(s) 284, 1143
AvaII GGWCC 1 cut(s) 252
BaeGI GKGCMC 1 cut(s) 235
BalI TGGCCA 2 cut(s) 1073, 1098
BanII GRGCYC 1 cut(s) 903
BbsI GAAGAC 1 cut(s) 116
Bbv12I GWGCWC 2 cut(s) 235, 903
BbvI GCAGC 3 cut(s) 407, 745, 1029
BccI CCATC 3 cut(s) 126, 221, 1082
BcnI CCSGG 4 cut(s) 285, 286, 1144, 1145
BcoDI GTCTC 2 cut(s) 356, 764
BfaI CTAG 4 cut(s) 881, 1080, 1191, 1195
BfmI CTRYAG 3 cut(s) 426, 786, 838
BfuAI ACCTGC 1 cut(s) 782
BglII AGATCT 1 cut(s) 436
BisI GCNGC 3 cut(s) 421, 759, 1043
BlsI GCNGC 3 cut(s) 422, 760, 1044
Bme1390I CCNGG 4 cut(s) 285, 286, 1144, 1145
Bme18I GGWCC 1 cut(s) 252
BmeT110I CYCGRG 2 cut(s) 284, 1143
BmgT120I GGNCC 1 cut(s) 252
BmrFI CCNGG 4 cut(s) 285, 286, 1144, 1145
BpiI GAAGAC 1 cut(s) 116
BpuEI CTTGAG 3 cut(s) 331, 338, 962
BpuMI CCSGG 4 cut(s) 285, 286, 1144, 1145
Bsa29I ATCGAT 1 cut(s) 479
BsaHI GRCGYC 1 cut(s) 669
BsaI GGTCTC 1 cut(s) 764
BsaJI CCNNGG 4 cut(s) 284, 285, 1142, 1143
BsaWI WCCGGW 1 cut(s) 943
Bsc4I CCNNNNNNNGG 2 cut(s) 335, 1094
BseCI ATCGAT 1 cut(s) 479
BseDI CCNNGG 4 cut(s) 284, 285, 1142, 1143
BseGI GGATG 2 cut(s) 48, 1084
BseLI CCNNNNNNNGG 2 cut(s) 335, 1094
BseMII CTCAG 2 cut(s) 666, 702
BseSI GKGCMC 1 cut(s) 235
BseXI GCAGC 3 cut(s) 407, 745, 1029
BseYI CCCAGC 1 cut(s) 392
BsgI GTGCAG 1 cut(s) 480
BshFI GGCC 3 cut(s) 722, 1073, 1098
BshVI ATCGAT 1 cut(s) 479
BsiHKAI GWGCWC 2 cut(s) 235, 903
BsiHKCI CYCGRG 2 cut(s) 284, 1143
BsiSI CCGG 5 cut(s) 27, 285, 723, 944, 1144
BslFI GGGAC 2 cut(s) 267, 343
BslI CCNNNNNNNGG 2 cut(s) 335, 1094
BsmAI GTCTC 2 cut(s) 356, 764
BsmFI GGGAC 2 cut(s) 267, 343
BsnI GGCC 3 cut(s) 722, 1073, 1098
Bso31I GGTCTC 1 cut(s) 764
BsoBI CYCGRG 2 cut(s) 284, 1143
Bsp1286I GDGCHC 2 cut(s) 235, 903
Bsp1407I TGTACA 3 cut(s) 747, 987, 1119
Bsp143I GATC 1 cut(s) 436
BspACI CCGC 3 cut(s) 621, 1009, 1045
BspANI GGCC 3 cut(s) 722, 1073, 1098
BspCNI CTCAG 2 cut(s) 667, 703
BspDI ATCGAT 1 cut(s) 479
BspMAI CTGCAG 1 cut(s) 790
BspMI ACCTGC 1 cut(s) 782
BspTNI GGTCTC 1 cut(s) 764
BsrGI TGTACA 3 cut(s) 747, 987, 1119
BssECI CCNNGG 4 cut(s) 284, 285, 1142, 1143
BssMI GATC 1 cut(s) 436
BssNAI GTATAC 1 cut(s) 800
BssNI GRCGYC 1 cut(s) 669
Bst1107I GTATAC 1 cut(s) 800
Bst4CI ACNGT 4 cut(s) 97, 520, 701, 1052
BstACI GRCGYC 1 cut(s) 669
BstAUI TGTACA 3 cut(s) 747, 987, 1119
BstDEI CTNAG 2 cut(s) 675, 711
BstF5I GGATG 2 cut(s) 48, 1084
BstHHI GCGC 1 cut(s) 659
BstKTI GATC 1 cut(s) 439
BstMAI GTCTC 2 cut(s) 356, 764
BstMBI GATC 1 cut(s) 436
BstNSI RCATGY 2 cut(s) 163, 201
BstSCI CCNGG 4 cut(s) 283, 284, 1142, 1143
BstSFI CTRYAG 3 cut(s) 426, 786, 838
BstSLI GKGCMC 1 cut(s) 235
