Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0016603

Physical Location & Seq

Genomic Coordinates Forward (+)

29678158 .. 29680600

Gene Structure 2443 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G024210.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1281 bp

ATGAGGCCACAGCCCTCATCAAAGCCATGGAGACCATTCACAGCGAAGTATTGCTGCTCGGCAGAAGACCAGACATTCTTTGGCAATTTCAGCAGGCCCTGCAGGCCTTCGAGATCAGATTTATCAAGGGATGGTCCTCAGCTACCCTCGTTCCGGCGACTGTCTTTCTCTGATCTCAGTCGTTCTTCCTCGACGCGTATCATGGATGATCTTGCACAGTCTTTCGGGTCTGAGTTGCATGATTTTCAGCTGAGCGAGCTGCGTGGTATAACTCATGATTTCTCGAGTAACTTTTTGTTGGGGGAAGGTGGGTTTGGAACTGTGCATAAGGGCTATATAGATGAGAACTTGAGGCAAGGTTTGAAGCCACAGGCTGTTGCTGTTAAGCTCCTGGACATTGAAGGCTTGCAGGGACACCGCGAATGGCTCGCAGAAGTGATATTTCTTGGGCAGCTGAGGCACCCACATTTGGTCAAATTAATTGGCTATTGTTGTGAGGATGAAGAAAGACTTCTGGTGTATGAGTTTATGTCCAGAGGCAGCTTGGAGAATCACTTATTCAAAAGGCTTTCAATATCATTGCCATGGGGTACAAGAATAAAGATCGCAACAGGAGCAGCCAAAGGCCTTGCTTTCTTGCATGATGCCGAGAAGCCAGTCATTTATCGGGATTTCAAAACTTCAAATGTCTTGCTAGATTCTGATTTTACGGCAAAACTGTCGGATTTCGGACTTGCAAAGATGGGACCTGAAGGATCAGAAACACATGTTACAACAAGAGTAATGGGTACATATGGCTATGCTGCCCCAGAATACGTCTCTACAGGGCAATTGACAACAAAATGTGACATATACAGCTTTGGGGTGGTGCTACTAGAATTGCTTACTGGAAGAAGAGCCATGGACAAATCAAGACCAAAGAGTGAGCAGAATCTCATTGATTGGGCAAAACCTTACCTCACTAGTACTAGAAGGTTACGCTACATTATGGACCCCAGGCTTGCCGGACAGTATTCTGTGAAGGGTGCGAAGGAGATTGCCCTTCTGGCATTACAATGCATAAGTTTGAGCCCTAAAGACAGGCCAAGGATGCCAGCTATTGTTGAAACCCTAGAAGCCGTGCAACTTTTGAAGGACATGGCTGTGACACATGGGCATTGGCCGGCGTCGCCCAAATCCAGTAGAAATGGGGTTTTTGGTGCTAAAGCTAGAGTTGATGGAAATAAAGTTGCGTATATCAGGAAATCCTCTGCAGCTACAACAGTAACTCAGAAATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

426

Amino Acids

47.61

Weight (kDa)

9.35

Isoelectric Point (pI)

