Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0015146

Physical Location & Seq

Genomic Coordinates Forward (+)

60953687 .. 60955387

Gene Structure 1701 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G035280.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1368 bp

ATGTTGGAACAGAAGAGAGCCCCGAAGAGCCGGGGCTTCTACGTCAGAATGAAGCTCCAGAGCAGCAAACATGGCCGGCCTCAAGAAAAGAGCTCCTTCTACTCATATTGCAAATGGGTCCTCTGGCTTTCCCTCACCCTTTACTTCTTCAGCTCCTACCTCATTAGTAACCACCCAAATCAGAACAAGCCCACCACCTCTCTCTCCAAAGCCCATTTCTCGCCCTCAGCCTCCCGCGCCCTTTTCGAATCCGCCATTGACAACACCACCGCCGTCCTCGAAAACAAAGGACTGTTCAAAGGGTTGAAGGTTTTCGTGTACGACTTGCCGGCGAAATACAACGCGGATTGGCTCTCCAACGATCGGTGCAGAACCCACTTATTCGCCTCCGAAGTGGCAATTCATCGGGCGCTGTTGACCAGCGACTTTTTGACCCTTGACCCTTCCCAAGCCGACTTCTTCTTCGTTCCCGTCTACGTGTCCTGTAATTTCAGCACCGTCAATGGATTCCCAGCCATTGGTCACGCCCGGACCCTCATCGCCTCCGCCATCCAGCTCATCAGGACGCGGTACCCGTTTTGGGACCGCACCCAAGGCTCCGACCACGTCTTTGTCGCTTCCCACGACTTCGGTTCTTGCTTTCACACAATGGAGAATGTGGCTATCGCAGATGGAGTGCCCGATTTTCTGAAGAAATCGATCGTTTTGCAGACGTTTGGGGTGAAGCACAAGCACCCATGTCAAGAGGTTGAGAATGTGGTCATTCCGCCGTACGTTTCGCCGGAGAGTGTACGGACGACCCTGGAGAATGCTCCGGCGGACGGCCGGCGGGACATCTTCGCATTCTTCCGGGGAAAGATGGAGCTGCACCCGAAAAACGTCAGCGGACGATATTACAGCAAGAGGGTGCGGACGAATATATGGCGGAAGTTCAACGGCGACCGGAGGTTTTACCTGCAACGCAGTCGTTTTGCTGGTTATCAGTCGGAAATTCTAAGGTCGGTTTTCTGTTTGTGCCCGCTTGGGTGGGCCCCGTGGAGTCCGAGACTGGTTGAGTCAGTTGCGCTGGGGTGTGTACCAGTGATAATAGCGGACGGGATAAGGCTGCCCTTCGACGACGTCGTTCCGTGGGCGGAGATTTCGCTAACCGTGGCGGAGAGAGACGTGGGTAAATTGGGAAATATACTCGAACACGTGGCGGCGACGAACCTGAGCGCTATACAAAAGAACATATGGGACCCAAGGGTGAGTCGGGCCCTGCTGTTTCACGATCGGATCCAACCAGGCGACGCCACGTGGCAGGTTCTGTCCGCCCTGGAGAACAAGCTGGCGAGGTCTTACAGGCGCGTGAGTATTTCCAGCGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009059 GO:0009832 GO:0009834 GO:0009987 GO:0010383 GO:0010410 GO:0010413 GO:0010417 GO:0012505 GO:0015020 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045492 GO:0070589 GO:0070592 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Exostosin

Protein Analysis

455

Amino Acids

51.75

Weight (kDa)

9.53

Isoelectric Point (pI)