BstV1I GCAGC 3 cut(s) 407, 745, 1029
BstV2I GAAGAC 1 cut(s) 116
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
BstZ17I GTATAC 1 cut(s) 800
Bsu15I ATCGAT 1 cut(s) 479
BsuRI GGCC 3 cut(s) 722, 1073, 1098
BsuTUI ATCGAT 1 cut(s) 479
BtsCI GGATG 2 cut(s) 48, 1084
BtsIMutI CAGTG 2 cut(s) 246, 1009
BveI ACCTGC 1 cut(s) 782
CfoI GCGC 1 cut(s) 659
Cfr13I GGNCC 1 cut(s) 252
Cfr9I CCCGGG 2 cut(s) 284, 1143
ClaI ATCGAT 1 cut(s) 479
Csp6I GTAC 4 cut(s) 748, 988, 1048, 1120
CviAII CATG 5 cut(s) 160, 198, 245, 470, 991
CviQI GTAC 4 cut(s) 748, 988, 1048, 1120
DdeI CTNAG 2 cut(s) 675, 711
DpnI GATC 1 cut(s) 438
DpnII GATC 1 cut(s) 436
EaeI YGGCCR 3 cut(s) 720, 1071, 1096
Ecl136II GAGCTC 1 cut(s) 901
Eco24I GRGCYC 1 cut(s) 903
Eco31I GGTCTC 1 cut(s) 764
Eco32I GATATC 1 cut(s) 457
Eco47I GGWCC 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 901
Eco88I CYCGRG 2 cut(s) 284, 1143
EcoICRI GAGCTC 1 cut(s) 901
EcoRI GAATTC 1 cut(s) 148
EcoRV GATATC 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 903
FaeI CATG 5 cut(s) 163, 201, 248, 473, 994
FaqI GGGAC 2 cut(s) 267, 343
FatI CATG 5 cut(s) 159, 197, 244, 469, 990
FblI GTMKAC 1 cut(s) 799
Fnu4HI GCNGC 3 cut(s) 421, 759, 1043
FokI GGATG 2 cut(s) 55, 1071
FriOI GRGCYC 1 cut(s) 903
Fsp4HI GCNGC 3 cut(s) 421, 759, 1043
FspBI CTAG 4 cut(s) 881, 1080, 1191, 1195
GlaI GCGC 1 cut(s) 658
GluI GCNGC 3 cut(s) 421, 759, 1043
GsaI CCCAGC 1 cut(s) 396
HaeIII GGCC 3 cut(s) 722, 1073, 1098
HapII CCGG 5 cut(s) 27, 285, 723, 944, 1144
HhaI GCGC 1 cut(s) 659
Hin1I GRCGYC 1 cut(s) 669
Hin1II CATG 5 cut(s) 163, 201, 248, 473, 994
Hin6I GCGC 1 cut(s) 657
HinP1I GCGC 1 cut(s) 657
HincII GTYRAC 2 cut(s) 34, 516
HindII GTYRAC 2 cut(s) 34, 516
HindIII AAGCTT 2 cut(s) 404, 578
HinfI GANTC 7 cut(s) 5, 101, 386, 637, 644, 999, 1112
HpaI GTTAAC 1 cut(s) 516
HpaII CCGG 5 cut(s) 27, 285, 723, 944, 1144
HphI GGTGA 2 cut(s) 864, 1064
Hpy166II GTNNAC 5 cut(s) 34, 157, 233, 516, 800
Hpy188I TCNGA 3 cut(s) 16, 182, 330
Hpy188III TCNNGA 5 cut(s) 383, 440, 598, 641, 979
Hpy8I GTNNAC 5 cut(s) 34, 157, 233, 516, 800
Hpy99I CGWCG 2 cut(s) 674, 973
HpyAV CCTTC 2 cut(s) 98, 917
HpyCH4III ACNGT 4 cut(s) 97, 520, 701, 1052
HpyCH4IV ACGT 2 cut(s) 310, 669
HpyCH4V TGCA 5 cut(s) 233, 244, 497, 777, 788
HpyF3I CTNAG 2 cut(s) 675, 711
HpySE526I ACGT 2 cut(s) 310, 669
Hsp92I GRCGYC 1 cut(s) 669
Hsp92II CATG 5 cut(s) 163, 201, 248, 473, 994
HspAI GCGC 1 cut(s) 657
KspAI GTTAAC 1 cut(s) 516
Kzo9I GATC 1 cut(s) 436
LmnI GCTCC 1 cut(s) 898
Lsp1109I GCAGC 3 cut(s) 407, 745, 1029
MaeI CTAG 4 cut(s) 881, 1080, 1191, 1195
MaeII ACGT 2 cut(s) 310, 669