37.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	99 - 369	2.2e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	100 - 371	8.4e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016603)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61590
fragaria_vesca	FvH4_5g24390
malus_domestica	MD06G1021900.v1.1
prunus_persica	Prupe.5G016800_v2.0.a1
pyrus_communis	pycom06g01580
rosa_chinensis	RchiOBHm_Chr7g0214781
rosa_laevigata	RLG00000002722
rosa_multiflora	Rmu_sc0021589.1_g000001
rosa_roxburghii	Rroxscaffold_3G00245080
rosa_samantha	Rh7AG284000 Rh7BG274600 Rh7CG303400 Rh7DG287300
rosa_wichuraiana	Rw7G024210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	459
AccII	CGCG	2 cut(s)	196, 420
AciI	CCGC	1 cut(s)	418
AclWI	GGATC	1 cut(s)	763
AcoI	YGGCCR	1 cut(s)	1160
AcuI	CTGAAG	1 cut(s)	771
AcyI	GRCGYC	1 cut(s)	1166
AfaI	GTAC	3 cut(s)	592, 790, 967
AfiI	CCNNNNNNNGG	2 cut(s)	153, 469
AflIII	ACRYGT	2 cut(s)	194, 766
AgsI	TTSAA	8 cut(s)	364, 401, 562, 573, 676, 684, 1106, 1132
AhlI	ACTAGT	1 cut(s)	962
AjnI	CCWGG	2 cut(s)	390, 995
AjuI	GAANNNNNNNTTGG	2 cut(s)	297, 329
Alw26I	GTCTC	2 cut(s)	25, 823
AlwI	GGATC	1 cut(s)	763
AlwNI	CAGNNNCTG	1 cut(s)	99
Ama87I	CYCGRG	1 cut(s)	283
AoxI	GGCC	6 cut(s)	5, 95, 104, 625, 1082, 1160
ApeKI	GCWGC	7 cut(s)	54, 259, 451, 540, 617, 803, 1253
AseI	ATTAAT	1 cut(s)	479
Asp700I	GAANNNNTTC	1 cut(s)	510
AspS9I	GGNCC	4 cut(s)	96, 134, 746, 991
AvaI	CYCGRG	1 cut(s)	283
AvaII	GGWCC	3 cut(s)	134, 746, 991
BanI	GGYRCC	1 cut(s)	459
BanII	GRGCYC	1 cut(s)	1073
BbsI	GAAGAC	1 cut(s)	72
BbvCI	CCTCAGC	2 cut(s)	138, 455
BbvI	GCAGC	7 cut(s)	41, 246, 463, 552, 629, 790, 1265
BccI	CCATC	3 cut(s)	125, 736, 1211
BceAI	ACGGC	2 cut(s)	726, 1103
BcgI	CGANNNNNNTGC	2 cut(s)	702, 736
BciT130I	CCWGG	2 cut(s)	392, 997
BcoDI	GTCTC	2 cut(s)	25, 823
BcuI	ACTAGT	1 cut(s)	962
BfaI	CTAG	6 cut(s)	695, 875, 963, 969, 1112, 1209
BfmI	CTRYAG	3 cut(s)	100, 822, 1251
BglI	GCCNNNNNGGC	2 cut(s)	103, 1046
BisI	GCNGC	7 cut(s)	55, 260, 452, 541, 618, 804, 1254
BlpI	GCTNAGC	1 cut(s)	251
BlsI	GCNGC	7 cut(s)	56, 261, 453, 542, 619, 805, 1255
BmcAI	AGTACT	1 cut(s)	967
Bme1390I	CCNGG	2 cut(s)	392, 997
Bme18I	GGWCC	3 cut(s)	134, 746, 991
BmeT110I	CYCGRG	1 cut(s)	283