45.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exostosin_GT47	PF03016	100 - 398	1.3e-75	Exostosin GT47 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015146)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28110
fragaria_vesca	FvH4_3g30840
malus_domestica	MD03G1126500.v1.1 MD11G1146800.v1.1
prunus_persica	Prupe.6G110800_v2.0.a1
pyrus_communis	pycom11g12010
rosa_chinensis	RchiOBHm_Chr5g0057051
rosa_laevigata	RLG00000035148
rosa_multiflora	Rmu_sc0009185.1_g000025
rosa_roxburghii	Rroxscaffold_1G00023140
rosa_rugosa	Rorug05G0305300.1 Rorug05G0305400
rosa_samantha	Rh5BG385800 Rh5CG409300 Rh5DG399500
rosa_wichuraiana	Rw5G035280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1122
Acc36I	ACCTGC	2 cut(s)	963, 1291
Acc65I	GGTACC	1 cut(s)	570
AccB1I	GGYRCC	1 cut(s)	570
AccB7I	CCANNNNNTGG	1 cut(s)	518
AccI	GTMKAC	1 cut(s)	474
AccII	CGCG	4 cut(s)	237, 344, 568, 1347
AclWI	GGATC	2 cut(s)	1270, 1283
AcoI	YGGCCR	2 cut(s)	73, 823
AcsI	RAATTY	1 cut(s)	990
AcuI	CTGAAG	2 cut(s)	133, 710
AcvI	CACGTG	2 cut(s)	1195, 1296
AcyI	GRCGYC	2 cut(s)	1119, 1290
AfaI	GTAC	5 cut(s)	320, 572, 773, 792, 1077
AfeI	AGCGCT	1 cut(s)	1216
AfiI	CCNNNNNNNGG	4 cut(s)	363, 518, 580, 821
AflIII	ACRYGT	2 cut(s)	477, 1192
AgsI	TTSAA	3 cut(s)	298, 307, 934
AjiI	CACGTC	2 cut(s)	607, 1165
AjnI	CCWGG	3 cut(s)	801, 1282, 1314
AjuI	GAANNNNNNNTTGG	2 cut(s)	200, 232
AluBI	AGCT	6 cut(s)	55, 93, 153, 556, 865, 1327
AluI	AGCT	6 cut(s)	55, 93, 153, 556, 865, 1327
Alw21I	GWGCWC	1 cut(s)	95
Alw26I	GTCTC	2 cut(s)	1039, 1155
AlwI	GGATC	2 cut(s)	1270, 1283
AlwNI	CAGNNNCTG	1 cut(s)	1306
Aor51HI	AGCGCT	1 cut(s)	1216
AoxI	GGCC	5 cut(s)	73, 77, 823, 1029, 1254
ApaI	GGGCCC	2 cut(s)	1033, 1258
ApeKI	GCWGC	3 cut(s)	63, 865, 1105
ApoI	RAATTY	1 cut(s)	990
ArsI	GACNNNNNNTTYG	4 cut(s)	446, 478, 593, 625
Asp700I	GAANNNNTTC	1 cut(s)	311
Asp718I	GGTACC	1 cut(s)	570
AspLEI	GCGC	5 cut(s)	239, 412, 1066, 1217, 1347
AspS9I	GGNCC	8 cut(s)	118, 531, 583, 1029, 1030, 1237, 1254, 1255
AsuC2I	CCSGG	3 cut(s)	32, 529, 851
AsuHPI	GGTGA	3 cut(s)	127, 733, 1258
AsuII	TTCGAA	1 cut(s)	246
AvaII	GGWCC	4 cut(s)	118, 531, 583, 1237
BaeGI	GKGCMC	4 cut(s)	681, 1019, 1033, 1258
BamHI	GGATCC	1 cut(s)	1275
BanI	GGYRCC	1 cut(s)	570