MaeIII GTNAC 3 cut(s) 71, 850, 870
MalI GATC 1 cut(s) 438
MboI GATC 1 cut(s) 436
MboII GAAGA 4 cut(s) 121, 446, 520, 1121
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 2 cut(s) 235, 903
MlsI TGGCCA 2 cut(s) 1073, 1098
MluNI TGGCCA 2 cut(s) 1073, 1098
MmeI TCCRAC 6 cut(s) 116, 279, 353, 792, 1002, 1112
MnlI CCTC 4 cut(s) 594, 986, 987, 1146
Mox20I TGGCCA 2 cut(s) 1073, 1098
MscI TGGCCA 2 cut(s) 1073, 1098
MseI TTAA 5 cut(s) 515, 738, 818, 1031, 1167
Msp20I TGGCCA 2 cut(s) 1073, 1098
MspI CCGG 5 cut(s) 27, 285, 723, 944, 1144
MspR9I CCNGG 4 cut(s) 285, 286, 1144, 1145
NciI CCSGG 4 cut(s) 285, 286, 1144, 1145
NdeII GATC 1 cut(s) 436
NlaIII CATG 5 cut(s) 163, 201, 248, 473, 994
NmuCI GTSAC 2 cut(s) 71, 870
NspI RCATGY 2 cut(s) 163, 201
PciI ACATGT 1 cut(s) 159
PfeI GAWTC 7 cut(s) 5, 101, 386, 637, 644, 999, 1112
PflMI CCANNNNNTGG 1 cut(s) 1094
PkrI GCNGC 3 cut(s) 422, 760, 1044
PscI ACATGT 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 903
PspFI CCCAGC 1 cut(s) 392
PspPI GGNCC 1 cut(s) 252
PstI CTGCAG 1 cut(s) 790
PsuI RGATCY 1 cut(s) 436
RsaI GTAC 4 cut(s) 749, 989, 1049, 1121
RsaNI GTAC 4 cut(s) 748, 988, 1048, 1120
SacI GAGCTC 1 cut(s) 903
SaqAI TTAA 5 cut(s) 515, 738, 818, 1031, 1167
SatI GCNGC 3 cut(s) 421, 759, 1043
Sau3AI GATC 1 cut(s) 436
Sau96I GGNCC 1 cut(s) 252
ScrFI CCNGG 4 cut(s) 285, 286, 1144, 1145
SduI GDGCHC 2 cut(s) 235, 903
SfcI CTRYAG 3 cut(s) 426, 786, 838
SinI GGWCC 1 cut(s) 252
SmaI CCCGGG 2 cut(s) 286, 1145
SmlI CTYRAG 3 cut(s) 346, 353, 977
SmoI CTYRAG 3 cut(s) 346, 353, 977
SsiI CCGC 3 cut(s) 621, 1009, 1045
SspI AATATT 1 cut(s) 1131
SspMI CTAG 4 cut(s) 881, 1080, 1191, 1195
SstI GAGCTC 1 cut(s) 903
StyD4I CCNGG 4 cut(s) 283, 284, 1142, 1143
TaaI ACNGT 4 cut(s) 97, 520, 701, 1052
TaiI ACGT 2 cut(s) 313, 672
TaqI TCGA 5 cut(s) 171, 479, 528, 903, 1151
TatI WGTACW 3 cut(s) 747, 987, 1119
TfiI GAWTC 7 cut(s) 5, 101, 386, 637, 644, 999, 1112
Tru1I TTAA 5 cut(s) 515, 738, 818, 1031, 1167
Tru9I TTAA 5 cut(s) 515, 738, 818, 1031, 1167
TscAI CASTG 2 cut(s) 246, 1009
TseFI GTSAC 2 cut(s) 71, 870
TseI GCWGC 3 cut(s) 420, 758, 1042
Tsp45I GTSAC 2 cut(s) 71, 870
TspDTI ATGAA 2 cut(s) 72, 75
TspGWI ACGGA 1 cut(s) 142
TspMI CCCGGG 2 cut(s) 284, 1143
TspRI CASTG 2 cut(s) 246, 1009
Van91I CCANNNNNTGG 1 cut(s) 1094
VneI GTGCAC 1 cut(s) 231
VpaK11BI GGWCC 1 cut(s) 252
XapI RAATTY 6 cut(s) 148, 218, 266, 444, 726, 1169
XceI RCATGY 2 cut(s) 163, 201
XmaI CCCGGG 2 cut(s) 284, 1143
XmiI GTMKAC 1 cut(s) 799
XspI CTAG 4 cut(s) 881, 1080, 1191, 1195
ZraI GACGTC 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.