BmgT120I	GGNCC	4 cut(s)	96, 134, 746, 991
BmiI	GGNNCC	3 cut(s)	461, 747, 993
BmrFI	CCNGG	2 cut(s)	392, 997
BmsI	GCATC	2 cut(s)	634, 1080
BpiI	GAAGAC	1 cut(s)	72
Bpu10I	CCTNAGC	2 cut(s)	138, 455
Bpu1102I	GCTNAGC	1 cut(s)	251
BpuEI	CTTGAG	1 cut(s)	370
BsaHI	GRCGYC	1 cut(s)	1166
BsaI	GGTCTC	1 cut(s)	25
BsaJI	CCNNGG	5 cut(s)	26, 584, 900, 995, 1085
Bsc4I	CCNNNNNNNGG	2 cut(s)	153, 469
Bse118I	RCCGGY	1 cut(s)	1162
Bse1I	ACTGG	3 cut(s)	656, 892, 1179
Bse3DI	GCAATG	1 cut(s)	578
BseBI	CCWGG	2 cut(s)	392, 997
BseDI	CCNNGG	5 cut(s)	26, 584, 900, 995, 1085
BseGI	GGATG	4 cut(s)	136, 211, 505, 1095
BseLI	CCNNNNNNNGG	2 cut(s)	153, 469
BseMI	GCAATG	1 cut(s)	578
BseMII	CTCAG	5 cut(s)	152, 190, 222, 242, 446
BseNI	ACTGG	3 cut(s)	656, 892, 1179
BseXI	GCAGC	7 cut(s)	41, 246, 463, 552, 629, 790, 1265
Bsh1236I	CGCG	2 cut(s)	196, 420
BshFI	GGCC	6 cut(s)	7, 97, 106, 627, 1084, 1162
BshNI	GGYRCC	1 cut(s)	459
BsiHKCI	CYCGRG	1 cut(s)	283
BsiSI	CCGG	3 cut(s)	154, 1005, 1163
BslFI	GGGAC	2 cut(s)	426, 759
BslI	CCNNNNNNNGG	2 cut(s)	153, 469
BsmAI	GTCTC	2 cut(s)	25, 823
BsmBI	CGTCTC	1 cut(s)	823
BsmFI	GGGAC	2 cut(s)	426, 759
BsnI	GGCC	6 cut(s)	7, 97, 106, 627, 1084, 1162
Bso31I	GGTCTC	1 cut(s)	25
BsoBI	CYCGRG	1 cut(s)	283
Bsp1286I	GDGCHC	1 cut(s)	1073
Bsp143I	GATC	5 cut(s)	113, 172, 208, 603, 755
Bsp1720I	GCTNAGC	1 cut(s)	251
Bsp19I	CCATGG	3 cut(s)	26, 584, 900
BspACI	CCGC	1 cut(s)	418
BspANI	GGCC	6 cut(s)	7, 97, 106, 627, 1084, 1162
BspCNI	CTCAG	5 cut(s)	151, 189, 223, 243, 447
BspFNI	CGCG	2 cut(s)	196, 420
BspHI	TCATGA	1 cut(s)	274
BspLI	GGNNCC	3 cut(s)	461, 747, 993
BspMAI	CTGCAG	2 cut(s)	104, 1255
BspPI	GGATC	1 cut(s)	763
BspQI	GCTCTTC	1 cut(s)	889
BspT107I	GGYRCC	1 cut(s)	459
BspTNI	GGTCTC	1 cut(s)	25
BsrDI	GCAATG	1 cut(s)	578
BsrFI	RCCGGY	1 cut(s)	1162
BsrI	ACTGG	3 cut(s)	656, 892, 1179
BssAI	RCCGGY	1 cut(s)	1162
BssECI	CCNNGG	5 cut(s)	26, 584, 900, 995, 1085
BssMI	GATC	5 cut(s)	113, 172, 208, 603, 755
BssNI	GRCGYC	1 cut(s)	1166
BssT1I	CCWWGG	4 cut(s)	26, 584, 900, 1085
Bst2UI	CCWGG	2 cut(s)	392, 997
Bst4CI	ACNGT	6 cut(s)	162, 219, 322, 720, 1011, 1264
Bst6I	CTCTTC	1 cut(s)	889
BstACI	GRCGYC	1 cut(s)	1166
BstAPI	GCANNNNNTGC	1 cut(s)	99
BstC8I	GCNNGC	8 cut(s)	95, 104, 257, 407, 429, 1002, 1095, 1164
