Rw5G049560

Serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0020887
Physical Location & Seq
Reverse (-)
86056931 .. 86059821
2891 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G049560.1

Sequence Viewer

Length: 528 bp
ATGGATTCGGTCCCGTCGAACTCGCTTGGGAACCTCGACGAGCAGATTGCTCAGCTCATGCAGTGCAAGCCTTTGTCCGAACAAGAGGTAAGGGGGCTCTGTGAAAAGGCTAAGGAGATATTGATGGAAGAAAGCAACGTTCAGCCTGTGAAAAGCCCAGTAACTATTTGCGGTGATATTCATGGACAATTTCATGATCTTGCCGAGCTTTTCCGTATTGGAGGAAAGTGTCCAGATACCAATTATTTGTTTATGGGAGATTATGTGGATCGTGGCTATTATTCCGTTGAAACTGTAACTCTTTTGGTGGCCTTGAAGGTTCGTTACTCTCAACGTATAACTATCCTAAGAGGGAATCATGAAAGTCGTCAGATTACTCAAGTTTATGGTTTTTATGACGAGTGCTTACGGAAGTATGGTAATGCCAATGTTTGGAAGACCTTTACTGATTTATTTGACTATTTTCCCCTGACAGCTTTGGTTAGTATAATTTTTCAGTTATTTATGATGCTTAAATATTTAATGTAG

Protein Analysis

175

Amino Acids

20.16

Weight (kDa)

5.38

Isoelectric Point (pI)