BanII	GRGCYC	4 cut(s)	22, 95, 1033, 1258
BbrPI	CACGTG	2 cut(s)	1195, 1296
Bbv12I	GWGCWC	1 cut(s)	95
BbvCI	CCTCAGC	1 cut(s)	226
BbvI	GCAGC	3 cut(s)	75, 852, 1092
BccI	CCATC	3 cut(s)	557, 665, 853
BceAI	ACGGC	4 cut(s)	257, 754, 838, 952
BcgI	CGANNNNNNTGC	4 cut(s)	688, 722, 947, 981
BciT130I	CCWGG	3 cut(s)	803, 1284, 1316
BcnI	CCSGG	3 cut(s)	32, 529, 851
BcoDI	GTCTC	2 cut(s)	1039, 1155
BfoI	RGCGCY	2 cut(s)	413, 1218
BfuAI	ACCTGC	2 cut(s)	963, 1291
BisI	GCNGC	4 cut(s)	64, 866, 1106, 1200
BlsI	GCNGC	4 cut(s)	65, 867, 1107, 1201
Bme1390I	CCNGG	6 cut(s)	32, 529, 803, 851, 1284, 1316
Bme18I	GGWCC	4 cut(s)	118, 531, 583, 1237
BmgBI	CACGTC	2 cut(s)	607, 1165
BmgT120I	GGNCC	8 cut(s)	118, 531, 583, 1029, 1030, 1237, 1254, 1255
BmrFI	CCNGG	6 cut(s)	32, 529, 803, 851, 1284, 1316
BpmI	CTGGAG	3 cut(s)	41, 824, 1337
Bpu10I	CCTNAGC	2 cut(s)	226, 1211
Bpu14I	TTCGAA	1 cut(s)	246
BpuEI	CTTGAG	1 cut(s)	66
BpuMI	CCSGG	3 cut(s)	32, 529, 851
Bsa29I	ATCGAT	1 cut(s)	698
BsaAI	YACGTR	3 cut(s)	478, 1195, 1296
BsaHI	GRCGYC	2 cut(s)	1119, 1290
BsaJI	CCNNGG	9 cut(s)	31, 592, 801, 850, 1034, 1127, 1149, 1241, 1314
BsaWI	WCCGGW	1 cut(s)	942
Bsc4I	CCNNNNNNNGG	4 cut(s)	363, 518, 580, 821
Bse118I	RCCGGY	3 cut(s)	75, 328, 825
Bse1I	ACTGG	2 cut(s)	1053, 1079
BseBI	CCWGG	3 cut(s)	803, 1284, 1316
BseCI	ATCGAT	1 cut(s)	698
BseDI	CCNNGG	9 cut(s)	31, 592, 801, 850, 1034, 1127, 1149, 1241, 1314
BseGI	GGATG	1 cut(s)	549
BseLI	CCNNNNNNNGG	4 cut(s)	363, 518, 580, 821
BseMII	CTCAG	2 cut(s)	240, 1202
BseNI	ACTGG	2 cut(s)	1053, 1079
BseSI	GKGCMC	4 cut(s)	681, 1019, 1033, 1258
BseX3I	CGGCCG	1 cut(s)	823
BseXI	GCAGC	3 cut(s)	75, 852, 1092
BseYI	CCCAGC	2 cut(s)	511, 1066
BsgI	GTGCAG	2 cut(s)	388, 851
Bsh1236I	CGCG	4 cut(s)	237, 344, 568, 1347
Bsh1285I	CGRYCG	5 cut(s)	364, 702, 826, 943, 1273
BshFI	GGCC	5 cut(s)	75, 79, 825, 1031, 1256
BshNI	GGYRCC	1 cut(s)	570
BshVI	ATCGAT	1 cut(s)	698
BsiEI	CGRYCG	5 cut(s)	364, 702, 826, 943, 1273
BsiHKAI	GWGCWC	1 cut(s)	95
BsiSI	CCGG	9 cut(s)	31, 76, 329, 529, 782, 815, 826, 850, 943
BsiWI	CGTACG	1 cut(s)	771
BslFI	GGGAC	3 cut(s)	596, 845, 1250
BslI	CCNNNNNNNGG	4 cut(s)	363, 518, 580, 821
BsmAI	GTCTC	2 cut(s)	1039, 1155
BsmBI	CGTCTC	1 cut(s)	1155