BstDEI	CTNAG	6 cut(s)	138, 176, 231, 251, 455, 1269
BstDSI	CCRYGG	3 cut(s)	26, 584, 900
BstF5I	GGATG	4 cut(s)	136, 211, 505, 1095
BstFNI	CGCG	2 cut(s)	196, 420
BstKTI	GATC	5 cut(s)	116, 175, 211, 606, 758
BstMAI	GTCTC	2 cut(s)	25, 823
BstMBI	GATC	5 cut(s)	113, 172, 208, 603, 755
BstMWI	GCNNNNNNNGC	9 cut(s)	90, 99, 103, 256, 457, 614, 1046, 1090, 1168
BstNI	CCWGG	2 cut(s)	392, 997
BstNSI	RCATGY	1 cut(s)	770
BstSCI	CCNGG	2 cut(s)	390, 995
BstSFI	CTRYAG	3 cut(s)	100, 822, 1251
BstUI	CGCG	2 cut(s)	196, 420
BstV1I	GCAGC	7 cut(s)	41, 246, 463, 552, 629, 790, 1265
BstV2I	GAAGAC	1 cut(s)	72
BsuRI	GGCC	6 cut(s)	7, 97, 106, 627, 1084, 1162
BtgI	CCRYGG	3 cut(s)	26, 584, 900
BtsCI	GGATG	4 cut(s)	136, 211, 505, 1095
Cac8I	GCNNGC	8 cut(s)	95, 104, 257, 407, 429, 1002, 1095, 1164
CaiI	CAGNNNCTG	1 cut(s)	99
CciI	TCATGA	1 cut(s)	274
Cfr10I	RCCGGY	1 cut(s)	1162
Cfr13I	GGNCC	4 cut(s)	96, 134, 746, 991
CseI	GACGC	2 cut(s)	202, 1155
Csp6I	GTAC	3 cut(s)	591, 789, 966
CviQI	GTAC	3 cut(s)	591, 789, 966
DdeI	CTNAG	6 cut(s)	138, 176, 231, 251, 455, 1269
DpnI	GATC	5 cut(s)	115, 174, 210, 605, 757
DpnII	GATC	5 cut(s)	113, 172, 208, 603, 755
EaeI	YGGCCR	1 cut(s)	1160
Eam1104I	CTCTTC	1 cut(s)	889
EarI	CTCTTC	1 cut(s)	889
Eco130I	CCWWGG	4 cut(s)	26, 584, 900, 1085
Eco147I	AGGCCT	2 cut(s)	106, 627
Eco24I	GRGCYC	1 cut(s)	1073
Eco31I	GGTCTC	1 cut(s)	25
Eco47I	GGWCC	3 cut(s)	134, 746, 991
Eco57I	CTGAAG	1 cut(s)	771
Eco88I	CYCGRG	1 cut(s)	283
EcoO109I	RGGNCCY	2 cut(s)	96, 746
EcoRII	CCWGG	2 cut(s)	390, 995
EcoT14I	CCWWGG	4 cut(s)	26, 584, 900, 1085
EcoT22I	ATGCAT	1 cut(s)	1061
EcoT38I	GRGCYC	1 cut(s)	1073
ErhI	CCWWGG	4 cut(s)	26, 584, 900, 1085
Esp3I	CGTCTC	1 cut(s)	823
FalI	AAGNNNNNCTT	2 cut(s)	495, 527
FaqI	GGGAC	2 cut(s)	426, 759
FauNDI	CATATG	1 cut(s)	793
Fnu4HI	GCNGC	7 cut(s)	55, 260, 452, 541, 618, 804, 1254
FokI	GGATG	4 cut(s)	143, 218, 512, 1102
FriOI	GRGCYC	1 cut(s)	1073
Fsp4HI	GCNGC	7 cut(s)	55, 260, 452, 541, 618, 804, 1254
FspBI	CTAG	6 cut(s)	695, 875, 963, 969, 1112, 1209
GluI	GCNGC	7 cut(s)	55, 260, 452, 541, 618, 804, 1254
HaeIII	GGCC	6 cut(s)	7, 97, 106, 627, 1084, 1162
HapII	CCGG	3 cut(s)	154, 1005, 1163
HgaI	GACGC	2 cut(s)	202, 1155