33.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 53 - 163 2.6e-26 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0020887)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42500 AT2G42500 AT2G42500
pyrus_communis pycom03g00740
rosa_wichuraiana Rw5G049560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 432
AciI CCGC 1 cut(s) 171
AclI AACGTT 1 cut(s) 138
AclWI GGATC 1 cut(s) 276
AfiI CCNNNNNNNGG 1 cut(s) 432
AgsI TTSAA 2 cut(s) 290, 316
AluBI AGCT 3 cut(s) 55, 208, 476
AluI AGCT 3 cut(s) 55, 208, 476
AlwI GGATC 1 cut(s) 276
AoxI GGCC 1 cut(s) 309
AspS9I GGNCC 1 cut(s) 10
AsuHPI GGTGA 1 cut(s) 185
AvaII GGWCC 1 cut(s) 10
BanII GRGCYC 1 cut(s) 99
BarI GAAGNNNNNNTAC 2 cut(s) 308, 340
BbsI GAAGAC 1 cut(s) 443
BccI CCATC 1 cut(s) 118
BcgI CGANNNNNNTGC 2 cut(s) 29, 63
BlpI GCTNAGC 1 cut(s) 51
Bme18I GGWCC 1 cut(s) 10
BmgT120I GGNCC 1 cut(s) 10
BmiI GGNNCC 2 cut(s) 12, 32
BmrI ACTGGG 1 cut(s) 152
BmsI GCATC 1 cut(s) 498
BmuI ACTGGG 1 cut(s) 152
BpiI GAAGAC 1 cut(s) 443
Bpu10I CCTNAGC 1 cut(s) 111
Bpu1102I GCTNAGC 1 cut(s) 51
BpuEI CTTGAG 1 cut(s) 363
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 1 cut(s) 432
Bse1I ACTGG 1 cut(s) 158
BseLI CCNNNNNNNGG 1 cut(s) 432
BseMII CTCAG 1 cut(s) 65
BseNI ACTGG 1 cut(s) 158
BshFI GGCC 1 cut(s) 311
BslI CCNNNNNNNGG 1 cut(s) 432
BsnI GGCC 1 cut(s) 311
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 2 cut(s) 196, 268
Bsp1720I GCTNAGC 1 cut(s) 51
BspACI CCGC 1 cut(s) 171
BspANI GGCC 1 cut(s) 311
BspCNI CTCAG 1 cut(s) 64
BspHI TCATGA 2 cut(s) 193, 358
BspLI GGNNCC 2 cut(s) 12, 32
BspPI GGATC 1 cut(s) 276
BsrI ACTGG 1 cut(s) 158
BssMI GATC 2 cut(s) 196, 268
Bst4CI ACNGT 1 cut(s) 295
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 3 cut(s) 51, 111, 347
BstKTI GATC 2 cut(s) 199, 271
BstMBI GATC 2 cut(s) 196, 268
BstMWI GCNNNNNNNGC 1 cut(s) 67
BstV2I GAAGAC 1 cut(s) 443
BsuRI GGCC 1 cut(s) 311
BtsI GCAGTG 1 cut(s) 68
BtsIMutI CAGTG 1 cut(s) 68
Cac8I GCNNGC 1 cut(s) 68
CciI TCATGA 2 cut(s) 193, 358
Cfr13I GGNCC 1 cut(s) 10
CviAII CATG 4 cut(s) 58, 182, 194, 359
DdeI CTNAG 3 cut(s) 51, 111, 347
DpnI GATC 2 cut(s) 198, 270
DpnII GATC 2 cut(s) 196, 268
Eco24I GRGCYC 1 cut(s) 99
Eco47I GGWCC 1 cut(s) 10
EcoT38I GRGCYC 1 cut(s) 99
FaeI CATG 4 cut(s) 61, 185, 197, 362
FatI CATG 4 cut(s) 57, 181, 193, 358
FriOI GRGCYC 1 cut(s) 99
HaeIII GGCC 1 cut(s) 311
Hin1II CATG 4 cut(s) 61, 185, 197, 362
HinfI GANTC 2 cut(s) 5, 355
HphI GGTGA 1 cut(s) 185
Hpy188I TCNGA 2 cut(s) 79, 372
Hpy188III TCNNGA 3 cut(s) 194, 233, 359
Hpy99I CGWCG 2 cut(s) 19, 41
HpyAV CCTTC 1 cut(s) 310
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4IV ACGT 2 cut(s) 138, 334
HpyCH4V TGCA 2 cut(s) 61, 66
HpyF10VI GCNNNNNNNGC 1 cut(s) 67
HpyF3I CTNAG 3 cut(s) 51, 111, 347
HpySE526I ACGT 2 cut(s) 138, 334
Hsp92II CATG 4 cut(s) 61, 185, 197, 362
Kzo9I GATC 2 cut(s) 196, 268
LpnPI CCDG 4 cut(s) 159, 171, 246, 482
LweI GCATC 1 cut(s) 498
MaeII ACGT 2 cut(s) 138, 334
MaeIII GTNAC 3 cut(s) 160, 295, 323
MalI GATC 2 cut(s) 198, 270
MboI GATC 2 cut(s) 196, 268
MboII GAAGA 2 cut(s) 140, 448
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 3 cut(s) 188, 241, 489
MnlI CCTC 4 cut(s) 44, 79, 215, 344
MseI TTAA 2 cut(s) 513, 521
MwoI GCNNNNNNNGC 1 cut(s) 67
NdeII GATC 2 cut(s) 196, 268
NlaIII CATG 4 cut(s) 61, 185, 197, 362
NlaIV GGNNCC 2 cut(s) 12, 32
NmeAIII GCCGAG 1 cut(s) 229
PagI TCATGA 2 cut(s) 193, 358
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PfeI GAWTC 2 cut(s) 5, 355
PflMI CCANNNNNTGG 1 cut(s) 432
Psp1406I AACGTT 1 cut(s) 138
PspN4I GGNNCC 2 cut(s) 12, 32
PspPI GGNCC 1 cut(s) 10
SaqAI TTAA 2 cut(s) 513, 521
Sau3AI GATC 2 cut(s) 196, 268
Sau96I GGNCC 1 cut(s) 10
SduI GDGCHC 1 cut(s) 99
SetI ASST 9 cut(s) 36, 57, 90, 141, 210, 321, 337, 443, 478
SfaNI GCATC 1 cut(s) 498
SinI GGWCC 1 cut(s) 10
SmlI CTYRAG 1 cut(s) 378
SmoI CTYRAG 1 cut(s) 378
Sse9I AATT 3 cut(s) 188, 241, 489
SsiI CCGC 1 cut(s) 171
SspI AATATT 1 cut(s) 518
TaaI ACNGT 1 cut(s) 295
TaiI ACGT 2 cut(s) 141, 337
TaqI TCGA 2 cut(s) 17, 36
TasI AATT 3 cut(s) 188, 241, 489
TfiI GAWTC 2 cut(s) 5, 355
Tru1I TTAA 2 cut(s) 513, 521
Tru9I TTAA 2 cut(s) 513, 521
TscAI CASTG 1 cut(s) 68
TspDTI ATGAA 3 cut(s) 170, 182, 375
TspGWI ACGGA 3 cut(s) 203, 274, 424
TspRI CASTG 1 cut(s) 68
Van91I CCANNNNNTGG 1 cut(s) 432
VpaK11BI GGWCC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.