BsmFI	GGGAC	3 cut(s)	596, 845, 1250
BsmI	GAATGC	2 cut(s)	814, 842
BsnI	GGCC	5 cut(s)	75, 79, 825, 1031, 1256
Bsp119I	TTCGAA	1 cut(s)	246
Bsp120I	GGGCCC	2 cut(s)	1029, 1254
Bsp1286I	GDGCHC	6 cut(s)	22, 95, 681, 1019, 1033, 1258
Bsp143I	GATC	4 cut(s)	361, 699, 1270, 1275
BspANI	GGCC	5 cut(s)	75, 79, 825, 1031, 1256
BspCNI	CTCAG	2 cut(s)	239, 1203
BspDI	ATCGAT	1 cut(s)	698
BspFNI	CGCG	4 cut(s)	237, 344, 568, 1347
BspMI	ACCTGC	2 cut(s)	963, 1291
BspPI	GGATC	2 cut(s)	1270, 1283
BspQI	GCTCTTC	1 cut(s)	20
BspT104I	TTCGAA	1 cut(s)	246
BspT107I	GGYRCC	1 cut(s)	570
BsrFI	RCCGGY	3 cut(s)	75, 328, 825
BsrI	ACTGG	2 cut(s)	1053, 1079
BssAI	RCCGGY	3 cut(s)	75, 328, 825
BssECI	CCNNGG	9 cut(s)	31, 592, 801, 850, 1034, 1127, 1149, 1241, 1314
BssMI	GATC	4 cut(s)	361, 699, 1270, 1275
BssNI	GRCGYC	2 cut(s)	1119, 1290
BssT1I	CCWWGG	2 cut(s)	592, 1241
Bst2UI	CCWGG	3 cut(s)	803, 1284, 1316
Bst4CI	ACNGT	3 cut(s)	294, 499, 1150
Bst6I	CTCTTC	2 cut(s)	8, 20
BstACI	GRCGYC	2 cut(s)	1119, 1290
BstBAI	YACGTR	3 cut(s)	478, 1195, 1296
BstBI	TTCGAA	1 cut(s)	246
BstC8I	GCNNGC	5 cut(s)	77, 330, 827, 1019, 1329
BstDEI	CTNAG	3 cut(s)	226, 995, 1211
BstDSI	CCRYGG	3 cut(s)	1034, 1127, 1149
BstF5I	GGATG	1 cut(s)	549
BstFNI	CGCG	4 cut(s)	237, 344, 568, 1347
BstH2I	RGCGCY	2 cut(s)	413, 1218
BstHHI	GCGC	5 cut(s)	239, 412, 1066, 1217, 1347
BstKTI	GATC	4 cut(s)	364, 702, 1273, 1278
BstMAI	GTCTC	2 cut(s)	1039, 1155
BstMBI	GATC	4 cut(s)	361, 699, 1270, 1275
BstMCI	CGRYCG	5 cut(s)	364, 702, 826, 943, 1273
BstMWI	GCNNNNNNNGC	3 cut(s)	72, 236, 594
BstNI	CCWGG	3 cut(s)	803, 1284, 1316
BstSCI	CCNGG	6 cut(s)	30, 527, 801, 849, 1282, 1314
BstSLI	GKGCMC	4 cut(s)	681, 1019, 1033, 1258
BstUI	CGCG	4 cut(s)	237, 344, 568, 1347
BstV1I	GCAGC	3 cut(s)	75, 852, 1092
BstX2I	RGATCY	1 cut(s)	1275
BstYI	RGATCY	1 cut(s)	1275
BstZI	CGGCCG	1 cut(s)	823
Bsu15I	ATCGAT	1 cut(s)	698
BsuRI	GGCC	5 cut(s)	75, 79, 825, 1031, 1256
BsuTUI	ATCGAT	1 cut(s)	698
BtgI	CCRYGG	3 cut(s)	1034, 1127, 1149
BtgZI	GCGATG	1 cut(s)	523
BtrI	CACGTC	2 cut(s)	607, 1165
BtsCI	GGATG	1 cut(s)	549
BtsIMutI	CAGTG	1 cut(s)	1086
BveI	ACCTGC	2 cut(s)	963, 1291
Cac8I	GCNNGC	5 cut(s)	77, 330, 827, 1019, 1329
CaiI	CAGNNNCTG	1 cut(s)	1306
CfoI	GCGC	5 cut(s)	239, 412, 1066, 1217, 1347