Hin1I	GRCGYC	1 cut(s)	1166
HinfI	GANTC	3 cut(s)	550, 698, 931
HpaII	CCGG	3 cut(s)	154, 1005, 1163
Hpy188I	TCNGA	8 cut(s)	118, 172, 232, 703, 724, 731, 760, 1272
Hpy188III	TCNNGA	8 cut(s)	111, 275, 283, 534, 668, 912, 1240, 1278
Hpy99I	CGWCG	2 cut(s)	196, 1171
HpyAV	CCTTC	9 cut(s)	117, 299, 395, 746, 966, 1015, 1024, 1052, 1126
HpyCH4III	ACNGT	6 cut(s)	162, 219, 322, 720, 1011, 1264
HpyCH4IV	ACGT	1 cut(s)	816
HpyF10VI	GCNNNNNNNGC	9 cut(s)	90, 99, 103, 256, 457, 614, 1046, 1090, 1168
HpyF3I	CTNAG	6 cut(s)	138, 176, 231, 251, 455, 1269
HpySE526I	ACGT	1 cut(s)	816
Hsp92I	GRCGYC	1 cut(s)	1166
KroI	GCCGGC	1 cut(s)	1162
KroNI	GCCGGC	1 cut(s)	1164
Kzo9I	GATC	5 cut(s)	113, 172, 208, 603, 755
LguI	GCTCTTC	1 cut(s)	889
LmnI	GCTCC	2 cut(s)	393, 614
Lsp1109I	GCAGC	7 cut(s)	41, 246, 463, 552, 629, 790, 1265
LweI	GCATC	2 cut(s)	634, 1080
MaeI	CTAG	6 cut(s)	695, 875, 963, 969, 1112, 1209
MaeII	ACGT	1 cut(s)	816
MaeIII	GTNAC	6 cut(s)	287, 769, 845, 975, 1144, 1264
MalI	GATC	5 cut(s)	115, 174, 210, 605, 757
MboI	GATC	5 cut(s)	113, 172, 208, 603, 755
MboII	GAAGA	5 cut(s)	77, 177, 515, 903, 906
MfeI	CAATTG	1 cut(s)	830
MhlI	GDGCHC	1 cut(s)	1073
MluCI	AATT	5 cut(s)	85, 476, 480, 830, 878
MluI	ACGCGT	1 cut(s)	194
MmeI	TCCRAC	1 cut(s)	702
Mph1103I	ATGCAT	1 cut(s)	1061
MroNI	GCCGGC	1 cut(s)	1162
MroXI	GAANNNNTTC	1 cut(s)	510
MseI	TTAA	2 cut(s)	384, 479
MslI	CAYNNNNRTG	3 cut(s)	583, 1054, 1142
MspA1I	CMGCKG	2 cut(s)	250, 454
MspI	CCGG	3 cut(s)	154, 1005, 1163
MspR9I	CCNGG	2 cut(s)	392, 997
MunI	CAATTG	1 cut(s)	830
MvaI	CCWGG	2 cut(s)	392, 997
MvnI	CGCG	2 cut(s)	196, 420
MwoI	GCNNNNNNNGC	9 cut(s)	90, 99, 103, 256, 457, 614, 1046, 1090, 1168
NaeI	GCCGGC	1 cut(s)	1164
NcoI	CCATGG	3 cut(s)	26, 584, 900
NdeI	CATATG	1 cut(s)	793
NdeII	GATC	5 cut(s)	113, 172, 208, 603, 755
NgoMIV	GCCGGC	1 cut(s)	1162
NlaIV	GGNNCC	3 cut(s)	461, 747, 993
NmeAIII	GCCGAG	2 cut(s)	38, 673
NmuCI	GTSAC	2 cut(s)	845, 1144
NsiI	ATGCAT	1 cut(s)	1061
NspI	RCATGY	1 cut(s)	770
PaeR7I	CTCGAG	1 cut(s)	283
PagI	TCATGA	1 cut(s)	274
PceI	AGGCCT	2 cut(s)	106, 627
PciI	ACATGT	1 cut(s)	766
PciSI	GCTCTTC	1 cut(s)	889
PdiI	GCCGGC	1 cut(s)	1164
PdmI	GAANNNNTTC	1 cut(s)	510
PfeI	GAWTC	3 cut(s)	550, 698, 931
PfoI	TCCNGGA	1 cut(s)	390