Cfr10I	RCCGGY	3 cut(s)	75, 328, 825
Cfr13I	GGNCC	8 cut(s)	118, 531, 583, 1029, 1030, 1237, 1254, 1255
ClaI	ATCGAT	1 cut(s)	698
CseI	GACGC	2 cut(s)	574, 1298
Csp6I	GTAC	5 cut(s)	319, 571, 772, 791, 1076
CviAII	CATG	2 cut(s)	71, 738
CviQI	GTAC	5 cut(s)	319, 571, 772, 791, 1076
DdeI	CTNAG	3 cut(s)	226, 995, 1211
DpnI	GATC	4 cut(s)	363, 701, 1272, 1277
DpnII	GATC	4 cut(s)	361, 699, 1270, 1275
EaeI	YGGCCR	2 cut(s)	73, 823
EagI	CGGCCG	1 cut(s)	823
Eam1104I	CTCTTC	2 cut(s)	8, 20
EarI	CTCTTC	2 cut(s)	8, 20
EciI	GGCGGA	8 cut(s)	241, 535, 756, 833, 940, 1148, 1169, 1300
Ecl136II	GAGCTC	1 cut(s)	93
EclXI	CGGCCG	1 cut(s)	823
Eco130I	CCWWGG	2 cut(s)	592, 1241
Eco24I	GRGCYC	4 cut(s)	22, 95, 1033, 1258
Eco47I	GGWCC	4 cut(s)	118, 531, 583, 1237
Eco47III	AGCGCT	1 cut(s)	1216
Eco52I	CGGCCG	1 cut(s)	823
Eco53kI	GAGCTC	1 cut(s)	93
Eco57I	CTGAAG	2 cut(s)	133, 710
Eco72I	CACGTG	2 cut(s)	1195, 1296
EcoICRI	GAGCTC	1 cut(s)	93
EcoO109I	RGGNCCY	4 cut(s)	118, 1030, 1237, 1255
EcoRII	CCWGG	3 cut(s)	801, 1282, 1314
EcoT14I	CCWWGG	2 cut(s)	592, 1241
EcoT38I	GRGCYC	4 cut(s)	22, 95, 1033, 1258
ErhI	CCWWGG	2 cut(s)	592, 1241
Esp3I	CGTCTC	1 cut(s)	1155
FaeI	CATG	2 cut(s)	74, 741
FaiI	YATR	9 cut(s)	72, 106, 739, 920, 922, 1184, 1220, 1232, 1234
FalI	AAGNNNNNCTT	2 cut(s)	80, 112
FaqI	GGGAC	3 cut(s)	596, 845, 1250
FatI	CATG	2 cut(s)	70, 737
FauI	CCCGC	3 cut(s)	242, 822, 1026
FauNDI	CATATG	1 cut(s)	1232
FblI	GTMKAC	1 cut(s)	474
Fnu4HI	GCNGC	4 cut(s)	64, 866, 1106, 1200
FokI	GGATG	1 cut(s)	536
FriOI	GRGCYC	4 cut(s)	22, 95, 1033, 1258
FseI	GGCCGGCC	1 cut(s)	79
Fsp4HI	GCNGC	4 cut(s)	64, 866, 1106, 1200
GlaI	GCGC	5 cut(s)	238, 411, 1065, 1216, 1346
GluI	GCNGC	4 cut(s)	64, 866, 1106, 1200
GsaI	CCCAGC	2 cut(s)	515, 1070
GsuI	CTGGAG	3 cut(s)	41, 824, 1337
HaeII	RGCGCY	2 cut(s)	413, 1218
HaeIII	GGCC	5 cut(s)	75, 79, 825, 1031, 1256
HapII	CCGG	9 cut(s)	31, 76, 329, 529, 782, 815, 826, 850, 943
HgaI	GACGC	2 cut(s)	574, 1298
HhaI	GCGC	5 cut(s)	239, 412, 1066, 1217, 1347
Hin1I	GRCGYC	2 cut(s)	1119, 1290
Hin1II	CATG	2 cut(s)	74, 741
Hin6I	GCGC	5 cut(s)	237, 410, 1064, 1215, 1345
HinP1I	GCGC	5 cut(s)	237, 410, 1064, 1215, 1345
HincII	GTYRAC	1 cut(s)	417
HindII	GTYRAC	1 cut(s)	417
HinfI	GANTC	5 cut(s)	248, 507, 1039, 1055, 1249
HpaII	CCGG	9 cut(s)	31, 76, 329, 529, 782, 815, 826, 850, 943
HphI	GGTGA	3 cut(s)	127, 733, 1258
Hpy166II	GTNNAC	5 cut(s)	319, 417, 475, 791, 1076
Hpy188I	TCNGA	8 cut(s)	47, 183, 391, 601, 690, 988, 1044, 1275
Hpy188III	TCNNGA	5 cut(s)	58, 83, 562, 743, 1268
Hpy8I	GTNNAC	5 cut(s)	319, 417, 475, 791, 1076
Hpy99I	CGWCG	5 cut(s)	1118, 1121, 1124, 1207, 1292
HpyAV	CCTTC	4 cut(s)	106, 301, 453, 1120
HpyCH4III	ACNGT	3 cut(s)	294, 499, 1150
HpyCH4V	TGCA	5 cut(s)	111, 369, 709, 868, 958
HpyF10VI	GCNNNNNNNGC	3 cut(s)	72, 236, 594
HpyF3I	CTNAG	3 cut(s)	226, 995, 1211
Hsp92I	GRCGYC	2 cut(s)	1119, 1290
Hsp92II	CATG	2 cut(s)	74, 741
HspAI	GCGC	5 cut(s)	237, 410, 1064, 1215, 1345
KflI	GGGWCCC	1 cut(s)	1237
KpnI	GGTACC	1 cut(s)	574
KroI	GCCGGC	3 cut(s)	75, 328, 825
KroNI	GCCGGC	3 cut(s)	77, 330, 827
Kzo9I	GATC	4 cut(s)	361, 699, 1270, 1275
LguI	GCTCTTC	1 cut(s)	20
LmnI	GCTCC	6 cut(s)	60, 98, 158, 602, 817, 862
Lsp1109I	GCAGC	3 cut(s)	75, 852, 1092
MaeIII	GTNAC	2 cut(s)	167, 521
MalI	GATC	4 cut(s)	363, 701, 1272, 1277
MboI	GATC	4 cut(s)	361, 699, 1270, 1275
MboII	GAAGA	8 cut(s)	25, 37, 139, 451, 454, 703, 829, 838
MflI	RGATCY	1 cut(s)	1275
MhlI	GDGCHC	6 cut(s)	22, 95, 681, 1019, 1033, 1258
MluCI	AATT	4 cut(s)	399, 487, 990, 1172
MlyI	GAGTC	3 cut(s)	1048, 1064, 1258
MmeI	TCCRAC	4 cut(s)	381, 624, 966, 1303
MroNI	GCCGGC	3 cut(s)	75, 328, 825
MroXI	GAANNNNTTC	1 cut(s)	311
MspA1I	CMGCKG	1 cut(s)	885
MspI	CCGG	9 cut(s)	31, 76, 329, 529, 782, 815, 826, 850, 943
MspR9I	CCNGG	6 cut(s)	32, 529, 803, 851, 1284, 1316
Mva1269I	GAATGC	2 cut(s)	814, 842
MvaI	CCWGG	3 cut(s)	803, 1284, 1316
MvnI	CGCG	4 cut(s)	237, 344, 568, 1347
MwoI	GCNNNNNNNGC	3 cut(s)	72, 236, 594
NaeI	GCCGGC	3 cut(s)	77, 330, 827
NciI	CCSGG	3 cut(s)	32, 529, 851
NdeI	CATATG	1 cut(s)	1232
NdeII	GATC	4 cut(s)	361, 699, 1270, 1275
NgoMIV	GCCGGC	3 cut(s)	75, 328, 825
NlaIII	CATG	2 cut(s)	74, 741
NmuCI	GTSAC	1 cut(s)	521
NspV	TTCGAA	1 cut(s)	246
PciSI	GCTCTTC	1 cut(s)	20
PcsI	WCGNNNNNNNCGW	3 cut(s)	572, 621, 1119
PctI	GAATGC	2 cut(s)	814, 842
PdiI	GCCGGC	3 cut(s)	77, 330, 827
PdmI	GAANNNNTTC	1 cut(s)	311
PfeI	GAWTC	2 cut(s)	248, 507
Pfl23II	CGTACG	1 cut(s)	771