PkrI	GCNGC	7 cut(s)	56, 261, 453, 542, 619, 805, 1255
PpuMI	RGGWCCY	1 cut(s)	746
PscI	ACATGT	1 cut(s)	766
PshBI	ATTAAT	1 cut(s)	479
Psp5II	RGGWCCY	1 cut(s)	746
Psp6I	CCWGG	2 cut(s)	390, 995
PspGI	CCWGG	2 cut(s)	390, 995
PspN4I	GGNNCC	3 cut(s)	461, 747, 993
PspPI	GGNCC	4 cut(s)	96, 134, 746, 991
PspPPI	RGGWCCY	1 cut(s)	746
PstI	CTGCAG	2 cut(s)	104, 1255
PstNI	CAGNNNCTG	1 cut(s)	99
PvuII	CAGCTG	2 cut(s)	250, 454
RsaI	GTAC	3 cut(s)	592, 790, 967
RsaNI	GTAC	3 cut(s)	591, 789, 966
RseI	CAYNNNNRTG	3 cut(s)	583, 1054, 1142
SapI	GCTCTTC	1 cut(s)	889
SaqAI	TTAA	2 cut(s)	384, 479
SatI	GCNGC	7 cut(s)	55, 260, 452, 541, 618, 804, 1254
Sau3AI	GATC	5 cut(s)	113, 172, 208, 603, 755
Sau96I	GGNCC	4 cut(s)	96, 134, 746, 991
SbfI	CCTGCAGG	1 cut(s)	104
ScaI	AGTACT	1 cut(s)	967
ScrFI	CCNGG	2 cut(s)	392, 997
SdaI	CCTGCAGG	1 cut(s)	104
SduI	GDGCHC	1 cut(s)	1073
SfaNI	GCATC	2 cut(s)	634, 1080
SfcI	CTRYAG	3 cut(s)	100, 822, 1251
SfiI	GGCCNNNNNGGCC	1 cut(s)	103
Sfr274I	CTCGAG	1 cut(s)	283
SinI	GGWCC	3 cut(s)	134, 746, 991
SlaI	CTCGAG	1 cut(s)	283
SmiMI	CAYNNNNRTG	3 cut(s)	583, 1054, 1142
SmlI	CTYRAG	2 cut(s)	283, 349
SmoI	CTYRAG	2 cut(s)	283, 349
SpeI	ACTAGT	1 cut(s)	962
Sse8387I	CCTGCAGG	1 cut(s)	104
Sse9I	AATT	5 cut(s)	85, 476, 480, 830, 878
SseBI	AGGCCT	2 cut(s)	106, 627
SsiI	CCGC	1 cut(s)	418
SspMI	CTAG	6 cut(s)	695, 875, 963, 969, 1112, 1209
StuI	AGGCCT	2 cut(s)	106, 627
StyD4I	CCNGG	2 cut(s)	390, 995
StyI	CCWWGG	4 cut(s)	26, 584, 900, 1085
TaaI	ACNGT	6 cut(s)	162, 219, 322, 720, 1011, 1264
TaiI	ACGT	1 cut(s)	819
TaqI	TCGA	3 cut(s)	110, 191, 284
TasI	AATT	5 cut(s)	85, 476, 480, 830, 878
TatI	WGTACW	1 cut(s)	965
TfiI	GAWTC	3 cut(s)	550, 698, 931
Tru1I	TTAA	2 cut(s)	384, 479
Tru9I	TTAA	2 cut(s)	384, 479
TseFI	GTSAC	2 cut(s)	845, 1144
TseI	GCWGC	7 cut(s)	54, 259, 451, 540, 617, 803, 1253
Tsp45I	GTSAC	2 cut(s)	845, 1144
TspDTI	ATGAA	1 cut(s)	516
VpaK11BI	GGWCC	3 cut(s)	134, 746, 991
VspI	ATTAAT	1 cut(s)	479
XceI	RCATGY	1 cut(s)	770
XcmI	CCANNNNNNNNNTGG	2 cut(s)	77, 541
XhoI	CTCGAG	1 cut(s)	283
XmnI	GAANNNNTTC	1 cut(s)	510
XspI	CTAG	6 cut(s)	695, 875, 963, 969, 1112, 1209
ZrmI	AGTACT	1 cut(s)	967
Zsp2I	ATGCAT	1 cut(s)	1061

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.