PflFI	GACNNNGTC	2 cut(s)	605, 1118
PflMI	CCANNNNNTGG	1 cut(s)	518
PkrI	GCNGC	4 cut(s)	65, 867, 1107, 1201
Ple19I	CGATCG	3 cut(s)	364, 702, 1273
PleI	GAGTC	3 cut(s)	1047, 1063, 1257
PmaCI	CACGTG	2 cut(s)	1195, 1296
PmlI	CACGTG	2 cut(s)	1195, 1296
PpsI	GAGTC	3 cut(s)	1047, 1063, 1257
Ppu21I	YACGTR	3 cut(s)	478, 1195, 1296
PpuMI	RGGWCCY	2 cut(s)	118, 1237
Psp124BI	GAGCTC	1 cut(s)	95
Psp5II	RGGWCCY	2 cut(s)	118, 1237
Psp6I	CCWGG	3 cut(s)	801, 1282, 1314
PspCI	CACGTG	2 cut(s)	1195, 1296
PspFI	CCCAGC	2 cut(s)	511, 1066
PspGI	CCWGG	3 cut(s)	801, 1282, 1314
PspLI	CGTACG	1 cut(s)	771
PspOMI	GGGCCC	2 cut(s)	1029, 1254
PspPI	GGNCC	8 cut(s)	118, 531, 583, 1029, 1030, 1237, 1254, 1255
PspPPI	RGGWCCY	2 cut(s)	118, 1237
PstNI	CAGNNNCTG	1 cut(s)	1306
PsuI	RGATCY	1 cut(s)	1275
PsyI	GACNNNGTC	2 cut(s)	605, 1118
PvuI	CGATCG	3 cut(s)	364, 702, 1273
RigI	GGCCGGCC	1 cut(s)	79
RsaI	GTAC	5 cut(s)	320, 572, 773, 792, 1077
RsaNI	GTAC	5 cut(s)	319, 571, 772, 791, 1076
SacI	GAGCTC	1 cut(s)	95
SapI	GCTCTTC	1 cut(s)	20
SatI	GCNGC	4 cut(s)	64, 866, 1106, 1200
Sau3AI	GATC	4 cut(s)	361, 699, 1270, 1275
Sau96I	GGNCC	8 cut(s)	118, 531, 583, 1029, 1030, 1237, 1254, 1255
SchI	GAGTC	3 cut(s)	1048, 1064, 1258
ScrFI	CCNGG	6 cut(s)	32, 529, 803, 851, 1284, 1316
SduI	GDGCHC	6 cut(s)	22, 95, 681, 1019, 1033, 1258
SfuI	TTCGAA	1 cut(s)	246
SinI	GGWCC	4 cut(s)	118, 531, 583, 1237
SmlI	CTYRAG	1 cut(s)	81
SmoI	CTYRAG	1 cut(s)	81
Sse9I	AATT	4 cut(s)	399, 487, 990, 1172
SstI	GAGCTC	1 cut(s)	95
StyD4I	CCNGG	6 cut(s)	30, 527, 801, 849, 1282, 1314
StyI	CCWWGG	2 cut(s)	592, 1241
TaaI	ACNGT	3 cut(s)	294, 499, 1150
TaqI	TCGA	5 cut(s)	246, 279, 698, 1113, 1188
TasI	AATT	4 cut(s)	399, 487, 990, 1172
TauI	GCSGC	1 cut(s)	1202
TfiI	GAWTC	2 cut(s)	248, 507
TscAI	CASTG	1 cut(s)	1086
TseFI	GTSAC	1 cut(s)	521
TseI	GCWGC	3 cut(s)	63, 865, 1105
Tsp45I	GTSAC	1 cut(s)	521
TspDTI	ATGAA	2 cut(s)	65, 392
TspGWI	ACGGA	2 cut(s)	808, 1116
TspRI	CASTG	1 cut(s)	1086
Tth111I	GACNNNGTC	2 cut(s)	605, 1118
Van91I	CCANNNNNTGG	1 cut(s)	518
VpaK11BI	GGWCC	4 cut(s)	118, 531, 583, 1237
XapI	RAATTY	1 cut(s)	990
XmiI	GTMKAC	1 cut(s)	474
XmnI	GAANNNNTTC	1 cut(s)	311
ZraI	GACGTC	1 cut(s)	1120

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.